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Recent Advances in Application of Silver Nanoparticles in Biochemistry
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Recent Advances in Application of Silver Nanoparticles in Biochemistry

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Processes and Systems".

Deadline for manuscript submissions: closed (31 March 2024) | Viewed by 8651

Special Issue Editors

Dr. Ramesa Shafi Bhat

E-Mail Website
Guest Editor
Biochemistry Department, College of Sciences, King Saud University, Riyadh 11495, Saudi Arabia
Interests: biochemistry; green symthesis; nanoparticles
Prof. Dr. Afaf El-Ansary

E-Mail Website
Guest Editor
Autism Center, Lotus Holistic Medical Center, Abu Dhabi, United Arab Emirates
Interests: biochemistry; biomarkers; neurodevelopment
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Nano silver-based materials have shown some interesting characteristics suitable for various biomedical applications, such as antimicrobial, anti-inflammatory, antifungal, antiviral, and anticancer. Their therapeutic activity in the fields of dentistry, orthopedics, cancer therapy, etc. is at the clinical trial stage and receiving tremendous interest. Due to their strong bactericidal effects, silver nanoparticles (AgNPs) are used in catheters, burns, wound dressing, and many pharmacological treatments. AgNPs can significantly reduce inflammation in wounds by modulating fibrogenic cytokines, down-regulating pro-inflammatory cytokines, and helping towards apoptosis in inflammatory cells. AgNPs interact with cancer cells and activate many signaling pathways related to mitochondrial dysfunction, oxidative stress, and autophagy. These nanostructures are receiving special attention in agriculture due to their ability to control plant diseases and increase the growth and yield of crops. They act as growth stimulators in plants by improving biochemicals such as chlorophyll, carbohydrate, protein, and antioxidant enzymes.

This Special Issue on “Recent Advances in Application of Silver Nanoparticles in Biochemistry” aims to cover recent advances in the application of silver nanoparticles in various fields of bioscience.   Topics include but are not limited to methods and/or applications in the following areas:

  • Synthesis and characterization of silver nanoparticles;
  • Silver nanoparticles in the management of plant diseases and agricultural applications;
  • Silver nanoparticles and multidrug-resistant bacterial species;
  • Silver nanoparticles and human health;
  • Cytotoxicity of silver nanoparticles;
  • Silver nanoparticles in neurodevelopment and neurodegeneration.

Dr. Ramesa Shafi Bhat
Prof. Dr. Afaf El-Ansary
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • silver nanoparticles
  • green synthesis
  • antimicrobial
  • anticancer
  • biomedical applications
  • toxicity
  • human health
  • oxidative stress

Published Papers (5 papers)

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Research

15 pages, 4311 KiB  
Article
Role of Biosynthesized Silver Nanoparticles with Trigonella foenum-graecum Seeds in Wastewater Treatment
by Manal A. Awad, Promy Virk, Awatif A. Hendi, Khalid Mustafa Ortashi, Najla AlMasoud and Taghrid S. Alomar
Processes 2023, 11(8), 2394; https://doi.org/10.3390/pr11082394 - 09 Aug 2023
Cited by 3 | Viewed by 1052
Abstract
As the human population continues to escalate, its requirement for clean water is also increasing. This has resulted in an increased dependency on wastewater effluent to maintain the base flow of urban streams, especially in water-stressed regions. The present study reports the synthesis [...] Read more.
As the human population continues to escalate, its requirement for clean water is also increasing. This has resulted in an increased dependency on wastewater effluent to maintain the base flow of urban streams, especially in water-stressed regions. The present study reports the synthesis of AgNPs with green credentials using an aqueous extract of Trigonella foenum-graecum seeds. The observance of surface plasmon resonance (SPR) with UV–Vis spectrophotometry confirmed the presence of spherical/oblong particles with a mean diameter of 43.8 nm and low polydispersity index (PDI) of 0.391 measured by transmission electron microscopy (TEM) and DLS (dynamic light scattering) technique, respectively. The elemental map of AgNPs was demonstrated with energy-dispersive spectroscopy (EDS) and the constituent functional groups were identified by the FTIR spectra, which were similar to the bulk seed extract with a slight shift in the pattern. The emission spectrum of nanoparticles was recorded for the excitation wavelength of 349 using fluorescence microscopy and the crystalline structure was assessed using X-ray diffraction. The potential wastewater remedial efficacy of the synthesized AgNPs was evaluated based on the water quality parameters (pH, EC, BOD, COD) of the sewage effluent collected from a local Sewage Treatment Plant (STP). Furthermore, the photo degradative efficacy was investigated using the degradation percentage of Crystal Violet (CV) dye, which was recorded as 94.5% after 20 min. In addition, the antimicrobial activity of the NPs versus bulk seed extract was assessed against two bacterial strains, Escheria coli and Staphylococcus aureus, using the disc diffusion method. The AgNPs showed a profound modulatory effect on the water quality parameters, coupled with marked antimicrobial and photodegradative activity. Thus, the biogenically synthesized AgNPs offer a prospective potential for use in wastewater remediation strategies. Full article
(This article belongs to the Special Issue Recent Advances in Application of Silver Nanoparticles in Biochemistry)
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15 pages, 4196 KiB  
Article
Investigation of the Antimicrobial and Physico-Mechanical Properties of Nature-Friendly Nanosilver-Loaded Pig Lining Leather Prepared Using Exhaustion Method
by Ngoc-Thang Nguyen, Tien-Hieu Vu, Van-Huan Bui, Duy-Nam Phan, Thi-Hang Nguyen and Thi-My-Linh Nguyen
Processes 2023, 11(7), 1891; https://doi.org/10.3390/pr11071891 - 23 Jun 2023
Cited by 2 | Viewed by 1089
Abstract
The natural hydrophilic property of leather makes it a possible growing substrate for microorganisms. Microorganisms such as bacteria, yeasts, and molds frequently grow on shoe lining leather, causing skin diseases, unpleasant odors, and discomfort for the wearers. To address these issues, herein, bio-synthesized [...] Read more.
The natural hydrophilic property of leather makes it a possible growing substrate for microorganisms. Microorganisms such as bacteria, yeasts, and molds frequently grow on shoe lining leather, causing skin diseases, unpleasant odors, and discomfort for the wearers. To address these issues, herein, bio-synthesized silver nanoparticles from Piper betle L. leaf extract were applied as an antimicrobial agent to pig leather via the exhaustion method. The characterization of nanosilver-treated pig leather was performed using colorimetry, scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), atomic absorption spectroscopy (AAS), and FTIR techniques. Evaluation of antimicrobial efficacy of the nanosilver-treated leather was both qualitatively and quantitatively assessed against two bacteria (Escherichia coli and Staphylococcus aureus) and two fungi (a yeast Candida albicans and a mold Aspergillus niger) in accordance with AATCC TM90, AATCC TM30, and ISO 16187:2013 standards. The results indicated that the nanosilver was immobilized on the surface of collagen fibers as well as within the collagen matrix of the pig leather. The treated leather exhibited highly effective antibacterial and antifungal activities against all tested microorganisms, and the inhibition increased with an increase in the initial nanosilver concentration in the treated solution. Furthermore, the exhaustion technique used for the antimicrobial treatment of pig leather had no negative effects on its physico-mechanical properties, and it met the standard requirements of ISO 20882:2007 for shoe upper lining. Therefore, based on the efficient antimicrobial and suitable physico-mechanical properties, nanosilver-treated pig leather adapts the criteria for making hygienic shoe upper lining. Full article
(This article belongs to the Special Issue Recent Advances in Application of Silver Nanoparticles in Biochemistry)
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18 pages, 4630 KiB  
Article
Rotheca serrata Flower Bud Extract Mediated Bio-Friendly Preparation of Silver Nanoparticles: Their Characterizations, Anticancer, and Apoptosis Inducing Ability against Pancreatic Ductal Adenocarcinoma Cell Line
by Kariyellappa Nagaraja Shashiraj, Sreenivasa Nayaka, Raju Suresh Kumar, Gireesh Babu Kantli, Dhanyakumara Shivapoojar Basavarajappa, Pooja Vidyasagar Gunagambhire, Abdulrahman I. Almansour and Karthikeyan Perumal
Processes 2023, 11(3), 893; https://doi.org/10.3390/pr11030893 - 16 Mar 2023
Cited by 10 | Viewed by 1671
Abstract
Over past decades, the green method of synthesizing metal nanoparticles has acquired more attentiveness by scientific consensus because of its industrial and biomedical applications. This study focuses on the anti-proliferative effectiveness of AgNPs synthesized from Rotheca serrata (L.) Steane & Mabb. flower bud [...] Read more.
Over past decades, the green method of synthesizing metal nanoparticles has acquired more attentiveness by scientific consensus because of its industrial and biomedical applications. This study focuses on the anti-proliferative effectiveness of AgNPs synthesized from Rotheca serrata (L.) Steane & Mabb. flower bud extract against the PANC-1 cell line in vitro. Various analytical instruments were utilized to visualize the formation of RsFb-AgNPs, such as UV-Vis spectroscopy, FT-IR, SEM, EDS, TEM, XRD, Zeta potential, and DLS analysis. The biosynthesis of RsFb-AgNPs was observed by a change in color and UV-Vis spectroscopy (415 nm). The FT-IR spectra exhibited the existence of many functional groups. XRD confirmed the crystallinity of the AgNPs. Morphology and elemental mapping were assessed by SEM and EDS analysis. The TEM micrograph revealed spherical-shaped particles with sizes ranging from 12 to 40 nm. Zeta potential and DLS analysis were used to measure surface charge and particle size. Biological properties, including the antioxidant, antimicrobial, and anticancer properties of synthesized RsFb-AgNPs, exhibited dose-dependent activities. In DPPH assay, synthesized RsFb-AgNPs inhibited the scavenging of free radicals in a dose-dependent manner. In addition, the resultant RsFb-AgNPs displayed moderate antimicrobial activity against tested pathogens. Further, the anti-proliferative efficacy of biosynthesized RsFb-AgNPs was determined against the PANC-1 cell line using the MTT assay. The results revealed a dose-dependent decrease in viability of cancer cells with an IC50 value of 36.01 µg/mL. Flow cytometry was then used to confirm the apoptotic effects by double staining with annexin V/PI. In response to the pancreatic ductal adenocarinoma cell line, the results showed notable early and late apoptosis cell population percentages. In conclusion, the synthesized RsFb-AgNPs revealed a potential anticancer agent that can induce apoptosis in the PANC-1 cells. Full article
(This article belongs to the Special Issue Recent Advances in Application of Silver Nanoparticles in Biochemistry)
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16 pages, 5946 KiB  
Article
Anticancer and Antimicrobial Activity of Silver Nanoparticles Synthesized from Pods of Acacia nilotica
by Nuha Suliman Alduraihem, Ramesa Shafi Bhat, Sabah Ahmed Al-Zahrani, Doaa M. Elnagar, Hussah M. Alobaid and Maha H. Daghestani
Processes 2023, 11(2), 301; https://doi.org/10.3390/pr11020301 - 17 Jan 2023
Cited by 21 | Viewed by 2334
Abstract
Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression [...] Read more.
Green synthesized silver nanoparticles (AgNPs) have been used against antibiotic-resistant bacteria and chemo-resistant cancer cells. We synthesized AgNPs from Acacia nilotica pods, evaluating their antibacterial activity against eight bacterial strains and anticancer efficiency against two colon cancer cell lines, SW620 and SW480. Expression levels of eight genes (β-catenin, APC, TP53, Beclin1, DKK3, Axin, Cyclin D1, and C-myc) were checked by a reverse transcription-polymerase chain reaction in cancer cells before and after treatment with A. nilotica extract and A. nilotica-AgNPs. Prepared nanoparticles were characterized through ultraviolet-visible (UV-vis), Zetasizer, scanning electron microscopy (SEM), and transmission electron microscopy (TEM). Fourier transform infrared spectroscopy (FTIR) was used to identify the functional group in extracts. At first, AgNPs were confirmed by a sharp peak of surface plasmon resonance at 375 nm. The Z-average size was 105.4 nm with a polydispersity index of 0.297. TEM showed particle size of 11–30 nm. The prepared AgNPs showed promising antibacterial activity against bacterial strains and cytotoxic activity against the cancer cell lines. Expression levels of all the genes were affected by extract and AgNPs treatment. Overall, this study recommended both A. nilotica pods and A. nilotica-AgNPs as attractive candidates for antibacterial and anticancer applications. Full article
(This article belongs to the Special Issue Recent Advances in Application of Silver Nanoparticles in Biochemistry)
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22 pages, 10259 KiB  
Article
Phytofabrication of Silver Nanoparticles and Their Potent Antifungal Activity against Phytopathogenic Fungi
by Humaira Rizwana, Tethkar Alzahrani, Mona S. Alwahibi, Reem M. Aljowaie, Horiah A. Aldehaish, Noura S. Alsaggabi and Rasha Ramadan
Processes 2022, 10(12), 2558; https://doi.org/10.3390/pr10122558 - 01 Dec 2022
Cited by 7 | Viewed by 1962
Abstract
Fungal plant pathogens cause huge losses in agricultural production by decreasing crop yield and quality. To reduce crop loss from fungal damage, various synthetic fungicides are applied indiscriminately in agricultural practice. The majority of synthetic fungicides are non-biodegradable, and several critical human health [...] Read more.
Fungal plant pathogens cause huge losses in agricultural production by decreasing crop yield and quality. To reduce crop loss from fungal damage, various synthetic fungicides are applied indiscriminately in agricultural practice. The majority of synthetic fungicides are non-biodegradable, and several critical human health risks are associated with them. Green synthesis nanotechnology offers an effectual, cost-effective, ecofriendly, and innocuous method for the synthesis of green nanofungicides, an excellent replacement for synthetic chemical fungicides. Origanum majorana is an aromatic herb with immense pharmacological and medicinal properties. In this context, the present study used the leaves of O. majorana to synthesize silver nanoparticles. The biosynthesized particles showed an absorption peak at 441 nm with ultraviolet-visible spectrophotometry (UV-Vis). The spectra obtained from Fourier transform infrared spectroscopy (FT-IR) of O. majorana extract and AgNPs showed a myriad of functional groups corresponding to vital biomolecules that act as capping and reducing agents. The synthesized silver nanoparticles were spheroidal, and their size measured between 8 nm and 42 nm, as depicted by transmission electron microscopy (TEM). The energy-dispersive X-ray spectrum (EDX) showed a silver peak at 3 keV. The phytofabricated silver NPs demonstrated robust inhibitory activity on the mycelial growth of A. alternata f sp. lycopersici (87%), followed by Pestalotiopsis mangiferae (85%), Macrophomina phaseolina (78%), and Colletotrichum musae (75%). The minimum inhibitory concentration value for A. alternata. f sp. lycopersici and Pestalotiopsis mangiferae was 2 μg/mL, while the minimum fungicidal concentrations were 4 and 8 μg/mL, respectively. Additionally, the fabricated AgNPs induced severe damaging and destructive effects to the morphology of hyphae and conidia, as witnessed by scanning electron microscopy studies. Full article
(This article belongs to the Special Issue Recent Advances in Application of Silver Nanoparticles in Biochemistry)
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