Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay
Abstract
:1. Introduction
2. Results and Discussion
2.1. Principle of Analysis
2.2. Characterization of Tetracycline-BSA and Aptamer-AuNPs
2.3. Analytical Performance of the Present Method for Tetracycline Determination
2.4. Detection of Tetracycline in Real Samples
3. Materials and Method
3.1. Reagents and Instruments
3.2. Preparation of the Conjugate of Tetracycline with Bovine Serum Protein (Tetracycline-BSA)
3.3. Preparation of the Tetracycline-BSA-Coated Microplate
3.4. Preparation of Tetracycline Aptamer Labeled Gold Nanoparticles (Aptamer-AuNPs)
3.5. Determination of Tetracycline in Honey
3.6. Validation Procedure
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Spike (ng/mL) | Intra-Day Precision | Inter-Day Precision | ||
---|---|---|---|---|
Detected (ng/mL) | CV | Detected (ng/mL) | CV | |
0.1 | 0.10 ± 0.00 | 3.46% | 0.10 ± 0.01 | 7.17% |
1 | 0.97 ± 0.03 | 3.11% | 0.97 ± 0.05 | 5.20% |
5 | 4.76 ± 0.12 | 2.55% | 4.75 ± 0.20 | 4.25% |
Spike (ng/mL) | Detected (ng/mL) | Recovery | Mean Recovery | SD | CV | |
---|---|---|---|---|---|---|
1 | 0.1 | 0.0893 | 89.30% | 91.50% | 4.34% | 4.74% |
2 | 0.1 | 0.0965 | 96.50% | |||
3 | 0.1 | 0.0887 | 88.70% | |||
4 | 1 | 0.914 | 91.40% | 93.00% | 1.42% | 1.52% |
5 | 1 | 0.935 | 93.60% | |||
6 | 1 | 0.941 | 94.10% | |||
7 | 5 | 4.76 | 95.20% | 95.33% | 3.20% | 3.36% |
8 | 5 | 4.61 | 92.20% | |||
9 | 5 | 4.93 | 98.60% |
Method | Application | Apparatus | Range | LOD | Reference |
---|---|---|---|---|---|
SPR aptasensor containing oriented aptamer | honey | Biacore T200 SPR instrument | 0.01–1000 μg/kg | 0.0069 μg/kg | [20] |
Enzyme-linked aptamer assay with multivalent HRP-mimicking DNAzyme | honey | UV–vis spectrophotometer | 1.0 × 10−2 to 1.0 × 104 ng/mL | 8.1 × 10−2 ng/mL | [21] |
direct competitive assay-based aptasensor | honey | microplate reader | 0.1–1000 ng/mL | 0.0978 ng/mL | [22] |
Indirect competitive assay-based aptasensor | honey | microplate reader | 0.01–100 ng/mL | 9.6 × 10−3 ng/mL | [23] |
electrochemical aptasensor based on poly (L-glutamic acid)/MWCNTs modified glassy carbon electrode | honey | potentiostat/galvanostat | 1.0 × 10−16–1.0 × 10−6 M | 3.7 × 10−17 M | [24] |
Two aptasensors based on modified carbon paste/oleic acid and magnetic bar carbon paste/Fe3O4@oleic acid nanoparticle electrodes | drug, milk, honey and serum | potentiostat/galvanostat | 1.0 × 10−12–1.0 × 10−7 M; 1.0 × 10−10–1.0 × 10−7 M; | 3 × 10−13 M; 2.9 × 10−11 M; | [25] |
Graphene oxide-based aptasensor | honey | UV–vis spectrophotometer | 0.002–20 ng/mL | 0.001 ng/mL | [26] |
Colorimetric aptamer biosensor | milk | Microplate Spectrophotometer | 122 nM | [27] | |
Colorimetric aptasensor | milk | UV–vis spectrophotometer | 0.20–2.0 μg/mL | 0.039 μg/mL | [28] |
Electrochemical immunosensor | milk | electrochemical workstation | 0.08–1 ng/mL | 0.0321 ng/mL | [16] |
Near-infrared fluorescence-based multiplex lateral flow immunoassay | milk | odyssey infrared imaging system | 0.04–0.98 ng/mL | 0.04 ng/mL | [15] |
Immunochromatographic assay | serum | microplate reader | 0.7–26 ng/mL | 0.2 ng/mL | [14] |
Aptamer-based magnetic solid-phase extraction | water and honey | HPLC/UV | 10–3000 µg/L | 2.5 µg/L | [6] |
gold nanoparticle-linked aptamer assay | honey | microplate reader | 0.01–10 ng/mL | 0.0027 ng/mL | This work |
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Sheng, Y.-M.; Liang, J.; Xie, J. Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay. Molecules 2020, 25, 2144. https://doi.org/10.3390/molecules25092144
Sheng Y-M, Liang J, Xie J. Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay. Molecules. 2020; 25(9):2144. https://doi.org/10.3390/molecules25092144
Chicago/Turabian StyleSheng, Yan-Mei, Jian Liang, and Jing Xie. 2020. "Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay" Molecules 25, no. 9: 2144. https://doi.org/10.3390/molecules25092144