Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens
Abstract
:1. Introduction
2. Results
2.1. Plasma IL-6, IL-1β Concentrations, and Cardiac Inflammation State and Chemotaxis
2.2. Cardiac Fibrosis
2.3. Endomplasmic Reticulum (ER) Stress and Autophagy
2.4. VDR and NFκB Activation
3. Discussion
4. Materials and Methods
4.1. Animal Management
4.2. Necropsy and Tissue Collection
4.3. Plasma IL-6 and IL-1β Concentrations and Cardiac Leukocyte Infiltration and Fibrosis
4.4. Chemoattraction Analysis
4.5. Western Blot Analysis
4.6. Gene Expression by qRT-PCR
4.7. Statistics
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Chou, P.-C.; Chen, Y.-H.; Chung, T.-K.; Walzem, R.L.; Lai, L.-S.; Chen, S.-E. Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens. Int. J. Mol. Sci. 2020, 21, 8379. https://doi.org/10.3390/ijms21218379
Chou P-C, Chen Y-H, Chung T-K, Walzem RL, Lai L-S, Chen S-E. Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens. International Journal of Molecular Sciences. 2020; 21(21):8379. https://doi.org/10.3390/ijms21218379
Chicago/Turabian StyleChou, Pao-Chia, Yu-Hui Chen, Thau-Kiong Chung, Rosemary L. Walzem, Lih-Shiuh Lai, and Shuen-Ei Chen. 2020. "Supplemental 25-hydroxycholecalciferol Alleviates Inflammation and Cardiac Fibrosis in Hens" International Journal of Molecular Sciences 21, no. 21: 8379. https://doi.org/10.3390/ijms21218379