Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution
Abstract
:1. Approaches to Determine Protein–Protein Interaction Affinity (KD)
2. Theoretical Principles of Protein–Protein Interaction Affinity (KD) Determination from Acceptor Emission Using Quantitative FRET (qFRET) Analysis
3. Determination of Protein–Protein Interaction Affinity, KD, from FRET Donor Quenching
4. Comparison of Protein Interaction Affinity (KD) Determinations through FRET Acceptor Emission and FRET Donor Quenching Methods
5. Conclusions and Future Directions
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Liao, J.; Madahar, V.; Dang, R.; Jiang, L. Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution. Molecules 2021, 26, 6339. https://doi.org/10.3390/molecules26216339
Liao J, Madahar V, Dang R, Jiang L. Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution. Molecules. 2021; 26(21):6339. https://doi.org/10.3390/molecules26216339
Chicago/Turabian StyleLiao, Jiayu, Vipul Madahar, Runrui Dang, and Ling Jiang. 2021. "Quantitative FRET (qFRET) Technology for the Determination of Protein–Protein Interaction Affinity in Solution" Molecules 26, no. 21: 6339. https://doi.org/10.3390/molecules26216339