LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases
Abstract
:1. Introduction
2. Results
2.1. Identification and Characteristics of Genes Expressed in EVT Cells from RNA Sequencing
2.2. miR-675-5p Accelerates EVT Invasion
2.3. miR-675-5p Targets GATA2 Directly
2.4. miR-675-5p-Mediated GATA2 Inhibition Accelerates EVT Cell Invasion by Upregulating MMP13 and MMP14
3. Discussion
4. Materials and Methods
4.1. Culture of Cell Lines
4.2. Trophoblast Cell Collection
4.3. RNA Sequencing and Data Analysis
4.4. Transfection of RNA Oligos
4.5. Quantitative Polymerase Chain Reaction (qPCR)
4.6. Western Blotting
4.7. Cell Invasion Assay
4.8. Cell Proliferation Assay
4.9. In Silico Prediction of miRNA Target Genes
4.10. Luciferase Reporter Assay
4.11. Statistics
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
CVT | chorionic villous trophoblast |
DEG | differentially expressed gene |
EVT | extravillous trophoblast |
FBS | fetal bovine serum |
FDR | false discovery rate |
lncRNA | long non-coding RNA |
miRNA | microRNA |
NC | negative control |
ncRNA | non-coding RNA |
PCR | polymerase chain reaction |
siRNA | small interfering RNA |
sncRNA | short non-coding RNA |
UTR | untranslated region |
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Ogoyama, M.; Ohkuchi, A.; Takahashi, H.; Zhao, D.; Matsubara, S.; Takizawa, T. LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases. Int. J. Mol. Sci. 2021, 22, 1237. https://doi.org/10.3390/ijms22031237
Ogoyama M, Ohkuchi A, Takahashi H, Zhao D, Matsubara S, Takizawa T. LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases. International Journal of Molecular Sciences. 2021; 22(3):1237. https://doi.org/10.3390/ijms22031237
Chicago/Turabian StyleOgoyama, Manabu, Akihide Ohkuchi, Hironori Takahashi, Dongwei Zhao, Shigeki Matsubara, and Toshihiro Takizawa. 2021. "LncRNA H19-Derived miR-675-5p Accelerates the Invasion of Extravillous Trophoblast Cells by Inhibiting GATA2 and Subsequently Activating Matrix Metalloproteinases" International Journal of Molecular Sciences 22, no. 3: 1237. https://doi.org/10.3390/ijms22031237