Influence of Short and Long Hyperglycemia on Cardioprotection by Remote Ischemic Preconditioning—A Translational Approach
Abstract
:1. Introduction
2. Results
2.1. Animal Characteristics
2.2. Infarct Size Measurement
2.3. Cardiac Function and Glucose Values
3. Discussion
3.1. RIPC Plasma Transfer under Normoglycemia
3.2. Influence of Short and Prolonged Hyperglycemia on Transferred RIPC Plasma
4. Materials and Methods
4.1. Part 1: RIPC-Plasma Collection in Volunteers
4.2. Part 2: (a) Experimental Setting—Langendorff Isolated Heart
4.3. Part 2: (b) Experimental Protocol
4.4. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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n | Body Weight (g) | Heart Weight Wet (g) | Time of Max. Ischemic Contracture (min) | Level of Max. Ischemic Contracture (mmHg) | |
---|---|---|---|---|---|
Con | 4 | 325 ± 9 | 1.17 ± 0.09 | 20 ± 2 | 62 ± 16 |
RIPC | 4 | 293 ± 16 | 1.05 ± 0.03 | 22 ± 3 | 60 ± 9 |
HGs Con | 5 | 312 ± 21 | 1.16 ± 0.04 | 19 ± 2 | 75 ± 8 |
HGs RIPC | 4 | 312 ± 21 | 1.16 ± 0.04 | 19 ± 2 | 75 ± 8 |
HGl Con | 4 | 309 ± 19 | 1.10 ± 0.03 | 19 ± 3 | 79 ± 10 |
HGl RIPC | 4 | 297 ± 28 | 1.07 ± 0.02 | 19 ± 1 | 67 ± 22 |
Baseline | PC | Reperfusion | ||
---|---|---|---|---|
30 | 60 | |||
Heart Rate (bpm) | ||||
Con | 277 ± 13 | 259 ± 25 | 246 ± 80 | 218 ± 69 |
RIPC | 312 ± 43 | 295 ± 46 | 237 ± 94 | 225 ± 91 |
HGs Con | 305 ± 32 | 300 ± 30 | 309 ± 55 | 238 ± 57 |
HGs RIPC | 304 ± 36 | 282 ± 26 | 290 ± 42 | 263 ± 81 |
HGl Con | 282 ± 11 | 279 ± 9 | 235 ± 28 | 236 ± 29 |
HGl RIPC | 266 ± 5 | 269 ± 6 | 273 ± 20 | 226 ± 42 |
Left Ventricular Developed Pressure (mmHg) | ||||
Con | 130 ± 12 | 134 ± 10 | 15 ± 11 * | 25 ± 16 * |
RIPC | 130 ± 4 | 145 ± 19 | 51 ± 19 * | 51 ± 7 * |
HGs Con | 137 ± 8 | 139 ± 12 | 28 ± 26 * | 28 ± 12 * |
HGs RIPC | 125 ± 14 | 125 ± 11 | 24 ± 8 * | 30 ± 10 * |
HGl Con | 131 ± 17 | 125 ± 18 | 19 ± 14 * | 15 ± 10 * |
HGl RIPC | 141 ± 3 | 138 ± 5 | 10 ± 8 * | 27 ± 12 * |
Left Ventricular End-Diastolic Pressure (mmHg) | ||||
Con | 5 ± 1 | 5 ± 1 | 86 ± 8 * | 76 ± 7 * |
RIPC | 4 ± 1 | 3 ± 1 | 74 ± 4 *§ | 71 ± 7 * |
HGs Con | 5 ± 1 | 5 ± 1 | 81 ± 8 * | 73 ± 15 * |
HGs RIPC | 4 ± 1 | 4 ± 1 | 75 ± 5 *§ | 66 ± 3 * |
HGl Con | 2 ± 1 | 3 ± 4 | 100 ± 13 * | 93 ± 14 * |
HGl RIPC | 2 ± 1 | 2 ± 1 | 104 ± 16 * | 89 ± 14 * |
Coronary Flow (mL/min) | ||||
Con | 14 ± 2 | 14 ± 2 | 6 ± 1 * | 6 ± 1 * |
RIPC | 13 ± 1 | 13 ± 2 | 6 ± 3 * | 6 ± 2 * |
HGs Con | 15 ± 2 | 15 ± 2 | 8 ± 2 * | 7 ± 2 * |
HGs RIPC | 16 ± 3 | 15 ± 2 | 7 ± 1 * | 7 ± 1 * |
HGl Con | 16 ± 1 | 17 ± 3 | 5 ± 2 * | 5 ± 2 * |
HGl RIPC | 15 ± 2 | 15 ± 2 | 5 ± 1 * | 6 ± 1 * |
Glucose Levels (mg/dL) | ||||
Con | 196 ± 1 | 200 ± 6 | 193 ± 3 | 196 ± 3 |
RIPC | 196 ± 3 | 200 ± 3 | 198 ± 6 | 199 ± 4 |
HGs Con | 194 ± 5 | 419 ± 27 * | 196 ± 3 # | 196 ± 3 # |
HGs RIPC | 196 ± 3 | 408 ± 23 * | 197 ± 2 # | 197 ± 2 # |
HGl Con | 195 ± 2 | 358 ± 19 * | 382 ± 40 * | 396 ± 54 * |
HGl RIPC | 195 ± 4 | 358 ± 4 * | 367 ± 29 * | 356 ± 23 * |
n | Age (Years) | Height (cm) | Body Weight (kg) | BMI (kg/m2) | |
---|---|---|---|---|---|
Male | 10 | 25 ± 3 | 184 ± 5 | 84 ± 12 | 24.6 ± 3.4 |
Criteria for inclusion |
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Criteria for exclusion |
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Feige, K.; Roth, S.; M’Pembele, R.; Galow, A.; Koenig, S.; Stroethoff, M.; Raupach, A.; Lurati Buse, G.; Mathes, A.M.; Hollmann, M.W.; et al. Influence of Short and Long Hyperglycemia on Cardioprotection by Remote Ischemic Preconditioning—A Translational Approach. Int. J. Mol. Sci. 2022, 23, 14557. https://doi.org/10.3390/ijms232314557
Feige K, Roth S, M’Pembele R, Galow A, Koenig S, Stroethoff M, Raupach A, Lurati Buse G, Mathes AM, Hollmann MW, et al. Influence of Short and Long Hyperglycemia on Cardioprotection by Remote Ischemic Preconditioning—A Translational Approach. International Journal of Molecular Sciences. 2022; 23(23):14557. https://doi.org/10.3390/ijms232314557
Chicago/Turabian StyleFeige, Katharina, Sebastian Roth, René M’Pembele, Anna Galow, Sarah Koenig, Martin Stroethoff, Annika Raupach, Giovanna Lurati Buse, Alexander M. Mathes, Markus W. Hollmann, and et al. 2022. "Influence of Short and Long Hyperglycemia on Cardioprotection by Remote Ischemic Preconditioning—A Translational Approach" International Journal of Molecular Sciences 23, no. 23: 14557. https://doi.org/10.3390/ijms232314557