Dear Colleagues,

For several decades now, we have known that “structure is function”. Of course, we can invert this binomial relation and state that “function is structure”, i.e., each enzyme’s function is carefully regulated by a given fold or a specific set of three-dimensional arrays that tightly control each enzyme’s activity.

Enzymology in itself is a branch of biochemistry that aims to understand this relationship and provide insights into folding pathways, allosteric regulation, and catalytic mechanisms. However, enzymology calls upon a wide range of methods and disciplines providing answers to previously unanswered questions and conundrums. From molecular biology to molecular genetics, from biochemistry to biophysics, and from structural biology to biological chemistry, in no other field like in Enzymology can we gather so much knowledge on a given target regarding all these questions. Using a “from gene to structure” approach, enzymology and structural biology techniques are front-and-center disciplines in present-day science.

In this Special Issue, we will focus on “Applications of Structural Biology Techniques to Enzymology”. We aim to collect contributions covering a wide spectrum of disciplines and techniques providing an overview of the state-of-the-art methods and extensive repertoire of structural biology techniques that contribute to the field of enzymology.

Topics include but are not limited to:

• Gene expression and protein production strategies;
• Sample quality assessment for structural biology applications;
• Biophysical characterization methodologies;
• Biochemical characterization of enzymes and complexes;
• Spectroscopic characterization of metalloenzymes (Mössbauer, Raman, XANES/EXAFS and EPR);
• Structural biology techniques for three-dimensional structure determination (MX, NMR, SAXS and cryo-EM);
• Enzyme function, specificity and catalysis;
• Ways and means in Intrinsically Disorder Protein (ISPs) studies;
• Structure-based enzyme technology for industrial, biomedical, biotechnological, and environmental applications;
• Computational approaches to enzyme’s structure and function;
• Integrative and disruptive methods in enzymology and/or structural biology.

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

• enzymology
• structural biology
• biochemical and biophysical techniques
• metalloenzyme spectroscopies
• intrinsically disorder protein
• structure–function relationship