Widely Targeted Metabolomics Analysis Reveals Great Changes in Nonvolatile Metabolites of Oolong Teas during Long-Term Storage
Abstract
:1. Introduction
2. Results and Discussion
2.1. Nonvolatile Metabolite Profile of Oolong Teas with Storage Age
2.2. Metabolite Changes in Paired Comparisons
2.3. Changes of Important Metabolites during Storage by K-Means Clustering Analysis
2.3.1. Flavanols and Tannins
2.3.2. Flavonols and Phenolic Acids
2.3.3. Amino Acids and Nucleotides
2.3.4. Alkaloids, Fatty Acids, Organic Acids, and Others
3. Materials and Methods
3.1. Experimental Materials
3.2. Sample Preparation and Extraction
3.3. UPLC Conditions
3.4. ESI-Q TRAP-MS/MS
3.5. Qualitative and Quantitative Analysis of Metabolites
3.6. Quality Control
3.7. Data Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Sample Availability
Abbreviations
References
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Hong, C.; Yue, W.; Shen, Q.; Wang, W.; Meng, H.; Guo, Y.; Xu, W.; Guo, Y. Widely Targeted Metabolomics Analysis Reveals Great Changes in Nonvolatile Metabolites of Oolong Teas during Long-Term Storage. Molecules 2021, 26, 7278. https://doi.org/10.3390/molecules26237278
Hong C, Yue W, Shen Q, Wang W, Meng H, Guo Y, Xu W, Guo Y. Widely Targeted Metabolomics Analysis Reveals Great Changes in Nonvolatile Metabolites of Oolong Teas during Long-Term Storage. Molecules. 2021; 26(23):7278. https://doi.org/10.3390/molecules26237278
Chicago/Turabian StyleHong, Cuiyun, Wenjie Yue, Qingyu Shen, Wenhua Wang, Hongyan Meng, Ying Guo, Wenjiang Xu, and Yaling Guo. 2021. "Widely Targeted Metabolomics Analysis Reveals Great Changes in Nonvolatile Metabolites of Oolong Teas during Long-Term Storage" Molecules 26, no. 23: 7278. https://doi.org/10.3390/molecules26237278