Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L.
Abstract
:1. Introduction
2. Results
2.1. Larvicidal Activity of Helicteres velutina Fractions
2.2. Computational Study
2.3. Larvicidal Activity of the Promising Substances
3. Discussion
4. Materials and Methods
4.1. Collection, Extraction, and Compound Isolation
4.2. Virtual Screening Focused on Medicinal Chemistry
4.3. Molecular Docking
4.4. Larvicidal Activity
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of all isolated compounds are available from the authors. |
Training | Cross Validation | Test | |||||||
---|---|---|---|---|---|---|---|---|---|
Samples | Matches | % Matches | Samples | Matches | % Matches | Samples | Matches | % Matches | |
Active | 77 | 76 | 98% | 77 | 65 | 84% | 19 | 14 | 73% |
Inactive | 61 | 61 | 100% | 61 | 53 | 86% | 16 | 15 | 93% |
Total | 138 | 137 | 99% | 138 | 118 | 85% | 35 | 29 | 82% |
Protein ID | Classification | Ligand | Localisation |
---|---|---|---|
Aedes aegypti kynurenine aminotransferase (1YIY) | Transferase | 4′-Deoxy-4′-aminopyridoxal-5′-phosphate; Pyridoxamine-5′-phosphate | Adult mosquito head |
Sterol Carrier Protein-2 (1PZ4) | Lipid binding | Palmitic acid | Large intestine of larvae |
AaFKBP12 (3UQI) | Isomerase | 3 [N-morpholino]propane sulphonic acid | Adult mosquito |
Protein | Energy, PDB Value (kJ/mol) | Energy, MolDock Value (kJ/mol) | Redocking, RMSD Value |
---|---|---|---|
1YIY | −103.002 | −124.274 | 0.158 |
1PZ4 | −106.678 | −110.680 | 0.203 |
3UQI | −54.802 | −57.032 | 0.138 |
Molecule | Protein | p | ps | Pc |
---|---|---|---|---|
Tiliroside | 1YIY | 0.79 | 1 | 0.79 |
1PZ4 | 0.79 | 1 | 0.79 | |
3UQI | 0.79 | 1 | 0.79 | |
7,4′-di-O-methyl-8-O-sulphate flavone | 1YIY | 0.66 | 0.81 | 0.73 |
1PZ4 | 0.66 | 0.575 | 0.63 | |
3UQI | 0.66 | 0.45 | 0.59 | |
5,4′–di-hydroxy-7-methoxy-8-O-sulphate flavone (mariahine) | 1YIY | 0.64 | 0.83 | 0.60 |
1PZ4 | 0.64 | 0 | 0.41 | |
3UQI | 0.64 | 0.53 | 0.53 | |
5,3′–di-hydroxy-7,4′-dimethoxy-8-O-sulphate flavone (condadine) | 1YIY | 0.70 | 0.86 | 0.66 |
1PZ4 | 0.70 | 0 | 0.45 | |
3UQI | 0.70 | 0.38 | 0.54 | |
Sitosterol-3-O-β-d-glucopyranoside | 1YIY | 0.71 | 0.91 | 0.68 |
1PZ4 | 0.71 | 0.76 | 0.65 | |
3UQI | 10.71 | 0.64 | 0.62 |
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Fernandes, D.A.; Barros, R.P.C.; Teles, Y.C.F.; Oliveira, L.H.G.; Lima, J.B.; Scotti, M.T.; Nunes, F.C.; Conceição, A.S.; Vanderlei de Souza, M.d.F. Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L. Molecules 2019, 24, 2315. https://doi.org/10.3390/molecules24122315
Fernandes DA, Barros RPC, Teles YCF, Oliveira LHG, Lima JB, Scotti MT, Nunes FC, Conceição AS, Vanderlei de Souza MdF. Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L. Molecules. 2019; 24(12):2315. https://doi.org/10.3390/molecules24122315
Chicago/Turabian StyleFernandes, Diégina A., Renata P. C. Barros, Yanna C. F. Teles, Louise H. G. Oliveira, Jéssica B. Lima, Marcus T. Scotti, Fabíola C. Nunes, Adilva S. Conceição, and Maria de Fátima Vanderlei de Souza. 2019. "Larvicidal Compounds Extracted from Helicteres velutina K. Schum (Sterculiaceae) Evaluated against Aedes aegypti L." Molecules 24, no. 12: 2315. https://doi.org/10.3390/molecules24122315