Editor’s Choice Articles

Editor’s Choice articles are based on recommendations by the scientific editors of MDPI journals from around the world. Editors select a small number of articles recently published in the journal that they believe will be particularly interesting to readers, or important in the respective research area. The aim is to provide a snapshot of some of the most exciting work published in the various research areas of the journal.

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13 pages, 1124 KiB  
Article
Genotype × Environment Interaction and Stability Analysis of Selected Cassava Cultivars in South Africa
by Assefa B. Amelework, Michael W. Bairu, Roelene Marx, Mark Laing and Sonja L. Venter
Plants 2023, 12(13), 2490; https://doi.org/10.3390/plants12132490 - 29 Jun 2023
Cited by 5 | Viewed by 1416
Abstract
Cassava (Manihot esculenta Crantz) is an important root crop worldwide. It is adapted to a wide range of environmental conditions, exhibiting differential genotypic responses to varying environmental conditions. The objectives of this study were: (1) to examine the effect of genotype, environment [...] Read more.
Cassava (Manihot esculenta Crantz) is an important root crop worldwide. It is adapted to a wide range of environmental conditions, exhibiting differential genotypic responses to varying environmental conditions. The objectives of this study were: (1) to examine the effect of genotype, environment and genotype × environment interaction (GEI) on fresh root yield (FRY) and dry matter content (DMC); and (2) to identify superior genotypes that exhibit high performance for the traits of interest using the genetic tools of additive main effects and multiplicative interaction (AMMI) and genotype stability index (GSI) analysis. Eleven cassava genotypes were evaluated in a randomized complete block design at six trial sites in South Africa. The combined analysis of variance based on AMMI revealed significant genotype, environment and GEI for the traits. The percentage variation due to GEI was higher than the percentage variation due to genotype for FRY, reflecting differential genotypic responses across the experimental sites. The proportion of variance due to genotype variation was larger for DMC. Genotype stability index (GSI) showed that UKF3 (G6), 98/0002 (G2) and P4/10 (G5) were the highest yielding and most stable genotypes for FRY, and 98/0002 (G1), UKF3 (G6) and UKF9 (G11) were the highest yielding and most stable genotypes for DMC. Cultivars 98/0002 and UKF3 were identified as providing high stability with superior fresh root yield and DMC. These genotypes could be recommended to farmers for food, feed and industrial applications without the need for further breeding. The AMMI-2 model clustered the testing environments into three mega-environments based on the winning genotypes for FRY and DMC. Mabuyeni (KwaZulu-Natal), Shatale (Mpumalanga) and Mandlakazi (Limpopo) would be the best testing sites in future cassava-genotype evaluation and breeding programs. This study provides a baseline for a future study on the GEI of cassava varieties, using a larger set of genotypes, factoring in seasonal variation. Full article
(This article belongs to the Special Issue Plant Biotechnology and Crop Improvement)
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15 pages, 2366 KiB  
Article
GABA Application Enhances Drought Stress Tolerance in Wheat Seedlings (Triticum aestivum L.)
by Qiuyan Zhao, Yan Ma, Xianqing Huang, Lianjun Song, Ning Li, Mingwu Qiao, Tiange Li, Dan Hai and Yongxia Cheng
Plants 2023, 12(13), 2495; https://doi.org/10.3390/plants12132495 - 29 Jun 2023
Cited by 7 | Viewed by 1420
Abstract
In this study, the effects of γ-aminobutyric acid (GABA) on physio-biochemical metabolism, phenolic acid accumulation, and antioxidant system enhancement in germinated wheat under drought stress was investigated. The results showed that exogenous GABA reduced the oxidative damage in wheat seedlings caused by drought [...] Read more.
In this study, the effects of γ-aminobutyric acid (GABA) on physio-biochemical metabolism, phenolic acid accumulation, and antioxidant system enhancement in germinated wheat under drought stress was investigated. The results showed that exogenous GABA reduced the oxidative damage in wheat seedlings caused by drought stress and enhanced the content of phenolics, with 1.0 mM being the most effective concentration. Six phenolic acids were detected in bound form, including p-hydroxybenzoic acid, vanillic acid, syringic acid, p-coumaric acid, ferulic acid, and sinapic acid. However, only syringic acid and p-coumaric acid were found in free form. A total of 1.0 mM of GABA enhanced the content of total phenolic acids by 28% and 22%, respectively, compared with that of drought stress, on day four and day six of germination. The activities of phenylalanine ammonia lyase (PAL), cinnamic acid 4-hydroxylase (C4H) and 4-coumarate coenzyme A ligase (4CL) were activated by drought stress plus GABA treatment. Antioxidant enzyme activities were also induced. These results indicate that GABA treatment may be an effective way to relieve drought stress as it activates the antioxidant system of plants by inducing the accumulation of phenolics and the increase in antioxidant enzyme activity. Full article
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14 pages, 1874 KiB  
Article
Developing Functional Relationships between Soil Moisture Content and Corn Early-Season Physiology, Growth, and Development
by Ranadheer Reddy Vennam, Purushothaman Ramamoorthy, Sadikshya Poudel, Kambham Raja Reddy, William Brien Henry and Raju Bheemanahalli
Plants 2023, 12(13), 2471; https://doi.org/10.3390/plants12132471 - 28 Jun 2023
Cited by 8 | Viewed by 1633
Abstract
Drought is a severe threat to agriculture production that affects all growth stages of plants, including corn (Zea mays L.). Any factor affecting early seedling growth and development will significantly impact yield. Despite the recurrence of low rainfall during the growing seasons, [...] Read more.
Drought is a severe threat to agriculture production that affects all growth stages of plants, including corn (Zea mays L.). Any factor affecting early seedling growth and development will significantly impact yield. Despite the recurrence of low rainfall during the growing seasons, corn responses to different early-season soil moisture content levels have not been investigated. In this study, we investigated how corn morpho-physiological and biomass traits responded to varied soil moisture content during the early vegetative stage. Two corn hybrids were grown in a pot-culture facility under five different soil moisture treatments (0.15, 0.12, 0.09, 0.06, and 0.03 m3 m−3 volumetric water content, VWC) to assess the growth and developmental responses to varied soil moisture content during early-season growth (V2 to V7) stage. Sub-optimal soil moisture content limited plant growth and development by reducing physiological and phenotypic expression. Stomatal conductance and transpiration were decreased by an average of 65% and 59% across stress treatments relative to optimum conditions. On average, soil moisture deficit reduced the total leaf area by 71% and 72% compared to the control in ‘A6659VT2RIB’ and ‘P1316YHR’, respectively. Shoot and root dry weights were reduced by 74% and 43% under 0.03 m3 m−3 VWC. An increase in the root-to-shoot ratio was noticed under low VWC conditions compared to the control. Based on the stress tolerance index, the physiology and leaf growth parameters were more sensitive to soil moisture deficit. Our results highlight the impact of sub-optimal soil moisture on physiology and morphological traits during early-season growth. ‘P1316YHR’ demonstrated better physiological performance under stress conditions, while ‘A6659VT2RIB’ produced relatively better root growth. The findings suggest that biomass partitioning between shoot and root components is dynamic and depends on stress intensity. The current findings can help to prioritize traits associated with the early-season drought tolerance in corn. The functional relationships developed between soil moisture content and growth and developmental responses can be integrated into corn crop modeling to allow better irrigation management decisions. Full article
(This article belongs to the Special Issue Crops and Environmental Stresses: Phenomes to Genomes)
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11 pages, 1015 KiB  
Article
Effect of Amber (595 nm) Light Supplemented with Narrow Blue (430 nm) Light on Tomato Biomass
by Bo-Sen Wu, Mahnaz Mansoori, Keli Trumpler, Philip Wiredu Addo, Sarah MacPherson and Mark Lefsrud
Plants 2023, 12(13), 2457; https://doi.org/10.3390/plants12132457 - 27 Jun 2023
Cited by 7 | Viewed by 1730
Abstract
Full-spectrum light-emitting diodes (LEDs) mainly comprising 460-nm + 595-nm light are becoming a mainstay in the horticulture industry, and recent studies indicate that plant productivity under white LEDs is higher than combined blue and red LED lighting. Different light properties (wavelength and bandwidth) [...] Read more.
Full-spectrum light-emitting diodes (LEDs) mainly comprising 460-nm + 595-nm light are becoming a mainstay in the horticulture industry, and recent studies indicate that plant productivity under white LEDs is higher than combined blue and red LED lighting. Different light properties (wavelength and bandwidth) in full-spectrum light, particularly for the blue and amber light regions, have only partly been explored. This research aimed to characterize the effects of amber + blue light wavelengths and bandwidths on tomato (Solanum lycopersicum cv. Beefsteak) growth, morphology, and production efficiency. Tomato seedlings were subjected to four different light treatments for 60 days: narrow amber light (595 nm), narrow blue + narrow amber light (430 nm + 595 nm) with a 1:10 ratio, white LED (455 nm + 595 nm), and a high-pressure sodium (HPS) lamp (control). The highest mean fresh mass yield occurred with the narrow blue + narrow amber light (479 g), followed by white LED at 20% less, HPS at 34% less, and narrow amber at 40% less. Dry mass and plant height were similar among light treatments. Supplementing narrow amber light with 430-nm blue light led to a 20% increase in chlorophyll content. Findings indicate that narrow amber light is more efficient in biomass accumulation than broad amber light and that precise selection of different blue and amber wavelengths can greatly impact the growth and development of tomato seedlings. This energy-efficient narrow-wavelength combination shows improvement over white LED lighting for maximizing tomato growth. Full article
(This article belongs to the Special Issue The Effects of LED Light Spectra and Intensities on Plant Growth 2.0)
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14 pages, 18585 KiB  
Article
Glandular and Non-Glandular Trichomes from Phlomis herba-venti subsp. pungens Leaves: Light, Confocal, and Scanning Electron Microscopy and Histochemistry of the Secretory Products
by Irina Neta Gostin
Plants 2023, 12(13), 2423; https://doi.org/10.3390/plants12132423 - 23 Jun 2023
Cited by 6 | Viewed by 4126
Abstract
The purpose of this paper is to highlight the morphological peculiarities of glandular and non-glandular trichomes from leaves of Phlomis herba-venti subsp. Pungens using light, confocal, and scanning electron microscopy. Histochemistry techniques were used to analyze the localization of different chemical compounds in [...] Read more.
The purpose of this paper is to highlight the morphological peculiarities of glandular and non-glandular trichomes from leaves of Phlomis herba-venti subsp. Pungens using light, confocal, and scanning electron microscopy. Histochemistry techniques were used to analyze the localization of different chemical compounds in secretory trichomes. Two types of non-glandular trichomes were identified: unicellular and branched. They were found more frequently on the lower epidermis of leaves in different stages of ontogenetic development. Glandular trichomes were categorized as capitate (C1 and C2) with different stalk lengths and one–four secretory cells and dendroids (D) with one–four secretory cells. The histochemical analyses revealed distinct secretory products in terms of composition and distribution among the three types of glandular trichomes. The dendroid category of glandular trichomes is rarely found in plants and is not characteristic of the Lamiaceae species. They were described and characterized from a micromorphological and histochemical point of view for the first time in P. herba-venti. Full article
(This article belongs to the Special Issue Morphological Features and Phytochemical Properties of Herbs II)
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19 pages, 9535 KiB  
Article
Temporal Changes of Leaf Spectral Properties and Rapid Chlorophyll—A Fluorescence under Natural Cold Stress in Rice Seedlings
by Árpád Székely, Tímea Szalóki, Mihály Jancsó, János Pauk and Csaba Lantos
Plants 2023, 12(13), 2415; https://doi.org/10.3390/plants12132415 - 22 Jun 2023
Cited by 4 | Viewed by 1351
Abstract
Nowadays, hyperspectral remote sensing data are widely used in nutrient management, crop yield forecasting and stress monitoring. These data can be acquired with satellites, drones and handheld spectrometers. In this research, handheld spectrometer data were validated by chlorophyll-a fluorescence measurements under natural cold [...] Read more.
Nowadays, hyperspectral remote sensing data are widely used in nutrient management, crop yield forecasting and stress monitoring. These data can be acquired with satellites, drones and handheld spectrometers. In this research, handheld spectrometer data were validated by chlorophyll-a fluorescence measurements under natural cold stress. The performance of 16 rice cultivars with different origins and tolerances was monitored in the seedling stage. The studies were carried out under field conditions across two seasons to simulate different temperature regimes. Twenty-four spectral indices and eleven rapid chlorophyll-a fluorescence parameters were compared with albino plants. We identified which wavelengths are affected by low temperatures. Furthermore, the differences between genotypes were characterized by certain well-known and two newly developed (AAR and RAR) indices based on the spectral difference between the genotype and albino plant. The absorbance, reflectance and transmittance differences from the control are suitable for the discrimination of tolerant-sensitive varieties, especially based on their shape, peak and shifting distance. The following wavelengths are capable of determining the tolerant varieties, namely 548–553 nm, 667–670 nm, 687–688 nm and 800–950 nm in case of absorbance; above 700 nm for reflectance; and the whole spectrum (400–1100 nm) for transmittance. Full article
(This article belongs to the Special Issue Abiotic Stress Tolerance in Rice and Rice Breeding)
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18 pages, 5699 KiB  
Review
Bacillus cabrialesii: Five Years of Research on a Novel Species of Biological Control and Plant Growth-Promoting Bacteria
by Karem Ma. Figueroa-Brambila, Alina Escalante-Beltrán, Amelia Cristina Montoya-Martínez, Alondra María Díaz-Rodríguez, Naomi Dayanna López-Montoya, Fannie Isela Parra-Cota and Sergio de los Santos-Villalobos
Plants 2023, 12(13), 2419; https://doi.org/10.3390/plants12132419 - 22 Jun 2023
Cited by 5 | Viewed by 2147
Abstract
Bacillus cabrialesii is a novel bacterial species isolated from wheat (Triticum turgidum L. subsp. durum) plants in the Yaqui Valley, Mexico, by our research team. Over years of research studying this strain at the cutting-edge level, it has shown different mechanisms [...] Read more.
Bacillus cabrialesii is a novel bacterial species isolated from wheat (Triticum turgidum L. subsp. durum) plants in the Yaqui Valley, Mexico, by our research team. Over years of research studying this strain at the cutting-edge level, it has shown different mechanisms of action. B. cabrialesii is strongly reported as a plant-growth-promoting bacterium and a biological control agent on wheat crops. Knowing this, B. cabrialesii has been brought from lab to field as part of a bacterial consortium, not to mention that there are ongoing investigations into formulating a cost-effective bioinoculant to increase the yield and/or quality of wheat. Moreover, studies of this novel species as a biocontrol agent in other crops (pepper, tomato, cucumber, and potato) are being carried out, with preliminary results that make B. cabrialesii a promising biological control agent, inhibiting the growth of phytopathogens. However, research into this bacterium has not only been reported in our country; there are many studies around the world in which promising native Bacillus strains end up being identified as B. cabrialesii, which reaffirms the fact that this bacterial species can promote plant growth and combat phytopathogens, showing great agrobiotechnological potential. Full article
(This article belongs to the Special Issue Beneficial Microorganisms in Sustainable Agriculture)
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21 pages, 3799 KiB  
Article
Hydrogen Sulfide Alleviates Cadmium Stress by Enhancing Photosynthetic Efficiency and Regulating Sugar Metabolism in Wheat Seedlings
by Xiang Zheng, Bei Zhang, Ni Pan, Xue Cheng and Wei Lu
Plants 2023, 12(13), 2413; https://doi.org/10.3390/plants12132413 - 22 Jun 2023
Cited by 5 | Viewed by 1296
Abstract
Hydrogen sulfide (H2S) plays prominent multifunctional roles in the mediation of various physiological processes and stress responses to plants. In this study, hydroponic experiments were carried out to explore the effects of NaHS pretreatment on the growth of wheat (Triticum [...] Read more.
Hydrogen sulfide (H2S) plays prominent multifunctional roles in the mediation of various physiological processes and stress responses to plants. In this study, hydroponic experiments were carried out to explore the effects of NaHS pretreatment on the growth of wheat (Triticum aestivum L.) under 50 μM cadmium (Cd). Compared with Cd treatment alone, 50 μM NaHS pretreatment increased the plant height, soluble sugar content of shoots and roots, and dry weight of shoots and roots under Cd stress, while the Cd concentration of shoots and roots was significantly reduced by 18.1% and 25.9%, respectively. Meanwhile, NaHS pretreatment protected the photosynthetic apparatus by increasing the net photosynthetic rate and PSII electron transportation rate of wheat leaves under Cd stress. NaHS pretreatment significantly increased the soluble sugar content to maintain the osmotic pressure balance of the leaf cells. The gene expression results associated with photosynthetic carbon assimilation and sucrose synthesis in wheat leaves suggested that the NaHS pretreatment significantly up-regulated the expression of TaRBCL, TaRBCS, and TaPRK, while it down-regulated the expression of TaFBA, TaSuSy, TaSAInv, and TaA/NInv. In summary, NaHS pretreatment improved the resistance of wheat seedlings under Cd stress by increasing the rate of photosynthesis and regulating the expression of genes related to sugar metabolism. Full article
(This article belongs to the Special Issue Abiotic Stresses in Cereals)
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29 pages, 2471 KiB  
Review
Fruit Photosynthesis: More to Know about Where, How and Why
by Andreia Garrido, Artur Conde, João Serôdio, Ric C. H. De Vos and Ana Cunha
Plants 2023, 12(13), 2393; https://doi.org/10.3390/plants12132393 - 21 Jun 2023
Cited by 6 | Viewed by 6387
Abstract
Not only leaves but also other plant organs and structures typically considered as carbon sinks, including stems, roots, flowers, fruits and seeds, may exhibit photosynthetic activity. There is still a lack of a coherent and systematized body of knowledge and consensus on the [...] Read more.
Not only leaves but also other plant organs and structures typically considered as carbon sinks, including stems, roots, flowers, fruits and seeds, may exhibit photosynthetic activity. There is still a lack of a coherent and systematized body of knowledge and consensus on the role(s) of photosynthesis in these “sink” organs. With regard to fruits, their actual photosynthetic activity is influenced by a range of properties, including fruit anatomy, histology, physiology, development and the surrounding microclimate. At early stages of development fruits generally contain high levels of chlorophylls, a high density of functional stomata and thin cuticles. While some plant species retain functional chloroplasts in their fruits upon subsequent development or ripening, most species undergo a disintegration of the fruit chloroplast grana and reduction in stomata functionality, thus limiting gas exchange. In addition, the increase in fruit volume hinders light penetration and access to CO2, also reducing photosynthetic activity. This review aimed to compile information on aspects related to fruit photosynthesis, from fruit characteristics to ecological drivers, and to address the following challenging biological questions: why does a fruit show photosynthetic activity and what could be its functions? Overall, there is a body of evidence to support the hypothesis that photosynthesis in fruits is key to locally providing: ATP and NADPH, which are both fundamental for several demanding biosynthetic pathways (e.g., synthesis of fatty acids); O2, to prevent hypoxia in its inner tissues including seeds; and carbon skeletons, which can fuel the biosynthesis of primary and secondary metabolites important for the growth of fruits and for spreading, survival and germination of their seed (e.g., sugars, flavonoids, tannins, lipids). At the same time, both primary and secondary metabolites present in fruits and seeds are key to human life, for instance as sources for nutrition, bioactives, oils and other economically important compounds or components. Understanding the functions of photosynthesis in fruits is pivotal to crop management, providing a rationale for manipulating microenvironmental conditions and the expression of key photosynthetic genes, which may help growers or breeders to optimize development, composition, yield or other economically important fruit quality aspects. Full article
(This article belongs to the Special Issue Photosynthetic Activity of Non-Primary Photosynthetic Organs)
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16 pages, 2036 KiB  
Article
Sage, Rosemary, and Bay Laurel Hydrodistillation By-Products as a Source of Bioactive Compounds
by Anđela Miljanović, Maja Dent, Dorotea Grbin, Sandra Pedisić, Zoran Zorić, Zvonimir Marijanović, Igor Jerković and Ana Bielen
Plants 2023, 12(13), 2394; https://doi.org/10.3390/plants12132394 - 21 Jun 2023
Cited by 9 | Viewed by 1966
Abstract
Essential oils from Mediterranean wild plants are widely used, but the hydrodistillation residues produced in parallel with these essential oils are significantly understudied and underexploited. Since there are only fragmentary data in the literature, we have, for the first time, systematically analyzed the [...] Read more.
Essential oils from Mediterranean wild plants are widely used, but the hydrodistillation residues produced in parallel with these essential oils are significantly understudied and underexploited. Since there are only fragmentary data in the literature, we have, for the first time, systematically analyzed the chemical composition of the by-products obtained after hydrodistillation of sage, bay laurel, and rosemary leaves, i.e., hydrolates, water residues, and solid residues. The chemical composition of the hydrolates changed compared to their respective essential oils towards the dominance of more hydrophilic, oxygenated compounds, such as camphor in sage, 1,8-cineole in bay laurel, and berbenone in rosemary. However, some compounds, mostly sesquiterpenes, which were present in considerable amounts in essential oils, were absent or only present in very small amounts in the hydrolates. Furthermore, both the water and the solid residues were rich in polyphenols, such as procyanidins in bay laurel and rosmarinic acid in rosemary and sage. In conclusion, we demonstrate the valuable chemical composition of sage, rosemary, and bay laurel hydrodistillation by-products and discuss a wide range of their possible applications. Full article
(This article belongs to the Topic Plants Volatile Compounds)
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14 pages, 4295 KiB  
Article
Evaluating Leaf Wettability and Salt Hygroscopicity as Drivers for Foliar Absorption
by Neriman Tuba Barlas, Héctor Alejandro Bahamonde, Carlos Pimentel, Pedro Domínguez-Huidobro, Carlos M. Pina and Victoria Fernández
Plants 2023, 12(12), 2357; https://doi.org/10.3390/plants12122357 - 18 Jun 2023
Cited by 7 | Viewed by 1639
Abstract
The objective of this study was to evaluate the rate of foliar absorption of magnesium (Mg) salts with different deliquescence and efflorescence relative humidity values (DRH and ERH, also known as point of deliquescence (POD) and point of efflorescence (POE), respectively) when supplied [...] Read more.
The objective of this study was to evaluate the rate of foliar absorption of magnesium (Mg) salts with different deliquescence and efflorescence relative humidity values (DRH and ERH, also known as point of deliquescence (POD) and point of efflorescence (POE), respectively) when supplied to leaves of model plants with different wettability properties. For this purpose, a greenhouse pot experiment was conducted with lettuce (very wettable), broccoli (highly unwettable) and leek (highly unwettable). Foliar sprays contained 0.1% surfactant plus 100 mM Mg supplied as MgCl2·6H2O, Mg(NO3)2·6H2O or MgSO4·7H2O. Leaf Mg concentrations were determined 1 and 7 days after foliar application. Anion concentrations were also measured in lettuce where a significant foliar Mg absorption was detected. Leaf wettability, leaf surface free energy and fertilizer drop deposit appearance onto the foliage were assessed. It is concluded that despite including a surfactant in the spray formulation, leaf wettability plays a major role in foliar Mg absorption. Full article
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15 pages, 2310 KiB  
Article
Fulvic Acid Improves Salinity Tolerance of Rice Seedlings: Evidence from Phenotypic Performance, Relevant Phenolic Acids, and Momilactones
by Akter Jesmin, La Hoang Anh, Nguyen Phuong Mai, Tran Dang Khanh and Tran Dang Xuan
Plants 2023, 12(12), 2359; https://doi.org/10.3390/plants12122359 - 18 Jun 2023
Cited by 4 | Viewed by 1755
Abstract
Salinity is a severe stress that causes serious losses in rice production worldwide. This study, for the first time, investigated the effects of fulvic acid (FA) with various concentrations of 0.125, 0.25, 0.5, and 1.0 mL/L on the ability of three rice varieties, [...] Read more.
Salinity is a severe stress that causes serious losses in rice production worldwide. This study, for the first time, investigated the effects of fulvic acid (FA) with various concentrations of 0.125, 0.25, 0.5, and 1.0 mL/L on the ability of three rice varieties, Koshihikari, Nipponbare, and Akitakomachi, to cope with a 10 dS/m salinity level. The results show that the T3 treatment (0.25 mL/L FA) is the most effective in stimulating the salinity tolerance of all three varieties by enhancing their growth performance. T3 also promotes phenolic accumulation in all three varieties. In particular, salicylic acid, a well-known salt-stress-resistant substance, is found to increase during salinity stress in Nipponbare and Akitakomachi treated with T3 by 88% and 60%, respectively, compared to crops receiving salinity treatment alone. Noticeably, the levels of momilactones A (MA) and B (MB) fall in salt-affected rice. However, their levels markedly rise in rice treated with T3 (by 50.49% and 32.20%, respectively, in Nipponbare, and by 67.76% and 47.27%, respectively, in Akitakomachi), compared to crops receiving salinity treatment alone. This implies that momilactone levels are proportional to rice tolerance against salinity. Our findings suggest that FA (0.25 mL/L) can effectively improve the salinity tolerance of rice seedlings even in the presence of a strong salt stress of 10 dS/m. Further studies on FA application in salt-affected rice fields should be conducted to confirm its practical implications. Full article
(This article belongs to the Special Issue Crop Plants Response to Abiotic Stresses)
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15 pages, 4046 KiB  
Article
Selecting High-Performing and Stable Pea Genotypes in Multi-Environmental Trial (MET): Applying AMMI, GGE-Biplot, and BLUP Procedures
by Sintayehu D. Daba, Alecia M. Kiszonas and Rebecca J. McGee
Plants 2023, 12(12), 2343; https://doi.org/10.3390/plants12122343 - 16 Jun 2023
Cited by 6 | Viewed by 1385
Abstract
A large amount of data on various traits is accumulated over the course of a breeding program and can be used to optimize various aspects of the crop improvement pipeline. We leveraged data from advanced yield trials (AYT) of three classes of peas [...] Read more.
A large amount of data on various traits is accumulated over the course of a breeding program and can be used to optimize various aspects of the crop improvement pipeline. We leveraged data from advanced yield trials (AYT) of three classes of peas (green, yellow, and winter peas) collected over ten years (2012–2021) to analyze and test key aspects fundamental to pea breeding. Six balanced datasets were used to test the predictive success of the BLUP and AMMI family models. Predictive assessment using cross-validation indicated that BLUP offered better predictive accuracy as compared to any AMMI family model. However, BLUP may not always identify the best genotype that performs well across environments. AMMI and GGE, two statistical tools used to exploit GE, could fill this gap and aid in understanding how genotypes perform across environments. AMMI’s yield by environmental IPCA1, WAASB by yield plot, and GGE biplot were shown to be useful in identifying genotypes for specific or broad adaptability. When compared to the most favorable environment, we observed a yield reduction of 80–87% in the most unfavorable environment. The seed yield variability across environments was caused in part by weather variability. Hotter conditions in June and July as well as low precipitation in May and June affected seed yield negatively. In conclusion, the findings of this study are useful to breeders in the variety selection process and growers in pea production. Full article
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21 pages, 5542 KiB  
Article
Genome-Wide Association Study of Seed Morphology Traits in Senegalese Sorghum Cultivars
by Ezekiel Ahn, Jacob Botkin, Vishnutej Ellur, Yoonjung Lee, Kabita Poudel, Louis K. Prom and Clint Magill
Plants 2023, 12(12), 2344; https://doi.org/10.3390/plants12122344 - 16 Jun 2023
Cited by 6 | Viewed by 1633
Abstract
Sorghum is considered the fifth most important crop in the world. Despite the potential value of Senegalese germplasm for various traits, such as resistance to fungal diseases, there is limited information on the study of sorghum seed morphology. In this study, 162 Senegalese [...] Read more.
Sorghum is considered the fifth most important crop in the world. Despite the potential value of Senegalese germplasm for various traits, such as resistance to fungal diseases, there is limited information on the study of sorghum seed morphology. In this study, 162 Senegalese germplasms were evaluated for seed area size, length, width, length-to-width ratio, perimeter, circularity, the distance between the intersection of length & width (IS) and center of gravity (CG), and seed darkness and brightness by scanning and analyzing morphology-related traits with SmartGrain software at the USDA-ARS Plant Science Research Unit. Correlations between seed morphology-related traits and traits associated with anthracnose and head smut resistance were analyzed. Lastly, genome-wide association studies were performed on phenotypic data collected from over 16,000 seeds and 193,727 publicly available single nucleotide polymorphisms (SNPs). Several significant SNPs were found and mapped to the reference sorghum genome to uncover multiple candidate genes potentially associated with seed morphology. The results indicate clear correlations among seed morphology-related traits and potential associations between seed morphology and the defense response of sorghum. GWAS analysis listed candidate genes associated with seed morphologies that can be used for sorghum breeding in the future. Full article
(This article belongs to the Special Issue Plant Reproduction and Embryonic Development)
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19 pages, 3816 KiB  
Article
Reflectance Spectroscopy for the Classification and Prediction of Pigments in Agronomic Crops
by Renan Falcioni, Werner Camargos Antunes, José Alexandre M. Demattê and Marcos Rafael Nanni
Plants 2023, 12(12), 2347; https://doi.org/10.3390/plants12122347 - 16 Jun 2023
Cited by 5 | Viewed by 1478
Abstract
Reflectance spectroscopy, in combination with machine learning and artificial intelligence algorithms, is an effective method for classifying and predicting pigments and phenotyping in agronomic crops. This study aims to use hyperspectral data to develop a robust and precise method for the simultaneous evaluation [...] Read more.
Reflectance spectroscopy, in combination with machine learning and artificial intelligence algorithms, is an effective method for classifying and predicting pigments and phenotyping in agronomic crops. This study aims to use hyperspectral data to develop a robust and precise method for the simultaneous evaluation of pigments, such as chlorophylls, carotenoids, anthocyanins, and flavonoids, in six agronomic crops: corn, sugarcane, coffee, canola, wheat, and tobacco. Our results demonstrate high classification accuracy and precision, with principal component analyses (PCAs)-linked clustering and a kappa coefficient analysis yielding results ranging from 92 to 100% in the ultraviolet–visible (UV–VIS) to near-infrared (NIR) to shortwave infrared (SWIR) bands. Predictive models based on partial least squares regression (PLSR) achieved R2 values ranging from 0.77 to 0.89 and ratio of performance to deviation (RPD) values over 2.1 for each pigment in C3 and C4 plants. The integration of pigment phenotyping methods with fifteen vegetation indices further improved accuracy, achieving values ranging from 60 to 100% across different full or range wavelength bands. The most responsive wavelengths were selected based on a cluster heatmap, β-loadings, weighted coefficients, and hyperspectral vegetation index (HVI) algorithms, thereby reinforcing the effectiveness of the generated models. Consequently, hyperspectral reflectance can serve as a rapid, precise, and accurate tool for evaluating agronomic crops, offering a promising alternative for monitoring and classification in integrated farming systems and traditional field production. It provides a non-destructive technique for the simultaneous evaluation of pigments in the most important agronomic plants. Full article
(This article belongs to the Special Issue Remote and Proximal Sensing for Plant Research)
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14 pages, 2232 KiB  
Article
Effects of Drought Stress Induced by Hypertonic Polyethylene Glycol (PEG-6000) on Passiflora edulis Sims Physiological Properties
by Ying Qi, Lingling Ma, Muhammad Imran Ghani, Qiang Peng, Ruidong Fan, Xiaojing Hu and Xiaoyulong Chen
Plants 2023, 12(12), 2296; https://doi.org/10.3390/plants12122296 - 12 Jun 2023
Cited by 5 | Viewed by 2628
Abstract
Passion fruit is known to be sensitive to drought, and in order to study the physiological and biochemical changes that occur in passion fruit seedlings under drought stress, a hypertonic polyethylene glycol (PEG) solution (5%, 10%, 15%, and 20%) was used to simulate [...] Read more.
Passion fruit is known to be sensitive to drought, and in order to study the physiological and biochemical changes that occur in passion fruit seedlings under drought stress, a hypertonic polyethylene glycol (PEG) solution (5%, 10%, 15%, and 20%) was used to simulate drought stress in passion fruit seedlings. We explored the physiological changes in passion fruit seedlings under drought stress induced by PEG to elucidate their response to drought stress and provide a theoretical basis for drought-resistant cultivation of passion fruit seedlings. The results show that drought stress induced by PEG had a significant effect on the growth and physiological indices of passion fruit. Drought stress significantly decreased fresh weight, chlorophyll content, and root vitality. Conversely, the contents of soluble protein (SP), proline (Pro), and malondialdehyde (MDA) increased gradually with the increasing PEG concentration and prolonged stress duration. After nine days, the SP, Pro and MDA contents were higher in passion fruit leaves and roots under 20% PEG treatments compared with the control. Additionally, with the increase in drought time, the activities of antioxidant enzymes such as peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) showed an increasing trend and then a decreasing trend, and they reached the highest value at the sixth day of drought stress. After rehydration, SP, Pro and MDA contents in the leaves and roots of passion fruit seedlings was reduced. Among all the stress treatments, 20% PEG had the most significant effect on passion fruit seedlings. Therefore, our study demonstrated sensitive concentrations of PEG to simulate drought stress on passion fruit and revealed the physiological adaptability of passion fruit to drought stress. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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19 pages, 337 KiB  
Review
Seaweeds in Food: Current Trends
by Cristian Rogel-Castillo, Monica Latorre-Castañeda, Camila Muñoz-Muñoz and Cristian Agurto-Muñoz
Plants 2023, 12(12), 2287; https://doi.org/10.3390/plants12122287 - 12 Jun 2023
Cited by 9 | Viewed by 2046
Abstract
Edible seaweeds are an excellent source of macronutrients, micronutrients, and bioactive compounds, and they can be consumed raw or used as ingredients in food products. However, seaweeds may also bioaccumulate potentially hazardous compounds for human health and animals, namely, heavy metals. Hence, the [...] Read more.
Edible seaweeds are an excellent source of macronutrients, micronutrients, and bioactive compounds, and they can be consumed raw or used as ingredients in food products. However, seaweeds may also bioaccumulate potentially hazardous compounds for human health and animals, namely, heavy metals. Hence, the purpose of this review is to analyze the recent trends of edible seaweeds research: (i) nutritional composition and bioactive compounds, (ii) the use and acceptability of seaweeds in foodstuffs, (iii) the bioaccumulation of heavy metals and microbial pathogens, and (iv) current trends in Chile for using seaweeds in food. In summary, while it is evident that seaweeds are consumed widely worldwide, more research is needed to characterize new types of edible seaweeds as well as their use as ingredients in the development of new food products. Additionally, more research is needed to maintain control of the presence of heavy metals to assure a safe product for consumers. Finally, the need to keep promoting the benefits of seaweed consumption is emphasized, adding value in the algae-based production chain, and promoting a social algal culture. Full article
(This article belongs to the Special Issue Seaweed Biology: Focusing on Food, Materials and Bioenergy)
22 pages, 434 KiB  
Review
Plants’ Response Mechanisms to Salinity Stress
by Thuvaraki Balasubramaniam, Guoxin Shen, Nardana Esmaeili and Hong Zhang
Plants 2023, 12(12), 2253; https://doi.org/10.3390/plants12122253 - 8 Jun 2023
Cited by 67 | Viewed by 11211
Abstract
Soil salinization is a severe abiotic stress that negatively affects plant growth and development, leading to physiological abnormalities and ultimately threatening global food security. The condition arises from excessive salt accumulation in the soil, primarily due to anthropogenic activities such as irrigation, improper [...] Read more.
Soil salinization is a severe abiotic stress that negatively affects plant growth and development, leading to physiological abnormalities and ultimately threatening global food security. The condition arises from excessive salt accumulation in the soil, primarily due to anthropogenic activities such as irrigation, improper land uses, and overfertilization. The presence of Na⁺, Cl, and other related ions in the soil above normal levels can disrupt plant cellular functions and lead to alterations in essential metabolic processes such as seed germination and photosynthesis, causing severe damage to plant tissues and even plant death in the worst circumstances. To counteract the effects of salt stress, plants have developed various mechanisms, including modulating ion homeostasis, ion compartmentalization and export, and the biosynthesis of osmoprotectants. Recent advances in genomic and proteomic technologies have enabled the identification of genes and proteins involved in plant salt-tolerance mechanisms. This review provides a short overview of the impact of salinity stress on plants and the underlying mechanisms of salt-stress tolerance, particularly the functions of salt-stress-responsive genes associated with these mechanisms. This review aims at summarizing recent advances in our understanding of salt-stress tolerance mechanisms, providing the key background knowledge for improving crops’ salt tolerance, which could contribute to the yield and quality enhancement in major crops grown under saline conditions or in arid and semiarid regions of the world. Full article
(This article belongs to the Special Issue Adaptive Mechanisms of Plants to Biotic or Abiotic Stresses)
20 pages, 2677 KiB  
Article
Hormetic Effect of Glyphosate on the Morphology, Physiology and Metabolism of Coffee Plants
by Renato Nunes Costa, Natalia da Cunha Bevilaqua, Fábio Henrique Krenchinski, Bruno Flaibam Giovanelli, Vinicius Gabriel Caneppele Pereira, Edivaldo Domingues Velini and Caio Antonio Carbonari
Plants 2023, 12(12), 2249; https://doi.org/10.3390/plants12122249 - 8 Jun 2023
Cited by 5 | Viewed by 1403
Abstract
Glyphosate is a nonselective herbicide of systemic action that inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase, thus compromising amino acid production and consequently the growth and development of susceptible plants. The objective of this study was to evaluate the hormetic effect of glyphosate on the [...] Read more.
Glyphosate is a nonselective herbicide of systemic action that inhibits the enzyme 5-enolpyruvylshikimate-3-phosphate synthase, thus compromising amino acid production and consequently the growth and development of susceptible plants. The objective of this study was to evaluate the hormetic effect of glyphosate on the morphology, physiology, and biochemistry of coffee plants. Coffee seedlings (Coffea arabica cv Catuaí Vermelho IAC-144) were transplanted into pots filled with a mixture of soil and substrate and subjected to ten doses of glyphosate: 0, 11.25, 22.5, 45, 90, 180, 360, 720, 1440, and 2880 g acid equivalent (ae) ha−1. Evaluations were performed using the morphological, physiological, and biochemical variables. Data analysis for the confirmation of hormesis occurred with the application of mathematical models. The hormetic effect of glyphosate on coffee plant morphology was determined by the variables plant height, number of leaves, leaf area, and leaf, stem, and total dry mass. Doses from 14.5 to 30 g ae ha−1 caused the highest stimulation. In the physiological analyses, the highest stimulation was observed upon CO2 assimilation, transpiration, stomatal conductance, carboxylation efficiency, intrinsic water use efficiency, electron transport rate, and photochemical efficiency of photosystem II at doses ranging from 4.4 to 55 g ae ha−1. The biochemical analyses revealed significant increases in the concentrations of quinic acid, salicylic acid, caffeic acid, and coumaric acid, with maximum stimulation at doses between 3 and 140 g ae ha−1. Thus, the application of low doses of glyphosate has positive effects on the morphology, physiology, and biochemistry of coffee plants. Full article
(This article belongs to the Special Issue Plant Biostimulation)
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15 pages, 3720 KiB  
Article
Successful In Vitro Shoot Multiplication of Quercus robur L. Trees Aged up to 800 Years
by Paweł Chmielarz, Szymon Kotlarski, Ewa Marzena Kalemba, João Paulo Rodrigues Martins and Marcin Michalak
Plants 2023, 12(12), 2230; https://doi.org/10.3390/plants12122230 - 6 Jun 2023
Cited by 5 | Viewed by 2035
Abstract
The conservation of the genetic resources of old trees is crucial to their ecological role but is extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed to assess the regenerative potential of [...] Read more.
The conservation of the genetic resources of old trees is crucial to their ecological role but is extremely difficult, especially for oak species (Quercus spp.) displaying recalcitrance in seed and vegetative propagation methods. Our study aimed to assess the regenerative potential of Quercus robur trees of different ages (up to 800 years) during micropropagation. We also aimed to determine how in vitro conditions can influence in vitro regeneration responses. Lignified branches collected from 67 selected trees were cultivated ex vitro in culture pots at 25 °C to obtain epicormic shoots (explant sources). The explants were cultivated on an agar medium supplemented with 0.8 mg L−1 6-benzylaminopurine (BAP) for at least 21 months. In a second experiment, two different shoot multiplication conditions (temporary immersion—RITA® bioreactor and agar medium) and two culture medium formulations (Woody Plant Medium and modified Quoirin and Lepoivre medium) were tested. The results showed that the mean length of the epicormic shoots obtained in a pot culture was a function of donor age and was similar among the group of younger trees (ca. 20–200 years), and varied between older trees (ca. 300–800 years). The efficiency of in vitro shoot multiplication strictly depended on the genotype. A sustainable in vitro culture (defined as survival after 6 months) was only possible for half of the tested old donor trees, even when they survived the first month of in vitro growth. A continuous monthly increase in the number of in vitro cultured shoots was reported in younger oaks and in some old oaks. We found a significant effect of the culture system and the macro- and micronutrient composition on in vitro shoot growth. This is the first report demonstrating that the in vitro culture can be successfully applied to the propagation of even 800-year-old pedunculate oak trees. Full article
(This article belongs to the Special Issue Application of Biotechnology to Woody Propagation)
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28 pages, 6036 KiB  
Article
An Accurate Classification of Rice Diseases Based on ICAI-V4
by Nanxin Zeng, Gufeng Gong, Guoxiong Zhou and Can Hu
Plants 2023, 12(11), 2225; https://doi.org/10.3390/plants12112225 - 5 Jun 2023
Cited by 11 | Viewed by 2487
Abstract
Rice is a crucial food crop, but it is frequently affected by diseases during its growth process. Some of the most common diseases include rice blast, flax leaf spot, and bacterial blight. These diseases are widespread, highly infectious, and cause significant damage, posing [...] Read more.
Rice is a crucial food crop, but it is frequently affected by diseases during its growth process. Some of the most common diseases include rice blast, flax leaf spot, and bacterial blight. These diseases are widespread, highly infectious, and cause significant damage, posing a major challenge to agricultural development. The main problems in rice disease classification are as follows: (1) The images of rice diseases that were collected contain noise and blurred edges, which can hinder the network’s ability to accurately extract features of the diseases. (2) The classification of disease images is a challenging task due to the high intra-class diversity and inter-class similarity of rice leaf diseases. This paper proposes the Candy algorithm, an image enhancement technique that utilizes improved Canny operator filtering (the gravitational edge detection algorithm) to emphasize the edge features of rice images and minimize the noise present in the images. Additionally, a new neural network (ICAI-V4) is designed based on the Inception-V4 backbone structure, with a coordinate attention mechanism added to enhance feature capture and overall model performance. The INCV backbone structure incorporates Inception-iv and Reduction-iv structures, with the addition of involution to enhance the network’s feature extraction capabilities from a channel perspective. This enables the network to better classify similar images of rice diseases. To address the issue of neuron death caused by the ReLU activation function and improve model robustness, Leaky ReLU is utilized. Our experiments, conducted using the 10-fold cross-validation method and 10,241 images, show that ICAI-V4 has an average classification accuracy of 95.57%. These results indicate the method’s strong performance and feasibility for rice disease classification in real-life scenarios. Full article
(This article belongs to the Collection Application of AI in Plants)
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26 pages, 1216 KiB  
Review
The Contribution of PGPR in Salt Stress Tolerance in Crops: Unravelling the Molecular Mechanisms of Cross-Talk between Plant and Bacteria
by Gianluigi Giannelli, Silvia Potestio and Giovanna Visioli
Plants 2023, 12(11), 2197; https://doi.org/10.3390/plants12112197 - 1 Jun 2023
Cited by 16 | Viewed by 4012
Abstract
Soil salinity is a major abiotic stress in global agricultural productivity with an estimated 50% of arable land predicted to become salinized by 2050. Since most domesticated crops are glycophytes, they cannot be cultivated on salt soils. The use of beneficial microorganisms inhabiting [...] Read more.
Soil salinity is a major abiotic stress in global agricultural productivity with an estimated 50% of arable land predicted to become salinized by 2050. Since most domesticated crops are glycophytes, they cannot be cultivated on salt soils. The use of beneficial microorganisms inhabiting the rhizosphere (PGPR) is a promising tool to alleviate salt stress in various crops and represents a strategy to increase agricultural productivity in salt soils. Increasing evidence underlines that PGPR affect plant physiological, biochemical, and molecular responses to salt stress. The mechanisms behind these phenomena include osmotic adjustment, modulation of the plant antioxidant system, ion homeostasis, modulation of the phytohormonal balance, increase in nutrient uptake, and the formation of biofilms. This review focuses on the recent literature regarding the molecular mechanisms that PGPR use to improve plant growth under salinity. In addition, very recent -OMICs approaches were reported, dissecting the role of PGPR in modulating plant genomes and epigenomes, opening up the possibility of combining the high genetic variations of plants with the action of PGPR for the selection of useful plant traits to cope with salt stress conditions. Full article
(This article belongs to the Special Issue Responses of Plants to Environmental Stresses Volume II)
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22 pages, 1962 KiB  
Article
Comparative Amino Acid Profile and Antioxidant Activity in Sixteen Plant Extracts from Transylvania, Romania
by Andreea Maria Iordache, Constantin Nechita, Paula Podea, Niculina Sonia Șuvar, Cornelia Mesaroṣ, Cezara Voica, Ramona Bleiziffer and Monica Culea
Plants 2023, 12(11), 2183; https://doi.org/10.3390/plants12112183 - 31 May 2023
Cited by 9 | Viewed by 2138
Abstract
In addition to the naturopathic medicines based on the antiseptic, anti-inflammatory, anticancer, or antioxidant properties of plant extracts that have been capitalized upon through the pharmaceutical industry, the increasing interest of the food industry in this area requires potent new materials capable of [...] Read more.
In addition to the naturopathic medicines based on the antiseptic, anti-inflammatory, anticancer, or antioxidant properties of plant extracts that have been capitalized upon through the pharmaceutical industry, the increasing interest of the food industry in this area requires potent new materials capable of supporting this market. This study aimed to evaluate the in vitro amino acid contents and antioxidant activities of ethanolic extracts from sixteen plants. Our results show high accumulated amino acid contents, mainly of proline, glutamic, and aspartic acid. The most consistent values of essential amino acids were isolated from T. officinale, U. dioica, C. majus, A. annua, and M. spicata. The results of the 2,2-diphenyl-1-pycrylhydrazyl (DPPH) radical scavenging assay indicate that R. officinalis was the most potent antioxidant, followed by four other extracts (in decreasing order): T. serpyllum, C. monogyna, S. officinalis, and M. koenigii. The network and principal component analyses found four natural groupings between samples based on DPPH free radical scavenging activity content. Each plant extracts’ antioxidant action was discussed based on similar results found in the literature, and a lower capacity was observed for most species. An overall ranking of the analyzed plant species can be accomplished due to the range of experimental methods. The literature review revealed that these natural antioxidants represent the best side-effect-free alternatives to synthetic additives, especially in the food processing industry. Full article
(This article belongs to the Special Issue Structural and Functional Analysis of Extracts in Plants III)
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13 pages, 508 KiB  
Article
Comparative Study of the Nutritional and Chemical Composition of New Oil Rape, Safflower and Mustard Seed Varieties Developed and Grown in Serbia
by Zorica S. Stojanović, Dajana D. Uletilović, Snežana Ž. Kravić, Žarko S. Kevrešan, Nada L. Grahovac, Ivana S. Lončarević, Ana D. Đurović and Ana M. Marjanović Jeromela
Plants 2023, 12(11), 2160; https://doi.org/10.3390/plants12112160 - 30 May 2023
Cited by 4 | Viewed by 3065
Abstract
Oilseed crops are widely cultivated and are related to nutrition and human health as valuable nutraceutical sources with valuable biological properties. The growing demand for oil plants used in human and animal nutrition or for the processing industry has contributed to the diversification [...] Read more.
Oilseed crops are widely cultivated and are related to nutrition and human health as valuable nutraceutical sources with valuable biological properties. The growing demand for oil plants used in human and animal nutrition or for the processing industry has contributed to the diversification and development of a new variety of oil crops. Increased oil crop diversity, besides ensuring reduced sensitivity to pests and climate conditions, has also led to improved nutritional values. In order to enable oil crop cultivation to become commercially sustainable, a comprehensive characterization of newly created varieties of oilseeds, including their nutritional and chemical composition, is required. In this study, two varieties of safflower and white and black mustard were investigated as alternative oil species for nutritional parameters, mainly protein, fat, carbohydrate, moisture, ash, polyphenols, flavonoids, chlorophylls contents, acids and mineral composition, and compared with those of two different genotypes of rapeseeds as a traditional oil crop plant. The proximate analysis found that the highest oil content was found in the oil rape NS Svetlana genotype (33.23%), while the lowest was in black mustard (25.37%). The protein content varies from around 26% in safflower samples to 34.63%, determined in white mustard. High content of unsaturated fatty acids and low content of saturated fatty acid was observed in the analyzed samples. In mineral analysis, the dominant elements were phosphorus, potassium, calcium and magnesium, in descending order. The observed oil crops are also good sources of microelements, including iron, copper, manganese and zinc, accompanied by high antioxidant activity due to the presence of significant amounts of polyphenolic and flavonoid compounds. Full article
(This article belongs to the Special Issue Plant Nutrition Volume II)
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26 pages, 1464 KiB  
Review
Plant Tolerance to Drought Stress with Emphasis on Wheat
by Sarah Adel and Nicolas Carels
Plants 2023, 12(11), 2170; https://doi.org/10.3390/plants12112170 - 30 May 2023
Cited by 4 | Viewed by 2375
Abstract
Environmental stresses, such as drought, have negative effects on crop yield. Drought is a stress whose impact tends to increase in some critical regions. However, the worldwide population is continuously increasing and climate change may affect its food supply in the upcoming years. [...] Read more.
Environmental stresses, such as drought, have negative effects on crop yield. Drought is a stress whose impact tends to increase in some critical regions. However, the worldwide population is continuously increasing and climate change may affect its food supply in the upcoming years. Therefore, there is an ongoing effort to understand the molecular processes that may contribute to improving drought tolerance of strategic crops. These investigations should contribute to delivering drought-tolerant cultivars by selective breeding. For this reason, it is worthwhile to review regularly the literature concerning the molecular mechanisms and technologies that could facilitate gene pyramiding for drought tolerance. This review summarizes achievements obtained using QTL mapping, genomics, synteny, epigenetics, and transgenics for the selective breeding of drought-tolerant wheat cultivars. Synthetic apomixis combined with the msh1 mutation opens the way to induce and stabilize epigenomes in crops, which offers the potential of accelerating selective breeding for drought tolerance in arid and semi-arid regions. Full article
(This article belongs to the Special Issue Abiotic Stress of Crops: Molecular Genetics and Genomics)
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13 pages, 10152 KiB  
Article
Tomato and Pepper Leaf Parts Contribute Differently to the Absorption of Foliar-Applied Potassium Dihydrogen Phosphate
by Jon Niklas Henningsen, Héctor Alejandro Bahamonde, Karl Hermann Mühling and Victoria Fernández
Plants 2023, 12(11), 2152; https://doi.org/10.3390/plants12112152 - 29 May 2023
Cited by 4 | Viewed by 1931
Abstract
Foliar fertilisation is an application technique that is increasingly being used in agriculture and offers the possibility of providing nutrients directly to the site of highest demand. Especially for phosphorus (P), foliar application is an interesting alternative to soil fertilisation, but foliar uptake [...] Read more.
Foliar fertilisation is an application technique that is increasingly being used in agriculture and offers the possibility of providing nutrients directly to the site of highest demand. Especially for phosphorus (P), foliar application is an interesting alternative to soil fertilisation, but foliar uptake mechanisms are poorly understood. To gain a better understanding of the importance of leaf surface features for foliar P uptake, we conducted a study with tomato (Solanum lycopersicum) and pepper (Capsicum annuum) plants, which have different leaf surface traits. For this purpose, drops of 200 mM KH2PO4 without surfactant were applied onto the adaxial or abaxial leaf side or to the leaf veins and the rate of foliar P absorption was evaluated after one day. Additionally, leaf surfaces were characterised in detail by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), estimating also leaf surface wettability and free energy, among other parameters. While the leaves of pepper hardly contained any trichomes, the abaxial side and the leaf veins of tomato leaves were densely covered with trichomes. The cuticle of tomato leaves was thin (approximately 50 nm), while that of pepper was thick (approximately 150–200 nm) and impregnated with lignin. Due to the fact that trichomes were most abundant in the leaf veins of tomato, dry foliar fertiliser drop residues were observed to be anchored there, and the highest P uptake occurred via tomato leaf veins, resulting in 62% increased P concentration. However, in pepper, the highest rate of P absorption was recorded after abaxial-side P treatment (+66% P). Our results provide evidence that different leaf parts contribute unequally to the absorption of foliar-applied agrochemicals, which could potentially be useful for optimising foliar spray treatments in different crops. Full article
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13 pages, 2201 KiB  
Article
Mineral Nutrient Uptake, Accumulation, and Distribution in Cunninghamia lanceolata in Response to Drought Stress
by Shubin Li, Li Yang, Xiaoyan Huang, Zhiguang Zou, Maxiao Zhang, Wenjuan Guo, Shalom Daniel Addo-Danso and Lili Zhou
Plants 2023, 12(11), 2140; https://doi.org/10.3390/plants12112140 - 29 May 2023
Cited by 5 | Viewed by 1670
Abstract
Mineral accumulation in plants under drought stress is essential for drought tolerance. The distribution, survival, and growth of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.), an evergreen conifer, can be affected by climate change, particularly seasonal precipitation and drought. Hence, we designed a [...] Read more.
Mineral accumulation in plants under drought stress is essential for drought tolerance. The distribution, survival, and growth of Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.), an evergreen conifer, can be affected by climate change, particularly seasonal precipitation and drought. Hence, we designed a drought pot experiment, using 1-year-old Chinese fir plantlets, to evaluate drought effects under simulated mild drought, moderate drought, and severe drought, which corresponds to 60%, 50%, and 40% of soil field maximum moisture capacity, respectively. A treatment of 80% of soil field maximum moisture capacity was used as control. Effects of drought stress on mineral uptake, accumulation, and distribution in Chinese fir organs were determined under different drought stress regimes for 0–45 days. Severe drought stress significantly increased phosphorous (P) and potassium (K) uptake at 15, 30 and 45 days, respectively, within fine (diameter < 2 mm), moderate (diameter 2–5 mm), and large (diameter 5–10 mm) roots. Drought stress decreased magnesium (Mg) and manganese (Mn) uptake by fine roots and increased iron (Fe) uptake in fine and moderate roots but decreased Fe uptake in large roots. Severe drought stress increased P, K, calcium (Ca), Fe, sodium (Na), and aluminum (Al) accumulation in leaves after 45 days and increased Mg and Mn accumulation after 15 days. In stems, severe drought stress increased P, K, Ca, Fe, and Al in the phloem, and P, K, Mg, Na, and Al in the xylem. In branches, P, K, Ca, Fe, and Al concentrations increased in the phloem, and P, Mg, and Mn concentrations increased in the xylem under severe drought stress. Taken together, plants develop strategies to alleviate the adverse effects of drought stress, such as promoting the accumulation of P and K in most organs, regulating minerals concentration in the phloem and xylem, to prevent the occurrence of xylem embolism. The important roles of minerals in response to drought stress should be further evaluated. Full article
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17 pages, 2564 KiB  
Article
Protective Role of an Extract Waste Product from Citrus bergamia in an In Vitro Model of Neurodegeneration
by Jessica Maiuolo, Francesca Bosco, Lorenza Guarnieri, Saverio Nucera, Stefano Ruga, Francesca Oppedisano, Luigi Tucci, Carolina Muscoli, Ernesto Palma, Angelo Maria Giuffrè and Vincenzo Mollace
Plants 2023, 12(11), 2126; https://doi.org/10.3390/plants12112126 - 27 May 2023
Cited by 7 | Viewed by 1729
Abstract
A balanced diet, rich in fruits and vegetables and ensuring the intake of natural products, has been shown to reduce or prevent the occurrence of many chronic diseases. However, the choice to consume large quantities of fruits and vegetables leads to an increase [...] Read more.
A balanced diet, rich in fruits and vegetables and ensuring the intake of natural products, has been shown to reduce or prevent the occurrence of many chronic diseases. However, the choice to consume large quantities of fruits and vegetables leads to an increase in the amount of waste, which can cause an alteration in environmental sustainability. To date, the concept of a “byproduct” has evolved, now being understood as a waste product from which it is still possible obtain useful compounds. Byproducts in the agricultural sector are a rich source of bioactive compounds, capable of possessing a second life, decreasing the amount of waste products, the disposal costs, and environmental pollution. A promising and well-known citrus of the Mediterranean diet is the bergamot (Citrus bergamia, Risso et Poiteau). The composition of bergamot is known, and the rich presence of phenolic compounds and essential oils has justified the countless beneficial properties found, including anti-inflammatory, antioxidant, anti-cholesterolemic, and protective activity for the immune system, heart failure, and coronary heart diseases. The industrial processing of bergamot fruits leads to the formation of bergamot juice and bergamot oil. The solid residues, referred to as “pastazzo”, are normally used as feed for livestock or pectin production. The fiber of bergamot (BF) can be obtained from pastazzo and could exert an interesting effect thanks to its content of polyphenols. The aims of this work were twofold: (a) to have more information (composition, polyphenol and flavonoid content, antioxidant activity, etc.) on BF powder and (b) to verify the effects of BF on an in vitro model of neurotoxicity induced by treatment with amyloid beta protein (Aβ). In particular, a study of cell lines was carried out on both neurons and oligodendrocytes, to measure the involvement of the glia and compare it with that of the neurons. The results obtained showed that BF powder contains polyphenols and flavonoids and that it is able to exercise an antioxidant property. Moreover, BF exerts a protective action on the damage induced by treatment with Aβ, and this defense is found in experiments on the cell viability, on the accumulation of reactive oxygen species, on the involvement of the expression of caspase-3, and on necrotic or apoptotic death. In all these results, oligodendrocytes were always more sensitive and fragile than neurons. Further experiments are needed, and if this trend is confirmed, BF could be used in AD; at the same time, it could help to avoid the accumulation of waste products. Full article
(This article belongs to the Special Issue Use of Waste Products from Fruits and Vegetables)
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21 pages, 5691 KiB  
Review
Transcriptional and Post-Translational Regulation of Plant bHLH Transcription Factors during the Response to Environmental Stresses
by Yasmina Radani, Rongxue Li, Harriet Mateko Korboe, Hongyu Ma and Liming Yang
Plants 2023, 12(11), 2113; https://doi.org/10.3390/plants12112113 - 26 May 2023
Cited by 6 | Viewed by 1883
Abstract
Over the past decades, extensive research has been conducted to identify and characterize various plant transcription factors involved in abiotic stress responses. Therefore, numerous efforts have been made to improve plant stress tolerance by engineering these transcription factor genes. The plant basic Helix–Loop–Helix [...] Read more.
Over the past decades, extensive research has been conducted to identify and characterize various plant transcription factors involved in abiotic stress responses. Therefore, numerous efforts have been made to improve plant stress tolerance by engineering these transcription factor genes. The plant basic Helix–Loop–Helix (bHLH) transcription factor family represents one of the most prominent gene families and contains a bHLH motif that is highly conserved in eukaryotic organisms. By binding to specific positions in promoters, they activate or repress the transcription of specific response genes and thus affect multiple variables in plant physiology such as the response to abiotic stresses, which include drought, climatic variations, mineral deficiencies, excessive salinity, and water stress. The regulation of bHLH transcription factors is crucial to better control their activity. On the one hand, they are regulated at the transcriptional level by other upstream components; on the other hand, they undergo various modifications such as ubiquitination, phosphorylation, and glycosylation at the post-translational level. Modified bHLH transcription factors can form a complex regulatory network to regulate the expression of stress response genes and thus determine the activation of physiological and metabolic reactions. This review article focuses on the structural characteristics, classification, function, and regulatory mechanism of bHLH transcription factor expression at the transcriptional and post-translational levels during their responses to various abiotic stress conditions. Full article
(This article belongs to the Special Issue Abiotic Stress Signaling and Responses in Plants)
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12 pages, 322 KiB  
Article
Alfalfa, Cabbage, Beet and Fennel Microgreens in Floating Hydroponics—Perspective Nutritious Food?
by Sanja Fabek Uher, Sanja Radman, Nevena Opačić, Mia Dujmović, Božidar Benko, Denis Lagundžija, Valent Mijić, Lucija Prša, Srđan Babac and Jana Šic Žlabur
Plants 2023, 12(11), 2098; https://doi.org/10.3390/plants12112098 - 25 May 2023
Cited by 4 | Viewed by 2064
Abstract
Microgreens are young plants of various vegetables, medicinal and aromatic plants, cereals and edible wild plants that were first associated with nouvelle cuisine as decoration in dishes due to their attractive appearance and strong flavor. Recently, they have become more sought after in [...] Read more.
Microgreens are young plants of various vegetables, medicinal and aromatic plants, cereals and edible wild plants that were first associated with nouvelle cuisine as decoration in dishes due to their attractive appearance and strong flavor. Recently, they have become more sought after in the market due to their high nutritional value. This is due to the growing interest of consumers in a healthy lifestyle that includes a varied diet with emphasis on fresh, functional foods. Nowadays, commercial production of microgreens is shifting to modern hydroponic systems due to their numerous advantages, such as accelerated plant growth and biomass production, earlier harvesting, and more production cycles that positively affect yield and chemical composition. Therefore, the aim of this study was to determine the content of specialized metabolites and antioxidant capacity of hydroponically grown alfalfa (Medicago sativa) cv. ‘Kangaroo’, yellow beet (Beta vulgaris var. conditiva) cv. ‘Yellow Lady’, red cabbage (Brassica oleracea L. var. rubra) cv. ‘Red Carpet’, and fennel (Foeniculum vulgare) cv. ‘Aganarpo’ microgreens. The highest content of total phenols (408.03 mg GAE/100 g fw), flavonoids (214.47 mg GAE/100 g fw), non-flavonoids (193.56 mg GAE/100 g fw) and ascorbic acid (74.94 mg/100 g fw) was found in fennel microgreens. The highest content of all analyzed chlorophyll pigments (Chl_a 0.536 mg/g fw, Chl_b 0.248 mg/g fw, and TCh 0.785 mg/g fw) was found in alfalfa microgreens. However, in addition to alfalfa, high levels of chlorophyll a (0.528 mg/g fw), total chlorophyll (0.713 mg/g fw) and the highest level of total carotenoids (0.196 mg/g fw) were also detected in fennel microgreens. The results suggest that microgreens grown on perlite in floating hydroponics have high nutritional potential as a functional food important for human health and therefore could be recommended for daily diet. Full article
23 pages, 15771 KiB  
Article
A High-Precision Plant Disease Detection Method Based on a Dynamic Pruning Gate Friendly to Low-Computing Platforms
by Yufei Liu, Jingxin Liu, Wei Cheng, Zizhi Chen, Junyu Zhou, Haolan Cheng and Chunli Lv
Plants 2023, 12(11), 2073; https://doi.org/10.3390/plants12112073 - 23 May 2023
Cited by 5 | Viewed by 1766
Abstract
Timely and accurate detection of plant diseases is a crucial research topic. A dynamic-pruning-based method for automatic detection of plant diseases in low-computing situations is proposed. The main contributions of this research work include the following: (1) the collection of datasets for four [...] Read more.
Timely and accurate detection of plant diseases is a crucial research topic. A dynamic-pruning-based method for automatic detection of plant diseases in low-computing situations is proposed. The main contributions of this research work include the following: (1) the collection of datasets for four crops with a total of 12 diseases over a three-year history; (2) the proposition of a re-parameterization method to improve the boosting accuracy of convolutional neural networks; (3) the introduction of a dynamic pruning gate to dynamically control the network structure, enabling operation on hardware platforms with widely varying computational power; (4) the implementation of the theoretical model based on this paper and the development of the associated application. Experimental results demonstrate that the model can run on various computing platforms, including high-performance GPU platforms and low-power mobile terminal platforms, with an inference speed of 58 FPS, outperforming other mainstream models. In terms of model accuracy, subclasses with a low detection accuracy are enhanced through data augmentation and validated by ablation experiments. The model ultimately achieves an accuracy of 0.94. Full article
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23 pages, 2187 KiB  
Review
Image-Based High-Throughput Phenotyping in Horticultural Crops
by Alebel Mekuriaw Abebe, Younguk Kim, Jaeyoung Kim, Song Lim Kim and Jeongho Baek
Plants 2023, 12(10), 2061; https://doi.org/10.3390/plants12102061 - 22 May 2023
Cited by 7 | Viewed by 4069
Abstract
Plant phenotyping is the primary task of any plant breeding program, and accurate measurement of plant traits is essential to select genotypes with better quality, high yield, and climate resilience. The majority of currently used phenotyping techniques are destructive and time-consuming. Recently, the [...] Read more.
Plant phenotyping is the primary task of any plant breeding program, and accurate measurement of plant traits is essential to select genotypes with better quality, high yield, and climate resilience. The majority of currently used phenotyping techniques are destructive and time-consuming. Recently, the development of various sensors and imaging platforms for rapid and efficient quantitative measurement of plant traits has become the mainstream approach in plant phenotyping studies. Here, we reviewed the trends of image-based high-throughput phenotyping methods applied to horticultural crops. High-throughput phenotyping is carried out using various types of imaging platforms developed for indoor or field conditions. We highlighted the applications of different imaging platforms in the horticulture sector with their advantages and limitations. Furthermore, the principles and applications of commonly used imaging techniques, visible light (RGB) imaging, thermal imaging, chlorophyll fluorescence, hyperspectral imaging, and tomographic imaging for high-throughput plant phenotyping, are discussed. High-throughput phenotyping has been widely used for phenotyping various horticultural traits, which can be morphological, physiological, biochemical, yield, biotic, and abiotic stress responses. Moreover, the ability of high-throughput phenotyping with the help of various optical sensors will lead to the discovery of new phenotypic traits which need to be explored in the future. We summarized the applications of image analysis for the quantitative evaluation of various traits with several examples of horticultural crops in the literature. Finally, we summarized the current trend of high-throughput phenotyping in horticultural crops and highlighted future perspectives. Full article
(This article belongs to the Special Issue Plant Phenomics for Precision Agriculture)
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13 pages, 1216 KiB  
Article
The Influence of Chestnut Extract and Its Components on Antibacterial Activity against Staphylococcus aureus
by Sara Štumpf, Gregor Hostnik, Tomaž Langerholc, Maša Pintarič, Zala Kolenc and Urban Bren
Plants 2023, 12(10), 2043; https://doi.org/10.3390/plants12102043 - 20 May 2023
Cited by 4 | Viewed by 1613
Abstract
Increasing antimicrobial resistance has caused a great interest in natural products as alternatives or potentiators of antibiotics. The objective of this study was to isolate individual tannins from crude chestnut extract as well as to determine the influence of both crude extracts (tannic [...] Read more.
Increasing antimicrobial resistance has caused a great interest in natural products as alternatives or potentiators of antibiotics. The objective of this study was to isolate individual tannins from crude chestnut extract as well as to determine the influence of both crude extracts (tannic acid extract, chestnut extract) and individual pure tannins (gallic acid, vescalin, vescalagin, castalin, castalagin) on the growth of Gram-positive Staphylococcus aureus bacteria. Their antibacterial activity was monitored by measuring the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) as well as the duration of the lag phase, growth rate and generation time. The effect of growth medium strength on the MIC of different tannins was also investigated. Bacterial growth was followed spectrophotometrically, and MIC values were determined by the microdilution method. The MIC values of various isolated compounds allowed us to determine the bioactive compounds and their contribution to antimicrobial activity. It was found that MIC values increase with increasing growth medium strength and that the lag phase lengthens with increasing tannin concentrations, while the growth rates decrease. Comparing the results of the two studies, the antimicrobial activity of tannins against S. aureus was not as pronounced as in the case of E. coli, which may indicate that a different mechanism of action is responsible for the antimicrobial effects of tannins on Gram-positive than on Gram-negative bacteria, or that a different mechanism is more pronounced. Full article
(This article belongs to the Special Issue Phytochemical Composition and Biological Activity)
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17 pages, 3881 KiB  
Article
Transcriptome and Metabolome Analysis of Selenium Treated Alfalfa Reveals Influence on Phenylpropanoid Biosynthesis to Enhance Growth
by Fengdan Wang, Jie Yang, Yi Hua, Kexin Wang, Yue Guo, Yalin Lu, Siqi Zhu, Pan Zhang and Guofu Hu
Plants 2023, 12(10), 2038; https://doi.org/10.3390/plants12102038 - 19 May 2023
Cited by 4 | Viewed by 1590
Abstract
Selenium (Se) plays an important role in the growth of plants. Alfalfa (Medicago sativa L.) is a perennial legume forage crop with high nutritional value and Se-rich functions. Many studies have shown that selenium can promote alfalfa growth, but few have explored [...] Read more.
Selenium (Se) plays an important role in the growth of plants. Alfalfa (Medicago sativa L.) is a perennial legume forage crop with high nutritional value and Se-rich functions. Many studies have shown that selenium can promote alfalfa growth, but few have explored the molecular biology mechanisms behind this effect. In this study, alfalfa was divided into two groups. One group was sprayed with sodium selenite (Na2SeO3) and the other group was sprayed with distilled water as a control. This study determined the growth, reproductive traits, physiological changes, transcriptome and metabolome of both groups of alfalfa. We found that foliar spraying of 100 mg/L Na2SeO3 could significantly increase the growth rate, dry weight, total Se content, amount of pollen per flower, pollen viability, pod spirals, and seed number per pod of alfalfa plants. The level of chlorophyll, soluble protein, proline, and glutathione also increased dramatically in Na2SeO3-sprayed alfalfa seedlings. After transcriptome and metabolome analysis, a total of 614 differentially expressed genes (DEGs) and 1500 differentially expressed metabolites (DEMs), including 26 secondary differentially metabolites were identified. The DEGs were mainly enriched in MAPK signaling pathway, phenylpropanoid biosynthesis, isoflavonoid biosynthesis, cutin, suberine, and wax biosynthesis, and glycerolipid metabolism. The DEMs were mainly enriched in flavone and flavonol biosynthesis, carbon metabolism, glyoxylate and dicarboxylate metabolism, nitrogen metabolism, and phenylpropanoid biosynthesis. Integrative analysis of transcriptome and metabolome showed that the foliar spraying of Na2SeO3 mainly affects phenylpropanoid biosynthesis to promote alfalfa growth. Full article
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42 pages, 8517 KiB  
Review
Leguminous Seedborne Pathogens: Seed Health and Sustainable Crop Management
by Eliana Dell’Olmo, Antonio Tiberini and Loredana Sigillo
Plants 2023, 12(10), 2040; https://doi.org/10.3390/plants12102040 - 19 May 2023
Cited by 5 | Viewed by 2446
Abstract
Pulses have gained popularity over the past few decades due to their use as a source of protein in food and their favorable impact on soil fertility. Despite being essential to modern agriculture, these species face a number of challenges, such as agronomic [...] Read more.
Pulses have gained popularity over the past few decades due to their use as a source of protein in food and their favorable impact on soil fertility. Despite being essential to modern agriculture, these species face a number of challenges, such as agronomic crop management and threats from plant seed pathogens. This review’s goal is to gather information on the distribution, symptomatology, biology, and host range of seedborne pathogens. Important diagnostic techniques are also discussed as a part of a successful process of seed health certification. Additionally, strategies for sustainable control are provided. Altogether, the data collected are suggested as basic criteria to set up a conscious laboratory approach. Full article
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12 pages, 3106 KiB  
Article
Comparative Metabolomic Analysis Reveals the Role of OsHPL1 in the Cold-Induced Metabolic Changes in Rice
by Ziwei Wu, Zhiyu Guo, Kemiao Wang, Rui Wang and Chuanying Fang
Plants 2023, 12(10), 2032; https://doi.org/10.3390/plants12102032 - 19 May 2023
Cited by 12 | Viewed by 1386
Abstract
Cytochrome P450 (CYP74) family members participate in the generation of oxylipins and play essential roles in plant adaptation. However, the metabolic reprogramming mediated by CYP74s under cold stress remains largely unexplored. Herein, we report how cold-triggered OsHPL1, a member of [...] Read more.
Cytochrome P450 (CYP74) family members participate in the generation of oxylipins and play essential roles in plant adaptation. However, the metabolic reprogramming mediated by CYP74s under cold stress remains largely unexplored. Herein, we report how cold-triggered OsHPL1, a member of the CYP74 family, modulates rice metabolism. Cold stress significantly induced the expression of OsHPL1 and the accumulation of OPDA (12-oxo-phytodienoic acid) and jasmonates in the wild-type (WT) plants. The absence of OsHPL1 attenuates OPDA accumulation to a low temperature. Then, we performed a widely targeted metabolomics study covering 597 structurally annotated compounds. In the WT and hpl1 plants, cold stress remodeled the metabolism of lipids and amino acids. Although the WT and hpl1 mutants shared over one hundred cold-affected differentially accumulated metabolites (DAMs), some displayed distinct cold-responding patterns. Furthermore, we identified 114 and 56 cold-responding DAMs, specifically in the WT and hpl1 mutants. In conclusion, our work characterized cold-triggered metabolic rewiring and the metabolic role of OsHPL1 in rice. Full article
(This article belongs to the Special Issue Metabolism and Stress in Plants)
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32 pages, 7731 KiB  
Article
Foliar Applications of Salicylic Acid on Boosting Salt Stress Tolerance in Sour Passion Fruit in Two Cropping Cycles
by Thiago Galvão Sobrinho, André Alisson Rodrigues da Silva, Geovani Soares de Lima, Vera Lúcia Antunes de Lima, Vitória Ediclécia Borges, Kheila Gomes Nunes, Lauriane Almeida dos Anjos Soares, Luciano Marcelo Fallé Saboya, Hans Raj Gheyi, Josivanda Palmeira Gomes, Pedro Dantas Fernandes and Carlos Alberto Vieira de Azevedo
Plants 2023, 12(10), 2023; https://doi.org/10.3390/plants12102023 - 18 May 2023
Cited by 6 | Viewed by 1684
Abstract
Brazil stands out as the largest producer of sour passion fruit; however, the water available for irrigation is mostly saline, which can limit its cultivation. This study was carried out with the objective of evaluating the effects of salicylic acid in the induction [...] Read more.
Brazil stands out as the largest producer of sour passion fruit; however, the water available for irrigation is mostly saline, which can limit its cultivation. This study was carried out with the objective of evaluating the effects of salicylic acid in the induction of tolerance in sour passion fruit to salt stress. The assay was conducted in a protected environment, using a completely randomized design in a split-plot scheme, with the levels of electrical conductivity of the irrigation water (0.8, 1.6, 2.4, 3.2, and 4.0 dS m−1) considering the plots and concentrations of salicylic acid (0, 1.2, 2.4, and 3.6 mM) the subplots, with three replications. The physiological indices, production components, and postharvest quality of sour passion fruit were negatively affected by the increase in the electrical conductivity of irrigation water, and the effects of salt stress were intensified in the second cycle. In the first cycle, the foliar application of salicylic acid at concentrations between 1.0 and 1.4 mM partially reduced the harmful effects of salt stress on the relative water content of leaves, electrolyte leakage, gas exchange, and synthesis of photosynthetic pigments, in addition to promoting an increase in the yield and quality parameters of sour passion fruit. Full article
(This article belongs to the Special Issue Strategies to Improve Water-Use Efficiency in Plant Production)
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17 pages, 1070 KiB  
Review
The Role of Blue and Red Light in the Orchestration of Secondary Metabolites, Nutrient Transport and Plant Quality
by Alice Trivellini, Stefania Toscano, Daniela Romano and Antonio Ferrante
Plants 2023, 12(10), 2026; https://doi.org/10.3390/plants12102026 - 18 May 2023
Cited by 7 | Viewed by 2666
Abstract
Light is a fundamental environmental parameter for plant growth and development because it provides an energy source for carbon fixation during photosynthesis and regulates many other physiological processes through its signaling. In indoor horticultural cultivation systems, sole-source light-emitting diodes (LEDs) have shown great [...] Read more.
Light is a fundamental environmental parameter for plant growth and development because it provides an energy source for carbon fixation during photosynthesis and regulates many other physiological processes through its signaling. In indoor horticultural cultivation systems, sole-source light-emitting diodes (LEDs) have shown great potential for optimizing growth and producing high-quality products. Light is also a regulator of flowering, acting on phytochromes and inducing or inhibiting photoperiodic plants. Plants respond to light quality through several light receptors that can absorb light at different wavelengths. This review summarizes recent progress in our understanding of the role of blue and red light in the modulation of important plant quality traits, nutrient absorption and assimilation, as well as secondary metabolites, and includes the dynamic signaling networks that are orchestrated by blue and red wavelengths with a focus on transcriptional and metabolic reprogramming, plant productivity, and the nutritional quality of products. Moreover, it highlights future lines of research that should increase our knowledge to develop tailored light recipes to shape the plant characteristics and the nutritional and nutraceutical value of horticultural products. Full article
(This article belongs to the Special Issue Light and Plant Nutrition)
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20 pages, 1529 KiB  
Review
New Strategies to Increase the Abiotic Stress Tolerance in Woody Ornamental Plants in Mediterranean Climate
by Luca Leotta, Stefania Toscano, Antonio Ferrante, Daniela Romano and Alessandra Francini
Plants 2023, 12(10), 2022; https://doi.org/10.3390/plants12102022 - 18 May 2023
Cited by 6 | Viewed by 2118
Abstract
The native flora of different Mediterranean countries, often woody species, was widely recognized for its ornamental potential. The shrubs, in particular, are a typology of plants very widespread in the Mediterranean environment and constituent the ‘Macchia’, the typical vegetation of this ecosystem. The [...] Read more.
The native flora of different Mediterranean countries, often woody species, was widely recognized for its ornamental potential. The shrubs, in particular, are a typology of plants very widespread in the Mediterranean environment and constituent the ‘Macchia’, the typical vegetation of this ecosystem. The use of native shrubs for the realization of ornamental green areas has been recently examined for their adaptability to abiotic stress. Abiotic stresses, in fact, are the major limiting growth factor in urban and peri-urban areas. The identification and use of tolerant ornamental species allow the reduction of management costs and preserve the aesthetical value of green areas. Tolerance to drought stress, for instance, in the Mediterranean climate can improve the ecosystem services of these plants in the urban environment. In this review, the possibility to early individuate different plant species’ mechanisms to tolerate or avoid the stresses is analysed, as well as the possibility to increase abiotic stress tolerance through genetic and agronomic strategies. The exploration of wild or spontaneous species can be a good source for selecting tolerant plants to be used as ornamental plants in urban areas. Among agronomic strategies, biostimulants, mulching, and plant combination can provide a useful solution to counteract abiotic stress in the urban environment. Full article
(This article belongs to the Special Issue Floriculture and Landscape Architecture)
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19 pages, 2818 KiB  
Article
Comparison of Phytochemical Composition and Untargeted Metabolomic Analysis of an Extract from Cnidoscolus aconitifolius (Mill.) I. I. Johnst and Porophyllum ruderale (Jacq.) Cass. and Biological Cytotoxic and Antiproliferative Activity In Vitro
by Ángel Félix Vargas-Madriz, Ivan Luzardo-Ocampo, Ulisses Moreno-Celis, Octavio Roldán-Padrón, Jorge Luis Chávez-Servín, Haydé A. Vergara-Castañeda, Mónica Martínez-Pacheco, Carmen Mejía, Teresa García-Gasca and Aarón Kuri-García
Plants 2023, 12(10), 1987; https://doi.org/10.3390/plants12101987 - 15 May 2023
Cited by 4 | Viewed by 2081
Abstract
Cnidoscolus aconitifolius (CA) and Porophyllum ruderale (PR) are representative edible plants that are a traditional food source in Mexico. This research aimed to analyze the phytochemical composition and untargeted metabolomics analysis of CA and PR and evaluate their antiproliferative effect in vitro. The [...] Read more.
Cnidoscolus aconitifolius (CA) and Porophyllum ruderale (PR) are representative edible plants that are a traditional food source in Mexico. This research aimed to analyze the phytochemical composition and untargeted metabolomics analysis of CA and PR and evaluate their antiproliferative effect in vitro. The phytochemical composition (UPLC-DAD-QToF/MS-ESI) identified up to 38 polyphenols and selected organic acids that were clustered by the untargeted metabolomics in functional activities linked to indolizidines, pyridines, and organic acids. Compared with PR, CA displayed a higher reduction in the metabolic activity of human SW480 colon adenocarcinoma cells (LC50: 10.65 mg/mL), and both extracts increased the total apoptotic cells and arrested cell cycle at G0/G1 phase. PR increased mRNA Apc gene expression, whereas both extracts reduced mRNA Kras expression. Rutin/epigallocatechin gallate displayed the highest affinity to APC and K-RAS proteins in silico. Further research is needed to experiment on other cell lines. Results suggested that CA and PR are polyphenol-rich plant sources exhibiting antiproliferative effects in vitro. Full article
(This article belongs to the Special Issue Plant Extracts and Their Cytotoxic Activities)
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16 pages, 9819 KiB  
Article
Genome-Wide Identification and Expression Analysis of CCO Gene Family in Liriodendron chinense
by Guoxia Xue, Lingfeng Hu, Liming Zhu, Ya Chen, Chen Qiu, Ruifang Fan, Xiaoxiao Ma, Zijian Cao, Jinhui Chen, Jisen Shi and Zhaodong Hao
Plants 2023, 12(10), 1975; https://doi.org/10.3390/plants12101975 - 14 May 2023
Cited by 5 | Viewed by 1969
Abstract
Carotenoid cleavage oxygenase (CCO) is an enzyme that can catalyze carotenoids to volatile aromatic substances and participate in the biosynthesis of two important phytohormones, i.e., abscisic acid (ABA) and strigolactone (SL). However, the genome-wide identification and analysis of the CCO gene family in [...] Read more.
Carotenoid cleavage oxygenase (CCO) is an enzyme that can catalyze carotenoids to volatile aromatic substances and participate in the biosynthesis of two important phytohormones, i.e., abscisic acid (ABA) and strigolactone (SL). However, the genome-wide identification and analysis of the CCO gene family in the rare and endangered woody plant Liriodendron chinense has not been reported. Here, we performed a genome-wide analysis of the CCO gene family in the L. chinense genome and examined its expression pattern during different developmental processes and in response to various abiotic stresses. A total of 10 LcCCO genes were identified and divided into 6 subfamilies according to the phylogenetic analysis. Subcellular localization prediction showed that most of the LcCCO proteins were located in the cytoplasm. Gene replication analysis showed that segmental and tandem duplication contributed to the expansion of this gene family in the L. chinense genome. Cis-element prediction showed that cis-elements related to plant hormones, stress and light response were widely distributed in the promoter regions of LcCCO genes. Gene expression profile analysis showed that LcNCED3b was extensively involved in somatic embryogenesis, especially the somatic embryo maturation, as well as in response to heat and cold stress in leaves. Furthermore, qRT-PCR analysis showed that LcNCED3b obviously responded to drought stress in roots and leaves. This study provides a comprehensive overview of the LcCCO gene family and a potential gene target for the optimization of the somatic embryogenesis system and resistance breeding in the valuable forest tree L. chinense. Full article
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11 pages, 1055 KiB  
Article
Genetic Divergence and Population Structure of Xanthomonas albilineans Strains Infecting Saccharum spp. Hybrid and Saccharum officinarum
by Zhong-Ting Hu, Mbuya Sylvain Ntambo, Jian-Ying Zhao, Talha Javed, Yang Shi, Hua-Ying Fu, Mei-Ting Huang and San-Ji Gao
Plants 2023, 12(10), 1937; https://doi.org/10.3390/plants12101937 - 9 May 2023
Cited by 4 | Viewed by 1503
Abstract
Leaf scald caused by Xanthomonas albilineans (Xa) is a major bacterial disease in sugarcane that represents a threat to the global sugar industry. Little is known about the population structure and genetic evolution of this pathogen. In this study, 39 Xa [...] Read more.
Leaf scald caused by Xanthomonas albilineans (Xa) is a major bacterial disease in sugarcane that represents a threat to the global sugar industry. Little is known about the population structure and genetic evolution of this pathogen. In this study, 39 Xa strains were collected from 6 provinces in China. Of these strains, 15 and 24 were isolated from Saccharum spp. hybrid and S. officinarum plants, respectively. Based on multilocus sequence analysis (MLSA), with five housekeeping genes, these strains were clustered into two distinct phylogenetic groups (I and II). Group I included 26 strains from 2 host plants, Saccharum spp. hybrid and S. officinarum collected from 6 provinces, while Group II consisted of 13 strains from S. officinarum plants in the Zhejiang province. Among the 39 Xa strains, nucleotide sequence identities from 5 housekeeping genes were: ABC (99.6–100%), gyrB (99.3–100%), rpoD (98.4–100%), atpD (97.0–100%), and glnA (97.6–100%). These strains were clustered into six groups (A–F), based on the rep-PCR fingerprinting, using primers for ERIC2, BOX A1R, and (GTG)5. UPGMA and PCoA analyses revealed that group A had the most strains (24), followed by group C with 11 strains, while there was 1 strain each in groups B and D–F. Neutral tests showed that the Xa population in S. officinarum had a trend toward population expansion. Selection pressure analysis showed purification selection on five concatenated housekeeping genes from all tested strains. Significant genetic differentiation and infrequent gene flow were found between two Xa populations hosted in Saccharum spp. hybrids and S. officinarum. Altogether, these results provide evidence of obvious genetic divergence and population structures among Xa strains from China. Full article
(This article belongs to the Special Issue Detection and Diagnostics of Bacterial Plant Pathogens)
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17 pages, 1791 KiB  
Review
Terrestrial and Floating Aquatic Plants Differ in Acclimation to Light Environment
by Marina López-Pozo, William W. Adams III, Stephanie K. Polutchko and Barbara Demmig-Adams
Plants 2023, 12(10), 1928; https://doi.org/10.3390/plants12101928 - 9 May 2023
Cited by 6 | Viewed by 1863
Abstract
The ability of plants to respond to environmental fluctuations is supported by acclimatory adjustments in plant form and function that may require several days and development of a new leaf. We review adjustments in photosynthetic, photoprotective, and foliar vascular capacity in response to [...] Read more.
The ability of plants to respond to environmental fluctuations is supported by acclimatory adjustments in plant form and function that may require several days and development of a new leaf. We review adjustments in photosynthetic, photoprotective, and foliar vascular capacity in response to variation in light and temperature in terrestrial plants. The requirement for extensive acclimation to these environmental conditions in terrestrial plants is contrasted with an apparent lesser need for acclimation to different light environments, including rapid light fluctuations, in floating aquatic plants for the duckweed Lemna minor. Relevant features of L. minor include unusually high growth rates and photosynthetic capacities coupled with the ability to produce high levels of photoprotective xanthophylls across a wide range of growth light environments without compromising photosynthetic efficiency. These features also allow L. minor to maximize productivity and avoid problems during an abrupt experimental transfer of low-light-grown plants to high light. The contrasting responses of land plants and floating aquatic plants to the light environment further emphasize the need of land plants to, e.g., experience light fluctuations in their growth environment before they induce acclimatory adjustments that allow them to take full advantage of natural settings with such fluctuations. Full article
(This article belongs to the Special Issue Photosynthesis under Environmental Fluctuations)
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14 pages, 1009 KiB  
Article
Productivity of Three Pea (Pisum sativum L.) Varieties as Influenced by Nutrient Supply and Meteorological Conditions in Boreal Environmental Zone
by Daiva Janusauskaite
Plants 2023, 12(10), 1938; https://doi.org/10.3390/plants12101938 - 9 May 2023
Cited by 7 | Viewed by 1934
Abstract
In order to grow crops that reduce the negative impact on the environment, as well as meet the nutritional needs of the increasing human population, it is necessary to include new and more sustainable production strategies into current agricultural systems. The aim of [...] Read more.
In order to grow crops that reduce the negative impact on the environment, as well as meet the nutritional needs of the increasing human population, it is necessary to include new and more sustainable production strategies into current agricultural systems. The aim of our study was to evaluate the optimal nutritional conditions of semi-leafless pea productivity and ascertain the influence of meteorological factors on the productivity of these plants under boreal environmental conditions. The test involved three semi-leafless pea varieties, one of which was a new variety, and eight N fertilization treatments were used: (1) without fertilizers (N0), (2) without N fertilizers (N0), (3) N15, (4) N30, (5) N45, (6) N15+15, (7) N60, and (8) N60. Plots of the second–seventh treatment received a base application of P40K80; the eighth treatment received P80K160. Fertilizer efficiency depended on the meteorological conditions. Based on their productivity, the pea varieties were arranged in the following descending order: Ieva DS ˃ Respect ˃ Simona. Compared with unfertilized peas, NPK fertilizers enhanced the seed yield by 10.6–12.9% on average. Splitting the N30 rate and applying N60, under a background of P40K80, was not efficient. The optimal rate of N15–45P40K80 fertilizers for peas was determined. Meteorological factors significantly influenced seed yield by 75.2%, 44.1%, and 79.9% for varieties Ieva DS, Simona, and Respect, respectively. Full article
(This article belongs to the Special Issue Strategies for Nutrient Use Efficiency Improvement in Plants)
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17 pages, 3629 KiB  
Article
Trunk Water Potential Measured with Microtensiometers for Managing Water Stress in “Gala” Apple Trees
by Luis Gonzalez Nieto, Annika Huber, Rui Gao, Erica Casagrande Biasuz, Lailiang Cheng, Abraham D. Stroock, Alan N. Lakso and Terence L. Robinson
Plants 2023, 12(9), 1912; https://doi.org/10.3390/plants12091912 - 8 May 2023
Cited by 6 | Viewed by 1856
Abstract
The weather variations around the world are already having a profound impact on agricultural production. This impacts apple production and the quality of the product. Through agricultural precision, growers attempt to optimize both yield and fruit size and quality. Two experiments were conducted [...] Read more.
The weather variations around the world are already having a profound impact on agricultural production. This impacts apple production and the quality of the product. Through agricultural precision, growers attempt to optimize both yield and fruit size and quality. Two experiments were conducted using field-grown “Gala” apple trees in Geneva, NY, USA, in 2021 and 2022. Mature apple trees (Malus × domestica Borkh. cv. Ultima “Gala”) grafted onto G.11 rootstock planted in 2015 were used for the experiment. Our goal was to establish a relationship between stem water potential (Ψtrunk), which was continuously measured using microtensiometers, and the growth rate of apple fruits, measured continuously using dendrometers throughout the growing season. The second objective was to develop thresholds for Ψtrunk to determine when to irrigate apple trees. The economic impacts of different irrigation regimes were evaluated. Three different water regimes were compared (full irrigation, rainfed and rain exclusion to induce water stress). Trees subjected the rain-exclusion treatment were not irrigated during the whole season, except in the spring (April and May; 126 mm in 2021 and 100 mm in 2022); that is, these trees did not receive water during June, July, August and half of September. Trees subjected to the rainfed treatment received only rainwater (515 mm in 2021 and 382 mm in 2022). The fully irrigated trees received rain but were also irrigated by drip irrigation (515 mm in 2021 and 565 mm in 2022). Moreover, all trees received the same amount of water out of season in autumn and winter (245 mm in 2021 and 283 mm in 2022). The microtensiometer sensors detected differences in Ψtrunk among our treatments over the entire growing season. In both years, experimental trees with the same trunk cross-section area (TCSA) were selected (23–25 cm−2 TCSA), and crop load was adjusted to 7 fruits·cm−2 TCSA in 2021 and 8.5 fruits·cm−2 TCSA in 2022. However, the irrigated trees showed the highest fruit growth rates and final fruit weight (157 g and 70 mm), followed by the rainfed only treatment (132 g and 66 mm), while the rain-exclusion treatment had the lowest fruit growth rate and final fruit size (107 g and 61 mm). The hourly fruit shrinking and swelling rate (mm·h−1) measured with dendrometers and the hourly Ψtrunk (bar) measured with microtensiometers were correlated. We developed a logistic model to correlate Ψtrunk and fruit growth rate (g·h−1), which suggested a critical value of −9.7 bars for Ψtrunk, above which there were no negative effects on fruit growth rate due to water stress in the relatively humid conditions of New York State. A support vector machine model and a multiple regression model were developed to predict daytime hourly Ψtrunk with radiation and VPD as input variables. Yield and fruit size were converted to crop value, which showed that managing water stress with irrigation during dry periods improved crop value in the humid climate of New York State. Full article
(This article belongs to the Special Issue Remote and Proximal Sensing for Plant Research)
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21 pages, 11067 KiB  
Article
Effects of Allium hookeri Extracts on Hair-Inductive and Anti-Oxidative Properties in Human Dermal Papilla Cells
by Seokmuk Park, Nayeon Han, Jung-Min Lee, Jae-Ho Lee and Seunghee Bae
Plants 2023, 12(9), 1919; https://doi.org/10.3390/plants12091919 - 8 May 2023
Cited by 5 | Viewed by 2310
Abstract
Oxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the Allium hookeri extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it was [...] Read more.
Oxidative stress and cellular senescence in dermal papilla cells (DPCs) are major etiological factors causing hair loss. In this study, the effect of the Allium hookeri extract (AHE) on hair-inductive and anti-oxidative properties was investigated in human DPCs. As a result, it was found that a non-cytotoxic concentration of the extracts increased the viability and size of the human DPC spheroid, which was associated with the increased expression of hair-growth-related genes in cells. To determine whether or not these effects could be attributed to intracellular anti-oxidative effects, liquid chromatography-mass spectrometry alongside various biochemical analyses are conducted herein. An ingredient called alliin was identified as one of the main components. Furthermore, AHE treatment induced a significant decrease in H2O2-mediated cytotoxicities, cell death, and cellular senescence in human DPCs. Upon analyzing these results with a molecular mechanism approach, it was shown that AHE treatment increased β-Catenin and NRF2 translocation into the nucleus while inhibiting the translocation of NF-κB (p50) through p38 and PKA-mediated phosphorylations of GSK3β, an upstream regulator of those proteins. These results overall indicate the possibility that AHE can regulate GSK3β-mediated β-Catenin, NRF2, and NF-κB signaling to enhance hair-inductive properties and ultimately protect against oxidative stress-induced cellular damage in human DPCs. Full article
(This article belongs to the Special Issue Bioprospecting of Natural Products from Medicinal Plants)
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15 pages, 3057 KiB  
Article
Differences in Organic Solute and Metabolites of Leymus chinensis in Response to Different Intensities of Salt and Alkali Stress
by Ge Yan, Yujie Shi, Chunsheng Mu and Junfeng Wang
Plants 2023, 12(9), 1916; https://doi.org/10.3390/plants12091916 - 8 May 2023
Cited by 6 | Viewed by 1271
Abstract
To explore differences in the physiological metabolic response mechanisms of grassland perennial plants to different intensities of salt–alkali stress, we employed GC-MS to identify the metabolome of perennial rhizome-saline-tolerant Leymus chinensis (L. chinensis). L. chinensis reduced stress damage by accumulating osmotic [...] Read more.
To explore differences in the physiological metabolic response mechanisms of grassland perennial plants to different intensities of salt–alkali stress, we employed GC-MS to identify the metabolome of perennial rhizome-saline-tolerant Leymus chinensis (L. chinensis). L. chinensis reduced stress damage by accumulating osmotic solutes during salt–alkali stress, although the types of accumulated solutes varied with stress and concentration gradients. Soluble sugars increased only under mild salt–alkali stress. Under salt and mild alkali stress, amino acids increased. Under severe salt–alkali stress, organic acids increased. Betaine increased as a typical osmolute under salt–alkali stress. Metabolic analysis identified 20 metabolites, including 4 amino acids, 6 sugars, and 10 organic acids. The majority of them increased in response to stress. Under mild salt stress, the metabolites included glycine and proline. Under mild alkali stress, they primarily consisted of sugars such as isomaltose and lactulose, whereas under severe salt–alkali stress, they primarily consisted of organic acids such as citric acid and isocitric acid. Pathway analysis showed that six pathways were affected. Glycine, serine, and threonine metabolism was affected under mild salt stress. Alanine, aspartate, and glutamate metabolism and butanota metabolism were affected under mild alkali stress, while energy metabolism pathways, such as the TCA cycle and glyoxylate and dicarboxylate metabolism, were affected under severe salt–alkali stress. The results indicate the importance of betaine in stress resistance and the significance of organic acid in severe salt stress, and they also demonstrate that energy supply was one of the key mechanisms in response to severe salt–alkali stress. Full article
(This article belongs to the Section Plant Physiology and Metabolism)
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23 pages, 7132 KiB  
Article
Macro and Micro-Nutrient Accumulation and Partitioning in Soybean Affected by Water and Nitrogen Supply
by Ingrid Silva Setubal, Aderson Soares de Andrade Júnior, Silvestre Paulino da Silva, Artenisa Cerqueira Rodrigues, Aurenívia Bonifácio, Evandro Henrique Figueiredo Moura da Silva, Paulo Fernando de Melo Jorge Vieira, Rafael de Souza Miranda, Nicolas Cafaro La Menza and Henrique Antunes de Souza
Plants 2023, 12(9), 1898; https://doi.org/10.3390/plants12091898 - 6 May 2023
Cited by 4 | Viewed by 1692
Abstract
This study aimed to investigate the influence of water availability and nitrogen fertilization on plant growth, nutrient dynamics, and variables related to soybean crop yield. Trials were performed in Teresina, Piauí, Brazil, using randomized blocks in a split-split plot arrangement. The plots corresponded [...] Read more.
This study aimed to investigate the influence of water availability and nitrogen fertilization on plant growth, nutrient dynamics, and variables related to soybean crop yield. Trials were performed in Teresina, Piauí, Brazil, using randomized blocks in a split-split plot arrangement. The plots corresponded to water regimes (full and deficient), the split plots to N fertilization (0 and 1000 kg ha−1 N-urea), and the split-split plots to harvest times of soybean plants (16, 23, 30, 37, 44, 58, 65, 79 and 86 days after emergence), with three replicates. In general, the accumulation and partitioning of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), magnesium (Mg), sulphur (S), copper (Cu), iron (Fe), manganese (Mn), zinc (Zn) and boron (B) were decreased in plants subjected to water deficit and without N fertilization. Although nitrogen fertilization promoted elevated N accumulation in tissues, it did not result in any significant yield gain, and the highest seed yields were found in plants under full irrigation, regardless of N supplementation. However, deficient irrigation decreased the seed oil content of N-fertilized plants. In conclusion, N fertilization is critical for nutrient homeostasis, and water availability impairs biomass and nutrient accumulation, thereby limiting soybean yield performance. Full article
(This article belongs to the Special Issue Molecular Basis of Crops and Fruit Plants in Response to Stress)
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23 pages, 4362 KiB  
Article
Arbuscular Mycorrhizal Fungi and Compost-Based Biostimulants Enhance Fitness, Physiological Responses, Yield, and Quality Traits of Drought-Stressed Tomato Plants
by Fatima Ezzahra Soussani, Abderrahim Boutasknit, Raja Ben-Laouane, Rachid Benkirane, Marouane Baslam and Abdelilah Meddich
Plants 2023, 12(9), 1856; https://doi.org/10.3390/plants12091856 - 30 Apr 2023
Cited by 9 | Viewed by 2731
Abstract
Climate change-driven water resource constraints cause tomatoes to suffer from drought. The use of biostimulants has emerged as an important approach to enhancing resilience to drought. However, the roles of biostimulants in the physicochemical characteristics of tomatoes in response to drought are poorly [...] Read more.
Climate change-driven water resource constraints cause tomatoes to suffer from drought. The use of biostimulants has emerged as an important approach to enhancing resilience to drought. However, the roles of biostimulants in the physicochemical characteristics of tomatoes in response to drought are poorly understood. In this study, we evaluated the ability of arbuscular mycorrhizal fungi (AMF) and compost (versus NPK application) to improve the agro-physiology, yield, and fruit quality of tomato plants and their tolerance to drought by comparing them with conventional chemical fertilizers (NPK). Under drought conditions, plant growth traits associated with yield and fruit bioactive compounds (carotenoids: 73%; lycopene: 53%; polyphenols: 310%; and flavonoids: 158%) were increased in the AMF-tomato treatment. Compost significantly enhanced sugars (ca. 60%) and protein contents (ca. 20%). Moreover, AMF protected the photosynthetic apparatus from drought-induced oxidative stress, improved photosynthetic efficiency, leaf water potential, and osmolytes, and reduced malondialdehyde (MDA) and hydrogen peroxide (H2O2) accumulation by increasing peroxidase (POX) (140%) and polyphenol oxidase (PPO) (340%) activities compared to their controls. Our findings revealed that NPK is an important nutrient-based fertilizer for plant growth and development. However, its efficiency as a fertilizer is quite low. In addition, we highlighted different mechanisms mediated by AMF and compost, inducing drought tolerance in tomato plants. Full article
(This article belongs to the Special Issue Crops and Environmental Stresses: Phenomes to Genomes)
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19 pages, 937 KiB  
Review
The Effect of Cadmium on Plants in Terms of the Response of Gene Expression Level and Activity
by Dagmar Moravčíková and Jana Žiarovská
Plants 2023, 12(9), 1848; https://doi.org/10.3390/plants12091848 - 30 Apr 2023
Cited by 5 | Viewed by 2532
Abstract
Cadmium (Cd) is a heavy metal that can cause damage to living organisms at different levels. Even at low concentrations, Cd can be toxic to plants, causing harm at multiple levels. As they are unable to move away from areas contaminated by Cd, [...] Read more.
Cadmium (Cd) is a heavy metal that can cause damage to living organisms at different levels. Even at low concentrations, Cd can be toxic to plants, causing harm at multiple levels. As they are unable to move away from areas contaminated by Cd, plants have developed various defence mechanisms to protect themselves. Hyperaccumulators, which can accumulate and detoxify heavy metals more efficiently, are highly valued by scientists studying plant accumulation and detoxification mechanisms, as they provide a promising source of genes for developing plants suitable for phytoremediation techniques. So far, several genes have been identified as being upregulated when plants are exposed to Cd. These genes include genes encoding transcription factors such as iron-regulated transporter-like protein (ZIP), natural resistance associated macrophage protein (NRAMP) gene family, genes encoding phytochelatin synthases (PCs), superoxide dismutase (SOD) genes, heavy metal ATPase (HMA), cation diffusion facilitator gene family (CDF), Cd resistance gene family (PCR), ATP-binding cassette transporter gene family (ABC), the precursor 1-aminocyclopropane-1-carboxylic acid synthase (ACS) and precursor 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) multigene family are also influenced. Thanks to advances in omics sciences and transcriptome analysis, we are gaining more insights into the genes involved in Cd stress response. Recent studies have also shown that Cd can affect the expression of genes related to antioxidant enzymes, hormonal pathways, and energy metabolism. Full article
(This article belongs to the Special Issue New Insight into Cadmium Phytoremediation)
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