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Selected Papers from the 15th European Fusarium Seminar

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A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Mycotoxins".

Deadline for manuscript submissions: closed (10 December 2021) | Viewed by 32030

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Prof. Dr. Kris Audenaert

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Department of Plants and Crops, Faculty of Bioscience Engineering, Ghent University, Valentin Vaerwyckweg 1, 9000 Ghent, Belgium
Interests: plant–pathogen interactions; biocontrol; endophytic fungi; green leaf volatiles (GLVs); biogenic volatile compounds (BVOCs); plant defense priming; plant hormones; phenomics; genome-editing in fungi; plant defense pathways; biodegradation of mycotoxins; mycotoxin prediction and modelling; bioassays to assess toxicity of trichothecenes and their derivatives
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Dear Colleagues,

The 15th European Fusarium Seminar, organized by Ghent University, will be held online from May 31 to June 1, 2021.

Fusarium is one of the most important plant pathogenic fungal genera. Economic losses both in monocots and eudicots are due to direct yield losses as well as due to the presence of toxic metabolites called mycotoxins that are produced by many species within the Fusarium genus. The challenges in Fusarium research are still enormous due to the frequency, complexity and variability in the occurrence of this pathogen.

We would like to welcome you to the 15th edition of the European Fusarium Seminar. The program includes oral and poster presentations on topics that provide new insights into the different Fusarium research fields: Fusarium–Host Interactions, Integrated and Innovative Fusarium Management, Secondary Metabolites, Non-Cereal Plant Fusarium Interactions, Population Genetics and New Technologies.

The Symposium welcomes participants from industry, government, as well as academia. Only through our multidisciplinary efforts and concerted actions, can we further progress and expect solutions in the Fusarium research field. This Special Issue aims to bring together active researchers to present their current work. For additional information on the Seminar which you may find useful, please follow: http://en.mytox.be/conferences/

Prof. Dr. Kris Audenaert
Guest Editor

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Keywords

Fusarium population genetics
mitigation strategies
new technologies
plant–host interactions
Fusarium interacting with non-cereal hosts

Published Papers (9 papers)

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Research

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22 pages, 2430 KiB

Open AccessEditor’s ChoiceArticle

Different Resistance to DON versus HT2 + T2 Producers in Nordic Oat Varieties

by Ingerd Skow Hofgaard, Guro Brodal, Marit Almvik, Morten Lillemo, Aina Lundon Russenes, Simon Graham Edwards and Heidi Udnes Aamot

Toxins 2022, 14(5), 313; https://doi.org/10.3390/toxins14050313 - 28 Apr 2022

Cited by 8 | Viewed by 2628

Abstract

Over recent decades, the Norwegian cereal industry has had major practical and financial challenges associated with the occurrence of Fusarium head blight (FHB) pathogens and their associated mycotoxins in cereal grains. Deoxynivalenol (DON) is one of the most common Fusarium-mycotoxins in Norwegian oats, however T-2 toxin (T2) and HT-2 toxin (HT2) are also commonly detected. The aim of our study was to rank Nordic spring oat varieties and breeding lines by content of the most commonly occurring Fusarium mycotoxins (DON and HT2 + T2) as well as by the DNA content of their respective producers. We analyzed the content of mycotoxins and DNA of seven fungal species belonging to the FHB disease complex in grains of Nordic oat varieties and breeding lines harvested from oat field trials located in the main cereal cultivating district in South-East Norway in the years 2011–2020. Oat grains harvested from varieties with a high FHB resistance contained on average half the levels of mycotoxins compared with the most susceptible varieties, which implies that choice of variety may indeed impact on mycotoxin risk. The ranking of oat varieties according to HT2 + T2 levels corresponded with the ranking according to the DNA levels of Fusarium langsethiae, but differed from the ranking according to DON and Fusarium graminearum DNA. Separate tests are therefore necessary to determine the resistance towards HT2 + T2 and DON producers in oats. This creates practical challenges for the screening of FHB resistance in oats as today’s screening focuses on resistance to F. graminearum and DON. We identified oat varieties with generally low levels of both mycotoxins and FHB pathogens which should be preferred to mitigate mycotoxin risk in Norwegian oats. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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22 pages, 19700 KiB

Open AccessArticle

A Novel In Planta Enrichment Method Employing Fusarium graminearum-Infected Wheat Spikes to Select for Competitive Biocontrol Bacteria

by Waldo Deroo, Larissa De Troyer, Fréderic Dumoulin, Sarah De Saeger, Marthe De Boevre, Steven Vandenabeele, Leen De Gelder and Kris Audenaert

Toxins 2022, 14(3), 222; https://doi.org/10.3390/toxins14030222 - 17 Mar 2022

Cited by 12 | Viewed by 3049

Abstract

This work introduces an alternative workflow for the discovery of novel bacterial biocontrol agents in wheat against Fusarium head blight (FHB). Unlike the mass testing of isolate collections, we started from a diverse inoculum by extracting microbiomes from ears of field-grown plants at grain filling stage. Four distinct microbial communities were generated which were exposed to 3 14-day culture-independent experimental enrichments on detached wheat spikes infected with F. graminearum PH1. We found that one bacterial community reduced infection symptoms after 3 cycles, which was chosen to subsequently isolate bacteria through limiting dilution. All 94 isolates were tested in an in vitro and in planta assay, and a selection of 14 isolates was further tested on detached ears. The results seem to indicate that our enrichment approach resulted in bacteria with different modes-of-action in regard to FHB control. Erwinia persicina isolate C3 showed a significant reduction in disease severity (Fv/Fm), and Erwinia persicina C3 and Pseudomonas sp. B3 showed a significant reduction in fungal biomass (cGFP). However, the mycotoxin analysis of both these treatments showed no reduction in DON levels. Nevertheless, Pantoea ananatis H3 and H11 and Erwinia persicina H2 were able to reduce DON concentrations by more than 50%, although these effects were not statistically significant. Lastly, Erwinia persicina H2 also showed a significantly greater glucosylation of DON to the less phytotoxic DON-3G. The bacterial genera isolated through the enrichment cycles have been reported to dominate microbial communities that develop in open habitats, showing strong indications that the isolated bacteria can reduce the infection pressure of F. graminearum on the spike phyllosphere. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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25 pages, 3961 KiB

Open AccessEditor’s ChoiceArticle

Host Genotype and Weather Effects on Fusarium Head Blight Severity and Mycotoxin Load in Spring Barley

by Felix Hoheneder, Eva Maria Biehl, Katharina Hofer, Johannes Petermeier, Jennifer Groth, Markus Herz, Michael Rychlik, Michael Heß and Ralph Hückelhoven

Toxins 2022, 14(2), 125; https://doi.org/10.3390/toxins14020125 - 08 Feb 2022

Cited by 5 | Viewed by 2481

Abstract

Epidemiology of Fusarium Head Blight (FHB) of spring barley is relatively little understood. In a five-year study, we assessed quantitative resistance to FHB in an assortment of 17 spring barley genotypes in the field in southern Germany. To this end, we used soil and spray inoculation of plants with F. culmorum and F. avenaceum. This increased disease pressure and provoked genotypic differentiation. To normalize effects of variable weather conditions across consecutive seasons, we used a disease ranking of the genotypes based on quantification of fungal DNA contents and multiple Fusarium toxins in harvested grain. Together, this allowed for assessment of stable quantitative FHB resistance of barley in several genotypes. Fungal DNA contents were positively associated with species-specific Fusarium toxins in single years and over several years in plots with soil inoculation. In those plots, plant height limited FHB; however, this was not observed after spray inoculation. A multiple linear regression model of recorded weather parameter and fungal DNA contents over five years identified time periods during the reproductive phase of barley, in which weather strongly influenced fungal colonization measured in mature barley grain. Environmental conditions before heading and late after anthesis showed strongest associations with F. culmorum DNA in all genotypes, whereas for F. avenaceum, this was less consistent where we observed weather-dependent associations, depending on the genotype. Based on this study, we discuss aspects of practical resistance breeding in barley relevant to improve quantitative resistance to FHB and associated mycotoxin contaminations. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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16 pages, 3865 KiB

Open AccessEditor’s ChoiceArticle

Taqman qPCR Quantification and Fusarium Community Analysis to Evaluate Toxigenic Fungi in Cereals

by Elina Sohlberg, Vertti Virkajärvi, Päivi Parikka, Sari Rämö, Arja Laitila and Tuija Sarlin

Toxins 2022, 14(1), 45; https://doi.org/10.3390/toxins14010045 - 06 Jan 2022

Cited by 6 | Viewed by 2675

Abstract

Fusarium head blight (FHB) is an economically important plant disease. Some Fusarium species produce mycotoxins that cause food safety concerns for both humans and animals. One especially important mycotoxin-producing fungus causing FHB is Fusarium graminearum. However, Fusarium species form a disease complex where different Fusarium species co-occur in the infected cereals. Effective management strategies for FHB are needed. Development of the management tools requires information about the diversity and abundance of the whole Fusarium community. Molecular quantification assays for detecting individual Fusarium species and subgroups exist, but a method for the detection and quantification of the whole Fusarium group is still lacking. In this study, a new TaqMan-based qPCR method (FusE) targeting the Fusarium-specific elongation factor region (EF1α) was developed for the detection and quantification of Fusarium spp. The FusE method was proven as a sensitive method with a detection limit of 1 pg of Fusarium DNA. Fusarium abundance results from oat samples correlated significantly with deoxynivalenol (DON) toxin content. In addition, the whole Fusarium community in Finnish oat samples was characterized with a new metabarcoding method. A shift from F. culmorum to F. graminearum in FHB-infected oats has been detected in Europe, and the results of this study confirm that. These new molecular methods can be applied in the assessment of the Fusarium community and mycotoxin risk in cereals. Knowledge gained from the Fusarium community analyses can be applied in developing and selecting effective management strategies for FHB. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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9 pages, 607 KiB

Open AccessArticle

Pydiflumetofen Co-Formulated with Prothioconazole: A Novel Fungicide for Fusarium Head Blight and Deoxynivalenol Control

by Simon G. Edwards

Toxins 2022, 14(1), 34; https://doi.org/10.3390/toxins14010034 - 03 Jan 2022

Cited by 6 | Viewed by 2341

Abstract

Fusarium head blight (FHB) is an important disease of small grain cereals worldwide, resulting in reduced yield and quality as well as the contamination of harvested grains with mycotoxins. The key mycotoxin of concern is deoxynivalenol (DON), which has legislative and advisory limits in numerous countries. Cereal growers have a number of control options for FHB including rotation, cultivation, and varietal resistance; however, growers are still reliant on fungicides applied at flowering as part of an IPM program. Fungicides currently available to control FHB are largely restricted to triazole chemistry. This study conducted three field experiments to compare a new co-formulation of pydiflumetofen (a succinate dehydrogenase inhibitor (SDHI) with the tradename ADEPIDYN^™) and prothioconazole (a triazole) against current standard fungicides at various timings (flag leaf fully emerged, mid-head emergence, early flowering, and late flowering) for the control of FHB and DON. Overall, the co-formulation showed greater efficacy compared to either pydiflumetofen alone or current fungicide chemistry. This greater activity was demonstrated over a wide range of spray timings (flag leaf fully emerged to late flowering). The availability of an SDHI with good activity against FHB and the resulting DON contamination of harvested grain will give growers an additional tool within an IPM program that will provide a greater flexibility of spray application windows and reduce fungicide resistance selection pressure. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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14 pages, 1984 KiB

Open AccessEditor’s ChoiceArticle

Naturally Occurring Fusarium Species and Mycotoxins in Oat Grains from Manitoba, Canada

by M. Nazrul Islam, Mourita Tabassum, Mitali Banik, Fouad Daayf, W. G. Dilantha Fernando, Linda J. Harris, Srinivas Sura and Xiben Wang

Toxins 2021, 13(9), 670; https://doi.org/10.3390/toxins13090670 - 18 Sep 2021

Cited by 13 | Viewed by 4489

Abstract

Fusarium head blight (FHB) can lead to dramatic yield losses and mycotoxin contamination in small grain cereals in Canada. To assess the extent and severity of FHB in oat, samples collected from 168 commercial oat fields in the province of Manitoba, Canada, during 2016–2018 were analyzed for the occurrence of Fusarium head blight and associated mycotoxins. Through morphological and molecular analysis, F. poae was found to be the predominant Fusarium species affecting oat, followed by F. graminearum, F. sporotrichioides, F. avenaceum, and F. culmorum. Deoxynivalenol (DON) and nivalenol (NIV), type B trichothecenes, were the two most abundant Fusarium mycotoxins detected in oat. Beauvericin (BEA) was also frequently detected, though at lower concentrations. Close clustering of F. poae and NIV/BEA, F. graminearum and DON, and F. sporotrichioides and HT2/T2 (type A trichothecenes) was detected in the principal component analysis. Sampling location and crop rotation significantly impacted the concentrations of Fusarium mycotoxins in oat. A phylogenetic analysis of 95 F. poae strains from Manitoba was conducted using the concatenated nucleotide sequences of Tef-1α, Tri1, and Tri8 genes. The results indicated that all F. poae strains belong to a monophyletic lineage. Four subgroups of F. poae strains were identified; however, no correlations were observed between the grouping of F. poae strains and sample locations/crop rotations. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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19 pages, 2837 KiB

Open AccessEditor’s ChoiceArticle

Phylogeny and Mycotoxin Profile of Pathogenic Fusarium Species Isolated from Sudden Decline Syndrome and Leaf Wilt Symptoms on Date Palms (Phoenix dactylifera) in Tunisia

by Amal Rabaaoui, Chiara Dall’Asta, Laura Righetti, Antonia Susca, Antonio Francesco Logrieco, Ahmed Namsi, Radhouane Gdoura, Stefaan P. O. Werbrouck, Antonio Moretti and Mario Masiello

Toxins 2021, 13(7), 463; https://doi.org/10.3390/toxins13070463 - 30 Jun 2021

Cited by 17 | Viewed by 4307

Abstract

In 2017–2018, extensive symptoms of sudden decline and fruit rot were observed on date palms in southern Tunisia. Samples of diseased plants were randomly collected in six localities. Based on morphological identification, Fusarium was the most frequent fungal genus detected. A sequencing of translation elongation factor, calmodulin, and second largest subunit of RNA polymerase II genes was used to identify 63 representative Fusarium strains at species level and investigate their phylogenetic relationships. The main species detected was Fusarium proliferatum, and at a much lesser extent, Fusarium brachygibbosum, Fusarium caatingaense, Fusarium clavum, Fusarium incarnatum, and Fusarium solani. Pathogenicity on the Deglet Nour variety plantlets and the capability to produce mycotoxins were also assessed. All Fusarium species were pathogenic complying Koch’s postulates. Fusarium proliferatum strains produced mainly fumonisins (FBs), beauvericin (BEA), and, to a lesser extent, enniatins (ENNs) and moniliformin (MON). All F. brachygibbosum strains produced low levels of BEA, diacetoxyscirpenol, and neosolaniol; two strains produced also T-2 toxin, and a single strain produced HT-2 toxin. Fusarium caatingaense, F. clavum, F. incarnatum produced only BEA. Fusarium solani strains produced MON, BEA, and ENNs. This work reports for the first time a comprehensive multidisciplinary study of Fusarium species on date palms, concerning both phytopathological and food safety issues. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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Review

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28 pages, 4189 KiB

Open AccessEditor’s ChoiceReview

Post-Translational Modifications of Histones Are Versatile Regulators of Fungal Development and Secondary Metabolism

by Aurelie Etier, Fabien Dumetz, Sylvain Chéreau and Nadia Ponts

Toxins 2022, 14(5), 317; https://doi.org/10.3390/toxins14050317 - 29 Apr 2022

Cited by 6 | Viewed by 4064

Abstract

Chromatin structure is a major regulator of DNA-associated processes, such as transcription, DNA repair, and replication. Histone post-translational modifications, or PTMs, play a key role on chromatin dynamics. PTMs are involved in a wide range of biological processes in eukaryotes, including fungal species. Their deposition/removal and their underlying functions have been extensively investigated in yeasts but much less in other fungi. Nonetheless, the major role of histone PTMs in regulating primary and secondary metabolisms of filamentous fungi, including human and plant pathogens, has been pinpointed. In this review, an overview of major identified PTMs and their respective functions in fungi is provided, with a focus on filamentous fungi when knowledge is available. To date, most of these studies investigated histone acetylations and methylations, but the development of new methodologies and technologies increasingly allows the wider exploration of other PTMs, such as phosphorylation, ubiquitylation, sumoylation, and acylation. Considering the increasing number of known PTMs and the full range of their possible interactions, investigations of the subsequent Histone Code, i.e., the biological consequence of the combinatorial language of all histone PTMs, from a functional point of view, are exponentially complex. Better knowledge about histone PTMs would make it possible to efficiently fight plant or human contamination, avoid the production of toxic secondary metabolites, or optimize the industrial biosynthesis of certain beneficial compounds. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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29 pages, 2075 KiB

Open AccessEditor’s ChoiceReview

Secondary Metabolite Gene Regulation in Mycotoxigenic Fusarium Species: A Focus on Chromatin

by Anna Katharina Atanasoff-Kardjalieff and Lena Studt

Toxins 2022, 14(2), 96; https://doi.org/10.3390/toxins14020096 - 25 Jan 2022

Cited by 10 | Viewed by 4753

Abstract

Fusarium is a species-rich group of mycotoxigenic plant pathogens that ranks as one of the most economically important fungal genera in the world. During growth and infection, they are able to produce a vast spectrum of low-molecular-weight compounds, so-called secondary metabolites (SMs). SMs often comprise toxic compounds (i.e., mycotoxins) that contaminate precious food and feed sources and cause adverse health effects in humans and livestock. In this context, understanding the regulation of their biosynthesis is crucial for the development of cropping strategies that aim at minimizing mycotoxin contamination in the field. Nevertheless, currently, only a fraction of SMs have been identified, and even fewer are considered for regular monitoring by regulatory authorities. Limitations to exploit their full chemical potential arise from the fact that the genes involved in their biosynthesis are often silent under standard laboratory conditions and only induced upon specific stimuli mimicking natural conditions in which biosynthesis of the respective SM becomes advantageous for the producer. This implies a complex regulatory network. Several components of these gene networks have been studied in the past, thereby greatly advancing the understanding of SM gene regulation and mycotoxin biosynthesis in general. This review aims at summarizing the latest advances in SM research in these notorious plant pathogens with a focus on chromatin structure. Full article

(This article belongs to the Special Issue Selected Papers from the 15th European Fusarium Seminar)

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