Chitosan and its Derivatives: Structure, Properties, and Applications

A topical collection in Polymers (ISSN 2073-4360). This collection belongs to the section "Biomacromolecules, Biobased and Biodegradable Polymers".

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Editor

Laboratory of Polymer Chemistry and Technology, Department of Chemistry, Aristotle University of Thessaloniki, GR 54124 Thessaloniki, Greece
Interests: synthesis and characterization of polyesters; development of biobased polymers; biodegradable polymers; polymer composites and nanocomposites; synthesis and characterization of copolymers; polymer blends; recycling of polymers with various techniques; enzymatic hydrolysis studies; modification of natural polymers; polymer for wastewater treatment pollutant removal; polymers for tissue engineering and drug delivery applications; drug–polymer solid dispersions; drug targeting; drug nanoencapsulation and microencapsulation
Special Issues, Collections and Topics in MDPI journals

Topical Collection Information

Dear Colleagues,

Chitosan (CS) is a hemi-synthetic cationic linear polysaccharide consisting of randomly distributed D-glucosamine and N-acetyl-D-glucosamine units, linked via β-(1→4) glycosidic bonds. It is synthesized by the deacetylation of chitin, which is a naturally occurring polysaccharide. CS is water-soluble only in weakly acidic pH (>4.5) due to the protonation of its free amino groups. It is an easily accessible polymer, but most importantly it is non-toxic, highly biocompatible and biodegradable, stable and sterilizable, and with low immunogenicity. CS has a unique hydrophilic character since, along with its polymeric chains, it also contains free water-loving hydroxyl and amino groups, which can be used for its chemical modification. This is an additional crucial advantage, allowing for easy tuning of its physicochemical properties, leading to functionalized derivatives with desired and appropriate features for several applications (biotechnology, medicine, membranes, cosmetics, food industry, etc.).

CS has inherent antibacterial and mucoadhesive properties, and a gel-forming ability, also acting as a permeation enhancer in some cases. Due to these characteristics, CS is an excellent candidate for pharmaceutical, cosmetic, and biomedical applications (micro and nanoparticles, films, membranes, sponges, gels, etc.,) and several CS-containing products have already been approved by the US Food and Drug Administration. It has been extensively used as a single polymer matrix in several drug delivery systems, including oral administration routes, the micro-/nano-encapsulation of drugs for ocular and nasal delivery, and the topical and transdermal delivery systems in the form of patches or gels.

CS and its derivatives have been widely used in regenerative medicine for the development of versatile scaffolds for tissue engineering applications and the preparation of artificial tissues and organs (bones, teeth, cartilage, joints, blood vessels, nerves, skin, etc.).

Due to its functional groups, chitosan and/or its appropriately modified derivatives can be used as a flocculant/coagulant or bioabsorbent in wastewater treatment removing several pollutants such as heavy metal ions, organochloride pesticides, textile wastewater dyes, drugs, fatty and oil impurities, etc., or in drinking water purification.

Composites and nanocomposites of chitosan, and various natural or synthetic polymer chitosan blends are also of high interest for several applications.

Papers exploring the processing of chitosan and its derivatives, their properties, physical and chemical characterization, and their applications are welcome in this Topical Collection.

Prof. Dr. Dimitrios Bikiaris
Collection Editor

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Keywords

  • chitosan
  • polysaccharide
  • natural polymer
  • biobased
  • biodegradable polymer
  • chitosan derivatives
  • nanocomposites
  • blends
  • drug delivery applications
  • biomedical applications
  • tissue engineering applications
  • wastewater treatment
  • food industry

Related Special Issues

Published Papers (50 papers)

2023

Jump to: 2022, 2021, 2020

23 pages, 4036 KiB  
Review
Mechanism of Heterogeneous Alkaline Deacetylation of Chitin: A Review
by Vitaly Yu. Novikov, Svetlana R. Derkach, Irina N. Konovalova, Natalya V. Dolgopyatova and Yulya A. Kuchina
Polymers 2023, 15(7), 1729; https://doi.org/10.3390/polym15071729 - 30 Mar 2023
Cited by 6 | Viewed by 4530
Abstract
This review provides an analysis of experimental results on the study of alkaline heterogeneous deacetylation of chitin obtained by the authors and also published in the literature. A detailed analysis of the reaction kinetics was carried out considering the influence of numerous factors: [...] Read more.
This review provides an analysis of experimental results on the study of alkaline heterogeneous deacetylation of chitin obtained by the authors and also published in the literature. A detailed analysis of the reaction kinetics was carried out considering the influence of numerous factors: reaction reversibility, crystallinity and porosity of chitin, changes in chitin morphology during washing, alkali concentration, diffusion of hydroxide ions, and hydration of reacting particles. A mechanism for the chitin deacetylation reaction is proposed, taking into account its kinetic features in which the decisive role is assigned to the effects of hydration. It has been shown that the rate of chitin deacetylation increases with a decrease in the degree of hydration of hydroxide ions in a concentrated alkali solution. When the alkali concentration is less than the limit of complete hydration, the reaction practically does not occur. Hypotheses have been put forward to explain the decrease in the rate of the reaction in the second flat portion of the kinetic curve. The first hypothesis is the formation of “free” water, leading to the hydration of chitin molecules and a decrease in the reaction rate. The second hypothesis postulates the formation of a stable amide anion of chitosan, which prevents the nucleophilic attack of the chitin macromolecule by hydroxide ions. Full article
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27 pages, 9411 KiB  
Article
Preparation and Characterization of a New Bis-Uracil Chitosan-Based Hydrogel as Efficient Adsorbent for Removal of Anionic Congo Red Dye
by Rana A. Alharbi, Fahad M. Alminderej, Nouf F. Al-Harby, Noura Y. Elmehbad and Nadia A. Mohamed
Polymers 2023, 15(6), 1529; https://doi.org/10.3390/polym15061529 - 20 Mar 2023
Cited by 9 | Viewed by 1384
Abstract
A new hydrogel, based on chitosan crosslinked with 2-chlorophenyl-bis(6-amino-1,3-dimethyluracil-5-yl) methane, (2Clph-BU-Cs), has been successfully created. Various instrumental techniques such as elemental analysis, FTIR, SEM, and XRD were used to prove its structure. Its removal efficiency for anionic Congo red (CR) dye under different [...] Read more.
A new hydrogel, based on chitosan crosslinked with 2-chlorophenyl-bis(6-amino-1,3-dimethyluracil-5-yl) methane, (2Clph-BU-Cs), has been successfully created. Various instrumental techniques such as elemental analysis, FTIR, SEM, and XRD were used to prove its structure. Its removal efficiency for anionic Congo red (CR) dye under different conditions for industrial wastewater treatment was studied. For optimizing the conditions to maximize CR dye removal, the impacts of temperature, contact time, pH, and initial concentration of the dye on adsorption capacity were investigated. The removal of the dye was pH-dependent, with a much higher value achieved at pH 4 than at pH 7 and 9. The maximum adsorption capacity of the hydrogel was 93.46 mg g−1. The model of adsorption process was fitted to the pseudo-second-order kinetic model. The intraparticle diffusion demonstrated the multi-step nature of the adsorption process. The thermodynamic results showed that the adsorption process was endothermic because of the positive value of enthalpy (43.70 kJ mol−1). The process of adsorption at high temperatures was spontaneous, according to the values of ∆G0. An increase in randomness was seen in the value of ∆S°. Generally, the investigated hydrogel has the potential to be used as a promising effective reusable adsorbent for industrial wastewater remediation. Full article
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2022

Jump to: 2023, 2021, 2020

15 pages, 3302 KiB  
Article
Macroporous 3D Chitosan Cryogels for Fastac 10EC Pesticide Adsorption and Antibacterial Applications
by Ionel Adrian Dinu, Luminita Ghimici and Irina Elena Raschip
Polymers 2022, 14(15), 3145; https://doi.org/10.3390/polym14153145 - 02 Aug 2022
Cited by 9 | Viewed by 1609
Abstract
The pesticide pollution of surface water and wastewater has been recognized as a major worldwide concern due to their persistence in the aquatic environment and the potential adverse effects on human, flora, and fauna health. Apart from pesticides, bio-contamination with various bacterial populations [...] Read more.
The pesticide pollution of surface water and wastewater has been recognized as a major worldwide concern due to their persistence in the aquatic environment and the potential adverse effects on human, flora, and fauna health. Apart from pesticides, bio-contamination with various bacterial populations leads to waterborne diseases. Hence, it becomes vital to remove the above-mentioned pollutants from water using a suitable process. Consequently, our study emphasized the potential benefits of a highly porous, chemically cross-linked 3D chitosan (CSGA) cryogel in the removal of pesticides and bacteria. The CSGA sponges were prepared using a facile and cost-effective approach that consisted of a three-step cryogenic process: (i) freezing at −18 °C, (ii) storage in a frozen state for a certain period, and (iii) thawing at room temperature. Batch adsorption experiments were performed under different environments, where the effects of several parameters, such as pH, contact time, and initial pollutant concentration were evaluated to identify the appropriate adsorption conditions for maximum pesticide removal. The CSGA-based cryogel sponges exhibited a theoretical maximum adsorption capacity of 160.82 mg g−1 for the Fastac 10EC pesticide and very good recyclability at room temperature. In addition, the antibacterial activities of these sponges were also investigated against various bacterial pathogens. The rates of killing Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus were close to 82%, 100%, and 99%, respectively. These results demonstrated that CSGA cryogels could be efficiently used in water remediation and find applications in the removal of pesticides and disinfection. Full article
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19 pages, 3225 KiB  
Article
Cytotoxicity Enhancement in MCF-7 Breast Cancer Cells with Depolymerized Chitosan Delivery of α-Mangostin
by Yedi Herdiana, Nasrul Wathoni, Shaharum Shamsuddin and Muchtaridi Muchtaridi
Polymers 2022, 14(15), 3139; https://doi.org/10.3390/polym14153139 - 01 Aug 2022
Cited by 9 | Viewed by 1722
Abstract
The application of α-mangostin (AMG) in breast cancer research has wide intentions. Chitosan-based nanoparticles (CSNPs) have attractive prospects for developing anticancer drugs, especially in their high flexibility for modification to enhance their anticancer action. This research aimed to study the impact of depolymerized [...] Read more.
The application of α-mangostin (AMG) in breast cancer research has wide intentions. Chitosan-based nanoparticles (CSNPs) have attractive prospects for developing anticancer drugs, especially in their high flexibility for modification to enhance their anticancer action. This research aimed to study the impact of depolymerized chitosan (CS) on the cytotoxicity enhancement of AMG in MCF-7 breast cancer cells. CSNPs effectivity depends on size, shape, crystallinity degree, and charge surface. Modifying CS molecular weight (MW) is expected to influence CSNPs’ characteristics, impacting size, shape, crystallinity degree, and charge surface. CSNPs are developed using the method of ionic gelation with sodium tripolyphosphate (TPP) as a crosslinker and spray pyrolysis procedure. Nanoparticles’ (NPs) sizes vary from 205.3 ± 81 nm to 450.9 ± 235 nm, ZP charges range from +10.56 mV to +51.56 mV, and entrapment efficiency from 85.35% to 90.45%. The morphology of NPs are all the same spherical forms. In vitro release studies confirmed that AMG–Chitosan–High Molecular Weight (AMG–CS–HMW) and AMG–Chitosan–Low Molecular Weight (AMG–CS–LMW) had a sustained-release system profile. MW has a great influence on surface, drug release, and cytotoxicity enhancement of AMG in CSNPs to MCF-7 cancer cells. The preparations AMG–CS–HMW and AMG–CS–LMW NPs considerably enhanced the cytotoxicity of MCF-7 cells with IC50 values of 5.90 ± 0.08 µg/mL and 4.90 ± 0.16 µg/mL, respectively, as compared with the non-nano particle formulation with an IC50 of 8.47 ± 0.29 µg/mL. These findings suggest that CSNPs can enhance the physicochemical characteristics and cytotoxicity of AMG in breast cancer treatment. Full article
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14 pages, 6479 KiB  
Article
Self-Healing Chitosan Hydrogels: Preparation and Rheological Characterization
by Anda Mihaela Craciun, Simona Morariu and Luminita Marin
Polymers 2022, 14(13), 2570; https://doi.org/10.3390/polym14132570 - 24 Jun 2022
Cited by 21 | Viewed by 3184
Abstract
The paper aims at the preparation of chitosan self-healing hydrogels, designed as carriers for local drug delivery by parenteral administration. To this aim, 30 hydrogels were prepared using chitosan and pyridoxal 5-phosphate (P5P), the active form of vitamin B6 as precursors, by varying [...] Read more.
The paper aims at the preparation of chitosan self-healing hydrogels, designed as carriers for local drug delivery by parenteral administration. To this aim, 30 hydrogels were prepared using chitosan and pyridoxal 5-phosphate (P5P), the active form of vitamin B6 as precursors, by varying the ratio of glucosamine units and aldehyde on the one hand and the water content on the other hand. The driving forces of hydrogelation were investigated by nuclear magnetic resonance (NMR), Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction, and polarized light microscopy (POM) measurements. NMR technique was also used to investigate the stability of hydrogels over time, and their morphological particularities were assessed by scanning electron microscopy (SEM). Degradability of the hydrogels was studied in media of four different pH, and preliminary self-healing ability was visually established by injection through a syringe needle. In-depth rheological investigation was conducted in order to monitor the storage and loss moduli, linear viscoelastic regime, and structural recovery capacity. It was concluded that chitosan crosslinking with pyridoxal 5-phosphate is a suitable route to reach self-healing hydrogels with a good balance of mechanical properties/structural recovery, good stability over time, and degradability controlled by pH. Full article
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18 pages, 3123 KiB  
Article
Lornoxicam-Loaded Chitosan-Decorated Nanoemulsion: Preparation and In Vitro Evaluation for Enhanced Transdermal Delivery
by Rahman Ullah Khan, Shefaat Ullah Shah, Sheikh Abdur Rashid, Faiza Naseem, Kifayat Ullah Shah, Arshad Farid, Khalid Rehman Hakeem, Majid Rasool Kamli, Eman Hillal Althubaiti and Soha A. Alamoudi
Polymers 2022, 14(9), 1922; https://doi.org/10.3390/polym14091922 - 09 May 2022
Cited by 11 | Viewed by 2171
Abstract
Nanoemulsions are promising drug delivery systems for the administration of poorly soluble drugs like lornoxicam (LRX) by oral or parenteral routes. Such formulations work perfectly for transdermal delivery of lornoxicam-type drugs. It has also been established that formulating such a delivery system is [...] Read more.
Nanoemulsions are promising drug delivery systems for the administration of poorly soluble drugs like lornoxicam (LRX) by oral or parenteral routes. Such formulations work perfectly for transdermal delivery of lornoxicam-type drugs. It has also been established that formulating such a delivery system is highly dependent on the presence, type, and concentration of excipients taking part in the formulation. The inherent characteristics of nanoemulsion (NE), i.e., smaller globule size and excipient nature, facilitate the drug’s passage through skin. The current study was aimed at the development of an NE-based formulation of LRX to improve the drug solubility in vitro as well as to enhance drug skin permeation to promote therapeutic outcome in appropriate time. Spontaneous self-emulsification technique was utilized to develop optimized LRX-encapsulated NE-based formulations. ATR-FTIR spectra of the pure drug and various formulations did not show any interaction between the drug and various formulation excipients showing compatibility. Globule size for stable formulations ranged between 63–168 nm. These formulations were characterized for viscosity, surface tension, pH, drug encapsulation efficiency, in vitro drug release, and drug skin permeation studies. Chitosan-decorated optimized NE formulation of LRX showed about 58.82% cumulative drug release, showing an anomalous non-Fickian diffusion mechanism of drug release. Drug encapsulation efficiency, in vitro drug release, and skin permeation studies exhibited promising results. An appreciable drug entrapment efficiency was exhibited by optimized NE formulations LRX-6, 71.91 ± 3.17% and C-LRX, 65.25 ± 4.89%. Permeability parameters like enhancement ratio (Er), permeability constant (Kp), and steady state flux (Jss) showed higher values and exhibited good results based on formulation type. The selected promising formulation type “LRX-6” showed significantly different results as compared to other formulations (LRX-4, 5, and 7). The skin permeation property of the LRX-6 formulation was compared to similar chitosan-based formulations and was found to have better skin permeation results than chitosan-based formulations. This study clearly exhibited that an LRX-containing NE-based formulation can be formulated to form a stable drug delivery system. Such formulations are promising in terms of physicochemical characteristics, improved solubility, and high skin permeation potential. Full article
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15 pages, 4018 KiB  
Article
Evaluating Non-Conventional Chitosan Sources for Controlled Release of Risperidone
by Sara Garcinuño, Inmaculada Aranaz, Concepción Civera, Concepción Arias and Niuris Acosta
Polymers 2022, 14(7), 1355; https://doi.org/10.3390/polym14071355 - 26 Mar 2022
Cited by 6 | Viewed by 1803
Abstract
In this work, two chitosan samples from cuttlebone and squid pen are produced and characterized. We studied the formation of thermoresponsive hydrogels with β-glycerol phosphate and found proper formulations that form the hydrogels at 37 °C. Gel formation depended on the chitosan source [...] Read more.
In this work, two chitosan samples from cuttlebone and squid pen are produced and characterized. We studied the formation of thermoresponsive hydrogels with β-glycerol phosphate and found proper formulations that form the hydrogels at 37 °C. Gel formation depended on the chitosan source being possible to produce the thermoresponsive hydrogels at chitosan concentration of 1% with cuttlebone chitosan but 1.5% was needed for squid pen. For the first time, these non-commercial chitosan sources have been used in combination with β-glycerol phosphate to prepare risperidone formulations for controlled drug delivery. Three types of formulations for risperidone-controlled release have been developed, in-situ gelling formulations, hydrogels and xerogels. The release profiles show that in-situ gelling formulations and particularly hydrogels allow an extended control release of risperidone while xerogels are not appropriate formulations for this end since risperidone was completely released in 48 h. Full article
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22 pages, 7405 KiB  
Article
Efficacy of Chitosan Nanoparticle Loaded-Salicylic Acid and -Silver on Management of Cassava Leaf Spot Disease
by Nguyen Huy Hoang, Toan Le Thanh, Wannaporn Thepbandit, Jongjit Treekoon, Chanon Saengchan, Rungthip Sangpueak, Narendra Kumar Papathoti, Anyanee Kamkaew and Natthiya Buensanteai
Polymers 2022, 14(4), 660; https://doi.org/10.3390/polym14040660 - 09 Feb 2022
Cited by 9 | Viewed by 2979
Abstract
Leaf spot is one of the most important cassava diseases. Nanotechnology can be applied to control diseases and improve plant growth. This study was performed to prepare chitosan (CS) nanoparticle (NP)-loaded salicylic acid (SA) or silver (Ag) by the ionic gelation method, and [...] Read more.
Leaf spot is one of the most important cassava diseases. Nanotechnology can be applied to control diseases and improve plant growth. This study was performed to prepare chitosan (CS) nanoparticle (NP)-loaded salicylic acid (SA) or silver (Ag) by the ionic gelation method, and to evaluate their effectiveness on reducing leaf spot disease and enhancing the growth of cassava plants. The CS (0.4 or 0.5%) and Pentasodium triphosphate (0.2 or 0.5%) were mixed with SA varying at 0.05, 0.1, or 0.2% or silver nitrate varying at 1, 2, or 3 mM to prepare three formulations of CS-NP-loaded SA named N1, N2, and N3 or CS-NP-loaded Ag named N4, N5, and N6. The results showed that the six formulations were not toxic to cassava leaves up to 800 ppm. The CS-NP-loaded SA (N3) and CS-NP-loaded Ag (N6) were more effective than the remaining formulations in reducing the disease severity and the disease index of leaf spot. Furthermore, N3 at 400 ppm and N6 at 200, 400, and 800 ppm could reduce disease severity (68.9–73.6% or 37.0–37.7%, depending on the time of treatment and the pathogen density) and enhance plant growth more than or equal to commercial fungicide or nano-fungicide products under net-house conditions. The study indicates the potential to use CS-NP-loaded SA or Ag as elicitors to manage cassava leaf spot disease. Full article
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15 pages, 4584 KiB  
Article
Biological Behavior of Xenogenic Scaffolds in Alcohol-Induced Rats: Histomorphometric and Picrosirius Red Staining Analysis
by Dayane Maria Braz Nogueira, André Luiz de Faria Figadoli, Patrícia Lopes Alcantara, Karina Torres Pomini, Iris Jasmin Santos German, Carlos Henrique Bertoni Reis, Geraldo Marco Rosa Júnior, Marcelie Priscila de Oliveira Rosso, Paulo Sérgio da Silva Santos, Mariana Schutzer Ragghianti Zangrando, Eliana de Souza Bastos Mazuqueli Pereira, Miguel Ângelo de Marchi, Beatriz Flavia de Moraes Trazzi, Jéssica de Oliveira Rossi, Samira Salmeron, Cláudio Maldonado Pastori, Daniela Vieira Buchaim and Rogerio Leone Buchaim
Polymers 2022, 14(3), 584; https://doi.org/10.3390/polym14030584 - 31 Jan 2022
Cited by 8 | Viewed by 2619
Abstract
In this experimental protocol, the objective was to evaluate the biological behavior of two xenogenic scaffolds in alcohol-induced rats through histomorphometric and Picrosirius Red staining analysis of non-critical defects in the tibia of rats submitted or not to alcohol ingestion at 25% v [...] Read more.
In this experimental protocol, the objective was to evaluate the biological behavior of two xenogenic scaffolds in alcohol-induced rats through histomorphometric and Picrosirius Red staining analysis of non-critical defects in the tibia of rats submitted or not to alcohol ingestion at 25% v/v. Eighty male rats were randomly divided into four groups (n = 20 each): CG/B (water diet + Bio-Oss® graft, Geistlich Pharma AG, Wolhusen, Switzerland), CG/O (water diet + OrthoGen® graft, Baumer, Mogi Mirim, Brazil), AG/B (25% v/v alcohol diet + Bio-Oss® graft), and AG/O (25% v/v alcohol diet + OrthoGen® graft). After 90 days of liquid diet, the rats were surgically obtained, with a defect in the tibia proximal epiphysis; filled in according to their respective groups; and euthanized at 10, 20, 40 and 60 days. In two initial periods (10 and 20 days), all groups presented biomaterial particles surrounded by disorganized collagen fibrils. Alcoholic animals (AG/B and AG/O) presented, in the cortical and medullary regions, a reactive tissue with inflammatory infiltrate. In 60 days, in the superficial area of the surgical cavities, particles of biomaterials were observed in all groups, with new compact bone tissue around them, without complete closure of the lesion, except in non-alcoholic animals treated with Bio-Oss® xenograft (CG/B), where the new cortical interconnected the edges of the defect. Birefringence transition was observed in the histochemical analysis of collagen fibers by Picrosirius Red, in which all groups in periods of 10 and 20 days showed red-orange birefringence, and from 40 days onwards greenish-yellow birefringence, which demonstrates the characteristic transition from the formation of thin and disorganized collagen fibers initially to more organized and thicker later. In histomorphometric analysis, at 60 days, CG/B had the highest volume density of new bone (32.9 ± 1.15) and AG/O the lowest volume density of new bone (15.32 ± 1.71). It can be concluded that the bone neoformation occurred in the defects that received the two biomaterials, in all periods, but the Bio-Oss® was superior in the results, with its groups CG/B and AG/B displaying greater bone formation (32.9 ± 1.15 and 22.74 ± 1.15, respectively) compared to the OrthoGen® CG/O and AG/O groups (20.66 ± 2.12 and 15.32 ± 1.71, respectively), and that the alcoholic diet interfered negatively in the repair process and in the percentage of new bone formed. Full article
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15 pages, 2463 KiB  
Article
Adsorption of Cu (II) Ions Present in the Distilled Beverage (Sugar Cane Spirit) Using Chitosan Derived from the Shrimp Shell
by Lucely Nogueira dos Santos, Alberdan Silva Santos, Kelly das Graças Fernandes Dantas and Nelson Rosa Ferreira
Polymers 2022, 14(3), 573; https://doi.org/10.3390/polym14030573 - 31 Jan 2022
Cited by 5 | Viewed by 2627
Abstract
Cachaça (sugar cane spirit) is a typically Brazilian distilled beverage. Copper ions can be present in craft beverages despite their acceptance in the national and international market. This study aims to evaluate the efficiency of chitosan as an adsorbent in removing copper (II) [...] Read more.
Cachaça (sugar cane spirit) is a typically Brazilian distilled beverage. Copper ions can be present in craft beverages despite their acceptance in the national and international market. This study aims to evaluate the efficiency of chitosan as an adsorbent in removing copper (II) from cachaça. The structural characteristics of the obtained chitosan and the effect of adsorbed copper were evaluated by Fourier Transform Infrared Spectroscopy (ATR-FTIR), viscosimetry, X-ray diffraction (XRD), and scanning electron microscopy (SEM). The deacetylation reaction from chitin (shrimp shell) resulted in chitosan with a deacetylation degree of 88.9% (potentiometric titration) and 86.9% (FTIR), low crystallinity, and an estimated molecular weight of 162.96 kDa. The copper reduction rate was 84.09% evaluated by spectrophotometric titration and microwave-induced plasma optical emission spectrometry (MIP–OES). The amine groups of chitosan had adsorption affinity with copper ions, and the kinetic analysis showed a better fit of the data by the Elovich equation, suggesting that the chemosorption mechanism controlled the kinetic process. The results suggest that chitosan has the potential to improve the quality and safety of cachaça. Full article
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15 pages, 4620 KiB  
Article
Drug Delivery from Stimuli-Responsive Poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide)/Chitosan Core/Shell Nanohydrogels
by Andrés Ortega-García, Bryan Giovanny Martínez-Bernal, Israel Ceja, Eduardo Mendizábal, Jorge Emilio Puig-Arévalo and Lourdes Adriana Pérez-Carrillo
Polymers 2022, 14(3), 522; https://doi.org/10.3390/polym14030522 - 27 Jan 2022
Cited by 6 | Viewed by 2562
Abstract
The synthesis of stimulus-responsive poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide)/chitosan core/shell nanohydrogels made by batch emulsion polymerization in the presence of chitosan (CS) micelles is reported. The ratio of monomers required to obtain copolymers with a volume phase transition temperature (TVPT) in the range of the [...] Read more.
The synthesis of stimulus-responsive poly(N-isopropylacrylamide-co-N-isopropylmethacrylamide)/chitosan core/shell nanohydrogels made by batch emulsion polymerization in the presence of chitosan (CS) micelles is reported. The ratio of monomers required to obtain copolymers with a volume phase transition temperature (TVPT) in the range of the temperatures observed in the human body in response to an infection (38 to 40 °C) was estimated with the Fox equation. The conversion was determined by gravimetry; mean particle size, size distribution, and thermal response were measured by quasi-elastic light scattering (QLS). The core/shell structure was confirmed by TEM, and FTIR showed the presence of N-isopropyl acrilamide (NIPA), N-isopropyl methacrylamide (NIPMA), and CS in the nanohydrogels. The nanohydrogels were loaded with the drug doxycycline hyclate, and their release kinetic profile was determined at pH = 2.0 and 7.4 at their volume phase transition temperatures (TVPT). A higher amount of drug was released at acidic pH. Some mathematical models described in the literature were used to fit the experimental drug release data. Full article
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16 pages, 4762 KiB  
Article
In Vivo Study of Nasal Bone Reconstruction with Collagen, Elastin and Chitosan Membranes in Abstainer and Alcoholic Rats
by Fabricio Egidio Pandini, Fabíola Mayumi Miyauchi Kubo, Ana Maria de Guzzi Plepis, Virginia da Conceição Amaro Martins, Marcelo Rodrigues da Cunha, Vinicius Rodrigues Silva, Vinicius Barroso Hirota, Everton Lopes, Marcos Antonio Menezes, André Antonio Pelegrine, Tiago Negrão de Andrade, Amilton Iatecola, Bruna da Cruz Britto, Victor Augusto Ramos Fernandes, Luis Felipe Orsi Gameiro, Ronny Rodrigues Correia, Marcelo Lucchesi Teixeira, Getúlio Duarte Júnior, Carlos Henrique Bertoni Reis, Eliana de Souza Bastos Mazuqueli Pereira, Daniela Vieira Buchaim, Karina Torres Pomini, Daniel de Bortoli Teixeira, Rogerio Leone Buchaim and Edmir Américo Lourençoadd Show full author list remove Hide full author list
Polymers 2022, 14(1), 188; https://doi.org/10.3390/polym14010188 - 04 Jan 2022
Cited by 11 | Viewed by 2310
Abstract
The aim of the present study was to evaluate the use of collagen, elastin, or chitosan biomaterial for bone reconstruction in rats submitted or not to experimental alcoholism. Wistar male rats were divided into eight groups, submitted to chronic alcohol ingestion (G5 to [...] Read more.
The aim of the present study was to evaluate the use of collagen, elastin, or chitosan biomaterial for bone reconstruction in rats submitted or not to experimental alcoholism. Wistar male rats were divided into eight groups, submitted to chronic alcohol ingestion (G5 to G8) or not (G1 to G4). Nasal bone defects were filled with clot in animals of G1 and G5 and with collagen, elastin, and chitosan grafts in G2/G6, G3/G7, and G4/G8, respectively. Six weeks after, all specimens underwent radiographic, tomographic, and microscopic evaluations. Bone mineral density was lower in the defect area in alcoholic animals compared to the abstainer animals. Bone neoformation was greater in the abstainer groups receiving the elastin membrane and in abstainer and alcoholic rats receiving the chitosan membrane (15.78 ± 1.19, 27.81 ± 0.91, 47.29 ± 0.97, 42.69 ± 1.52, 13.81 ± 1.60, 18.59 ± 1.37, 16.54 ± 0.89, and 37.06 ± 1.17 in G1 to G8, respectively). In conclusion, osteogenesis and bone density were more expressive after the application of the elastin matrix in abstainer animals and of the chitosan matrix in both abstainer and alcoholic animals. Chronic alcohol ingestion resulted in lower bone formation and greater formation of fibrous connective tissue. Full article
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2021

Jump to: 2023, 2022, 2020

24 pages, 4524 KiB  
Article
Assessment of Antifungal Efficacy and Release Behavior of Fungicide-Loaded Chitosan-Carrageenan Nanoparticles against Phytopathogenic Fungi
by Ravinder Kumar, Agnieszka Najda, Joginder Singh Duhan, Balvinder Kumar, Prince Chawla, Joanna Klepacka, Seweryn Malawski, Pardeep Kumar Sadh and Anil Kumar Poonia
Polymers 2022, 14(1), 41; https://doi.org/10.3390/polym14010041 - 23 Dec 2021
Cited by 16 | Viewed by 3381
Abstract
Biopolymeric Chitosan-Carrageenan nanocomposites 66.6–231.82 nm in size containing the chemical fungicide mancozeb (nano CSCRG-M) were synthesized following a green chemistry approach. The physicochemical study of nanoparticles (NPs) was accomplished using a particle size analyzer, SEM and FTIR. TEM exhibited clover leaf-shaped nanoparticles (248.23 [...] Read more.
Biopolymeric Chitosan-Carrageenan nanocomposites 66.6–231.82 nm in size containing the chemical fungicide mancozeb (nano CSCRG-M) were synthesized following a green chemistry approach. The physicochemical study of nanoparticles (NPs) was accomplished using a particle size analyzer, SEM and FTIR. TEM exhibited clover leaf-shaped nanoparticles (248.23 nm) with mancozeb on the inside and entrapped outside. Differential scanning calorimetry and TGA thermogravimetry exhibited the thermal behaviour of the nanoform. Nano CSCRG-1.5 at 1.5 ppm exhibited 83.1% inhibition against Alternaria solani in an in vitro study and performed as well as mancozeb (84.6%). Complete inhibition was exhibited in Sclerotinia sclerotiorum at 1.0 and 1.5 ppm with the nanoformulation. The in vivo disease control efficacy of mancozeb-loaded nanoparticles against A. solani in pathogenized plants was found to be relatively higher (79.4 ± 1.7) than that of commercial fungicide (76 ± 1.1%) in pot conditions. Nanomancozeb showed superior efficacy for plant growth parameters, such as germination percentage, root–shoot ratio and dry biomass. The nanoformulation showed higher cell viability compared to mancozeb in Vero cell cultures at 0.25 and 0.50 mg/mL in the resazurin assay. CSCRG-0.5 showed slow-release behavior up to 10 h. Thus, these green nano-based approaches may help combat soil and water pollution caused by harmful chemical pesticides. Full article
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18 pages, 2218 KiB  
Review
Optimization of Chitosan Properties with the Aim of a Water Resistant Adhesive Development
by Jeanne Silvestre, Cédric Delattre, Philippe Michaud and Hélène de Baynast
Polymers 2021, 13(22), 4031; https://doi.org/10.3390/polym13224031 - 21 Nov 2021
Cited by 18 | Viewed by 3683
Abstract
Chitosan is a bio-sourced polysaccharide widely used in different fields from health to wastewater treatment through food supplements. Another important use of this polymer is adhesion. Indeed, the current demand to replace non-natural and hazardous polymers by greener ones is well present in [...] Read more.
Chitosan is a bio-sourced polysaccharide widely used in different fields from health to wastewater treatment through food supplements. Another important use of this polymer is adhesion. Indeed, the current demand to replace non-natural and hazardous polymers by greener ones is well present in the adhesive field and open good opportunities for chitosan and its derivatives. However, chitosan is water soluble and exhibits a poor water-resistance in the field of adhesion which reduces the possibilities of its utilization within the paste field. This review focuses on exploration of different ways available to modify the chitosan and transform it into a water-resistant adhesive. The first part concerns the chitosan itself and gives important information from the discovery of chitin to the pure chitosan ready to use. The second part reviews the background information relative to adhesion theories, ideal properties of adhesives and the characteristics of chitosan as an adhesive. The last part focuses on exploration of the possible modification of chitosan to make it a water-resistant chemical adhesive. Full article
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15 pages, 3629 KiB  
Article
Synergistic Effect of Glycyrrhizic Acid and ZnO/Palygorskite on Improving Chitosan-Based Films and Their Potential Application in Wound Healing
by Qian Zhang, Hong Zhang, Aiping Hui, Junjie Ding, Xinyue Liu and Aiqin Wang
Polymers 2021, 13(22), 3878; https://doi.org/10.3390/polym13223878 - 10 Nov 2021
Cited by 8 | Viewed by 2140
Abstract
The synergistic effect of chitosan (CS), glycyrrhizic acid (GA) and ZnO/palygorskite (ZnO/PAL) as potential wound dressing was evaluated in the form of films by the solution casting method. The nanocomposite films were well-characterized with ATR-FTIR, XRD and SEM to explore the interactions between [...] Read more.
The synergistic effect of chitosan (CS), glycyrrhizic acid (GA) and ZnO/palygorskite (ZnO/PAL) as potential wound dressing was evaluated in the form of films by the solution casting method. The nanocomposite films were well-characterized with ATR-FTIR, XRD and SEM to explore the interactions between CS, GA and ZnO/PAL. Physical, mechanical and antibacterial properties of the nanocomposite films were systematically investigated for their reliability in end-up utilization. Importantly, it was found that the presence of PAL in the films provided enhanced mechanical properties, whereas CS, GA and ZnO supplied a broad-spectrum antibacterial activity, especially for drug-resistant bacteria such as ESBL—E. coli and MRSA. Overall, this research demonstrated that the prepared films can be a promising candidate for wound-care materials. Full article
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21 pages, 10251 KiB  
Article
Chitosan/Polyvinyl Alcohol/Tea Tree Essential Oil Composite Films for Biomedical Applications
by Jorge Iván Castro, Carlos Humberto Valencia-Llano, Mayra Eliana Valencia Zapata, Yilmar Joan Restrepo, José Herminsul Mina Hernandez, Diana Paola Navia-Porras, Yamid Valencia, Cesar Valencia and Carlos David Grande-Tovar
Polymers 2021, 13(21), 3753; https://doi.org/10.3390/polym13213753 - 29 Oct 2021
Cited by 20 | Viewed by 3184
Abstract
Tissue engineering is crucial, since its early adoption focused on designing biocompatible materials that stimulate cell adhesion and proliferation. In this sense, scaffolds made of biocompatible and resistant materials became the researchers’ focus on biomedical applications. Humans have used essential oils for a [...] Read more.
Tissue engineering is crucial, since its early adoption focused on designing biocompatible materials that stimulate cell adhesion and proliferation. In this sense, scaffolds made of biocompatible and resistant materials became the researchers’ focus on biomedical applications. Humans have used essential oils for a long time to take advantage of their antifungal, insecticide, antibacterial, and antioxidant properties. However, the literature demonstrating the use of essential oils for stimulating biocompatibility in new scaffold designs is scarce. For that reason, this work describes the synthesis of four different film composites of chitosan/polyvinyl alcohol/tea tree (Melaleuca alternifolia), essential oil (CS/PVA/TTEO), and the subdermal implantations after 90 days in Wistar rats. According to the Young modulus, DSC, TGA, mechanical studies, and thermal studies, there was a reinforcement effect with the addition of TTEO. Morphology and energy-dispersive (EDX) analysis after the immersion in simulated body fluid (SBF) exhibited a light layer of calcium chloride and sodium chloride generated on the material’s surface, which is generally related to a bioactive material. Finally, the biocompatibility of the films was comparable with porcine collagen, showing better signs of resorption as the amount of TTEO was increased. These results indicate the potential application of the films in long-term biomedical needs. Full article
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12 pages, 766 KiB  
Article
A Study of Compressibility, Compactability and Mucoadhesivity of Tableting Materials for Matrix Systems Based on Chitosan
by Jitka Muzikova, Eva Snejdrova, Juraj Martiska, Bara Doubkova and Andrea Veris
Polymers 2021, 13(21), 3636; https://doi.org/10.3390/polym13213636 - 21 Oct 2021
Cited by 1 | Viewed by 1673
Abstract
The objective of the present research is to evaluate directly compressible chitosan-based tableting materials for the formulation of mucoadhesive matrix tablets intended for targeted drug release to distal segments of the GIT. The influence of sodium alginate, hypromellose, and silicified microcrystalline cellulose (P90) [...] Read more.
The objective of the present research is to evaluate directly compressible chitosan-based tableting materials for the formulation of mucoadhesive matrix tablets intended for targeted drug release to distal segments of the GIT. The influence of sodium alginate, hypromellose, and silicified microcrystalline cellulose (P90) on compressibility, compactability and lubricant sensitivity ratio was tested. Furthermore, the rheological properties of the hydrated surface layer of the matrix tablets and the mucoadhesion to a mucin substrate were analysed. Compressibility was evaluated using the energy profile of the compression process, compactability by means of the tensile strength of tablets, and lubricant sensitivity ratio was calculated to assess the sensitivity to lubricant. Addition of P90 to chitosan improved compressibility, which is demonstrated by the increase in the energy of plastic deformation and the higher tensile strength of tablets. P90 also significantly reduced the high lubricant sensitivity of chitosan. Presence of retarding components led to a decrease in Emax. All tested matrix tablets revealed a good mucoadhesion without a negative effect of P90 content. The viscosity of a gel layer on the surface of matrix tablets containing hypromellose was higher compared to those with sodium alginate. This was not reflected in the adhesive strength of the tablets. The formulated tableting materials combining chitosan and P90 are a suitable matrix for incorporation of an active ingredient, whose delayed release in the intestine can be achieved by the functionality of the chitosan-sodium alginate complex. Full article
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17 pages, 2947 KiB  
Article
An Engineered Specificity of Anti-Neoplastic Agent Loaded Magnetic Nanoparticles for the Treatment of Breast Cancer
by Anroop B. Nair, Mallikarjun Telsang and Riyaz Ali Osmani
Polymers 2021, 13(21), 3623; https://doi.org/10.3390/polym13213623 - 20 Oct 2021
Cited by 3 | Viewed by 1565
Abstract
Nanoparticles have gained increased attention due to the prospection of drug delivery at target sites, thus limiting the systemic effects of the drugs. Their efficiency was further improved by adding special carriers such as magnetite (Fe3O4). It is one [...] Read more.
Nanoparticles have gained increased attention due to the prospection of drug delivery at target sites, thus limiting the systemic effects of the drugs. Their efficiency was further improved by adding special carriers such as magnetite (Fe3O4). It is one of the extensively used oxides of iron for both pharmaceutical and biomedical applications owing to its ease of preparation and biocompatibility. In this work, Gemcitabine magnetic nanoparticles were prepared using Fe3O4 and chitosan as the primary ingredients. Optimization was accomplished by Box–Behnken Design and factor interactions were evaluated. The desirability function approach was made to enhance the formulation concerning particle size, polydispersity index, and zeta potential. Based on this, optimized magnetic nanoparticles (O-MNP) were formulated with 300 mg of Fe3O4, 297.7 mg of chitosan, and a sonication time of 2.4 h, which can achieve the prerequisites of the target formulation. All other in vitro parameters were found to be following the requirement. In vitro cytotoxic studies for O-MNP were performed using cell cultures of breast cancer (MCF-7), leukemia (THP-1), prostate cancer (PC-3), and lung cancer (A549). O-MNP showed maximum inhibition growth with MCF-7 cell lines rather than other cell lines. The data observed here demonstrates the potential of magnetic nanoparticles of gemcitabine in treating breast cancers. Full article
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17 pages, 4678 KiB  
Article
Development of pH-Sensitive Chitosan-g-poly(acrylamide-co-acrylic acid) Hydrogel for Controlled Drug Delivery of Tenofovir Disoproxil Fumarate
by Justin B. Safari, Alain M. Bapolisi and Rui W. M. Krause
Polymers 2021, 13(20), 3571; https://doi.org/10.3390/polym13203571 - 16 Oct 2021
Cited by 12 | Viewed by 3179
Abstract
The present study aimed to develop a pH-sensitive chitosan-based hydrogel for controlled delivery of an anti-hepatitis B drug, tenofovir disoproxil fumarate (TDF). Free radical polymerization was utilized to graft acrylamide and acrylic acid using N,N-methylene bisacrylamide as the crosslinker. Physicochemical [...] Read more.
The present study aimed to develop a pH-sensitive chitosan-based hydrogel for controlled delivery of an anti-hepatitis B drug, tenofovir disoproxil fumarate (TDF). Free radical polymerization was utilized to graft acrylamide and acrylic acid using N,N-methylene bisacrylamide as the crosslinker. Physicochemical characterization confirmed the synthesis of thermally stable chitosan-g-poly(acrylamide-co-acrylic acid) hydrogels with well-defined pores within a fibrous surface. The prepared hydrogels exhibited pH and ionic strength sensitivity, with the swelling significantly lower under acidic and strong ionic strength conditions but higher in neutral and basic solutions. In addition, cytotoxicity studies on HeLa cell lines proved the cytocompatibility of the drug delivery material and its readiness for physiological applications. The encapsulation of TDF in the hydrogels was optimized and an encapsulation efficiency and a drug loading percentage of 96% and 10% were achieved, respectively. More interestingly, in vitro release studies demonstrated a pH-dependent release of TDF from hydrogels. The release at pH 7.4 was found to be up to five times higher than at pH 1.2 within 96 h. This further suggested that the newly developed hydrogel-loaded TDF could be proposed as a smart delivery system for oral delivery of anti-hepatitis B drugs. Full article
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16 pages, 17198 KiB  
Article
The Effect of Biopolymer Chitosan on the Rheology and Stability of Na-Bentonite Drilling Mud
by Basim Abu-Jdayil, Mamdouh Ghannam, Karam Alsayyed Ahmed and Mohamed Djama
Polymers 2021, 13(19), 3361; https://doi.org/10.3390/polym13193361 - 30 Sep 2021
Cited by 9 | Viewed by 2593
Abstract
The utilization of greens resources is a grand challenge for this century. A lot of efforts are paid to substitute toxic ingredients of the conventional drilling mud system with nontoxic natural materials. In this paper, the effect of the natural polymer chitosan on [...] Read more.
The utilization of greens resources is a grand challenge for this century. A lot of efforts are paid to substitute toxic ingredients of the conventional drilling mud system with nontoxic natural materials. In this paper, the effect of the natural polymer chitosan on the rheology and stability of sodium-bentonite drilling mud was investigated in the polymer concentration range of 0.1–3.0 wt.%. Both the shear and time dependent rheological properties of pure chitosan, pure bentonite and bentonite–chitosan dispersions were studied. Moreover, zeta potential measurements were used to evaluate the stability of bentonite-chitosan suspension. Adding chitosan improved the natural properties of drilling mud, namely: yield stress, shear thinning, and thixotropy. The viscosity of bentonite suspension increased significantly upon the addition of chitosan in the concentration range of 0.5 to 3.0 wt.% forming network structure, which can be attributed to the interactions of hydrogen bonding between -OH clusters on the bentonite surface with the NH group in the chitosan structure. On the other hand, dispersed chitosan–bentonite suspension was observed at low chitosan concentration (less than 0.5 wt.%). Increasing both bentonite and chitosan concentrations led to the flocculation of the bentonite suspension, forming a continuous gel structure that was characterized by noteworthy yield stress. The desired drilling mud rheological behavior can be obtained with less bentonite by adding chitosan polymer and the undesirable effects of high solid clay concentration can be avoided. Full article
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15 pages, 3164 KiB  
Article
Chitosan-Coated 5-Fluorouracil Incorporated Emulsions as Transdermal Drug Delivery Matrices
by Taif Ali Khan, Abul Kalam Azad, Shivkanya Fuloria, Asif Nawaz, Vetriselvan Subramaniyan, Muhammad Akhlaq, Muhammad Safdar, Kathiresan V. Sathasivam, Mahendran Sekar, Omji Porwal, Dhanalekshmi Unnikrishnan Meenakshi, Rishabha Malviya, Mireia Mallandrich Miret, Ajay Mendiratta and Neeraj Kumar Fuloria
Polymers 2021, 13(19), 3345; https://doi.org/10.3390/polym13193345 - 29 Sep 2021
Cited by 27 | Viewed by 2671
Abstract
The purpose of the present study was to develop emulsions encapsulated by chitosan on the outer surface of a nano droplet containing 5-fluorouracil (5-FU) as a model drug. The emulsions were characterized in terms of size, pH and viscosity and were evaluated for [...] Read more.
The purpose of the present study was to develop emulsions encapsulated by chitosan on the outer surface of a nano droplet containing 5-fluorouracil (5-FU) as a model drug. The emulsions were characterized in terms of size, pH and viscosity and were evaluated for their physicochemical properties such as drug release and skin permeation in vitro. The emulsions containing tween 80 (T80), sodium lauryl sulfate, span 20, and a combination of polyethylene glycol (PEG) and T20 exhibited a release of 88%, 86%, 90% and 92%, respectively. Chitosan-modified emulsions considerably controlled the release of 5-FU compared to a 5-FU solution (p < 0.05). All the formulations enabled transportation of 5-FU through a rat’s skin. The combination (T80, PEG) formulation showed a good penetration profile. Different surfactants showed variable degrees of skin drug retention. The ATR-FTIR spectrograms revealed that the emulsions mainly affected the fluidization of lipids and proteins of the stratum corneum (SC) that lead to enhanced drug permeation and retention across the skin. The present study concludes that the emulsions containing a combination of surfactants (Tween) and a co-surfactant (PEG) exhibited the best penetration profile, prevented the premature release of drugs from the nano droplet, enhanced the permeation and the retention of the drug across the skin and had great potential for transdermal drug delivery. Therefore, chitosan-coated 5-FU emulsions represent an excellent possibility to deliver a model drug as a transdermal delivery system. Full article
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13 pages, 4590 KiB  
Article
Catalyst-Free Crosslinking Modification of Nata-de-Coco-Based Bacterial Cellulose Nanofibres Using Citric Acid for Biomedical Applications
by Rabiu Salihu, Mohamed Nainar Mohamed Ansari, Saiful Izwan Abd Razak, Nurliyana Ahmad Zawawi, Shafinaz Shahir, Mohd Helmi Sani, Muhammad Hanif Ramlee, Mohammed Ahmad Wsoo, Abdul Halim Mohd Yusof, Nadirul Hasraf Mat Nayan and Ahmad Mohammed Gumel
Polymers 2021, 13(17), 2966; https://doi.org/10.3390/polym13172966 - 31 Aug 2021
Cited by 3 | Viewed by 3359
Abstract
Bacterial cellulose (BC) has gained attention among researchers in materials science and bio-medicine due to its fascinating properties. However, BC’s fibre collapse phenomenon (i.e., its inability to reabsorb water after dehydration) is one of the drawbacks that limit its potential. To overcome this, [...] Read more.
Bacterial cellulose (BC) has gained attention among researchers in materials science and bio-medicine due to its fascinating properties. However, BC’s fibre collapse phenomenon (i.e., its inability to reabsorb water after dehydration) is one of the drawbacks that limit its potential. To overcome this, a catalyst-free thermal crosslinking reaction was employed to modify BC using citric acid (CA) without compromising its biocompatibility. FTIR, XRD, SEM/EDX, TGA, and tensile analysis were carried out to evaluate the properties of the modified BC (MBC). The results confirm the fibre crosslinking phenomenon and the improvement of some properties that could be advantageous for various applications. The modified nanofibre displayed an improved crystallinity and thermal stability with increased water absorption/swelling and tensile modulus. The MBC reported here can be used for wound dressings and tissue scaffolding. Full article
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20 pages, 60982 KiB  
Article
Synthesis and Characterization of Porous, Electro-Conductive Chitosan–Gelatin–Agar-Based PEDOT: PSS Scaffolds for Potential Use in Tissue Engineering
by Dania Adila Ahmad Ruzaidi, Mohd Muzamir Mahat, Zarif Mohamed Sofian, Nikman Adli Nor Hashim, Hazwanee Osman, Mohd Azizi Nawawi, Rosmamuhamadani Ramli, Khairil Anuar Jantan, Muhammad Faiz Aizamddin, Hazeeq Hazwan Azman, Yee Hui Robin Chang and Hairul Hisham Hamzah
Polymers 2021, 13(17), 2901; https://doi.org/10.3390/polym13172901 - 28 Aug 2021
Cited by 20 | Viewed by 5427
Abstract
Herein we report the synthesis and characterization of electro-conductive chitosan–gelatin–agar (Cs-Gel-Agar) based PEDOT: PSS hydrogels for tissue engineering. Cs-Gel-Agar porous hydrogels with 0–2.0% (v/v) PEDOT: PSS were fabricated using a thermal reverse casting method where low melting agarose served [...] Read more.
Herein we report the synthesis and characterization of electro-conductive chitosan–gelatin–agar (Cs-Gel-Agar) based PEDOT: PSS hydrogels for tissue engineering. Cs-Gel-Agar porous hydrogels with 0–2.0% (v/v) PEDOT: PSS were fabricated using a thermal reverse casting method where low melting agarose served as the pore template. Sample characterizations were performed by means of scanning electron microscopy (SEM), attenuated total reflectance–Fourier transform infrared spectroscopy (ATR–FTIR), X-ray diffraction analysis (XRD) and electrochemical impedance spectroscopy (EIS). Our results showed enhanced electrical conductivity of the cs-gel-agar hydrogels when mixed with DMSO-doped PEDOT: PSS wherein the optimum mixing ratio was observed at 1% (v/v) with a conductivity value of 3.35 × 10−4 S cm−1. However, increasing the PEDOT: PSS content up to 1.5 % (v/v) resulted in reduced conductivity to 3.28 × 10−4 S cm−1. We conducted in vitro stability tests on the porous hydrogels using phosphate-buffered saline (PBS) solution and investigated the hydrogels’ performances through physical observations and ATR–FTIR characterization. The present study provides promising preliminary data on the potential use of Cs-Gel-Agar-based PEDOT: PSS hydrogel for tissue engineering, and these, hence, warrant further investigation to assess their capability as biocompatible scaffolds. Full article
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15 pages, 3197 KiB  
Article
Study of Different Chitosan/Sodium Carboxymethyl Cellulose Proportions in the Development of Polyelectrolyte Complexes for the Sustained Release of Clarithromycin from Matrix Tablets
by Víctor Guarnizo-Herrero, Carlos Torrado-Salmerón, Norma Sofía Torres Pabón, Guillermo Torrado Durán, Javier Morales and Santiago Torrado-Santiago
Polymers 2021, 13(16), 2813; https://doi.org/10.3390/polym13162813 - 21 Aug 2021
Cited by 16 | Viewed by 3039
Abstract
This study investigated the combination of different proportions of cationic chitosan and anionic carboxymethyl cellulose (CMC) for the development of polyelectrolyte complexes to be used as a carrier in a sustained-release system. Analysis via scanning electron microscopy (SEM) Fourier transform infrared spectroscopy (FTIR), [...] Read more.
This study investigated the combination of different proportions of cationic chitosan and anionic carboxymethyl cellulose (CMC) for the development of polyelectrolyte complexes to be used as a carrier in a sustained-release system. Analysis via scanning electron microscopy (SEM) Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), and powder X-ray diffraction (PXRD) confirmed ionic interactions occur between the chitosan and carboxymethyl cellulose chains, which increases drug entrapment. The results of the dissolution study in acetate buffer (pH 4.2) showed significant increases in the kinetic profiles of clarithromycin for low proportions of chitosan/carboxymethyl cellulose tablets, while the tablets containing only chitosan had high relaxation of chitosan chains and disintegrated rapidly. The Korsmeyer–Peppas kinetic model for the different interpolymer complexes demonstrated that the clarithromycin transport mechanism was controlled by Fickian diffusion. These results suggest that the matrix tablets with different proportions of chitosan/carboxymethyl cellulose enhanced the ionic interaction and enabled the prolonged release of clarithromycin. Full article
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16 pages, 5116 KiB  
Article
Chitosan-Coated Poly(lactic acid) Nanofibres Loaded with Essential Oils for Wound Healing
by Giulia Milanesi, Barbara Vigani, Silvia Rossi, Giuseppina Sandri and Elisa Mele
Polymers 2021, 13(16), 2582; https://doi.org/10.3390/polym13162582 - 04 Aug 2021
Cited by 23 | Viewed by 3287
Abstract
Chronic skin wounds are characterised by a non-healing process that makes necessary the application of wound dressings on the damaged area to promote and facilitate the recovery of skin’s physiological integrity. The aim of the present work is to develop a bioactive dressing [...] Read more.
Chronic skin wounds are characterised by a non-healing process that makes necessary the application of wound dressings on the damaged area to promote and facilitate the recovery of skin’s physiological integrity. The aim of the present work is to develop a bioactive dressing that, once applied on the injured tissue, would exert antibacterial activity and promote adhesion and proliferation of fibroblasts. Nanofibres consisting of poly(lactic acid) (PLA) and essential oils (EOs) were electrospun and coated with a medium molecular weight chitosan (CS). Black pepper essential oil (BP-EO) or limonene (L), well-known for their antibacterial properties, were added to the PLA/acetone solution before electrospinning; phase separation phenomena occurred due to the poor solubility of the EOs in the PLA solution and led to fibres having surface nano-pores. The porous electrospun fibres were coated with CS to produce hydrophilic membranes that were easy to handle, biocompatible, and suited to promote cellular proliferation. The fibrous scaffolds were tested in terms of mechanical resistance, wettability, antibacterial activity, in-vitro cytotoxicity, and ability to promote fibroblasts’ adhesion and proliferation. The results obtained proved that the CS coating improved the hydrophilicity of the fibrous mats, enhanced EO’s antibacterial potential, and promoted cell adhesion and proliferation. Full article
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31 pages, 4931 KiB  
Review
Biomedical Applications of Quaternized Chitosan
by Kamla Pathak, Shashi Kiran Misra, Aayush Sehgal, Sukhbir Singh, Simona Bungau, Agnieszka Najda, Robert Gruszecki and Tapan Behl
Polymers 2021, 13(15), 2514; https://doi.org/10.3390/polym13152514 - 30 Jul 2021
Cited by 50 | Viewed by 5800
Abstract
The natural polymer chitosan is the second most abundant biopolymer on earth after chitin and has been extensively explored for preparation of versatile drug delivery systems. The presence of two distinct reactive functional groups (an amino group at C2, and a primary and [...] Read more.
The natural polymer chitosan is the second most abundant biopolymer on earth after chitin and has been extensively explored for preparation of versatile drug delivery systems. The presence of two distinct reactive functional groups (an amino group at C2, and a primary and secondary hydroxyl group at C3 and C6) of chitosan are involved in the transformation of expedient derivatives such as acylated, alkylated, carboxylated, quaternized and esterified chitosan. Amongst these, quaternized chitosan is preferred in pharmaceutical industries owing to its prominent features including superior water solubility, augmented antimicrobial actions, modified wound healing, pH-sensitive targeting, biocompatibility, and biodegradability. It has been explored in a large realm of pharmaceuticals, cosmeceuticals, and the biomedical arena. Immense classy drug delivery systems containing quaternized chitosan have been intended for tissue engineering, wound healing, gene, and vaccine delivery. This review article outlines synthetic techniques, basic characteristics, inherent properties, biomedical applications, and ubiquitous challenges associated to quaternized chitosan. Full article
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20 pages, 3839 KiB  
Article
Formulation and Antibacterial Activity Evaluation of Quaternized Aminochitosan Membrane for Wound Dressing Applications
by Ahmed M. Omer, Tamer M. Tamer, Randa E. Khalifa, Abdelazeem S. Eltaweil, Mona M. Agwa, Sally Sabra, Mahmoud S. Abd-Elmonem, Mohamed S. Mohy-Eldin and Zyta M. Ziora
Polymers 2021, 13(15), 2428; https://doi.org/10.3390/polym13152428 - 23 Jul 2021
Cited by 21 | Viewed by 2515
Abstract
Much attention has been paid to chitosan biopolymer for advanced wound dressing owing to its exceptional biological characteristics comprising biodegradability, biocompatibility and respectable antibacterial activity. This study intended to develop a new antibacterial membrane based on quaternized aminochitosan (QAMCS) derivative. Herein, aminochitosan (AMCS) [...] Read more.
Much attention has been paid to chitosan biopolymer for advanced wound dressing owing to its exceptional biological characteristics comprising biodegradability, biocompatibility and respectable antibacterial activity. This study intended to develop a new antibacterial membrane based on quaternized aminochitosan (QAMCS) derivative. Herein, aminochitosan (AMCS) derivative was quaternized by N-(2-Chloroethyl) dimethylamine hydrochloride with different ratios. The pre-fabricated membranes were characterized by several analysis tools. The results indicate that maximum surface potential of +42.2 mV was attained by QAMCS3 membrane compared with +33.6 mV for native AMCS membrane. Moreover, membranes displayed higher surface roughness (1.27 ± 0.24 μm) and higher water uptake value (237 ± 8%) for QAMCS3 compared with 0.81 ± 0.08 μm and 165 ± 6% for neat AMCS membranes. Furthermore, the antibacterial activities were evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus cereus. Superior antibacterial activities with maximum inhibition values of 80–98% were accomplished by QAMCS3 membranes compared with 57–72% for AMCS membrane. Minimum inhibition concentration (MIC) results denote that the antibacterial activities were significantly boosted with increasing of polymeric sample concentration from 25 to 250 µg/mL. Additionally, all membranes unveiled better biocompatibility and respectable biodegradability, suggesting their possible application for advanced wound dressing. Full article
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12 pages, 2731 KiB  
Article
Hemostatic Patches Based on Crosslinked Chitosan Films Applied in Interventional Procedures
by Moon Hyun Lee, Dae Ryeong Lee, Joon Woo Chon and Dong June Chung
Polymers 2021, 13(15), 2402; https://doi.org/10.3390/polym13152402 - 22 Jul 2021
Cited by 6 | Viewed by 2035
Abstract
In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and brittleness. Thus, it was crosslinked [...] Read more.
In this study, we manufactured biocompatible hemostatic crosslinked chitosan (CS) patches and analyzed their physicochemical and biological properties for femoral arterial puncture applications. CS is a representative hemostatic material but has some drawbacks, such as swelling, shrinkage, and brittleness. Thus, it was crosslinked via a 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS) coupling reaction and a nucleophilic addition reaction with citric acid (CA), glutaraldehyde (GTA), and genipin (GP) to remedy its shortcomings. The CSCA (crosslinked CS with CA/EDC), CSGTA (crosslinked CS with GTA), and CSG (crosslinked CS with GP) films showed low swelling degrees and good mechanical properties (excluding CSCA) compared with those of neat CS films. Additionally, every crosslinked CS film coated with thrombin (TB-CS) showed enhanced hemostatic ability in the whole blood clotting and activated partial thromboplastin time tests. Furthermore, the CSCA, CSGTA, and CSGP were nontoxic in an in vitro cell cytotoxicity test (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay) using L-929 mouse fibroblasts cells. Full article
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14 pages, 2944 KiB  
Article
Biosynthesis of Bacterial Cellulose by Extended Cultivation with Multiple Removal of BC Pellicles
by Ekaterina A. Skiba, Nadezhda A. Shavyrkina, Vera V. Budaeva, Anastasia E. Sitnikova, Anna A. Korchagina, Nikolay V. Bychin, Evgenia K. Gladysheva, Igor N. Pavlov, Andrey N. Zharikov, Vladimir G. Lubyansky, Elena N. Semyonova and Gennady V. Sakovich
Polymers 2021, 13(13), 2118; https://doi.org/10.3390/polym13132118 - 28 Jun 2021
Cited by 2 | Viewed by 1900
Abstract
Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. [...] Read more.
Extended cultivation with multiple removal of BC pellicles is proposed herein as a new biosynthetic process for bacterial cellulose (BC). This method enhances the BC surface area by 5–11 times per unit volume of the growth medium, improving the economic efficiency of biosynthesis. The resultant BC gel-films were thin, transparent, and congruent. The degree of polymerization (DP) and elastic modulus (EM) depended on the number of BC pellicle removals, vessel shape, and volume. The quality of BC from removals II–III to VII was better than from removal I. The process scale-up of 1:40 by volume increased DP by 1.5 times and EM by 5 times. A fact was established that the symbiotic Medusomyces gisevii Sa-12 was adaptable to exhausted growth medium: the medium was able to biosynthesize BC for 60 days, while glucose ran low at 24 days. On extended cultivation, DP and EM were found to decline by 39–64% and 57–65%, respectively. The BC gel-films obtained upon removals I–VI were successfully trialed in experimental tension-free hernioplasty. Full article
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13 pages, 3296 KiB  
Article
Controlled Polyelectrolyte Association of Chitosan and Carboxylated Nano-Fibrillated Cellulose by Desalting
by Sarah Amine, Alexandra Montembault, Matthieu Fumagalli, Anayancy Osorio-Madrazo and Laurent David
Polymers 2021, 13(12), 2023; https://doi.org/10.3390/polym13122023 - 21 Jun 2021
Cited by 12 | Viewed by 2477
Abstract
We prepared chitosan (CHI) hydrogels reinforced with highly charged cellulose nanofibrils (CNF) by the desalting method. To this end, the screening of electrostatic interactions between CHI polycation and CNF polyanion was performed by adding NaCl at 0.4 mol/L to the chitosan acetate solution [...] Read more.
We prepared chitosan (CHI) hydrogels reinforced with highly charged cellulose nanofibrils (CNF) by the desalting method. To this end, the screening of electrostatic interactions between CHI polycation and CNF polyanion was performed by adding NaCl at 0.4 mol/L to the chitosan acetate solution and to the cellulose nanofibrils suspension. The polyelectrolyte complexation between CHI polycation and CNF polyanion was then triggered by desalting the CHI/CNF aqueous mixture by multistep dialysis, in large excess of chitosan. Further gelation of non-complexed CHI was performed by alkaline neutralization of the polymer, yielding high reinforcement effects as probed by the viscoelastic properties of the final hydrogel. The results showed that polyelectrolyte association by desalting can be achieved with a polyanionic nanoparticle partner. Beyond obtaining hydrogel with improved mechanical performance, these composite hydrogels may serve as precursor for dried solid forms with high mechanical properties. Full article
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13 pages, 2009 KiB  
Article
The Potential Cytotoxic Activity Enhancement of α-Mangostin in Chitosan-Kappa Carrageenan-Loaded Nanoparticle against MCF-7 Cell Line
by Nasrul Wathoni, Lisna Meylina, Agus Rusdin, Ahmed Fouad Abdelwahab Mohammed, Dorandani Tirtamie, Yedi Herdiana, Keiichi Motoyama, Camelia Panatarani, I Made Joni, Ronny Lesmana and Muchtaridi Muchtaridi
Polymers 2021, 13(11), 1681; https://doi.org/10.3390/polym13111681 - 21 May 2021
Cited by 17 | Viewed by 3087
Abstract
α-mangostin (αM), a xanthone derivative compound isolated from the extract of mangosteen pericarp (Garcinia mangostana L), has potential anticancer properties for breast cancer. However, it has poor solubility in water and low selectivity towards cancer cells. The polymeric nanoparticle formulation approach can [...] Read more.
α-mangostin (αM), a xanthone derivative compound isolated from the extract of mangosteen pericarp (Garcinia mangostana L), has potential anticancer properties for breast cancer. However, it has poor solubility in water and low selectivity towards cancer cells. The polymeric nanoparticle formulation approach can be used to overcome these problems. In this study, a chitosan biopolymer-based αM polymeric nanoparticle formulation was encapsulated using kappa carrageenan (αM-Ch/Cr) as a novel carrier for breast cancer therapy and evaluated for their physicochemical properties, drug release profile, and in vitro cytotoxicity against breast cancer cells (MCF-7). Polymeric nanoparticles formulated with varying concentrations of kappa carrageenan were successfully prepared by ionic gelation and spray pyrolysis techniques. αM-Ch/Cr nanoparticles formed perfectly round particles with a size of 200–400 nm and entrapment efficiency ≥ 98%. In vitro release studies confirmed that αM-Ch/Cr nanoparticles had a sustained release system profile. Interestingly, the formulation of polymeric nanoparticles significantly (p < 0.05) increased the cytotoxicity of αM against MCF-7 cell with IC50 value of 4.7 μg/mL compared to the non-nanoparticle with IC50 of 8.2 μg/mL. These results indicate that αM-Ch/Cr nanoparticles have the potential to improve the physicochemical properties and cytotoxicity effects of αM compounds as breast cancer therapy agents. Full article
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25 pages, 8068 KiB  
Article
Development of Bioinspired Functional Chitosan/Cellulose Nanofiber 3D Hydrogel Constructs by 3D Printing for Application in the Engineering of Mechanically Demanding Tissues
by Arnaud Kamdem Tamo, Ingo Doench, Lukas Walter, Alexandra Montembault, Guillaume Sudre, Laurent David, Aliuska Morales-Helguera, Mischa Selig, Bernd Rolauffs, Anke Bernstein, Daniel Hoenders, Andreas Walther and Anayancy Osorio-Madrazo
Polymers 2021, 13(10), 1663; https://doi.org/10.3390/polym13101663 - 20 May 2021
Cited by 35 | Viewed by 6042
Abstract
Soft tissues are commonly fiber-reinforced hydrogel composite structures, distinguishable from hard tissues by their low mineral and high water content. In this work, we proposed the development of 3D printed hydrogel constructs of the biopolymers chitosan (CHI) and cellulose nanofibers (CNFs), both without [...] Read more.
Soft tissues are commonly fiber-reinforced hydrogel composite structures, distinguishable from hard tissues by their low mineral and high water content. In this work, we proposed the development of 3D printed hydrogel constructs of the biopolymers chitosan (CHI) and cellulose nanofibers (CNFs), both without any chemical modification, which processing did not incorporate any chemical crosslinking. The unique mechanical properties of native cellulose nanofibers offer new strategies for the design of environmentally friendly high mechanical performance composites. In the here proposed 3D printed bioinspired CNF-filled CHI hydrogel biomaterials, the chitosan serves as a biocompatible matrix promoting cell growth with balanced hydrophilic properties, while the CNFs provide mechanical reinforcement to the CHI-based hydrogel. By means of extrusion-based printing (EBB), the design and development of 3D functional hydrogel scaffolds was achieved by using low concentrations of chitosan (2.0–3.0% (w/v)) and cellulose nanofibers (0.2–0.4% (w/v)). CHI/CNF printed hydrogels with good mechanical performance (Young’s modulus 3.0 MPa, stress at break 1.5 MPa, and strain at break 75%), anisotropic microstructure and suitable biological response, were achieved. The CHI/CNF composition and processing parameters were optimized in terms of 3D printability, resolution, and quality of the constructs (microstructure and mechanical properties), resulting in good cell viability. This work allows expanding the library of the so far used biopolymer compositions for 3D printing of mechanically performant hydrogel constructs, purely based in the natural polymers chitosan and cellulose, offering new perspectives in the engineering of mechanically demanding hydrogel tissues like intervertebral disc (IVD), cartilage, meniscus, among others. Full article
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13 pages, 3738 KiB  
Article
A Slot-Die Technique for the Preparation of Continuous, High-Area, Chitosan-Based Thin Films
by Oliver J. Pemble, Maria Bardosova, Ian M. Povey and Martyn E. Pemble
Polymers 2021, 13(10), 1566; https://doi.org/10.3390/polym13101566 - 13 May 2021
Cited by 4 | Viewed by 2365
Abstract
Chitosan-based films have a diverse range of potential applications but are currently limited in terms of commercial use due to a lack of methods specifically designed to produce thin films in high volumes. To address this limitation directly, hydrogels prepared from chitosan, chitosan-tetraethoxy [...] Read more.
Chitosan-based films have a diverse range of potential applications but are currently limited in terms of commercial use due to a lack of methods specifically designed to produce thin films in high volumes. To address this limitation directly, hydrogels prepared from chitosan, chitosan-tetraethoxy silane, also known as tetraethyl orthosilicate (TEOS) and chitosan-glutaraldehyde have been used to prepare continuous thin films using a slot-die technique which is described in detail. By way of preliminary analysis of the resulting films for comparison purposes with films made by other methods, the mechanical strength of the films produced was assessed. It was found that as expected, the hybrid films made with TEOS and glutaraldehyde both show a higher yield strength than the films made with chitosan alone. In all cases, the mechanical properties of the films were found to compare very favorably with similar measurements reported in the literature. In order to assess the possible influence of the direction in which the hydrogel passes through the slot-die on the mechanical properties of the films, testing was performed on plain chitosan samples cut in a direction parallel to the direction of travel and perpendicular to this direction. It was found that there was no evidence of any mechanical anisotropy induced by the slot die process. The examples presented here serve to illustrate how the slot-die approach may be used to create high-volume, high-area chitosan-based films cheaply and rapidly. It is suggested that an approach of the type described here may facilitate the use of chitosan-based films for a wide range of important applications. Full article
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20 pages, 5280 KiB  
Article
Functional Bionanocomposite Fibers of Chitosan Filled with Cellulose Nanofibers Obtained by Gel Spinning
by Sofia Marquez-Bravo, Ingo Doench, Pamela Molina, Flor Estefany Bentley, Arnaud Kamdem Tamo, Renaud Passieux, Francisco Lossada, Laurent David and Anayancy Osorio-Madrazo
Polymers 2021, 13(10), 1563; https://doi.org/10.3390/polym13101563 - 13 May 2021
Cited by 24 | Viewed by 3836
Abstract
Extremely high mechanical performance spun bionanocomposite fibers of chitosan (CHI), and cellulose nanofibers (CNFs) were successfully achieved by gel spinning of CHI aqueous viscous formulations filled with CNFs. The microstructural characterization of the fibers by X-ray diffraction revealed the crystallization of the CHI [...] Read more.
Extremely high mechanical performance spun bionanocomposite fibers of chitosan (CHI), and cellulose nanofibers (CNFs) were successfully achieved by gel spinning of CHI aqueous viscous formulations filled with CNFs. The microstructural characterization of the fibers by X-ray diffraction revealed the crystallization of the CHI polymer chains into anhydrous chitosan allomorph. The spinning process combining acidic–basic–neutralization–stretching–drying steps allowed obtaining CHI/CNF composite fibers of high crystallinity, with enhanced effect at incorporating the CNFs. Chitosan crystallization seems to be promoted by the presence of cellulose nanofibers, serving as nucleation sites for the growing of CHI crystals. Moreover, the preferential orientation of both CNFs and CHI crystals along the spun fiber direction was revealed in the two-dimensional X-ray diffraction patterns. By increasing the CNF amount up to the optimum concentration of 0.4 wt % in the viscous CHI/CNF collodion, Young’s modulus of the spun fibers significantly increased up to 8 GPa. Similarly, the stress at break and the yield stress drastically increased from 115 to 163 MPa, and from 67 to 119 MPa, respectively, by adding only 0.4 wt % of CNFs into a collodion solution containing 4 wt % of chitosan. The toughness of the CHI-based fibers thereby increased from 5 to 9 MJ.m−3. For higher CNFs contents like 0.5 wt %, the high mechanical performance of the CHI/CNF composite fibers was still observed, but with a slight worsening of the mechanical parameters, which may be related to a minor disruption of the CHI matrix hydrogel network constituting the collodion and gel fiber, as precursor state for the dry fiber formation. Finally, the rheological behavior observed for the different CHI/CNF viscous collodions and the obtained structural, thermal and mechanical properties results revealed an optimum matrix/filler compatibility and interface when adding 0.4 wt % of nanofibrillated cellulose (CNF) into 4 wt % CHI formulations, yielding functional bionanocomposite fibers of outstanding mechanical properties. Full article
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16 pages, 3676 KiB  
Article
A Composite Nanosystem as a Potential Tool for the Local Treatment of Glioblastoma: Chitosan-Coated Solid Lipid Nanoparticles Embedded in Electrospun Nanofibers
by Barbara Vigani, Caterina Valentino, Giuseppina Sandri, Roberta Listro, Francesca Fagiani, Simona Collina, Cristina Lanni, Maria Cristina Bonferoni, Carla M. Caramella, Silvia Rossi and Franca Ferrari
Polymers 2021, 13(9), 1371; https://doi.org/10.3390/polym13091371 - 22 Apr 2021
Cited by 13 | Viewed by 2582
Abstract
Glioblastoma multiforme (GBM) is one of the most prevalent and aggressive brain tumors for which there is currently no cure. A novel composite nanosystem (CN), consisting of chitosan-coated Solid Lipid Nanoparticles (c-SLN) embedded in O-carboxymethyl chitosan (O-CMCS)-containing nanofibers (NFs), was proposed as a [...] Read more.
Glioblastoma multiforme (GBM) is one of the most prevalent and aggressive brain tumors for which there is currently no cure. A novel composite nanosystem (CN), consisting of chitosan-coated Solid Lipid Nanoparticles (c-SLN) embedded in O-carboxymethyl chitosan (O-CMCS)-containing nanofibers (NFs), was proposed as a potential tool for the local delivery of lipophilic anti-proliferative drugs. Coacervation was selected as a solvent-free method for the preparation of stearic acid (SA) and behenic acid (BA)-based SLN (SA-SLN and BA-SLN respectively). BA-SLN, containing 0.75% w/w BA sodium salt and 3% w/w poly(vinyl alcohol) (PVA), were selected for the prosecution of the work since they are characterized by the lowest size functional to their subsequent coating and incorporation in nanofibers. BA-SLN were coated with chitosan (CS) by means of a two-step coating method based on the physical absorption of positively charged CS chains on the SLN negative surface. Nile Red (NR), chosen as the hydrophobic model dye, was dissolved in a micellar solution of BA sodium salt and then added with a coacervating solution until pH ≅ 2.5 was reached. Immunocytochemistry analyses highlighted that CS-coated BA-SLN (c-BA-SLN) exhibited a higher accumulation in human glioblastoma cells (U-373) after 6 h than CS-free BA-SLN. Finally, the c-BA-SLN dispersion was blended with a solution consisting of freely soluble polymers (O-CMCS, poly(ethylene oxide) and poloxamer) and then electrospun to obtain NFs with a mean diameter equal to 850 nm. After the NFs dissolution in an aqueous media, c-BA-SLN maintained their physicochemical properties and zeta potential. Full article
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23 pages, 32276 KiB  
Article
Fungal Chitosan-Derived Biomaterials Modified with Kalanchoe pinnata as Potential Hemostatic Agents—Development and Characterization
by Julia Radwan-Pragłowska, Łukasz Janus, Marek Piątkowski, Aleksandra Sierakowska, Tomasz Galek, Ernest Szajna, Dariusz Bogdał and Mirosław Tupaj
Polymers 2021, 13(8), 1300; https://doi.org/10.3390/polym13081300 - 15 Apr 2021
Cited by 6 | Viewed by 2301
Abstract
Massive blood loss is still a great challenge for modern medicine. To stop the hemorrhage during the surgery or after injury apart from suturing or electrocoagulation, the most efficient method of hemostasis restoration is the use of hemostatic agents. Although there are numerous [...] Read more.
Massive blood loss is still a great challenge for modern medicine. To stop the hemorrhage during the surgery or after injury apart from suturing or electrocoagulation, the most efficient method of hemostasis restoration is the use of hemostatic agents. Although there are numerous products on the market, there is still a need for biomaterials that are capable of fast and efficient bleeding management without affecting wound closure or embolism. Chitosan is known for its hemostatic activity; however, its quite poor mechanical properties and heterogenous chemical composition still needs some improvements to become superior compared to biological adhesives. The following study deals with the preparation and evaluation of chitosan-derived natural biomaterials containing Kalanchoe pinnata extract with the potential application as a blood-clotting agent. The materials were obtained under microwave-assisted conditions in two different forms (granules/dressing), whose chemical structure and morphology were studied. Their antioxidant properties have been proven. The chitosan-derived hemostatic agents exhibited superior blood sorption abilities and lack of cytotoxicity to L929 mouse fibroblasts. The study also showed the differences in biological properties depending on their preparation method. The potential mechanism of action was proposed as well as their potential in hemostasis revival. Full article
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22 pages, 3779 KiB  
Article
Microencapsulation of Erlotinib and Nanomagnetite Supported in Chitosan as Potential Oncologic Carrier
by Galo Cárdenas-Triviño, Sebastián Monsalve-Rozas and Luis Vergara-González
Polymers 2021, 13(8), 1244; https://doi.org/10.3390/polym13081244 - 12 Apr 2021
Viewed by 1671
Abstract
Microcapsules (MC) based on chitosan (CH) and including nano-magnetite and erlotinib were synthesized. The microparticles were characterized by SEM, FT-IR and TGA. The percentage of encapsulation was determined, as well as its microbiological activity. Finally, the effectiveness of the formulation was evaluated in [...] Read more.
Microcapsules (MC) based on chitosan (CH) and including nano-magnetite and erlotinib were synthesized. The microparticles were characterized by SEM, FT-IR and TGA. The percentage of encapsulation was determined, as well as its microbiological activity. Finally, the effectiveness of the formulation was evaluated in terms of cell viability and/or toxicity when compared with the reference drug. The formulation used to prepare the microcapsules showed some bacteriostatic properties. The characterization of microcapsules exhibited amorphous spherical shape and average size of 1.29, 1.58 and 1.62 mm for chitosan, chitosan + nanomagnetite and chitosan + nanomagnetite + erlotinib, respectively. The infrared spectra showed characteristic bands of the erlotinib and magnetite, confirming its internalization. The thermogravimetric analyzes indicated that the materials do not undergo changes at optimum working temperatures. The HPLC analysis showed a 52% of encapsulation. Finally, the formulation probed had lower effectiveness and less cytotoxicity, than the drug without encapsulating “in vitro” studies. For that reason several assays are in progress. Full article
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10 pages, 6681 KiB  
Article
Magnetic Resonance Imaging of Transplanted Porcine Neonatal Pancreatic Cell Clusters Labeled with Chitosan-Coated Superparamagnetic Iron Oxide Nanoparticles in Mice
by Jyuhn-Huarng Juang, Jiun-Jie Wang, Chia-Rui Shen, Chen-Yi Chen, Chen-Wei Kao, Chen-Ling Chen, Sung-Han Lin, Shu-Ting Wu, Wan-Chun Li and Zei-Tsan Tsai
Polymers 2021, 13(8), 1238; https://doi.org/10.3390/polym13081238 - 11 Apr 2021
Cited by 6 | Viewed by 2039
Abstract
Neonatal pancreatic cell clusters (NPCCs) are potential tissues for the treatment of diabetes. Different from adult cells, they continuously proliferate and differentiate after transplantation. In this study, we utilized magnetic resonance imaging (MRI) to detect and monitor implanted NPCCs. NPCCs were isolated from [...] Read more.
Neonatal pancreatic cell clusters (NPCCs) are potential tissues for the treatment of diabetes. Different from adult cells, they continuously proliferate and differentiate after transplantation. In this study, we utilized magnetic resonance imaging (MRI) to detect and monitor implanted NPCCs. NPCCs were isolated from one-day-old neonatal pigs, cultured for three days, and then incubated overnight with the contrast agent chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles. In vitro, Prussian blue staining and MR scans of CSPIO-labeled NPCCs were performed. In vivo, we transplanted 2000 CSPIO-labeled NPCCs under the kidney capsule of nondiabetic nude mice. Recipients were scanned with 7.0T MRI. Grafts were removed for histology with insulin and Prussian blue staining. After being incubated overnight with CSPIO, NPCCs showed positive iron staining and appeared as dark spots on MR scans. After transplantation of CSPIO-labeled NPCCs, persistent hypointense areas were observed at recipients’ implant sites for up to 54 days. Moreover, histology showed colocalization of the insulin and iron staining in 15-, 51- and 55-day NPCC grafts. Our results indicate that transplanted NPCCs survived and differentiated to β cells after transplantation, and that MRI is a useful tool for the detection and monitoring of CSPIO-labeled NPCC grafts. Full article
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26 pages, 8572 KiB  
Article
PHB/CHIT Scaffold as a Promising Biopolymer in the Treatment of Osteochondral Defects—An Experimental Animal Study
by Eva Petrovova, Marek Tomco, Katarina Holovska, Jan Danko, Lenka Kresakova, Katarina Vdoviakova, Veronika Simaiova, Filip Kolvek, Petra Hornakova, Teodor Toth, Jozef Zivcak, Peter Gal, David Sedmera, Lenka Luptakova and Lubomir Medvecky
Polymers 2021, 13(8), 1232; https://doi.org/10.3390/polym13081232 - 11 Apr 2021
Cited by 10 | Viewed by 3002
Abstract
Biopolymer composites allow the creation of an optimal environment for the regeneration of chondral and osteochondral defects of articular cartilage, where natural regeneration potential is limited. In this experimental study, we used the sheep animal model for the creation of knee cartilage defects. [...] Read more.
Biopolymer composites allow the creation of an optimal environment for the regeneration of chondral and osteochondral defects of articular cartilage, where natural regeneration potential is limited. In this experimental study, we used the sheep animal model for the creation of knee cartilage defects. In the medial part of the trochlea and on the medial condyle of the femur, we created artificial defects (6 × 3 mm2) with microfractures. In four experimental sheep, both defects were subsequently filled with the porous acellular polyhydroxybutyrate/chitosan (PHB/CHIT)-based implant. Two sheep had untreated defects. We evaluated the quality of the newly formed tissue in the femoral trochlea defect site using imaging (X-ray, Computer Tomography (CT), Magnetic Resonance Imaging (MRI)), macroscopic, and histological methods. Macroscopically, the surface of the treated regenerate corresponded to the niveau of the surrounding cartilage. X-ray examination 6 months after the implantation confirmed the restoration of the contour in the subchondral calcified layer and the advanced rate of bone tissue integration. The CT scan revealed a low regenerative potential in the bone zone of the defect compared to the cartilage zone. The percentage change in cartilage density at the defect site was not significantly different to the reference area (0.06–6.4%). MRI examination revealed that the healing osteochondral defect was comparable to the intact cartilage signal on the surface of the defect. Hyaline-like cartilage was observed in most of the treated animals, except for one, where the defect was repaired with fibrocartilage. Thus, the acellular, chitosan-based biomaterial is a promising biopolymer composite for the treatment of chondral and osteochondral defects of traumatic character. It has potential for further clinical testing in the orthopedic field, primarily with the combination of supporting factors. Full article
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11 pages, 2860 KiB  
Article
Nanofibrillated Cellulose-Based Aerogels Functionalized with Tajuva (Maclura tinctoria) Heartwood Extract
by Rodrigo Coldebella, Marina Gentil, Camila Berger, Henrique W. Dalla Costa, Cristiane Pedrazzi, Jalel Labidi, Rafael A. Delucis and André L. Missio
Polymers 2021, 13(6), 908; https://doi.org/10.3390/polym13060908 - 16 Mar 2021
Cited by 6 | Viewed by 2367
Abstract
Aerogels are 3-D nanostructures of non-fluid colloidal interconnected porous networks consisting of loosely packed bonded particles that are expanded throughout their volume by gas and exhibit ultra-low density and high specific surface area. Cellulose-based aerogels can be obtained from hydrogels through a drying [...] Read more.
Aerogels are 3-D nanostructures of non-fluid colloidal interconnected porous networks consisting of loosely packed bonded particles that are expanded throughout their volume by gas and exhibit ultra-low density and high specific surface area. Cellulose-based aerogels can be obtained from hydrogels through a drying process, replacing the solvent (water) with air and keeping the pristine three-dimensional arrangement. In this work, hybrid cellulose-based aerogels were produced and their potential for use as dressings was assessed. Nanofibrilated cellulose (NFC) hydrogels were produced by a co-grinding process in a stone micronizer using a kraft cellulosic pulp and a phenolic extract from Maclura tinctoria (Tajuva) heartwood. NFC-based aerogels were produced by freeze followed by lyophilization, in a way that the Tajuva extract acted as a functionalizing agent. The obtained aerogels showed high porosity (ranging from 97% to 99%) and low density (ranging from 0.025 to 0.040 g·cm−3), as well a typical network and sheet-like structure with 100 to 300 μm pores, which yielded compressive strengths ranging from 60 to 340 kPa. The reached antibacterial and antioxidant activities, percentage of inhibitions and water uptakes suggest that the aerogels can be used as fluid absorbers. Additionally, the immobilization of the Tajuva extract indicates the potential for dentistry applications. Full article
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21 pages, 1550 KiB  
Review
Antimicrobial Actions and Applications of Chitosan
by Cai-Ling Ke, Fu-Sheng Deng, Chih-Yu Chuang and Ching-Hsuan Lin
Polymers 2021, 13(6), 904; https://doi.org/10.3390/polym13060904 - 15 Mar 2021
Cited by 259 | Viewed by 14422
Abstract
Chitosan is a naturally originating product that can be applied in many areas due to its biocompatibility, biodegradability, and nontoxic properties. The broad-spectrum antimicrobial activity of chitosan offers great commercial potential for this product. Nevertheless, the antimicrobial activity of chitosan varies, because this [...] Read more.
Chitosan is a naturally originating product that can be applied in many areas due to its biocompatibility, biodegradability, and nontoxic properties. The broad-spectrum antimicrobial activity of chitosan offers great commercial potential for this product. Nevertheless, the antimicrobial activity of chitosan varies, because this activity is associated with its physicochemical characteristics and depends on the type of microorganism. In this review article, the fundamental properties, modes of antimicrobial action, and antimicrobial effects-related factors of chitosan are discussed. We further summarize how microorganisms genetically respond to chitosan. Finally, applications of chitosan-based biomaterials, such as nanoparticles and films, in combination with current clinical antibiotics or antifungal drugs, are also addressed. Full article
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14 pages, 1062 KiB  
Article
Effect of Chitosan Coating Incorporated with Artemisia fragrans Essential Oil on Fresh Chicken Meat during Refrigerated Storage
by Milad Yaghoubi, Ali Ayaseh, Kazem Alirezalu, Zabihollah Nemati, Mirian Pateiro and José M. Lorenzo
Polymers 2021, 13(5), 716; https://doi.org/10.3390/polym13050716 - 26 Feb 2021
Cited by 40 | Viewed by 3235
Abstract
The present study was conducted to assess the impact of chitosan coating (1%) containing Artemisia fragrans essential oil (500, 1000, and 1500 ppm) as antioxidant and antimicrobial agent on the quality properties and shelf life of chicken fillets during refrigerated storage. After packaging [...] Read more.
The present study was conducted to assess the impact of chitosan coating (1%) containing Artemisia fragrans essential oil (500, 1000, and 1500 ppm) as antioxidant and antimicrobial agent on the quality properties and shelf life of chicken fillets during refrigerated storage. After packaging meat samples, physicochemical, microbiological, and organoleptic attributes were evaluated at 0, 3, 6, 9, and 12 days at 4 °C. The results revealed that applied chitosan (CH) coating in combination with Artemisia fragrans essential oils (AFEOs) had no significant (p < 0.05) effects on proximate composition among treatments. The results showed that the incorporation of AFEOs into CH coating significantly reduced (p < 0.05) pH, thiobarbituric acid reactive substances (TBARS), and total volatile base nitrogen (TVB-N), especially for 1% CH coating + 1500 ppm AFEOs, with values at the end of storage of 5.58, 1.61, and 2.53, respectively. The coated samples also displayed higher phenolic compounds than those obtained by uncoated samples. Coated chicken meat had, significantly (p < 0.05), the highest inhibitory effects against microbial growth. The counts of TVC (total viable counts), coliforms, molds, and yeasts were significantly lower (p < 0.05) in 1% CH coating + 1500 ppm AFEOs fillets (5.32, 3.87, and 4.27 Log CFU/g, respectively) at day 12. Organoleptic attributes of coated samples also showed the highest overall acceptability scores than uncoated ones. Therefore, the incorporation of AFEOs into CH coating could be effectively used for improving stability and shelf life of chicken fillets during refrigerated storage. Full article
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16 pages, 1483 KiB  
Article
Chitosan Nanocarrier Entrapping Hydrophilic Drugs as Advanced Polymeric System for Dual Pharmaceutical and Cosmeceutical Application: A Comprehensive Analysis Using Box-Behnken Design
by Sara A. Abosabaa, Aliaa N. ElMeshad and Mona G. Arafa
Polymers 2021, 13(5), 677; https://doi.org/10.3390/polym13050677 - 24 Feb 2021
Cited by 23 | Viewed by 2687
Abstract
The objective of the present research is to propose chitosan as a nanocarrier for caffeine—a commonly used drug in combating cellulite. Being a hydrophilic drug, caffeine suffers from insufficient topical penetration upon application on the skin. Chitosan nanoparticles loaded with caffeine were prepared [...] Read more.
The objective of the present research is to propose chitosan as a nanocarrier for caffeine—a commonly used drug in combating cellulite. Being a hydrophilic drug, caffeine suffers from insufficient topical penetration upon application on the skin. Chitosan nanoparticles loaded with caffeine were prepared via the ionic gelation technique and optimized according to a Box–Behnken design. The effect of (A) chitosan concentration, (B) chitosan solution pH, and (C) chitosan to sodium tripolyphosphate mass ratio on (Y1) entrapment efficiency percent, (Y2) particle size, (Y3) polydispersity index, and (Y4) zeta potential were studied. Subsequently, the desired constraints on responses were applied, and validation of the optimization procedure was confirmed by the parameters exhibited by the optimal formulation. A caffeine entrapment efficiency percent of 17.25 ± 1.48%, a particle size of 173.03 ± 4.32 nm, a polydispersity index of 0.278 ± 0.01, and a surface charge of 41.7 ± 3.0 mV were attained. Microscopical evaluation using transmission electron microscope revealed a typical spherical nature of the nanoparticles arranged in a network with a further confirmation of the formation of particles in the nano range. The results proved the successful implementation of the Box–Behnken design for optimization of chitosan-based nanoparticles in the field of advanced polymeric systems for pharmaceutical and cosmeceutical applications. Full article
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15 pages, 3635 KiB  
Article
Gelatin/Chitosan Bilayer Patches Loaded with Cortex Phellodendron amurense/Centella asiatica Extracts for Anti-Acne Application
by Chi-Wen Kuo, Yi-Fang Chiu, Min-Hua Wu, Ming-Hsien Li, Cheng-Nan Wu, Wan-Sin Chen and Chiung-Hua Huang
Polymers 2021, 13(4), 579; https://doi.org/10.3390/polym13040579 - 15 Feb 2021
Cited by 12 | Viewed by 9333
Abstract
Acne is a chronic inflammatory skin disease that often occurs with anaerobic Propionibacterium acnes (P. acnes). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic [...] Read more.
Acne is a chronic inflammatory skin disease that often occurs with anaerobic Propionibacterium acnes (P. acnes). Anti-acne patches, made of hydrocolloid or hydrogel, have become a popular way of topical treatment. The outer water-impermeable layer of commercial patches might create hypoxic conditions and promote P. acnes growth. In this study, gelatin/chitosan (GC) bilayer patches were prepared at different temperatures that included room temperature (RT), −20 °C/RT, and −80 °C/RT. The most promising GC bilayer patch (−80 °C /RT) contained a dense upper layer for protection from bacteria and infection and a porous lower layer for absorbing pus and fluids from pimples. The anti-acne bilayer patch was loaded with Cortex Phellodendri amurensis (PA) and Centella asiatica (CA) extracts. PA extract could inhibit the growth of P. acnes and CA extract was reported to improve wound healing and reduce scar formation. Moreover, the water retention rate, weight loss rate, antibacterial activity, and in vitro cytotoxicity of the patches were investigated. The porous structure of the patches promoted water retention and contributed to absorbing the exudate when used on open acne wounds. The GC bilayer patches loaded with PA/CA extracts were demonstrated to inhibit the growth of P. acnes, and accelerate the skin fibroblast cell viability. Based on their activities and characteristics, the GC bilayer patches with PA/CA extract prepared at −80 °C/RT obtain the potential for the application of acne spot treatment. Full article
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13 pages, 4883 KiB  
Article
Glycerol Assisted Pretreatment of Lignocellulose Wheat Straw Materials as a Promising Approach for Fabrication of Sustainable Fibrous Filler for Biocomposites
by Hamayoun Mahmood, Saqib Mehmood, Ahmad Shakeel, Tanveer Iqbal, Mohsin Ali Kazmi, Abdul Rehman Khurram and Muhammad Moniruzzaman
Polymers 2021, 13(3), 388; https://doi.org/10.3390/polym13030388 - 26 Jan 2021
Cited by 6 | Viewed by 2630
Abstract
Glycerol pretreatment is a promising method for the environmentally-friendly transformation of lignocellulosic materials into sustainable cellulose-rich raw materials (i.e., biopolymer) to fabricate biocomposites. Here, a comparison of aqueous acidified glycerol (AAG) pretreatment of wheat straw (WS) with alkaline, hot water, and dilute acid [...] Read more.
Glycerol pretreatment is a promising method for the environmentally-friendly transformation of lignocellulosic materials into sustainable cellulose-rich raw materials (i.e., biopolymer) to fabricate biocomposites. Here, a comparison of aqueous acidified glycerol (AAG) pretreatment of wheat straw (WS) with alkaline, hot water, and dilute acid pretreatments on the thermal and mechanical characteristics of their fabricated composite board is presented. A comparison of total energy expenditure during WS pretreatment with AAG and other solutions was estimated and a comparative influence of AAG processing on lignocellulosic constituents and thermal stability of WS fiber was studied. Results imply that AAG pretreatment was superior in generating cellulose-rich fiber (CRF) as compared to other pretreatments and enhanced the cellulose contents by 90% compared to raw WS fiber. Flexural strength of acidic (40.50 MPa) and hot water treated WS composite (38.71 MPa) was higher compared to the value of 33.57 MPa for untreated composite, but AAG-treated composites exhibited lower values of flexural strength (22.22 MPa) compared to untreated composite samples. Conversely, AAG pretreatment consumed about 56% lesser energy for each kg of WS processed as compared to other pretreatments. These findings recognize that glycerol pretreatment could be a clean and new pretreatment strategy to convert agricultural waste into high-quality CRF as a sustainable raw material source for engineered biocomposite panels. Full article
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2020

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16 pages, 4398 KiB  
Article
Hydrogels Based on Imino-Chitosan Amphiphiles as a Matrix for Drug Delivery Systems
by Daniela Ailincai, William Porzio and Luminita Marin
Polymers 2020, 12(11), 2687; https://doi.org/10.3390/polym12112687 - 14 Nov 2020
Cited by 20 | Viewed by 2406
Abstract
This paper reports new formulations based on chitosan, citral, and diclofenac sodium salt (DCF). The central idea was to encapsulate an anionic drug into a polycationic hydrogel matrix in order to increase the intermolecular forces between them and thus to ensure slower drug [...] Read more.
This paper reports new formulations based on chitosan, citral, and diclofenac sodium salt (DCF). The central idea was to encapsulate an anionic drug into a polycationic hydrogel matrix in order to increase the intermolecular forces between them and thus to ensure slower drug release, while citral was used as a penetration enhancer to assure efficient delivery of the drug. Hydrogels without drug were also synthesized and used as a reference. The structure, morphology, and supramolecular architecture of the drug delivery systems were evaluated by FTIR spectroscopy, scanning electron microscopy, polarized optical microscopy, and wide-angle X-ray diffraction. The drug release kinetics was monitored in vitro by UV-VIS spectroscopy, in physiological conditions, while the enzymatic and hydrolytic degradability of the hydrogels were evaluated in the presence of lysozyme and phosphate buffer saline (PBS), at 37 °C. All of the data revealed that the anionic DCF was strongly anchored into the polycationic matrix and the drug was slowly released over 7 days. Moreover, the release rate can be controlled by simple variation of the molar ratio between the polycationic chitosan and lipophilic citral. Full article
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16 pages, 3883 KiB  
Review
A Mini-Review on Chitosan-Based Hydrogels with Potential for Sustainable Agricultural Applications
by Regina Michalik and Ilona Wandzik
Polymers 2020, 12(10), 2425; https://doi.org/10.3390/polym12102425 - 21 Oct 2020
Cited by 71 | Viewed by 7763
Abstract
Agriculture is an important sector of the economy, but this industry consumes significant amounts of water, which is a precious and limited natural resource. Irrigation techniques and efforts to mitigate water usage influence the growth, survival, and yield of crops. However, superabsorbent polymers [...] Read more.
Agriculture is an important sector of the economy, but this industry consumes significant amounts of water, which is a precious and limited natural resource. Irrigation techniques and efforts to mitigate water usage influence the growth, survival, and yield of crops. However, superabsorbent polymers in combination with fertilizers can be employed to obtain sustained release of nutrients and improved water retention capacity of the soil. Despite significant recent progress in this area involving synthetic polyacrylate hydrogels, there are no industrially applicable solutions exhibiting similar performance using natural biopolymers or synthetic polymers enriched with natural components. This review focuses on biodegradable chitosan-based hydrogels (both natural and semi-synthetic), and discusses their potential agricultural and horticultural applications. The methods for synthesizing hydrogels via physical or chemical crosslinking, and the resulting functional properties of recently reported hydrogels, such as water retention and release of active ingredients, are presented herein. Full article
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16 pages, 3328 KiB  
Article
Hemostatic Enhancement via Chitosan Is Independent of Classical Clotting Pathways—A Quantitative Study
by Kuan-Yu Chen, Yen-Cheng Chen, Tzu-Hsin Lin, Cheng-Ying Yang, Ya-Wen Kuo and U. Lei
Polymers 2020, 12(10), 2391; https://doi.org/10.3390/polym12102391 - 17 Oct 2020
Cited by 8 | Viewed by 2121
Abstract
Hemostasis is a process causing bleeding to stop, and it is known from the literature that hemostasis can be enhanced using chitosan on wound gauzes. We proposed here a continuous flow-through device, with the test blood flowing through the gauze sample at a [...] Read more.
Hemostasis is a process causing bleeding to stop, and it is known from the literature that hemostasis can be enhanced using chitosan on wound gauzes. We proposed here a continuous flow-through device, with the test blood flowing through the gauze sample at a constant flow rate and the pressure drop across the gauze measured, for assessing the hemostatic performance of the gauze. Experiments were performed using the device with both whole blood and washed blood (with clotting factors and platelets removed from the whole blood), and their results agree with each other within 10% discrepancy, indicating quantitatively that hemostatic enhancement via chitosan is essentially independent of classical clotting pathways, which was demonstrated qualitatively through animal tests in the literature. The proposed device and method can be applied for evaluating quantitatively the hemostatic performance of various gauzes in a flowing blood environment (in comparison with static tests) with less test blood (20–60% less, in comparison with that of a flow-through device driven by a constant pressure gradient), and are thus, helpful for designing better wound gauzes. In particular, it is effective to enhance the hemostatic performance further (additional 30%) through acidification (changing the amino group to the ammonium group) of the gauze for chitosan-based wound gauzes. Full article
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18 pages, 4974 KiB  
Article
Removal of Cu(II) by Fixed-Bed Columns Using Alg-Ch and Alg-ChS Hydrogel Beads: Effect of Operating Conditions on the Mass Transfer Zone
by Ilse Paulina Verduzco-Navarro, Nely Rios-Donato, Carlos Federico Jasso-Gastinel, Álvaro de Jesús Martínez-Gómez and Eduardo Mendizábal
Polymers 2020, 12(10), 2345; https://doi.org/10.3390/polym12102345 - 13 Oct 2020
Cited by 19 | Viewed by 2097
Abstract
The removal of Cu(II) ions from aqueous solutions at a pH of 5.0 was carried out using fixed-bed columns packed with alginate-chitosan (Alg-Ch) or alginate-chitosan sulfate (Alg-ChS) hydrogel beads. The effect of the initial Cu(II) concentration, flow rate, pH, and height of the [...] Read more.
The removal of Cu(II) ions from aqueous solutions at a pH of 5.0 was carried out using fixed-bed columns packed with alginate-chitosan (Alg-Ch) or alginate-chitosan sulfate (Alg-ChS) hydrogel beads. The effect of the initial Cu(II) concentration, flow rate, pH, and height of the column on the amount of Cu removed by the column at the breakpoint and at the exhaustion point is reported. The pH of the solution at the column’s exit was initially higher than that at the entrance, and then decreased slowly. This pH increase was attributed to proton transfer from the aqueous solution to the amino and COO groups of the hydrogel. The effect of operating conditions on the mass transfer zone (MTZ) and the length of the unused bed (HLUB) is reported. At the lower flow rate and lower Cu(II) concentration used, the MTZ was completely developed and the column operated efficiently; by increasing column height, the MTZ has a better opportunity to develop fully. Experimental data were fitted to the fixed-bed Thomas model using a non-linear regression analysis and a good correspondence between experimental and Thomas model curves was observed. Full article
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22 pages, 6211 KiB  
Article
Traditional Sensory Evaluation and Bionic Electronic Nose as Innovative Tools for the Packaging Performance Evaluation of Chitosan Film
by Wei Song, Jian Xu, Lili Ren, Li Guo, Jin Tong, Liyan Wang and Zhiyong Chang
Polymers 2020, 12(10), 2310; https://doi.org/10.3390/polym12102310 - 09 Oct 2020
Cited by 6 | Viewed by 2213
Abstract
Inspired by the natural epidermis of animals and plants with antioxidant and antibacterial properties, the aim of this research was to characterize and analyze the effects of the chitosan concentrations on properties of glycerol plasticized chitosan (GPC) film and to investigate the suitability [...] Read more.
Inspired by the natural epidermis of animals and plants with antioxidant and antibacterial properties, the aim of this research was to characterize and analyze the effects of the chitosan concentrations on properties of glycerol plasticized chitosan (GPC) film and to investigate the suitability of sensory evaluation and bionic electronic nose (b-electronic nose) detection to assess the freshness of ground beef packaged in the GPC film. The increase in chitosan concentration resulted in an increase in solubility value, total color differences and color intensity of chitosan films. The water vapor permeability (WVP) of the GPC films decreased with the increasing of the chitosan concentration and then increased at higher chitosan concentrations. Longer storage time led to poorer freshness of the ground beef and the GPC film could keep beef samples fresher and delay the deterioration of the beef. Both the traditional sensory evaluation and b-electronic nose technology were fit for evaluating the quality and shelf-life of ground beef, which could advantageously be applied in the future for analyzing other bionic food packaging materials. Full article
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