Overexpressing PhytochromeInteractingFactor 8 of Myrothamnus flabellifolia Enhanced Drought and Salt Tolerance in Arabidopsis
Abstract
:1. Introduction
2. Results
2.1. Isolation and Characterization of MfPIF8
2.2. Overexpressing MfPIF8 Enhanced Tolerance to Drought and Salinity
2.3. Effect of MfPIF8 Overexpression on Antioxidant Metabolism to Arabidopsis
2.4. MfPIF8 Overexpression Promoted Stomatal Closure Induced by Drought
3. Discussion
4. Materials and Methods
4.1. Plant Materials, Growing Conditions, and Stress Treatments
4.2. Cloning and Bioinformatic Analysis of MfPIF8
4.3. Vector Construction and Generation of Transgenic Lines
4.4. Water Loss Rate
4.5. Stomatal Aperture Analysis
4.6. Measurements of Tolerance-Related Physiological and Biochemical Parameters
4.7. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Huang, Z.; Tang, R.; Yi, X.; Xu, W.; Zhu, P.; Jiang, C.-Z. Overexpressing PhytochromeInteractingFactor 8 of Myrothamnus flabellifolia Enhanced Drought and Salt Tolerance in Arabidopsis. Int. J. Mol. Sci. 2022, 23, 8155. https://doi.org/10.3390/ijms23158155
Huang Z, Tang R, Yi X, Xu W, Zhu P, Jiang C-Z. Overexpressing PhytochromeInteractingFactor 8 of Myrothamnus flabellifolia Enhanced Drought and Salt Tolerance in Arabidopsis. International Journal of Molecular Sciences. 2022; 23(15):8155. https://doi.org/10.3390/ijms23158155
Chicago/Turabian StyleHuang, Zhuo, Rong Tang, Xin Yi, Wenxin Xu, Peilei Zhu, and Cai-Zhong Jiang. 2022. "Overexpressing PhytochromeInteractingFactor 8 of Myrothamnus flabellifolia Enhanced Drought and Salt Tolerance in Arabidopsis" International Journal of Molecular Sciences 23, no. 15: 8155. https://doi.org/10.3390/ijms23158155