Molecular Breeding and Germplasm Improvement of Rice—2nd Edition

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 862

Special Issue Editors


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Guest Editor
China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
Interests: genetic improvement of rice quality; genetic improvement of rice quality; developmental biology of rice
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
Interests: rice; yield; quantitative trait locus
Special Issues, Collections and Topics in MDPI journals
China National Center for Rice Improvement and State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
Interests: rice; heading date; photoperiod sensitivity; quantitative trait locus; map-based cloning; functional analysis
Special Issues, Collections and Topics in MDPI journals

E-Mail
Guest Editor
China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China
Interests: rice quality; rice genetics and breeding; physical and chemical properties of rice
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, gene editing, whole-genome selection, synthetic biology frontier biotechnology and information technology such as artificial intelligence and big data have been integrated into conventional breeding techniques, breeding technology and intelligent design system, which continuously drives the modern breeding technology change iterations and has a significant influence on the crops of rice breeding. In addition, germplasm resources are the chip of crop breeding and every breakthrough in crop breeding is inseparable from the innovation of important germplasm.

In order to promote academic exchange on the latest trends and achievements of molecular breeding and germplasm improvement of rice, this Special Issue will focus on the promotion of the joint discussion of the latest research dynamics and directions, and find opportunities for collaboration in this field. All original research papers and reviews are welcome for submission to this Special Issue.

Contributions in this Special Issue may include, but are not limited to, the research and development of new technologies for rice molecular breeding and germplasm innovation. Particularly welcome is research on rice molecular breeding technologies such as gene editing, whole-genome selection, synthetic biology, intelligent design breeding and advances in the innovation of new rice germplasm with high yield, high quality, multiresistance, safety and high efficiency.

Dr. Xiangjin Wei
Dr. Yingxin Zhang
Dr. Weixun Wu
Dr. Guiai Jiao
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • rice molecular breeding
  • innovation of rice germplasm
  • gene editing
  • whole-genome selection
  • synthetic biology
  • intelligent design breeding

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Published Papers (1 paper)

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Research

13 pages, 2652 KiB  
Article
A Novel Allele in the Promoter of Wx Decreases Gene Expression and Confers Lower Apparent Amylose Contents in Japonica Rice (Oryza sativa L.)
by Weijie Tang, Haiyuan Chen, Suobing Zhang, Jun Tang, Jing Lin, Xianwen Fang, Gaoming Chen and Yunhui Zhang
Plants 2024, 13(5), 745; https://doi.org/10.3390/plants13050745 - 6 Mar 2024
Viewed by 647
Abstract
Wx is the key gene that controls amylose content (AC), and various alleles have been found in rice populations. Wxb is the major allele in japonica and produces moderate AC (15~18%). It was recently found that editing the promoter of Wx could [...] Read more.
Wx is the key gene that controls amylose content (AC), and various alleles have been found in rice populations. Wxb is the major allele in japonica and produces moderate AC (15~18%). It was recently found that editing the promoter of Wx could produce a series of alleles that have different Wx activities. Although some studies have edited the promoter, few studies have focused on the natural variations in Wx. Here, we used the Rice3K database to investigate variations in the Wx promoter and found that the allele Wx1764178 (A/G) has a higher LD (linkage disequilibrium) with the two key SNPs (1765751, T/G; 1768006, A/C), which could produce different Wx alleles and influence AC, as reported previously. Further study showed that the Wx1764178 allele (A/G) is functional and influences the expression of Wx positively. Editing the A allele using CRISPR‒Cas9 produced 36 and 3 bp deletions and caused a decrease in the expression of Wx. The apparent amylose content (AAC) in the edited lines was decreased by 7.09% and 11.50% compared with that of the wild type, which was the japonica variety Nipponbare with Wxb and the A allele at 1764178, while a complementary line with the G allele showed a lower AAC than the A allele with no effect on other agronomic traits. The AAC of the edited lines showed a higher increase than that of the wild type (Nipponbare, Wxb) in low-nitrogen conditions relative to high-nitrogen conditions. We also developed a dCAPS marker to identify the allele and found that the G allele has widely been used (82.95%) in japonica-bred varieties from Jiangsu Province, China. Overall, we found a functional allele (Wx1764178, A/G) in the Wx promoter that could affect AAC in japonica cultivars and be developed as markers for quality improvement in rice breeding programs. Full article
(This article belongs to the Special Issue Molecular Breeding and Germplasm Improvement of Rice—2nd Edition)
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Planned Papers

The below list represents only planned manuscripts. Some of these manuscripts have not been received by the Editorial Office yet. Papers submitted to MDPI journals are subject to peer-review.

Detection and Evaluation of Blast Resistance Genes in Backbone Indica Rice Parents from South China

Abstract: Rice blast caused by the pathogenic fungus Magnaporthe oryzae, poses a significant threat to rice cultivation. The identification of robust resistance germplasm is crucial for breeding resistant varieties. In this study, we employed functional molecular markers for 10 rice blast resistance genes, namely Pi1, Pi2, Pi5, Pi9, Pia, Pid2, Pid3, Pigm, Pikh, and Pita, to assess blast resistance across 91 indica rice backbone parents in South China. The results showed that a spectrum of resistance levels ranging from highly resistant (HR) to highly susceptible (HS), with corresponding frequencies of 0, 20, 40, 27, 16, and 0, respectively. Yearly correlations in blast resistance genes among the 91 indica rice backbone parents revealed: Pid2 (60.44%), Pia (50.55%), Pita (45.05%), Pi2 (32.97%), Pikh (4.4%), Pigm (2.2%), Pi9 (2.2%) and Pi1(1.1%). Significant variations were observed in the distribution frequencies of these 10 resistance genes among indica rice backbone parents across different provinces. Furthermore, as the number of aggregated resistance genes increased, parental resistance levels correspondingly improved, though the efficacy of different gene combinations varied significantly. This study provides the initial steps towards strategically distributing a variety of resistant indica rice genotypes across South China.

 

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