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17 pages, 9029 KiB

Open AccessArticle

The Impact of Growth Years on the Medicinal Material Characteristics and Metabolites of Stellaria dichotoma L. var. lanceolata Bge. Reveals the Optimal Harvest Age

by Zhenkai Li, Lu Feng, Hong Wang, Lin Zhang, Haishan Li, Yanqing Li, Pilian Niu, Gege Tian, Yan Yang, Xiangui Mei and Li Peng

Plants 2023, 12(12), 2286; https://doi.org/10.3390/plants12122286 - 12 Jun 2023

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Abstract

The original plant of Chinese medicine Stellariae Radix (Yin Chai Hu) is Stellaria dichotoma L. var. lanceolata Bge (abbreviated as SDL). SDL is a perennial herbaceous plant and a characteristic crop in Ningxia. Growth years are vital factors that affect the quality of [...] Read more.

The original plant of Chinese medicine Stellariae Radix (Yin Chai Hu) is Stellaria dichotoma L. var. lanceolata Bge (abbreviated as SDL). SDL is a perennial herbaceous plant and a characteristic crop in Ningxia. Growth years are vital factors that affect the quality of perennial medicinal materials. This study aims to investigate the impact of growth years on SDL and screen for the optimal harvest age by comparing the medicinal material characteristics of SDL with different growth years. Additionally, metabolomics analysis using UHPLC-Q-TOF MS was employed to investigate the impact of growth years on the accumulation of metabolites in SDL. The results show that the characteristics of medicinal materials and the drying rate of SDL gradually increase with the increase in growth years. The fastest development period of SDL occurred during the first 3 years, after which the development slowed down. Medicinal materials characteristics of 3-year-old SDL exhibited mature qualities with a high drying rate, methanol extract content, and the highest content of total sterols and total flavonoids. A total of 1586 metabolites were identified, which were classified into 13 major classes with more than 50 sub-classes. Multivariate statistical analysis indicated significant differences in the diversity of metabolites of SDL in different growth years, with greater differences observed in metabolites as the growth years increased. Moreover, different highly expressed metabolites in SDL at different growth years were observed: 1–2 years old was beneficial to the accumulation of more lipids, while 3–5 years old was conducive to accumulating more alkaloids, benzenoids, etc. Furthermore, 12 metabolites accumulating with growth years and 20 metabolites decreasing with growth years were screened, and 17 significantly different metabolites were noted in 3-year-old SDL. In conclusion, growth years not only influenced medicinal material characteristics, drying rate, content of methanol extract, and total sterol and flavonoid contents, but also had a considerable effect on SDL metabolites and metabolic pathways. SDL planted for 3 years presented the optimum harvest time. The screened significantly different metabolites with biological activity, such as rutin, cucurbitacin e, isorhamnetin-3-o-glucoside, etc., can be utilized as potential quality markers of SDL. This research provides references for studying the growth and development of SDL medicinal materials, the accumulation of metabolites, and the selection of optimal harvest time. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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15 pages, 824 KiB

Open AccessArticle

Agronomic Evaluation and Chemical Characterization of Lavandula latifolia Medik. under the Semiarid Conditions of the Spanish Southeast

by Gustavo J. Cáceres-Cevallos, María Quílez, Gonzalo Ortiz de Elguea-Culebras, Enrique Melero-Bravo, Raúl Sánchez-Vioque and María J. Jordán

Plants 2023, 12(10), 1986; https://doi.org/10.3390/plants12101986 - 15 May 2023

Cited by 1 | Viewed by 960

Abstract

Lavandula latifolia is one of the main rainfed crops of aromatic and medicinal plants produced in Spain. As a global concern, the agronomic productivity of this aromatic crop is also threatened by the consequences of imminent climate change. On this basis, the study [...] Read more.

Lavandula latifolia is one of the main rainfed crops of aromatic and medicinal plants produced in Spain. As a global concern, the agronomic productivity of this aromatic crop is also threatened by the consequences of imminent climate change. On this basis, the study of the agronomic production of two drought-tolerant ecotypes, after three years of cultivations practices, constitutes the main objective of the present study. For this trial, clones of the two pre-selected ecotypes, along with clones from two commercial plants (control), were grown in an experimental plot. The main results confirmed an increase in biomass and essential oil production with plant age. The essential oil chemotype defined by 1,8-cineol, linalool, and camphor was maintained over time, but a decrease in 1,8-cineol in the benefit of linalool was detected. In the phenolic profile, 14 components were identified, with salvianic acid and a rosmarinic acid derivate being the main compounds quantified. These phenolic extracts showed potent in vitro antioxidant capacity, and after the second year of cultivation practices, both phenolic compounds and antioxidant capacity remained stable. Thus, under semiarid conditions, L. latifolia drought-tolerant ecotypes reach a good level of production after the second year of crop establishment. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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25 pages, 2521 KiB

Open AccessArticle

The Effect of Irrigation and Humic Acid on the Plant Yield and Quality of Sweet Basil (Ocimum basilicum L.) with Mulching Application under Semi-Arid Ecological Conditions

by Melike Sayarer, Zehra Aytaç and Mine Kürkçüoğlu

Plants 2023, 12(7), 1522; https://doi.org/10.3390/plants12071522 - 31 Mar 2023

Cited by 1 | Viewed by 1301

Abstract

The adoption of suitable irrigation levels (IRL), humic acid doses (HAD) and soil mulching (SM) are important tools for improving the morpho-physiological and biochemical traits of medicinal and aromatic plants. Ocimum basilicum L. cultivated under four IRL: IRL 100 = 100% FC–IRL 75 [...] Read more.

The adoption of suitable irrigation levels (IRL), humic acid doses (HAD) and soil mulching (SM) are important tools for improving the morpho-physiological and biochemical traits of medicinal and aromatic plants. Ocimum basilicum L. cultivated under four IRL: IRL 100 = 100% FC–IRL 75 = 75% FC–IRL 50 = 50% FC–IRL 25 = 25% FC and four HAD: HA 0 = 0.0 Lha⁻¹–HA 10 = 10.0 Lha⁻¹–HA 20 = 20.0 L ha⁻¹–HA 40 = 40.0 L ha⁻¹ were applied in order to evaluate morpho-physiological and biochemical traits under the ecological conditions of Eskişehir in 2016 and 2017. A second trial was conducted with black plastic soil mulch (SM) and compared with the control plots (CP) in 2016. The experiment was arranged in a randomized complete block design with split plots and three replications. The plant height (PH), fresh herb yield (FHY), dry herb yield (DHY), dry leaf yield (DLY), protein ratio (PR), and main essential oil compounds (MEOC) of Ocimum basilicum L. increased and the essential oil ratio (EOR) and essential oil yield (EOY) decreased with increasing IRL (IRL 100 and IRL 75). FHY (7268.3 and 7472.7 kg ha⁻¹) and DLY (635.3 and 637.5 kg ha⁻¹) increased with increasing HAD (HA 20 and HA 40) compared to the values of FHY and DLY at HA 0 (6852.6 and 587.0 respectively). The SM application at IRL 50 increased the PH between 8.8 and 13.5%, FHY 11.7 and 16.7%, DLY 22.5 and 29.2%, and at IRL 75 the EOY between 20.0 and 23.9% compared to CP. In addition, PH, FHY, DLY, and EOY were highest at HA 40 and HA 20. The MEOC (linalool, 1,8-cineole, and (E) – β-bergamotene) under SM were more pronounced at IRL 25 and IRL 50 compared to CP. HA particularly improved FHY, DLY, and the main essential oil compounds that can be considered plant biostimulants, which were defined by several studies and regulations. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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15 pages, 2306 KiB

Open AccessArticle

Improvement in Essential Oil Quantity and Quality of Thyme (Thymus vulgaris L.) by Integrative Application of Chitosan Nanoparticles and Arbuscular Mycorrhizal Fungi under Water Stress Conditions

by Mostafa Amani Machiani, Abdollah Javanmard, Ali Ostadi and Khoshnood Alizadeh

Plants 2023, 12(7), 1422; https://doi.org/10.3390/plants12071422 - 23 Mar 2023

Viewed by 1480

Abstract

Water stress is one of the critical abiotic stresses and limiting factors in the productivity of plants, especially in arid and semi-arid regions. In recent years, the application of bio-fertilizer and stress-modulating nanoparticles (NPs) is known as one of the eco-friendly strategies for [...] Read more.

Water stress is one of the critical abiotic stresses and limiting factors in the productivity of plants, especially in arid and semi-arid regions. In recent years, the application of bio-fertilizer and stress-modulating nanoparticles (NPs) is known as one of the eco-friendly strategies for improving plants quantity and quality under stressful conditions. In order to achieve the desirable essential oil (EO) quality and quantity of thyme in water deficit conditions, a 2-year field experiment was carried out as a split plot based on the randomized complete block design (RCBD), with 12 treatments and three replications. The treatments included different irrigation levels, containing irrigation at 80% field capacity (FC80) as no stress, 60% FC as moderate water stress (FC60) and 40% FC as severe water stress (FC40), as well as four different fertilizer sources, including non-application of fertilizer (control), application of arbuscular mycorrhizal fungi (AMF), chitosan NPs (CHT) and co-application of AMF+CHT NPs. The results demonstrated that the dry yield of thyme decreased by 13% and 40.3% under FC60 and FC40 water stress conditions. However, co-application of AMF+CHT NPs enhanced the dry yield of thyme by 21.7% in comparison to the control (non-application of fertilizer). The maximum EO content (2.03%) and EO yield (10.04 g 7 g m⁻²) of thyme were obtained under moderate water stress (FC60) fertilized with AMF+CHT NPs. Co-application of AMF+CHT NPs enhanced the EO content and EO yield of thyme by 17.1% and 42.7%, respectively. Based on the GC-MS and GC-FID analysis, 38 constituents were identified in the thyme EO, with the major constituents being thymol (35.64–41.31%), p-cymene (16.35–19.38%), γ-terpinene (12.61–13.98%) and carvacrol (2.78–3.93%) respectively. The highest content of thymol and γ-terpinene was obtained under moderate water stress (FC60) fertilized with AMF+CHT NPs. In addition, the highest content of p-cymene and carvacrol was observed in the severe water stress (FC40) fertilized with AMF+CHT NPs. The present research suggests that the co-application of AMF+CHT NPs represents a sustainable and eco-friendly strategy for improving the EO quantity and quality of thyme under water stress conditions. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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16 pages, 2558 KiB

Open AccessArticle

Influence of Different Precursors on Content of Polyphenols in Camellia sinensis In Vitro Callus Culture

by Maria A. Aksenova, Tatiana L. Nechaeva, Maria Y. Zubova, Evgenia A. Goncharuk, Varvara V. Kazantseva, Vera M. Katanskaya, Petr V. Lapshin and Natalia V. Zagoskina

Plants 2023, 12(4), 796; https://doi.org/10.3390/plants12040796 - 10 Feb 2023

Cited by 2 | Viewed by 1498

Abstract

Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search [...] Read more.

Plant tissue cultures are considered as potential producers of biologically active plant metabolites, which include various phenolic compounds that can be used to maintain human health. Moreover, in most cases, their accumulation is lower than in the original explants, which requires the search for factors and influences for the intensification of this process. In this case, it is very promising to use the precursors of their biosynthesis as potential “regulators” of the various metabolites’ formation. The purpose of our research was to study the effect of L-phenylalanine (PhA, 3 mM), trans-cinnamic acid (CA, 1 mM) and naringenin (NG, 0.5 mM), as components of various stages of phenolic metabolism, on accumulation of various phenolic compound classes, including phenylpropanoids, flavans and proanthocyanidins, as well as the content of malondialdehyde in in vitro callus culture of the tea plant (Camellia sinensis L.). According to the data obtained, the precursors’ influence did not lead to changes in the morphology and water content of the cultures. At the same time, an increase in the total content of phenolic compounds, as well as phenylpropanoids, flavans and proanthocyanidins, was noted in tea callus cultures. Effectiveness of precursor action depends on its characteristics and the exposure duration, and was more pronounced in the treatments with PhA. This compound can be considered as the most effective precursor regulating phenolic metabolism, contributing to a twofold increase in the total content of phenolic compounds, flavanes and proanthocyanidins, and a fourfold increase in phenylpropanoids in tea callus cultures. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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16 pages, 2641 KiB

Open AccessArticle

Growth-Promoting Characteristics of Fungal and Bacterial Endophytes Isolated from a Drought-Tolerant Mint Species Endostemon obtusifolius (E. Mey. ex Benth.) N. E. Br

by Abdulazeez A. Ogbe, Shubhpriya Gupta, Wendy A. Stirk, Jeffrey F. Finnie and Johannes Van Staden

Plants 2023, 12(3), 638; https://doi.org/10.3390/plants12030638 - 01 Feb 2023

Cited by 7 | Viewed by 2062

Abstract

Endophytes are primarily endosymbiotic bacteria and fungi that colonize the interior tissues of their host plant. They enhance the host plant’s growth and attenuate adverse effects of biological stress. Endophytic species of many indigenous plants are an untapped resource of plant growth-promoting microorganisms [...] Read more.

Endophytes are primarily endosymbiotic bacteria and fungi that colonize the interior tissues of their host plant. They enhance the host plant’s growth and attenuate adverse effects of biological stress. Endophytic species of many indigenous plants are an untapped resource of plant growth-promoting microorganisms that can mitigate abiotic stress effects. Thus, this study aimed to isolate endophytes from the roots and leaves of the medicinal plant Endostemon obtusifolius to evaluate their in vitro growth-promoting capacities and drought tolerance and to characterize the most promising species. Twenty-six endophytes (fourteen bacteria and twelve fungi) were isolated and cultured from the roots and leaves of E. obtusifolius. All 26 endophytes produced flavonoids, and 14 strains produced phenolic compounds. Of the 11 strains that displayed good free radical scavenging capability (low IC₅₀) in the 1-1-diphenyl-1-picryhydrazyl radical scavenging assay, only three strains could not survive the highest drought stress treatment (40% polyethylene glycol). These 11 strains were all positive for ammonia and siderophore production and only one strain failed to produce hydrogen cyanide and solubilize phosphate. Seven isolates showed aminocyclopropane-1-carboxylate deaminase activity and differentially synthesized indole-3-acetic acid. Using molecular tools, two promising symbiotic, drought stress tolerant, and plant growth-enhancing endophytic species (EORB-2 and EOLF-5) were identified as Paenibacillus polymyxa and Fusarium oxysporum. The results of this study demonstrate that P. polymyxa and F. oxysporum should be further investigated for their drought stress mitigation and plant growth enhancement effects as they have the potential to be developed for use in sustainable agricultural practices. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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18 pages, 331 KiB

Open AccessArticle

Biomass, Essential Oil Yield, and Composition of Marjoram as Influenced by Interactions of Different Agronomic Practices under Controlled Conditions

by Mantwampe Johleen Malaka, Nadia Alcina Araya, Puffy Soundy, Christian Phillipus du Plooy, Hintsa Tesfamicael Araya, Willem Sternburg Jansen Van Rensburg, Eric Watkinson, Ellis Levember, Ebrahim Wadiwala and Stephen Oluwaseun Amoo

Plants 2023, 12(1), 173; https://doi.org/10.3390/plants12010173 - 30 Dec 2022

Cited by 3 | Viewed by 1685

Abstract

Origanum marjorana L. has been valued for centuries for its flavoring attributes and therapeutic properties. The growing demand for its various applications necessitates optimizing agronomic practices for its production. A glasshouse pot trial was conducted to identify optimum agronomic practices for increased herbage [...] Read more.

Origanum marjorana L. has been valued for centuries for its flavoring attributes and therapeutic properties. The growing demand for its various applications necessitates optimizing agronomic practices for its production. A glasshouse pot trial was conducted to identify optimum agronomic practices for increased herbage and oil yield, as well as oil quality. The effects of varying air temperature regimes (low, medium, and high levels), irrigation (low, medium, and high levels), nitrogen fertilizer application (N = 100, 150, and 200 kg/ha), and soil type (sandy loam, sandy clay loam, and loamy sand) on the productivity of marjoram plants were investigated. The results showed an increase in plant growth and herbage yield as well as chlorophyll content under conditions of high air temperature, low irrigation, and moderate to high nitrogen level applied to sandy loam soil, with an increase in oil yield with loamy sand soil. The major compounds observed in marjoram essential oil were terpinene-4-ol (22.63–36.72%) and (Z)-β-terpineol (6.85–16.60%), in which terpinene-4-ol was not found to be within the International Organization for Standardization (ISO) limits of acceptability while (Z)-β- terpineol had no reference limits available. A promising performance of marjoram cultivation under high regimes of air temperature (16.7 to 36.6 °C), nitrogen fertilization (200 kg ha⁻¹ N), and low irrigation (up to 60% soil water depletion from field capacity) on sandy loam soils was demonstrated for improved crop productivity. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

26 pages, 4766 KiB

Open AccessArticle

Optimizing Antioxidant Activity and Phytochemical Properties of Peppermint (Mentha piperita L.) by Integrative Application of Biofertilizer and Stress-Modulating Nanoparticles under Drought Stress Conditions

by Ali Ostadi, Abdollah Javanmard, Mostafa Amani Machiani and Karim Kakaei

Plants 2023, 12(1), 151; https://doi.org/10.3390/plants12010151 - 28 Dec 2022

Cited by 10 | Viewed by 2644

Abstract

Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO₂ NPs [...] Read more.

Drought stress (DS) negatively affects plant growth, productivity, and quality in semi-arid and arid regions. Nowadays, application of biofertilizers and stress-modulating nanoparticles (NPs) improves plant performance under stressful conditions. The study evaluated the impacts of arbuscular mycorrhizal fungi (Myco-Root) and TiO₂ NPs on the nutrient uptake, dry yield, essential oil (EO) productivity, and EO quality of peppermint (Mentha piperita L.) under different irrigation regimes. The treatments included three irrigation regimes containing irrigation after 20% (I₂₀, well-watered), 40% (I₄₀, mild DS), and 60% (I₆₀, severe DS) maximum allowable depletion (MAD) percentage of the soil’s available water as well as four fertilizer sources contain no fertilization (control), Myco-Root biofertilizer, TiO₂ NPs, and an integrative application of Myco-Root + TiO₂ NPs. The results demonstrated that the highest (195.72 g m⁻²) and the lowest dry yield (78.76 g m⁻²) of peppermint was obtained in well-watered conditions with integrative application of Myco-Root + TiO₂ NPs and severe drought stress (I₆₀) without fertilization, respectively. The dry yield of peppermint was reduced by 27.7 and 53.4% in mild (I₄₀) and severe drought stress (I₆₀), respectively. The maximum EO content (1.49%) and EO yield (2.30 g m⁻²) was recorded in mild drought stress (I₄₀) treated with Myco-Root + TiO₂ NPs. Based on the GC-MS and GC-FID analysis, 29 constituents were identified in peppermint EO, with the major constituents being menthol (38.99–52%), menthone (12.72–20.13%), 1,8-cineole (6.55–7.84%), and neo-menthol (3.14–4.52%), respectively. The maximum content of menthol, 1,8-cineole, and neo-menthol was obtained under mild drought stress (I₄₀) fertilized with Myco-Root + TiO₂ NPs. The results indicate that the integrative application of Myco-Root + TiO₂ NPs could be used as an alternative method of using chemical fertilizers in sustainable agricultural systems for improving the EO quantity and quality of peppermint grown under drought stress conditions. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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15 pages, 1783 KiB

Open AccessArticle

Improvement of German Chamomile (Matricaria recutita L.) for Mechanical Harvesting, High Flower Yield and Essential Oil Content Using Physical and Chemical Mutagenesis

by Yasser E. Ghareeb, Said S. Soliman, Tarek A. Ismail, Mohammed A. Hassan, Mohammed A. Abdelkader, Arafat Abdel Hamed Abdel Latef, Jameel M. Al-Khayri, Salha M. ALshamrani, Fatmah A. Safhi, Mohamed F. Awad, Diaa Abd El-Moneim and Abdallah A. Hassanin

Plants 2022, 11(21), 2940; https://doi.org/10.3390/plants11212940 - 01 Nov 2022

Cited by 9 | Viewed by 2363

Abstract

Chamomile (Matricariarecutita L.) is one of the most important medicinal plants with various applications. The flowers and flower heads are the main organs inthe production of essential oil. The essential improvement goals of chamomile are considered to be high flower yield and [...] Read more.

Chamomile (Matricariarecutita L.) is one of the most important medicinal plants with various applications. The flowers and flower heads are the main organs inthe production of essential oil. The essential improvement goals of chamomile are considered to be high flower yield and oil content, as well asthe suitability for mechanical harvesting. The present study aimed to improve the flower yield, oil content and mechanical harvestability of German chamomile via chemical and physical mutagens. Three German chamomile populations (Fayum, Benysuif and Menia) were irradiated with 100, 200, 300 and 400 Gray doses of gamma rays, as well as chemically mutagenized using 0.001, 0.002 and 0.003 mol/mL of sodium azide for 4 h. The two mutagens produced a wide range of changes in the flowers’ shape and size. At M₃ generation, 18 mutants (11 from gamma irradiation and 7 from sodium azide mutagenization) were selected and morphologically characterized. Five out of eighteen mutants were selected for morphological and chemical characterization for oil content, oil composition and oil quality in M₄ generation. Two promising mutants, F/LF5-2-1 and B/HNOF 8-4-2, were selected based on their performance in most studied traits during three generations, as well as the high percentage of cut efficiency and a homogenous flower horizon, which qualify them as suitable candidates for mechanical harvesting. The two mutants are late flowering elite mutants; the F/LF5-2-1 mutant possessed the highest oil content (1.77%) and number of flowers/plant (1595), while the second promising B/HNOF 8-4-2 mutant hada high oil content (1.29%) and chamazulene percentage (13.98%) compared to control plants. These results suggest that the B/HNOF 8-4-2 and F/LF5-2-1 mutants could be integrated as potential parents into breeding programs for a high number of flowers, high oil content, oil composition and oil color traits for German chamomile improvement. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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14 pages, 1514 KiB

Open AccessArticle

NADES Compounds Identified in Hypoxis hemerocallidea Corms during Dormancy

by Motiki M. Mofokeng, Gerhard Prinsloo, Hintsa T. Araya, Stephen O. Amoo, Christian P. du Plooy and Phatu W. Mashela

Plants 2022, 11(18), 2387; https://doi.org/10.3390/plants11182387 - 13 Sep 2022

Cited by 1 | Viewed by 1319

Abstract

Soaking Hypoxis hemerocallidea corms in distilled water improved the propagation and development of cormlets, suggesting the potential leaching-out of inhibitory chemical compounds. To investigate the presence of inhibitory compounds, nuclear magnetic resonance (NMR) spectral data of the leachate from dormant H. hemerocallidea corms [...] Read more.

Soaking Hypoxis hemerocallidea corms in distilled water improved the propagation and development of cormlets, suggesting the potential leaching-out of inhibitory chemical compounds. To investigate the presence of inhibitory compounds, nuclear magnetic resonance (NMR) spectral data of the leachate from dormant H. hemerocallidea corms were obtained using a 600 MHz ¹H-NMR spectrometer. The ¹H-NMR analysis led to the identification of choline, succinate, propylene glycol, and lactose, as inhibitory compounds. These four chemical compounds are part of the “Natural Deep Eutectic Solvents” (NADES) that protect plant cells during stress periods, each of which has the potential to inhibit bud growth and development. These compounds are supposedly leached out of the corms during the first rain under natural conditions, possibly accompanied by changes in the ratios of dormancy-breaking phytohormones and inhibitory compounds, to release bud dormancy. The identified chemical compounds heralded a novel frontier in the vegetative propagation of H. hemerocallidea as a medicinal plant, and for its enhanced sustainable uses. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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Open AccessReview

Propagation of Medicinal Plants for Sustainable Livelihoods, Economic Development, and Biodiversity Conservation in South Africa

by Olufunke O. Fajinmi, Olaoluwa O. Olarewaju and Johannes Van Staden

Plants 2023, 12(5), 1174; https://doi.org/10.3390/plants12051174 - 03 Mar 2023

Cited by 1 | Viewed by 2999

Abstract

South Africa is blessed with vast plant resources and unique vegetation types. Indigenous South African medicinal plants have been well-harnessed to generate income in rural communities. Many of these plants have been processed into natural products to heal a variety of diseases, making [...] Read more.

South Africa is blessed with vast plant resources and unique vegetation types. Indigenous South African medicinal plants have been well-harnessed to generate income in rural communities. Many of these plants have been processed into natural products to heal a variety of diseases, making them valuable export commodities. South Africa has one of the most effective bio-conservation policies in Africa, which has protected the South African indigenous medicinal vegetation. However, there is a strong link between government policies for biodiversity conservation, the propagation of medicinal plants as a source of livelihood, and the development of propagation techniques by research scientists. Tertiary institutions nationwide have played a crucial role in the development of effective propagation protocols for valuable South African medicinal plants. The government-restricted harvest policies have also helped to nudge natural product companies and medicinal plant marketers to embrace the cultivated plants for their medicinal uses, and thus have helped support the South African economy and biodiversity conservation. Propagation methods used for the cultivation of the relevant medicinal plants vary according to plant family and vegetation type, among others. Plants from the Cape areas, such as the Karoo, are often resuscitated after bushfires, and propagation protocols mimicking these events have been established through seed propagation protocols with controlled temperatures and other conditions, to establish seedlings of such plants. Thus, this review highlights the role of the propagation of highly utilized and traded medicinal plants in the South African traditional medicinal system. Some valuable medicinal plants that sustain livelihoods and are highly sought-after as export raw materials are discussed. The effect of South African bio-conservation registration on the propagation of these plants and the roles of the communities and other stakeholders in the development of propagation protocols for highly utilized and endangered medicinal plants are also covered. The role of various propagation methods on the bioactive compounds’ composition of medicinal plants and issues of quality assurance are addressed. The available literature, media online news, newspapers, and other resources, such as published books and manuals, were scrutinized for information. Full article

(This article belongs to the Special Issue Propagation and Cultivation of Medicinal Plants)

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