Topic Editors

Dipartimento di Scienze, Università degli Studi della Basilicata, Potenza, Italy
Dipartimento di Scienze, Università degli Studi della Basilicata, 85100 Potenza, Italy
Department of Analysis and Evaluation of Food Quality, University of Life Sciences in Lublin, 8 Skromna Street, 20-704 Lublin, Poland

New Analytical Methods in Plant Active Components Analysis

Abstract submission deadline
31 August 2024
Manuscript submission deadline
31 October 2024
Viewed by
5657

Topic Information

Dear Colleagues,

Plants are well known as one of the greatest resources of active components that are usually used for the development of new drugs, functional foods, and dietary supplements. The discovery of new biologically active compounds from natural sources seems to be the main objective of many pharmaceuticals and food companies. Current research trends clearly indicate that natural active components will be among the most significant sources of new drugs in the future. Due to the complexity of plant active chemical constituents, the development of new robust, efficient, sensitive, and cost-effective methods to improve separation, extraction, and purification of these compounds is of great interest, which may provide the necessary basis for further study of the bioactivity, structure–activity relationships, metabolisms in vivo, structural modification, and synthesis of active compounds. This topic, “New Analytical Methods in Plant Active Component Analysis”, covers a selection of research articles, communications, or reviews that explore plant active compounds’ isolation and structural determination via analytical methods, including chromatography, mass spectrometry, spectroscopy, and electroanalysis. We are particularly interested in articles describing new analytical strategies for determining active compounds in plants, with selected applications linked to current trends in food chemistry, the environment, biology, and pharmaceuticals. Novel applications and the optimization of existing analytical methods are also welcome.

Dr. Filomena Lelario
Dr. Giuliana Bianco
Prof. Dr. Radosław Kowalski
Topic Editors

Keywords

  • chromatography
  • mass spectrometry
  • active compounds
  • natural product extracts
  • separation technology
  • analytical techniques
  • method optimization
  • plants
  • phytochemicals
  • bioactivity
  • plant metabolites
  • structural elucidation
  • hyphenated techniques

Participating Journals

Journal Name Impact Factor CiteScore Launched Year First Decision (median) APC
Analytica
analytica
- - 2020 15.6 Days CHF 1000 Submit
Antioxidants
antioxidants
7.0 8.8 2012 13.9 Days CHF 2900 Submit
Applied Sciences
applsci
2.7 4.5 2011 16.9 Days CHF 2400 Submit
Molecules
molecules
4.6 6.7 1996 14.6 Days CHF 2700 Submit
Separations
separations
2.6 2.5 2014 13.6 Days CHF 2600 Submit

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Published Papers (3 papers)

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16 pages, 2193 KiB  
Article
An Amperometric Biosensor Based on a Bilayer of Electrodeposited Graphene Oxide and Co-Crosslinked Tyrosinase for L-Dopa Detection in Untreated Human Plasma
by Giuseppa Cembalo, Rosanna Ciriello, Carmen Tesoro, Antonio Guerrieri, Giuliana Bianco, Filomena Lelario, Maria Assunta Acquavia and Angela Di Capua
Molecules 2023, 28(13), 5239; https://doi.org/10.3390/molecules28135239 - 06 Jul 2023
Cited by 2 | Viewed by 1118
Abstract
L-Dopa, a bioactive compound naturally occurring in some Leguminosae plants, is the most effective symptomatic drug treatment for Parkinson’s disease. During disease progression, fluctuations in L-DOPA plasma levels occur, causing motor complications. Sensing devices capable of rapidly monitoring drug levels would allow adjusting [...] Read more.
L-Dopa, a bioactive compound naturally occurring in some Leguminosae plants, is the most effective symptomatic drug treatment for Parkinson’s disease. During disease progression, fluctuations in L-DOPA plasma levels occur, causing motor complications. Sensing devices capable of rapidly monitoring drug levels would allow adjusting L-Dopa dosing, improving therapeutic outcomes. A novel amperometric biosensor for L-Dopa detection is described, based on tyrosinase co-crosslinked onto a graphene oxide layer produced through electrodeposition. Careful optimization of the enzyme immobilization procedure permitted to improve the long-term stability while substantially shortening and simplifying the biosensor fabrication. The effectiveness of the immobilization protocol combined with the enhanced performances of electrodeposited graphene oxide allowed to achieve high sensitivity, wide linear range, and a detection limit of 0.84 μM, suitable for L-Dopa detection within its therapeutic window. Interference from endogenous compounds, tested at concentrations levels typically found in drug-treated patients, was not significant. Ascorbic acid exhibited a tyrosinase inhibitory behavior and was therefore rejected from the enzymatic layer by casting an outer Nafion membrane. The proposed device was applied for L-Dopa detection in human plasma, showing good recoveries. Full article
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12 pages, 1357 KiB  
Article
An Interplay between a Face-Centred Composite Experimental Design and Solid-Phase Microextraction for Wine Aroma GC/MS Analysis
by Carmen Tesoro, Maria Assunta Acquavia, Barbara Giussani, Giuliana Bianco, Raffaella Pascale, Filomena Lelario, Rosanna Ciriello, Angela Capece, Rocchina Pietrafesa, Gabriella Siesto and Angela Di Capua
Appl. Sci. 2023, 13(7), 4609; https://doi.org/10.3390/app13074609 - 05 Apr 2023
Viewed by 973
Abstract
For oenological products, most of the intrinsic and extrinsic drivers of perceived quality are associated with specific aromatic profiles. Aromatic diversity has been recognized as a central element in perceived quality as it is able to transmit the complex interactions between grape variety, [...] Read more.
For oenological products, most of the intrinsic and extrinsic drivers of perceived quality are associated with specific aromatic profiles. Aromatic diversity has been recognized as a central element in perceived quality as it is able to transmit the complex interactions between grape variety, geographical characteristics, and viticultural and winemaking practices, including the fermentative process. A comprehensive characterization of flavour compounds by headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometric analysis is often needed in order to ascertain the quality of wine. HS-SPME requires a proper optimization that can be achieved through an adequate experimental design. Here, a HS-SPME/GC-MS based method was developed to investigate the volatile compounds of wine samples obtained by laboratory-scale fermentations. This was performed by inoculating a commercial Saccharomyces cerevisiae strain, which is used both as single starter and as mixed starter, with an indigenous Hanseniaspora osmophila strain. The experimental conditions of HS-SPME (extraction temperature and time) were optimized by applying a face-centred composite experimental design. Up to 95% of the total variance was explained by the proposed model. The optimized method allowed us to confirm the usefulness of combining the inoculation of grapes with selected yeast strains in co-culture situations in order to improve the wine bouquet. Full article
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9 pages, 653 KiB  
Article
Simple and Rapid High-Performance Liquid Chromatography Method for Simultaneous Determination of Picloram and 2,4-D in Pesticide Formulations
by Angela Santilio, Silvana Girolimetti and Valentina Picardo
Analytica 2022, 3(4), 430-438; https://doi.org/10.3390/analytica3040029 - 20 Nov 2022
Cited by 1 | Viewed by 2064
Abstract
Picloram and 2,4-D are systemic herbicides used to control a wide range of broad-leaved weeds and post-emergence annual and perennial broad-leaved weeds, respectively. The use of plant protection products containing Picloram and 2,4-D necessitates the development of analytical methods, useful for the laboratories [...] Read more.
Picloram and 2,4-D are systemic herbicides used to control a wide range of broad-leaved weeds and post-emergence annual and perennial broad-leaved weeds, respectively. The use of plant protection products containing Picloram and 2,4-D necessitates the development of analytical methods, useful for the laboratories focused on control, for monitoring. In this study, we designed and validated an analytical method for the rapid determination of picloram and 2,4-D by HPLC-DAD. The method involves the extraction of the substances by sonication of the sample with methanol, followed by dilution in acetonitrile, and direct injection on a liquid chromatography system, based on the use of a Gemini C18 column. We used an isocratic mobile elution consisting of acetonitrile and water acidified 1% with H3PO4 (50:50, v/v). We validated the proposed method, which demonstrated linearity within the concentration range of 0.01–0.028 mg/mL for picloram and 2,4-D, with a correlation coefficient (R2) of 0.9993 for picloram and 0.9999 for 2,4-D. We considered precision, repeatability and selectivity in the validation. The repeatability of the method expressed as percent of relative standard deviation (%RSD) was lower than 1% for both substances. The proposed method is suitable for the simultaneous determination of picloram and 2,4-D in pesticide formulations. Full article
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Title: Development, validation, and application of HPLC-DAD method for simultaneous determination of ginkgolic acids and ginkgols in ginkgo biloba.
Authors: Isaac Duah Boateng; Fengnan Li; Xiao-Ming Yang
Affiliation: Jiangsu University
Abstract: Ginkgo biloba leaves (GBL), with many phytoconstituents, also contain a toxic substance named ginkgolic acids (GA). Our previous research showed that heating could decarboxylate and degrade GA into ginkgols with enormous bioactivities. Several methods are available to measure GA in GBL, but no analytical method has been developed to measure ginkgols and GA simultaneously. Hence, for the first time, an HPLC-DAD method was established to simultaneously determine GA and ginkgols using acetonitrile (0.01% trifluoroacetic acid, v/v) as mobile phase A, water (0.01% trifluoroacetic acid, v/v) as mobile phase B. Gradient elution conditions were: 0-30 min, 75%-90% phase A; 30-35 min, 90%-90% phase A; 35-36 min, 90%-75% phase A; 36-46 min, 75%-75 % phase A. The detection wavelength of GA and ginkgol was 270 nm. The flow rate and injection volume were 1.0 mL/min and 50 μL, respectively. The linearity was excellent (R2 > 0.999), and the RSD of precision, stability, and repeatability of total ginkgols were 0.20%, 2.21%, and 2.45%, respectively, in six parallel determinations. The recoveries of low, medium, and high groups were 96.58%, 97.67%, and 101.52%, respectively. The limit of detection of ginkgol C13:0, C15:1, and C17:1 were 0.61 ppm, 0.50 ppm, and 0.06 ppm, respectively. Finally, this method was able to accurately measure the GA and ginkgol content in ginkgo leaves and ginkgo tea products and Principal component analysis (PCA) was performed to visualize the association between GA and ginkgols and 5 different processing methods for GBL. Thus, this research provides an efficient and accurate quantitative method for the subsequent detection of GA and ginkgols in ginkgo tea.

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