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Plants
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Biotechnology for Cotton Improvement
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Biotechnology for Cotton Improvement

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (20 December 2022) | Viewed by 5160

Special Issue Editors

Prof. Dr. Shuangxia Jin

E-Mail Website
Guest Editor
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
Interests: plant biotechnology; gene editing; plant genomics; cotton improvement
Special Issues, Collections and Topics in MDPI journals
Dr. Wei Gao

E-Mail Website
Guest Editor
State Key Laboratory of Cotton Biology, School of Life Sciences, Henan University, Kaifeng, China
Interests: plant biotechnology; cotton improvement; glandular structure; plant architecture
Prof. Dr. Xinhui Nie

E-Mail Website
Guest Editor
Key Laboratory of Oasis Ecology Agricultural of Xinjiang Bingtuan, Agricultural College, Shihezi University, Shihezi 832000, China
Interests: cotton biotechnology; cotton insect/disease control; cotton ideotype design; cotton molecular breeding

Special Issue Information

Dear Colleagues,

Cotton is one of the world’s important crops; it sustainably provides fiber, oil and edible protein. With the development of biotechnology and its application to cotton genome assembly, gene cloning, functional genomics, etc., the basic research and breeding process of cotton have been accelerated. New sequencing technologies have accelerated the revelation of the tetraploid cotton genome. New molecular markers and gene mapping methods accelerate the cloning of key genes associated with cotton yield and quality. The combination of innovations in cotton transformation and genome editing has facilitated the creation of new cotton germplasm. The emerging biotechnological tools play an important role in cotton improvement and the development of high-yielding and high-quality cotton varieties.

This Special Issue of Plants will broadly collect original manuscripts regarding the application of modern breeding biotechnologies in cotton, including cotton biotechnology, genome editing, bioinformatics research, molecular breeding, etc. We also welcome reviews on topics relevant to the contents above. Your generous submission and sharing will help drive cotton improvement.

Prof. Dr. Shuangxia Jin
Dr. Wei Gao
Prof. Dr. Xinhui Nie
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • cotton
  • fiber development
  • insect/disease control
  • cotton biotechnology
  • genome editing functional genomics
  • bioinformatics

Published Papers (2 papers)

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Research

19 pages, 2110 KiB  
Article
A New Method for Rapid Subcellular Localization and Gene Function Analysis in Cotton Based on Barley Stripe Mosaic Virus
by Weiwei Chen, Chaolin Huang, Chenmeng Luo, Yongshan Zhang, Bin Zhang, Zhengqing Xie, Mengyuan Hao, Hua Ling, Gangqiang Cao, Baoming Tian, Fang Wei and Gongyao Shi
Plants 2022, 11(13), 1765; https://doi.org/10.3390/plants11131765 - 01 Jul 2022
Cited by 2 | Viewed by 2187
Abstract
The difficulty of genetic transformation has restricted research on functional genomics in cotton. Thus, a rapid and efficient method for gene overexpression that does not rely on genetic transformation is needed. Virus-based vectors offer a reasonable alternative for protein expression, as viruses can [...] Read more.
The difficulty of genetic transformation has restricted research on functional genomics in cotton. Thus, a rapid and efficient method for gene overexpression that does not rely on genetic transformation is needed. Virus-based vectors offer a reasonable alternative for protein expression, as viruses can infect the host systemically to achieve expression and replication without transgene integration. Previously, a novel four-component barley stripe mosaic virus (BSMV) was reported to overexpress large fragments of target genes in plants over a long period of time, which greatly simplified the study of gene overexpression. However, whether this system can infect cotton and stably overexpress target genes has not yet been studied. In this study, we verified that this new BSMV system can infect cotton through seed imbibition and systemically overexpress large fragments of genes (up to 2340 bp) in cotton. The target gene that was fused with GFP was expressed at a high level in the roots, stems, and cotyledons of cotton seedlings, and stable fluorescence signals were detected in the cotton roots and leaves even after 4 weeks. Based on the BSMV overexpression system, the subcellular localization marker line of endogenous proteins localized in the nucleus, endoplasmic reticulum, plasma membrane, Golgi body, mitochondria, peroxisomes, tonoplast, and plastids were quickly established. The overexpression of a cotton Bile Acid Sodium Symporter GhBASS5 using the BSMV system indicated that GhBASS5 negatively regulated salt tolerance in cotton by transporting Na+ from underground to the shoots. Furthermore, multiple proteins were co-delivered, enabling co-localization and the study of protein–protein interactions through co-transformation. We also confirmed that the BSMV system can be used to conduct DNA-free gene editing in cotton by delivering split-SpCas9/sgRNA. Ultimately, the present work demonstrated that this BSMV system could be used as an efficient overexpression system for future cotton gene function research. Full article
(This article belongs to the Special Issue Biotechnology for Cotton Improvement)
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Graphical abstract

12 pages, 4187 KiB  
Communication
Genetic Mapping and Analysis of a Compact Plant Architecture and Precocious Mutant in Upland Cotton
by Lei Chao, Zhenyuan Pan, Jing Wang, Yuanlong Wu, Guangling Shui, Nurimanguli Aini, Binghui Tang, Chunping Guo, Peng Han, Panxia Shao, Xiaomin Tian, Xinyi Chang, Qiushuang An, Chunmei Ma, Chunyuan You, Longfu Zhu and Xinhui Nie
Plants 2022, 11(11), 1483; https://doi.org/10.3390/plants11111483 - 31 May 2022
Cited by 1 | Viewed by 1952
Abstract
With the promotion and popularization of machine cotton-picking, more and more attention has been paid to the selection of early-maturity varieties with compact plant architecture. The type of fruit branch is one of the most important factors affecting plant architecture and early maturity [...] Read more.
With the promotion and popularization of machine cotton-picking, more and more attention has been paid to the selection of early-maturity varieties with compact plant architecture. The type of fruit branch is one of the most important factors affecting plant architecture and early maturity of cotton. Heredity analysis of the cotton fruit branch is beneficial to the breeding of machine-picked cotton. Phenotype analysis showed that the types of fruit branches in cotton are controlled by a single recessive gene. Using an F2 population crossed with Huaxin102 (normal branch) and 04N-11 (nulliplex branch), BSA (Bulked Segregant Analysis) resequencing analysis and GhNB gene cloning in 04N-11, and allelic testing, showed that fruit branch type was controlled by the GhNB gene, located on chromosome D07. Ghnb5, a new recessive genotype of GhNB, was found in 04N-11. Through candidate gene association analysis, SNP 20_15811516_SNV was found to be associated with plant architecture and early maturity in the Xinjiang natural population. The GhNB gene, which is related to early maturity and the plant architecture of cotton, is a branch-type gene of cotton. The 20_15811516_SNV marker, obtained from the Xinjiang natural population, was used for the assisted breeding of machine-picked cotton varieties. Full article
(This article belongs to the Special Issue Biotechnology for Cotton Improvement)
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