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Plants
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The Molecular Biology of Plant Cells
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The Molecular Biology of Plant Cells

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: closed (31 July 2023) | Viewed by 2449

Special Issue Editor

Dr. Chris Helliwell

E-Mail Website
Guest Editor
CSIRO Agriculture and Food, Canberra, ACT 2601, Australia
Interests: plant development; flowering; epigenetics; brassica crops; RNAi; gene editing

Special Issue Information

Dear Colleagues,

This Special Issue is focused on research that adds to our understanding of the molecular and biochemical mechanisms which gives rise to the properties of plant cells. The scope of this SI includes the specialization of plant cells, cell–cell interactions, and responses to the environment. Studies on both model plants and crop species are welcome, as are review articles in this area.

Dr. Chris Helliwell
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • genetics
  • gene expression
  • epigenetics
  • RNA biology
  • protein function

Published Papers (1 paper)

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Research

11 pages, 2047 KiB  
Article
CRISPR/Cas9 Based Cell-Type Specific Gene Knock-Out in Arabidopsis Roots
by Meng Li, Xufang Niu, Shuang Li, Shasha Fu, Qianfang Li, Meizhi Xu, Chunhua Wang and Shuang Wu
Plants 2023, 12(12), 2365; https://doi.org/10.3390/plants12122365 - 19 Jun 2023
Viewed by 2159
Abstract
CRISPR/Cas9 (hereafter Cas9)-mediated gene knockout is one of the most important tools for studying gene function. However, many genes in plants play distinct roles in different cell types. Engineering the currently used Cas9 system to achieve cell-type-specific knockout of functional genes is useful [...] Read more.
CRISPR/Cas9 (hereafter Cas9)-mediated gene knockout is one of the most important tools for studying gene function. However, many genes in plants play distinct roles in different cell types. Engineering the currently used Cas9 system to achieve cell-type-specific knockout of functional genes is useful for addressing the cell-specific functions of genes. Here we employed the cell-specific promoters of the WUSCHEL RELATED HOMEOBOX 5 (WOX5), CYCLIND6;1 (CYCD6;1), and ENDODERMIS7 (EN7) genes to drive the Cas9 element, allowing tissue-specific targeting of the genes of interest. We designed the reporters to verify the tissue-specific gene knockout in vivo. Our observation of the developmental phenotypes provides strong evidence for the involvement of SCARECROW (SCR) and GIBBERELLIC ACID INSENSITIVE (GAI) in the development of quiescent center (QC) and endodermal cells. This system overcomes the limitations of traditional plant mutagenesis techniques, which often result in embryonic lethality or pleiotropic phenotypes. By allowing cell-type-specific manipulation, this system has great potential to help us better understand the spatiotemporal functions of genes during plant development. Full article
(This article belongs to the Special Issue The Molecular Biology of Plant Cells)
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