Stars in Plant Cell Biology

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Cell Biology".

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 11419

Special Issue Editors


E-Mail Website
Guest Editor
Centre for Plant Science, School of Biology, University of Leeds, Leeds LS2 9JT, UK
Interests: plant development; cell wall biophysics; cell–cell transport and callose: B-1,3 glucans; plasmodesmata
Special Issues, Collections and Topics in MDPI journals
Department of Animal and Plant Sciences, University of Sheffield, Western Bank S10 2TN, UK
Interests: plant genetics and development; cell–cell signalling; receptor-like kinases; fertilisation; kinesins; cell division

Special Issue Information

Dear Colleagues,

Understanding how plant cells function continues to be an exciting and rapidly evolving field. Processes occurring at the cellular level control plant development, adaptation, environmental and physiological responses at the whole organism level. Progress has recently been made in dissecting plant cell structures, cell properties, cell environmental responses, developmental transitions and mechanisms of intercellular signalling, but many questions remain unanswered. Plant cell biology is increasingly interdisciplinary, combining insights from molecular biology, biochemistry, physiology, biophysics, computational biology and mathematics. As with all scientific areas, the plant cell biology community could benefit from widening participation of minority and low-represented groups to promote a further diversity of ideas.

In this Special Issue on “Stars in Plant Cell Biology”, we wish to highlight the contributions of early-career researchers and underrepresented minorities to this fascinating field. We invite research articles, short communications, and reviews on all aspects of plant cell biology of model or non-model systems. We welcome submissions from authors that identify with at least one of these three groups; 1) working in countries underrepresented in scientific publishing; 2) an early-career researcher (recently awarded PhD, postdoc, or within 5 years of first grant or first independent position); or 3) an underrepresented minority in your host country or in cell biology. Scientific excellence, research quality and significance will still be the main criteria for considering papers. Accepted papers will be published in an open access format and will attract a significant reduction in article processing charges.

Dr. Yoselin Benitez-Alfonso
Dr. Lisa Smith
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (2 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

17 pages, 1486 KiB  
Article
Optimizing Protocols for Arabidopsis Shoot and Root Protoplast Cultivation
by Taras Pasternak, Ivan A. Paponov and Serhii Kondratenko
Plants 2021, 10(2), 375; https://doi.org/10.3390/plants10020375 - 15 Feb 2021
Cited by 12 | Viewed by 7417
Abstract
Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant growth, plating efficiency, and promotion of shoot [...] Read more.
Procedures for the direct regeneration of entire plants from a shoot and root protoplasts of Arabidopsis thaliana have been optimized. The culture media for protoplast donor-plant cultivation and protoplast culture have been adjusted for optimal plant growth, plating efficiency, and promotion of shoot regeneration. Protocols have been established for the detection of all three steps in plant regeneration: (i) chromatin relaxation and activation of auxin biosynthesis, (ii) cell cycle progression, and (iii) conversion of cell-cycle active cells to totipotent ones. The competence for cell division was detected by DNA replication events and required high cell density and high concentrations of the auxinic compound 2,4-D. Cell cycle activity and globular structure formation, with subsequent shoot induction, were detected microscopically and by labeling with fluorescent dye Rhodamine123. The qPCR results demonstrated significantly upregulated expression of the genes responsible for nuclear reorganization, auxin responses, and auxin biosynthesis during the early stage of cell reprogramming. We further optimized cell reprogramming with this protocol by applying glutathione (GSH), which increases the sensitivity of isolated mesophyll protoplasts to cell cycle activation by auxin. The developed protocol allows us to investigate the molecular mechanism of the de-differentiation of somatic plant cells. Full article
(This article belongs to the Special Issue Stars in Plant Cell Biology)
Show Figures

Graphical abstract

17 pages, 4128 KiB  
Article
Wheat, Rye, and Barley Genomes Can Associate during Meiosis in Newly Synthesized Trigeneric Hybrids
by María-Dolores Rey, Carmen Ramírez and Azahara C. Martín
Plants 2021, 10(1), 113; https://doi.org/10.3390/plants10010113 - 07 Jan 2021
Cited by 5 | Viewed by 2983
Abstract
Polyploidization, or whole genome duplication (WGD), has an important role in evolution and speciation. One of the biggest challenges faced by a new polyploid is meiosis, in particular, discriminating between multiple related chromosomes so that only homologs recombine to ensure regular chromosome segregation [...] Read more.
Polyploidization, or whole genome duplication (WGD), has an important role in evolution and speciation. One of the biggest challenges faced by a new polyploid is meiosis, in particular, discriminating between multiple related chromosomes so that only homologs recombine to ensure regular chromosome segregation and fertility. Here, we report the production of two new hybrids formed by the genomes of species from three different genera: a hybrid between Aegilops tauschii (DD), Hordeum chilense (HchHch), and Secale cereale (RR) with the haploid genomic constitution HchDR (n = 7× = 21); and a hybrid between Triticum turgidum spp. durum (AABB), H. chilense, and S. cereale with the constitution ABHchR (n = 7× = 28). We used genomic in situ hybridization and immunolocalization of key meiotic proteins to establish the chromosome composition of the new hybrids and to study their meiotic behavior. Interestingly, there were multiple chromosome associations at metaphase I in both hybrids. A high level of crossover (CO) formation was observed in HchDR, which shows the possibility of meiotic recombination between the different genomes. We succeeded in the duplication of the ABHchR genome, and several amphiploids, AABBHchHchRR, were obtained and characterized. These results indicate that recombination between the genera of three economically important crops is possible. Full article
(This article belongs to the Special Issue Stars in Plant Cell Biology)
Show Figures

Figure 1

Back to TopTop