Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells
Abstract
:1. Introduction
2. Results
2.1. The Feasibility of Tricine in Cryopreservation
2.2. The Ability of Osmotic Regulation
2.3. Reducing the Mechanical Damage from Ice
2.4. Resistance to Oxidative Damage
2.5. The Properties of Thawed RBCs
2.6. Comparing CPAs by Mathematical Models
3. Discussion
4. Materials and Methods
4.1. RBCs Preparation and Recovery Test
4.2. Biocompatibility Test
4.3. SEM Analysis
4.4. Cryopreservation of RBCs
4.5. Osmotic Regulation Test
4.6. Splat Assay
4.7. DSC Test
4.8. Antioxidant Assay
4.9. ESR Analysis
4.10. ATPase Activities
4.11. Content of Hb
4.12. Mathematical Model Analysis
4.13. Data Analysis
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Types of CPAs | Concentration (%wt) | Thawed RBCs Recovery (%) | Biocompatibility(%) |
---|---|---|---|
Glycerol | 20.0 | 89.5 | 74.9 |
HES | 13.0 | 39.7 | 97.5 |
Tricine | 6.0 | 81.2 | 95.8 |
Types of CPAs | Closeness Coefficient | Rank |
---|---|---|
Glycerol | 0.622 | 2 |
HES | 0.355 | 3 |
Tricine | 0.853 | 1 |
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Liu, X.; Hu, Y.; Zhang, W.; Yang, D.; Pan, Y.; Ekpo, M.D.; Xie, J.; Zhao, R.; Tan, S. Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells. Int. J. Mol. Sci. 2022, 23, 8462. https://doi.org/10.3390/ijms23158462
Liu X, Hu Y, Zhang W, Yang D, Pan Y, Ekpo MD, Xie J, Zhao R, Tan S. Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells. International Journal of Molecular Sciences. 2022; 23(15):8462. https://doi.org/10.3390/ijms23158462
Chicago/Turabian StyleLiu, Xiangjian, Yuying Hu, Wenqian Zhang, Deyi Yang, Yuxin Pan, Marlene Davis Ekpo, Jingxian Xie, Rui Zhao, and Songwen Tan. 2022. "Tricine as a Novel Cryoprotectant with Osmotic Regulation, Ice Recrystallization Inhibition and Antioxidant Properties for Cryopreservation of Red Blood Cells" International Journal of Molecular Sciences 23, no. 15: 8462. https://doi.org/10.3390/ijms23158462