Next Article in Journal
The Mitochondrial Proteomic Signatures of Human Skeletal Muscle Linked to Insulin Resistance
Next Article in Special Issue
Optimized Protocol for Isolation of Small Extracellular Vesicles from Human and Murine Lymphoid Tissues
Previous Article in Journal
Beneficial Effects of Mineralocorticoid Receptor Antagonism on Myocardial Fibrosis in an Experimental Model of the Myxomatous Degeneration of the Mitral Valve
Previous Article in Special Issue
Small Extracellular Vesicles Isolated from Serum May Serve as Signal-Enhancers for the Monitoring of CNS Tumors
 
 
Review
Peer-Review Record

Microvesicles in Cancer: Small Size, Large Potential

Int. J. Mol. Sci. 2020, 21(15), 5373; https://doi.org/10.3390/ijms21155373
by Kerstin Menck 1,†, Suganja Sivaloganathan 1,†, Annalen Bleckmann 1,2 and Claudia Binder 2,*
Reviewer 1: Anonymous
Reviewer 2: Anonymous
Int. J. Mol. Sci. 2020, 21(15), 5373; https://doi.org/10.3390/ijms21155373
Submission received: 30 June 2020 / Revised: 23 July 2020 / Accepted: 27 July 2020 / Published: 28 July 2020
(This article belongs to the Special Issue Extracellular Vesicles: Biology and Potentials in Cancer Therapeutics)

Round 1

Reviewer 1 Report

Authors reviewed the MV in cancer focusing on protein. Authors emphasized in the importance of the methods of isolation of MV. The review would be useful to understand the MV in malignancies.

Auhors mentioned the differences of MV by the methods of purification. Please summarize the several methods to isolate MV, such as ultracentrifuges, sucrose cushion ultracenrifuges, and precipitations by antibody.

Authors refer the position paper of ISEV (ref 7). Please summarize the appropriate methods for the isolation of EV and the necessary markers for the validation of the appropriate methods,

To measure the protein levels in MV, the SRM/MRM is also promising. Discuss about it.

Urinary MV is also good sources analyzing the MV from urological cancer. Discuss about them.

Please add the tumor antigens in urological cancer in Table 1.

Author Response

Please see cover letter attached

Author Response File: Author Response.pdf

Reviewer 2 Report

This review summarized function of microvesicles in the TME. It's nicely written with good drawings. It should be published.

Author Response

Please see cover letter attached

Author Response File: Author Response.pdf

Round 2

Reviewer 1 Report

Authors revised the manuscript adequately.

Back to TopTop