ER-SURF: Riding the Endoplasmic Reticulum Surface to Mitochondria
Round 1
Reviewer 1 Report
This review presents previous work focusing on the endoplasmic reticulum surface in the biosynthesis of mitochondrial proteins, and the manuscript as submitted has been suitably improved. The manuscript submitted has been suitably improved and is considered to be publishable, as it will be of interest to readers on the state of research in the field.
Reviewer 2 Report
Koch, Schuldiner and Herrmann made a significant improvement in the revised manuscript ijms-1374780. As stated in my previous review, the authors very well bring up the concept of ER surface being deployed as a platform for providing assistance in translocation and targeting organellar proteins such as mitochondrial membrane proteins. The authors provide very good illustrations with very specific examples also discussing the relevance of ER-mitochondria contact sites, ERAD, MAD and membrane extractors.
- Please see if DAKAP1 should be written as dAKAP1.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
Round 1
Reviewer 1 Report
Mitochondrial proteins are targeted to the surface of the mitochondria after translation, which involves the surface of the endoplasmic reticulum. Although understanding this molecular mechanism is important for the quality control system of proteins in vivo, there are still many unanswered questions. This review introduces past research focusing on the endoplasmic reticulum surface in the biosynthesis of mitochondrial proteins. The content of the past research itself is organized in an easy-to-understand manner and is considered to be useful for readers to understand the status of research in this field. On the other hand, the description is written in a textbook-like style. It would be more useful for researchers who are actually working in this research field if there were specific descriptions of the experimental methods used to obtain the results of each fact in the past research.
Reviewer 2 Report
Thank you for asking me to review this manuscript. The review outlines the interactions between the endoplasmic reticulum, and mitochondria, demonstrating the complexity of the mechanisms for targetting and importing protein to the mitochondria.
The review is concise and well written, with helpful figures illustrating the concepts. References are appropriate, and current gaps in knowledge are highlighted.
I have no specific additional comments to suggest for this manuscript.
Reviewer 3 Report
Koch, Schuldiner and Herrmann did a remarkable job in writing the manuscript ijms-1327391. The authors very well bring up the concept of ER surface being deployed as a platform for providing assistance in translocation and targeting organellar proteins such as mitochondrial membrane proteins. The authors provide very good illustrations with very specific examples also discussing the relevance of ER-mitochondria contact sites, ERAD, MAD and membrane extractors.
While the authors provide specific examples in every section, I suggest the authors to also discuss at least one particular example in section 7. "ER-mitochondria contact zones as protein nurseries."
