6-Methoxymellein Isolated from Carrot (Daucus carota L.) Targets Breast Cancer Stem Cells by Regulating NF-κB Signaling
Round 1
Reviewer 1 Report
The manuscript present the evaluation of the 6-Methoxymellein on the the proliferation, migration and mammosphere formation of breast cancer cells. Moreover the impact on the subpopulation of
CD44+/CD24- 336 and the expression of c-Myc, Sox2 and Oct4 proteins. In addition, the Authors demonstrated the effect on the NF-kBp65.
Comments:
The Authors should explain in Introduction or Discussion section using in the study these protein :-Myc, Oct4 and Sox2
The Authors should present the effect on the p50 subunits of NF-kB.
In my opinion it would be advisable designation expression of c-Myc, Oct4, Sox-2 and NF-kBp50 and p65 subunits
Author Response
We submit our reply to the review1 report.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
The manuscript by Liu et al., describes that 6-methoxymellein isolated from carrot (Daucus 2 carota L.) targets breast cancer stem cells by regulating NF-κB signaling. The specific concerns are as follows:
- Although the authors showed that 6-methoxymellein inhibited NF-κB nuclear shuttling, the ratio of increase of cytosolic fraction did not fit decrease of nuclear fraction.
- In Figure 6C, the data interpretation is conflicting.
- The authors did not show the direct evidence of NF-κB involvement in this regulation.
Author Response
We submit our reply to review 2 report.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
I accept in this form
Reviewer 2 Report
The authors have addressed the concerns I raised.
