One New Phenolic Compound from Castanea mollissima Shells and its Suppression of HepatomaCell Proliferation and Inflammation by Inhibiting NF-κB Pathway
Abstract
:1. Introduction
2. Results
2.1. Chemistry
2.2. Antiproliferative Effect of These Compounds
2.3. Castanol B(1)-Induced Cellular Apoptosis
2.4. Effect of Castanol B (1) on LPS-Induced TNF-α and IL-6 Secretion in Hepatoma Cells
2.5. Effect Castanol B (1) on the TLR4–NF-κBSignal Pathway
3. Discussion
4. Materials and Methods
4.1. General
4.2. Plant Material
4.3. Water-Soluble Extract of CMS and Compound Isolation
4.4. Castanol B (1)
4.5. 7,8,dihydroxy-2H- chromen-3-one (2)
4.6. MTT Assay
4.7. Cell Apoptosis Assay
4.8. Cytokine Assays
4.9. Western Blotting
5. Statistical Analysis
6. Conclusions
Reference
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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No. | 1 | 2 |
---|---|---|
1 | 132.2 | |
2 | 118.6 | 73.6 |
3 | 116.9 | 206.7 |
4 | 113.9 | 34.8 |
5 | 157.5 | 125.8 |
6 | 116.9 | 111.2 |
7 | 169.9 | 154.6 |
8 | 148.0 | |
9 | 108.2 | |
10 | 148. | |
1’ | 145.1 | |
2’ | 113.5 | |
3’ | 145.2 | |
4’ | 142.6 | |
5’ | 115.3 | |
6’ | 118.0 |
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Wu, F.; Yao, X.; Xu, J.; Wu, Y.; Yang, Y.; Jin, Y.; Xie, H.; Liu, Y.; Yang, Y.; Zheng, X. One New Phenolic Compound from Castanea mollissima Shells and its Suppression of HepatomaCell Proliferation and Inflammation by Inhibiting NF-κB Pathway. Int. J. Mol. Sci. 2019, 20, 466. https://doi.org/10.3390/ijms20030466
Wu F, Yao X, Xu J, Wu Y, Yang Y, Jin Y, Xie H, Liu Y, Yang Y, Zheng X. One New Phenolic Compound from Castanea mollissima Shells and its Suppression of HepatomaCell Proliferation and Inflammation by Inhibiting NF-κB Pathway. International Journal of Molecular Sciences. 2019; 20(3):466. https://doi.org/10.3390/ijms20030466
Chicago/Turabian StyleWu, Fei, Xuan Yao, Jian Xu, Yue Wu, Yuejun Yang, Yu Jin, Huifang Xie, Yuancai Liu, Yifu Yang, and Xiangwei Zheng. 2019. "One New Phenolic Compound from Castanea mollissima Shells and its Suppression of HepatomaCell Proliferation and Inflammation by Inhibiting NF-κB Pathway" International Journal of Molecular Sciences 20, no. 3: 466. https://doi.org/10.3390/ijms20030466