Next Article in Journal
Mitochondrial Transcription Termination Factor 27 Is Required for Salt Tolerance in Arabidopsis thaliana
Next Article in Special Issue
Mitocryptide-2: Identification of Its Minimum Structure for Specific Activation of FPR2–Possible Receptor Switching from FPR2 to FPR1 by Its Physiological C-terminal Cleavages
Previous Article in Journal
Sexual Dimorphisms, Anti-Hormonal Therapy and Cardiac Arrhythmias
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
IJMS
Volume 22
Issue 3
10.3390/ijms22031462
Review Report
ijms-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
Article
Peer-Review Record

Ordered Clusters of the Complete Oxidative Phosphorylation System in Cardiac Mitochondria

Int. J. Mol. Sci. 2021, 22(3), 1462; https://doi.org/10.3390/ijms22031462
by Semen Nesterov 1,2,3,*, Yury Chesnokov 1,4, Roman Kamyshinsky 1,2,4, Alisa Panteleeva 5, Konstantin Lyamzaev 5, Raif Vasilov 1 and Lev Yaguzhinsky 2,3,5
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2021, 22(3), 1462; https://doi.org/10.3390/ijms22031462
Submission received: 22 December 2020 / Revised: 26 January 2021 / Accepted: 30 January 2021 / Published: 2 February 2021
(This article belongs to the Special Issue Research of Mitochondrial Function, Structure, Dynamics and Intracellular Organization)

Round 1

Reviewer 1 Report

In this study the authors have used cryo-electron tomography to examine the structure and location of OXPHOS complexes and ATP synthase, particularly in relation to each other.  This study adds to previous knowledge obtained with other techniques.

The cryo-EM method appears to be well described, however, the manuscript would benefit from the following information being added to the methods:

  • How were the rats housed and killed?
  • Why were cardiac mitochondria examined?
  • How many rats were used and how many replicates were examined i.e. biological and technical replicates
  • Please go into more detail regarding statistical analysis, particularly for figure S5.

Please add arrows or some other type of markers to figure S1 to show the tubular cristae for clarity.

Figure S5 is not very clear, should there be error bars?  Which bars correspond with which inserts along the top?

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Reviewer 2 Report

This study provides new information the complete oxidative phosphorylation system in cardiac mitochondria. A major revision is suggested.

 

  1. The abstract needs a massive revision and improve. Please address, background, methods and conclusion of this study.
  2. Please address clinical implications of this study.
  3. Please provide details of animal study. How to scarify? Anesthetics used?
  4. How many animals were used? How many repetition of each investigation? Please address.
  5. Please discuss limitations of this study.

Author Response

Please see the attachment

Author Response File: Author Response.pdf

Round 2

Reviewer 2 Report

Paper is fine. Authors made all necessary corrections. Thanks

Back to TopTop