Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy
Abstract
:1. Introduction
2. Results
2.1. Bee Pollen Multi-Elemental Analysis
2.1.1. ED-XRF Quantitative Analysis of the Samples P1–P5 Using a Standard Sample
2.1.2. ED-XRF Qual-Quantitative Analysis of the Samples (P1–P5) without a Standard Sample
2.1.3. ED-XRF Quantitative Analysis (Using a Standard Sample) of Commercially Available Pollen Samples
2.1.4. Principal Component Analysis and Cluster Analysis
2.2. ATR-FTIR Spectroscopy
ATR–FTIR Spectroscopy Chemometric Analysis
3. Discussion
4. Materials and Methods
4.1. Samples
4.2. ICP-MS and ICP-OES Analysis
4.3. ED-XRF Analysis
4.4. ATR–FTIR Spectroscopy
4.5. Data Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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Mineral | Method | Sample (Date) | Mean + SD [mg/kg] | Method | Mean + SD [mg/kg] | Method | Mean [mg/kg] |
---|---|---|---|---|---|---|---|
Manganese | ED-XRF Qual-Quantitative analysis without standard sample | P2 (2 June 2019) | 37.0 ± 7.1 | ED-XRF Fundamental Parameter method with standard sample | 53.3 ± 11.8 | ICP-MS | 62.0 |
Copper | 8.1 ± 0.2 | 4.6 ± 0.2 | 5.7 | ||||
Zinc | 26.8 ± 4.9 | 26.6 ± 0.2 | 31.0 | ||||
Potassium | 4115.1 ± 263.9 | 4611.6 ± 379.6 | ICP-OES | 4600.0 | |||
Calcium | 1131.4 ± 133.3 | 1376.5 ± 143.8 | 1500.0 | ||||
Iron | 48.0 ± 5.4 | 63.9 ± 2.2 | 49.0 | ||||
Phosphorus | 3085.9 ± 69.7 | 4482.5 ± 490.5 | 4600.0 | ||||
Sulfur | 2397.8 ± 224.4 | 2547.7 ± 385.7 | 2700.0 | ||||
Chlorine | 375.1 ± 72.7 | - | - | ||||
Manganese | ED-XRF Qual-Quantitative analysis without standard sample | P5 (20 July 2019) | 15.7 ± 2.1 | ED-XRF Fundamental Parameter method with standard sample | 17.0 ± 0.7 | ICP-MS | 16.0 |
Copper | 8.6 ± 0.6 | 4.23 ± 0.4 | 4.4 | ||||
Zinc | 48.5 ± 0.4 | 39.0 ± 1.9 | 41.0 | ||||
Potassium | 4146.3 ± 27.3 | 4600.7 ± 71.6 | ICP-OES | 4400.0 | |||
Calcium | 608.3 ± 14.1 | 697.9 ± 24.1 | 680.0 | ||||
Iron | 63.2 ± 4.8 | 67.2 ± 8.2 | 68.0 | ||||
Phosphorus | 2551.1 ± 80.1 | 3475.5 ± 124.4 | 3500.0 | ||||
Sulfur | 1915.2 ± 16.6 | 1907.5 ± 44.3 | 1900.0 | ||||
Bromine | 19.1 ± 2.7 | - | - | ||||
Chlorine | 615.1 ± 19.4 | - | - |
Wavenumber (cm−1) | Vibrations and Biochemical Assignment |
---|---|
~1735 | C=O stretching mode from lipids and hemicellulose |
~1652 | C=O stretching mode from amide I of proteins |
~1546 | N–H deformation mode and C–N stretching mode from amide II (shoulder) |
~1514 | C=C stretching mode from phenolic acids |
~1420–1380 | C–H deformation mode from lipids and cellulose |
~1300–1200 | N–H deformation mode and C–N stretching mode from amide III of proteins |
~1180–950 | C–O and C–C stretching mode from sugar and proteins |
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Swiatly-Blaszkiewicz, A.; Pietkiewicz, D.; Matysiak, J.; Czech-Szczapa, B.; Cichocka, K.; Kupcewicz, B. Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy. Molecules 2021, 26, 6024. https://doi.org/10.3390/molecules26196024
Swiatly-Blaszkiewicz A, Pietkiewicz D, Matysiak J, Czech-Szczapa B, Cichocka K, Kupcewicz B. Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy. Molecules. 2021; 26(19):6024. https://doi.org/10.3390/molecules26196024
Chicago/Turabian StyleSwiatly-Blaszkiewicz, Agata, Dagmara Pietkiewicz, Jan Matysiak, Barbara Czech-Szczapa, Katarzyna Cichocka, and Bogumiła Kupcewicz. 2021. "Rapid and Accurate Approach for Honeybee Pollen Analysis Using ED-XRF and FTIR Spectroscopy" Molecules 26, no. 19: 6024. https://doi.org/10.3390/molecules26196024