Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density
Abstract
:1. Introduction
2. Results
2.1. Aging-Related Reduction of PSD-95 and HSF1 Is Increased in HD
2.2. Acute or Chronic Reduction of HSF1 Results in Reduced PSD-95 Expression
2.3. HSF1 Directly Binds to HSEs Present in the PSD-95 (Dlg4) Gene and Regulates Its Transcription
2.4. HSF1 Binding to Dlg4 Regulatory Elements Is Impaired in HD
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Mouse Strains
4.3. Human Samples
4.4. Immunoblot Analysis
4.5. Immunohistochemistry & Synapse Density Analyses
4.6. RNA Preparation and RT-qPCR
4.7. siRNA Transfection
4.8. Chromatin Immunoprecipitation
4.9. Luciferase Assays
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Zarate, N.; Intihar, T.A.; Yu, D.; Sawyer, J.; Tsai, W.; Syed, M.; Carlson, L.; Gomez-Pastor, R. Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density. Int. J. Mol. Sci. 2021, 22, 13113. https://doi.org/10.3390/ijms222313113
Zarate N, Intihar TA, Yu D, Sawyer J, Tsai W, Syed M, Carlson L, Gomez-Pastor R. Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density. International Journal of Molecular Sciences. 2021; 22(23):13113. https://doi.org/10.3390/ijms222313113
Chicago/Turabian StyleZarate, Nicole, Taylor A. Intihar, Dahyun Yu, Jacob Sawyer, Wei Tsai, Maha Syed, Luke Carlson, and Rocio Gomez-Pastor. 2021. "Heat Shock Factor 1 Directly Regulates Postsynaptic Scaffolding PSD-95 in Aging and Huntington’s Disease and Influences Striatal Synaptic Density" International Journal of Molecular Sciences 22, no. 23: 13113. https://doi.org/10.3390/ijms222313113