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Separations
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Application of Liquid Chromatography in Pharmaceutical and Toxicological Analysis
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Application of Liquid Chromatography in Pharmaceutical and Toxicological Analysis

A special issue of Separations (ISSN 2297-8739). This special issue belongs to the section "Chromatographic Separations".

Deadline for manuscript submissions: closed (20 March 2024) | Viewed by 5051

Special Issue Editors

Dr. Maria Nieddu

E-Mail Website
Guest Editor
Department of Medicine, Surgery and Pharmacy, University of Sassari, 07100 Sassari, Italy
Interests: liquid chromatography; mass spectrometry detection; UV-detection; sample preparation; method validation; pharmaceutical analysis; forensic analysis; biological matrices; extraction methods
Dr. Elena Baralla

E-Mail Website
Guest Editor
Department of Veterinary Medicine, University of Sassari, 07100 Sassari, Italy
Interests: mass spectrometry; veterinary toxicology; residues analysis; method validation; chromatography; sample preparation
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are pleased to invite you to publish in this Special Issue focused on the application of liquid chromatography in pharmaceutical and toxicological analysis.

In pharmaceutical and toxicological fields, chromatography is one of the most used analytical methods for the identification and quantification of xenobiotics and their metabolites.

Different chromatographic techniques have been developed for the qualitative-quantitative determination of drugs according to their characteristics. Among these techniques, in particular, High-Performance Liquid Chromatography (HPLC) is the most used for pharmaceutical and toxicological purposes. The success of liquid chromatography in the development of rapid and precise analytical methods is that it provides good specificity and sensitivity. Sample pretreatment is an important factor to be considered to provide a selective chromatographic analysis. The use of specific purification techniques provides a sample that is relatively free of interferences, compatible with the analytical method, and that does not damage instrumentation. It is further desirable to concentrate the analytes for improved detection.

This Special Issue aims to provide an overview of applications of liquid chromatography in pharmaceutical and toxicological analysis, including both human and veterinary medicine.

In this Special Issue, original research articles and reviews as well as short communications are welcome. Research areas may include (but are not limited to) the following: liquid chromatography applied to analytical methods for drugs determination in biological matrices or pharmaceutical formulations, forensic and toxicological analysis, residue analysis, sample preparation, and development and validation of new analytical methods.

We look forward to receiving your contributions.

Dr. Maria Nieddu
Dr. Elena Baralla
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • liquid chromatography
  • drug analysis
  • forensic analysis
  • toxicological analysis
  • residues analysis
  • sample preparation
  • method validation
  • biological matrices
  • pharmaceutical preparation

Published Papers (3 papers)

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Research

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13 pages, 1787 KiB  
Article
An LC–MS/MS Analytical Method for Quantifying Tepotinib in Human Liver Microsomes: Application to In Vitro and In Silico Metabolic Stability Estimation
by Mohamed W. Attwa, Gamal A. E. Mostafa, Haitham AlRabiah and Adnan A. Kadi
Separations 2023, 10(6), 330; https://doi.org/10.3390/separations10060330 - 26 May 2023
Cited by 4 | Viewed by 1238
Abstract
Tepotinib (MSC2156119) is a potent mesenchymal–epithelial transition (MET) factor inhibitor, a receptor tyrosine kinase that plays a crucial role in promoting cancer cell malignant progression. Adverse effects of tepotinib (TEP), such as peripheral edema, interstitial lung disease, nausea and diarrhea, occur due to [...] Read more.
Tepotinib (MSC2156119) is a potent mesenchymal–epithelial transition (MET) factor inhibitor, a receptor tyrosine kinase that plays a crucial role in promoting cancer cell malignant progression. Adverse effects of tepotinib (TEP), such as peripheral edema, interstitial lung disease, nausea and diarrhea, occur due to drug accumulation and lead to termination of therapy. Therefore, the in silico and experimental metabolic susceptibility of TEP was investigated. In the current work, an LC-MS/MS analytical method was developed for TEP estimation with metabolic stability assessment. TEP and lapatinib (LTP) used as internal standards (ISs) were separated on a reversed-phase C18 column using the isocratic mobile phase. Protein precipitation steps were used to extract TEP from the human liver microsome (HLM) matrix. An electrospray ionization multi-reaction monitoring (MRM) acquisition was conducted at m/z 493→112 for TEP, at m/z 581→350, and 581→365 for the IS. Calibration was in the range of 5 to 500 ng/mL (R2 = 0.999). The limit of detection (LOD) was 0.4759 ng/mL, whereas the limit of quantification (LOQ) was 1.4421 ng/mL. The reproducibility of the developed analytical method (inter- and intra-day precision and accuracy) was within 4.39%. The metabolic stability of TEP in HLM was successfully assessed using the LC-MS/MS method. The metabolic stability assessment of TEP showed intermediate Clint (35.79 mL/min/kg) and a moderate in vitro t1/2 (22.65 min), proposing the good bioavailability and moderate extraction ratio of TEP. The in silico results revealed that the N-methyl piperidine group is the main reason of TEP metabolic lability. The in silico Star Drop software program could be used in an effective protocol to confirm and propose the practical in vitro metabolic experiments to spare resources and time, especially during the first stages for designing new drugs. The established analytical method is considered the first LC-MS/MS method for TEP estimation in the HLM matrix with its application to metabolic stability assessment. Full article
(This article belongs to the Special Issue Application of Liquid Chromatography in Pharmaceutical and Toxicological Analysis)
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Review

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22 pages, 5053 KiB  
Review
The Application of Liquid Chromatography for the Analysis of Organic UV Filters in Environmental and Marine Biota Matrices
by Maria Nieddu, Valeria Pasciu, Maria Piera Demontis and Elena Baralla
Separations 2024, 11(1), 30; https://doi.org/10.3390/separations11010030 - 12 Jan 2024
Viewed by 1261
Abstract
Sunscreens are topical preparations containing one or more compounds that protect humans from ultraviolet (UV) light. This review focuses on analytical methods, particularly liquid chromatography, with the aim of identifying and determining UV filters (UVFs) in environmental and marine biota matrices. A literature [...] Read more.
Sunscreens are topical preparations containing one or more compounds that protect humans from ultraviolet (UV) light. This review focuses on analytical methods, particularly liquid chromatography, with the aim of identifying and determining UV filters (UVFs) in environmental and marine biota matrices. A literature review was conducted using NIH (PubMed and Medline), FDA and EPA databases, Google Scholar, and federal regulations. This retrospective literature review is focused on the last five years. UVF quantification in environmental and biological matrices showed a wide array of methods where liquid chromatography is predominant. The scientific literature identified a large variety of analytical methodologies that are compared in this review to evaluate the better results in terms of limits of quantification and the possibility to identify as many analytes as possible simultaneously. Full article
(This article belongs to the Special Issue Application of Liquid Chromatography in Pharmaceutical and Toxicological Analysis)
16 pages, 1441 KiB  
Review
A Review of Liquid Chromatography-Mass Spectrometry Strategies for the Analyses of Metabolomics Induced by Microplastics
by Kuan-Lu Wu, Wan-Rou Liao, Wei-Chen Lin and Sung-Fang Chen
Separations 2023, 10(4), 257; https://doi.org/10.3390/separations10040257 - 16 Apr 2023
Viewed by 2059
Abstract
Microplastics (MPs) (microscopic plastic particles) are defined as plastic fragments in the range of 1 to 5 mm. They are invisible and usually too small to be seen by humans but pollution by MPs has become an issue due to the rising use [...] Read more.
Microplastics (MPs) (microscopic plastic particles) are defined as plastic fragments in the range of 1 to 5 mm. They are invisible and usually too small to be seen by humans but pollution by MPs has become an issue due to the rising use of plastic products. Pollution of microscopic plastics has gained international attention in recent years and has become an important issue in the field of environmental protection, food safety, and human health. Numerous studies have reported that MPs have the potential for causing detrimental effects in various species. The focus of this mini review was on LC-MS-based metabolomics research into this issue by targeted and untargeted approaches. We also summarized biomarkers for assessing toxicity in land and aquatic species that are induced by MPs with different sizes and shapes, type of monomer, and the dose. Based on previous research results, MPs have the potential for affecting energy metabolism and the immune system, chronic inflammation, and neurotransmitter disorders in a wide variety of species. These biomarkers discovered by metabolomics are consistent with other methods, showing the reliability of LC-MS-based metabolomics. Further research is highly anticipated to explore other toxicity effects that are induced by MPs. Full article
(This article belongs to the Special Issue Application of Liquid Chromatography in Pharmaceutical and Toxicological Analysis)
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