Special Issue "Trends in Plant Virus Epidemiology and Control: Opportunities from New or Improved Technologies"

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Protection and Biotic Interactions".

Deadline for manuscript submissions: 30 September 2023 | Viewed by 1193

Special Issue Editor

IFAPA, Sustainable Plant Protection, 04745 La Mojonera, Almeria, Spain
Interests: etiology of plant diseases; plant virus detection; epidemiology and control; virus–vector–plant relationships
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Plant viruses pose a continuous and serious constraint on both the yield and quality of crops worldwide. Globalization and climate change are also providing opportunities for new viruses to emerge and spread regularly, and thus food security is constantly challenged. Plant virus epidemiology studies the rates of temporal and spatial change of viral disease in populations, and determines factors underlying change. Here, transmission is a key determining factor and often depends on the interactions between viruses, host plants, and vectors. Developments in sequencing technology, nucleic acid amplification techniques and risk modelling approaches offer opportunities to confront these increasing risks. High-throughput sequencing-based surveillance approaches can provide information to better understand the impact of plant viruses on crops, supporting the development of more targeted field and lab-based diagnostic tools. Today, the control of viral disease involves novel integrated management strategies with new production practices, genetic markers for rapid screening for resistance, new resistant genes that are incorporated into existing cultivars or into new cultivars, vector management, and virus certification programs. CRISPR-based genome editing is used for generating resistance to a wide range of DNA and RNA viruses. Susceptibility genes are identified that will likely enhance the utility of CRISPR in making susceptible crops become resistant. This Special Issue of Plants will highlight novel techniques and methods used to improve our understanding of plant virus epidemics and to develop better ways to control these viruses.

Dr. Dirk Janssen
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant virus
  • epidemiology
  • modelling
  • vector
  • high-throughput sequencing
  • integrated disease management
  • resistance
  • crop breeding
  • CRISPR
  • susceptibility

Published Papers (1 paper)

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Research

Article
Development of an In-Field Real-Time LAMP Assay for Rapid Detection of Tomato Leaf Curl New Delhi Virus
Plants 2023, 12(7), 1487; https://doi.org/10.3390/plants12071487 - 29 Mar 2023
Viewed by 863
Abstract
Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions [...] Read more.
Tomato leaf curl New Delhi virus (ToLCNDV) represents a threat to economically important horticultural crops. A real-time loop-mediated isothermal amplification (LAMP) assay for in-field ToLCNDV detection was developed, coupled to a rapid sample preparation method, and tested both in field and laboratory conditions on zucchini squash, tomato, and pepper samples. A set of six LAMP primers was designed for specific ToCLNDV detection, targeting a 218-nucleotide sequence within the AV1 gene. The sensitivity, specificity and accuracy of the real-time LAMP assay and comparison with canonical PCR were evaluated. The real-time LAMP assay developed was about one-thousand times more sensitive than the conventional PCR method, detecting a total of 4.41 × 102 genome copies as minimum target; no cross-reactivity was detected with the other geminiviruses used as the outgroup. The rapid sample preparation method allows for a reliable detection with a low reaction delay (≈2–3 min) compared to canonical DNA extraction, providing results in less than 45 min. Lastly, an increase in ToLCNDV-positive sample detection was observed compared to PCR, in particular for asymptomatic plants (85% and 71.6%, respectively). The real-time LAMP assay developed is a rapid, simple, specific, and sensitive technique for ToLCNDV detection, and it can be adopted as a routine test, for both in-field and laboratory conditions. Full article
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