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Veterinary Sciences
Special Issues
Immunological Assessment of Veterinary Infectious Diseases
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Immunological Assessment of Veterinary Infectious Diseases

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Microbiology, Parasitology and Immunology".

Deadline for manuscript submissions: 25 June 2024 | Viewed by 2915

Special Issue Editors

Prof. Dr. María José García-Iglesias

E-Mail Website
Guest Editor
Department of Animal Health, University of León, 24071 León, Spain
Interests: veterinary pathology; microbiology; porcine respiratory disease complex; antimicrobial resistance; vaccines; immune response; immunohistochemistry
Prof. Dr. César-B. Gutiérrez Martín

E-Mail Website
Guest Editor
Department of Animal Health, University of León, 24071 León, Spain
Interests: veterinary pathology; microbiology; porcine respiratory disease complex; antimicrobial resistance; vaccines; immune response; immunohistochemistry
Special Issues, Collections and Topics in MDPI journals
Dr. Sonia Martinez

E-Mail Website
Guest Editor
Department of Animal Health, University of León, 24071 León, Spain
Interests: veterinary pathology; microbiology; porcine respiratory disease complex; antimicrobial resistance; vaccines; immune response; immunohistochemistry

Special Issue Information

Dear Colleagues,

Infectious diseases are responsible for large economic losses in farm animals, pets, and wildlife. Most of these diseases are contagious, and they result in high animal mortality and morbidity associated with reduced animal growth, treatment costs, and condemnation in organs and carcasses at the slaughterhouse. Moreover, zoonotic diseases represent a danger to human health. The diagnosis of infectious diseases is mainly based on serological tests and cultures of the causative pathogens. Histopathological studies with routine staining or histochemical techniques may also help to identify some pathogens and/or their tissue injury. However, these tools have limitations such as difficulties in interpreting serological results in immunocompromised animals, the unavailability of fresh tissue for culture, and non-specific tissue lesions, among others. In this context, the aim of this Special Issue is to encourage scientists to publish their immunological studies on any infectious disease in animals to better understand the host–pathogen relationships that allow for the improvement and development of novel prophylactic, therapeutic and diagnostic tools.

Prof. Dr. María José García-Iglesias
Prof. Dr. César-B. Gutiérrez Martín
Dr. Sonia Martinez
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • immunological assessment
  • infectious diseases
  • animals

Published Papers (1 paper)

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Research

11 pages, 864 KiB  
Article
An ELISA to Detect Antibodies to Bovine Alphaherpesviruses 1 and 5 and Bubaline Alphaherpesvirus 1 in Cattle Sera
by Camila Mengue Scheffer, Sylio Alfredo Petzhold, Ana Paula Muterle Varela, Willian Pinto Paim, Phelipe Magalhães Duarte, Márcia Regina Loiko, Cristine Cerva, Candice Schmidt, Adrieli Wendlant, Samuel Paulo Cibulski, Diane Alves de Lima, Caroline Tochetto, Anne Caroline Ramos dos Santos, Juliana Inês Herpich, Thais Fumaco Teixeira, Helton Fernandes dos Santos, Fabrício Souza Campos, Ana Cláudia Franco and Paulo Michel Roehe
Vet. Sci. 2023, 10(2), 110; https://doi.org/10.3390/vetsci10020110 - 02 Feb 2023
Cited by 2 | Viewed by 1835
Abstract
Bovine alphaherpesvirus 1 (subtypes 1.1, 1.2a, and 1.2b), type 5 (subtypes 5a, 5b, and 5c), and bubaline herpesvirus 1 (BuHV-1) induce highly, though not fully cross-reactive serological responses. Most types and subtypes of these viruses circulate particularly in countries of the southern hemisphere, [...] Read more.
Bovine alphaherpesvirus 1 (subtypes 1.1, 1.2a, and 1.2b), type 5 (subtypes 5a, 5b, and 5c), and bubaline herpesvirus 1 (BuHV-1) induce highly, though not fully cross-reactive serological responses. Most types and subtypes of these viruses circulate particularly in countries of the southern hemisphere, notably Brazil and Argentina. Therefore, the detection of infected animals is important in defining prevention and control strategies, particularly when flocks are destined for international trade. Identification of infected herds is most often achieved by assays that detect antibodies, such as enzyme immunoassays (ELISAs). However, to date, no ELISA has been evaluated in its capacity to detect antibodies to these alphaherpesviruses. Here, an ELISA was developed to detect antibodies to all currently recognized BoAHV-1, BoAHV-5, and BuAHV-1 types/subtypes, and its sensitivity and specificity were determined. Six hundred bovine sera were screened in serum neutralization tests (SN) against the seven viruses. ELISAs prepared with each of the viruses were compared to SN. Subsequently, a combined assay with multiple antigens LISA was prepared by mixing five viral antigens, chosen for their highest sensitivity in the preparative assays. In comparison to SN, the mAgELISA sensitivity was 96.5% with 96.1% specificity (κ = 0.93; PPV = 95.0%; NPV = 97.3%). The findings reveal that the mAgELISA developed here is highly suitable for the detection of antibodies, comparable in sensitivity and specificity to that of SN when performed with all known types and subtypes of bovine and bubaline alphaherpesviruses. Full article
(This article belongs to the Special Issue Immunological Assessment of Veterinary Infectious Diseases)
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