Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS)
Abstract
:1. Introduction
2. Methods
2.1. Colony Maintenance and Mice Monitoring
2.2. Tissue Preparation
2.3. RNA Isolation and RT-qPCR
2.4. Protein Extraction and Western Blot Analysis
2.5. Measurement of Metabolic Markers in Plasma
2.6. Statistical Analysis
3. Results
3.1. Leptin Levels Are Altered in WAT of TDP-43A315T Mice
3.2. Peripheral Levels of Leptin, Ghrelin, and Resistin Are Altered in Plasma of TDP-43A315T Mice
3.3. Hypothalamic Leptin Signaling in TDP-43A315T Mice
3.4. Leptin Signaling in the Spinal Cord of TDP-43A315T
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ferrer-Donato, A.; Contreras, A.; Frago, L.M.; Chowen, J.A.; Fernandez-Martos, C.M. Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS). Int. J. Mol. Sci. 2021, 22, 10305. https://doi.org/10.3390/ijms221910305
Ferrer-Donato A, Contreras A, Frago LM, Chowen JA, Fernandez-Martos CM. Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS). International Journal of Molecular Sciences. 2021; 22(19):10305. https://doi.org/10.3390/ijms221910305
Chicago/Turabian StyleFerrer-Donato, Agueda, Ana Contreras, Laura M. Frago, Julie A. Chowen, and Carmen M. Fernandez-Martos. 2021. "Alterations in Leptin Signaling in Amyotrophic Lateral Sclerosis (ALS)" International Journal of Molecular Sciences 22, no. 19: 10305. https://doi.org/10.3390/ijms221910305