Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture and Treatment
2.2. LDH Assessment
2.3. Animal Model Establishment
2.4. Gut Microbiota Analysis
2.5. Metabolomics Profiling
2.6. Faecal Occult Blood Test
2.7. Intestinal Permeability to FITC-Dextran
2.8. Enzyme-Linked Immunosorbent Assay (ELISA)
2.9. Histological Staining
2.10. Immunohistochemical Staining
2.11. Western Blotting
2.12. Statistical Analysis of Data
3. Results
3.1. MLT Improved the Gut Microbiota Population in SD Mice
3.2. MLT Improved Gut Microbiota Metabolite Disproportionality in SD Mice
3.3. FMT Promote Re-Establishment of the Intestinal Microecology in Mice
3.4. MLT Supplementation Improved Plasma MLT Levels and Colonic Butyrate Production in SD Mice
3.5. Effect of Butyrate on MLT Improved Colitis in SD Mice
3.6. Effect of MCT1/HDAC3/GSK-3β/HIF-1α/NF-κB Pathway on Butyrate Mediates MLT-Improved Colitis
4. Discussions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Group | MLT (pg/mL) | Butyrate (µg/mg) | Acetate (µg/mg) | Propiornate (µg/mg) | Colitis |
---|---|---|---|---|---|
CON | 24,086 ± 0.236 a | 3.252 ± 0.009 Aa | 1.000 ± 0.497 | 0.789 ± 0.557 | − |
SD | 20,431 ± 0.214 b | 2.587 ± 0.020 b | 1.146 ± 0.399 | 0.904 ± 0.447 | + |
ABs | 23,798 ± 0.223 a | 2.584 ± 0.004 b | 0.989 ± 0.204 | 0.781 ± 0.229 | + |
SD + ABs | 19,879 ± 0.593 b | 2.540 ± 0.013 b | 1.106 ± 0.267 | 0.873 ± 0.299 | + |
SD + MLT | 23,955 ± 0.431 a | 3.176 ± 0.019 a | 1.113 ± 0.131 | 0.878 ± 0.147 | − |
SD + Abs + MLT | 24,363 ± 0.398 a | 3.202 ± 0.018 Aa | 1.072 ± 0.049 | 0.846 ± 0.054 | − |
SD + Abs + Butyrate | 20,102 ± 0.225 b | 3.682 ± 0.033 Aa | 1.011 ± 0.094 | 0.797 ± 0.105 | − |
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Gao, T.; Wang, Z.; Dong, Y.; Cao, J.; Chen, Y. Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice. Int. J. Mol. Sci. 2021, 22, 11894. https://doi.org/10.3390/ijms222111894
Gao T, Wang Z, Dong Y, Cao J, Chen Y. Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice. International Journal of Molecular Sciences. 2021; 22(21):11894. https://doi.org/10.3390/ijms222111894
Chicago/Turabian StyleGao, Ting, Zixu Wang, Yulan Dong, Jing Cao, and Yaoxing Chen. 2021. "Melatonin-Mediated Colonic Microbiota Metabolite Butyrate Prevents Acute Sleep Deprivation-Induced Colitis in Mice" International Journal of Molecular Sciences 22, no. 21: 11894. https://doi.org/10.3390/ijms222111894