Chromosome Manipulation for Crop Genomes

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: closed (31 May 2023) | Viewed by 1381

Special Issue Editors


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Guest Editor
Center for Informational Biology, School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China
Interests: plant molecular cell biology; plant chromosome; wheat genetics; wheat breeding; genomic evolution; chromosome engineering
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Guest Editor
College of Agronomy, Sichuan Agricultural University, Chengdu, China
Interests: wide hybridization; plant genomics; polyploid; wheat breeding; molecular cytogenetics

Special Issue Information

Dear Colleagues,

Traditional genetic and molecular marker analysis for plant germplasm conservation and evolutionary analysis limited their current applicability for crop improvement. The approaches to chromosome-manipulation-based molecular cytogenetic advances, including studies combined with fluorescence in situ hybridization and multi-omics arrays, bring genomic resources to the chromatin and gene level. Chromosome-based FISH experiments and oligo-based synthetic probes have thus become an attractive methodology for chromosome characterization and dissection in a large number of crop plants. This helps to overcome some of the shortcomings of traditional cytogenetic analysis in identifying potential cryptic alterations or reciprocal translocations. In the genomic era, novel technologies such as next-generation sequencing are now being used in different crops, including the polyploid and non-model crop species, for improving routine genetic dissection analysis and offering a great possibility of studying mutations and copy number alterations direct to breeding practices at a higher resolution, sensitivity, and efficiency.

This Special Issue aims to collect various research studies dealing with crop genetics and genomics that have relevance in chromosome manipulation, fluorescence in situ hybridization, genetic and genomic dissection for important traits, polyploidization, and gene expression in crops.

Prof. Dr. Zujun Yang
Prof. Dr. Zongxiang Tang
Guest Editors

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Keywords

  • genomics
  • chromatin
  • transcriptomics
  • cytogenetics
  • chromatin
  • polyploid
  • crop
  • translocation
  • evolution

Published Papers (1 paper)

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Research

14 pages, 6407 KiB  
Article
Molecular and Cytogenetic Identification of Wheat-Thinopyrum intermedium Double Substitution Line-Derived Progenies for Stripe Rust Resistance
by Guangrong Li, Qiheng Chen, Wenxi Jiang, Ahui Zhang, Ennian Yang and Zujun Yang
Plants 2023, 12(1), 28; https://doi.org/10.3390/plants12010028 - 21 Dec 2022
Cited by 2 | Viewed by 1158
Abstract
Thinopyrum intermedium (2n = 6x = 42, JJJSJSStSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat-Th. intermedium double [...] Read more.
Thinopyrum intermedium (2n = 6x = 42, JJJSJSStSt) has been hybridized extensively with common wheat and proven to be a valuable germplasm source for improving disease resistance and yield potential of wheat. A novel disease-resistant wheat-Th. intermedium double substitution line X479, carrying 1St(1B) and 4St-4JS (4B), was identified using multi-color non-denaturing fluorescence in situ hybridization (ND-FISH). With the aim of transferring Thinopyrum-specific chromatin to wheat, a total of 573 plants from F2 and F3 progenies of X479 crossed with wheat cultivar MY11 were developed and characterized using sequential ND-FISH with multiple probes. Fifteen types of wheat-Thinopyrum translocation chromosomes were preferentially transmitted in the progenies, and the homozygous wheat-1St, and wheat-4JSL translocation lines were identified using ND-FISH, Oligo-FISH painting and CENH3 immunostaining. The wheat-4JSL translocation lines exhibited high levels of resistance to stripe rust prevalent races in field screening. The gene for stripe rust resistance was found to be physically located on FL0–0.60 of the 4JSL, using deletion lines and specific DNA markers. The new wheat-Th. intermedium translocation lines can be exploited as useful germplasms for wheat improvement. Full article
(This article belongs to the Special Issue Chromosome Manipulation for Crop Genomes)
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