Formulations of Natural Origin and Synthetic Derivatives for the Treatment of Multi-Resistant Microorganisms

A special issue of Pharmaceutics (ISSN 1999-4923). This special issue belongs to the section "Physical Pharmacy and Formulation".

Deadline for manuscript submissions: closed (10 February 2022) | Viewed by 3830

Special Issue Editors


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Department of Pharmacy and Pharmacology, University of Bath, Bath BA2 7AY, UK
Interests: pharmaceutical analysis; natural products; electrospinning; electrospraying; biopolymers; coaxial; controlled drug delivery; nanofiber; polymer therapeutics; sustained release; tissue engineering; wound healing; biodegradable; gene therapy; polycaprolactone; drug metabolism and phytochemistry
Special Issues, Collections and Topics in MDPI journals

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Laboratorio de Productos Naturales y Síntesis Orgánica (LPNSO), Departamento de Química, Facultad de Ciencias Naturales y Exactas, Universidad de Playa Ancha, Avda. Leopoldo Carvallo 270, Playa Ancha, Valparaíso 2340000, Chile
Interests: chalcones; nanoemulsions; antimicrobials; anticancer

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Escuela de Obstetricia y Puericultura, Facultad de medicina, Campus de la Salud, Universidad de Valparaíso, Angamos 655, Reñaca, Viña del Mar 2520000, Chile
Interests: antibiotics; nanoformulations; biopesticides; natural products

Special Issue Information

Dear Colleagues,

Drug resistance by multidrug-resistant microorganisms (bacteria and fungi) is one of the priority problems for the World Health Organization, since they constitute a dilemma throughout the world due to their rapid dissemination, as well as the difficulty of their treatment. At present, there is a global trend towards a reduction in the use of current drugs that generate resistance, and, in their replacement, natural substances of low toxicity have been chosen. In this sense, phytopharmaceuticals based on natural products are shown to be an economically and socially viable alternative. Although there are many advantages in the use of natural products as new drugs, there are some problems that need to be solved. Indeed, nanotechnological applications for the control of multi-resistant microorganisms, such as nanoformulations, are one of the most novel and growing fields of research with the potential to solve these problems. This Special Issue aims to compile cutting-edge research and review papers illustrating the potential of extracts and active principles obtained from plants, as well as their derivatives, against multidrug-resistant microorganisms, with a special focus on new delivery systems. In addition, this issue will focus particularly on aspects of design, development, manufacturing, characterization and modeling of formulations for pharmaceutical application.

Dr. Ian S. Blagbrough
Dr. Alejandro Martín Madrid Villegas
Dr. Iván Jose Montenegro Venegas
Guest Editors

Manuscript Submission Information

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Keywords

  • drug design
  • natural products
  • biopharmaceutics
  • nanoemulsion
  • nanotechnology
  • botanical drug
  • phytonanotechnology
  • nanopharmacology
  • drug targeting
  • molecular biology

Published Papers (1 paper)

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Research

20 pages, 19490 KiB  
Article
Filamentous Fungi Producing l-Asparaginase with Low Glutaminase Activity Isolated from Brazilian Savanna Soil
by Marcela Freitas, Paula Souza, Samuel Cardoso, Kellen Cruvinel, Letícia Santos Abrunhosa, Edivaldo X. Ferreira Filho, João Inácio, Danilo Batista Pinho, Adalberto Pessoa and Pérola O. Magalhães
Pharmaceutics 2021, 13(8), 1268; https://doi.org/10.3390/pharmaceutics13081268 - 17 Aug 2021
Cited by 11 | Viewed by 3160
Abstract
l-asparaginase is an enzyme used as treatment for acute lymphoblastic leukemia (ALL) due to its ability to hydrolyze l-asparagine, an essential amino acid synthesized by normal cells unlike neoplastic cells. The adverse effects of l-asparaginase formulations are associated with its [...] Read more.
l-asparaginase is an enzyme used as treatment for acute lymphoblastic leukemia (ALL) due to its ability to hydrolyze l-asparagine, an essential amino acid synthesized by normal cells unlike neoplastic cells. The adverse effects of l-asparaginase formulations are associated with its glutaminase activity and bacterial origin; therefore, it is important to find new sources of l-asparaginase-producing eukaryotic microorganisms with low glutaminase activity. This work evaluated the biotechnological potential of filamentous fungi isolated from Brazilian Savanna soil and plants for l-asparaginase production. Thirty-nine isolates were screened for enzyme production using the plate assay, followed by measuring enzymatic activity in cells after submerged fermentation. The variables influencing l-asparaginase production were evaluated using Plackett–Burman design. Cell disruption methods were evaluated for l-asparaginase release. Penicillium sizovae 2DSST1 and Fusarium proliferatum DCFS10 showed the highest l-asparaginase activity levels and the lowest glutaminase activity levels. Penicillium sizovae l-asparaginase was repressed by carbon sources, whereas higher carbon concentrations enhanced l-asparaginase by F. proliferatum. Maximum enzyme productivity, specific enzyme yield and the biomass conversion factor in the enzyme increased after Plackett–Burman design. Freeze-grinding released 5-fold more l-asparaginase from cells than sonication. This study shows two species, which have not yet been reported, as sources of l-asparaginase with possible reduced immunogenicity for ALL therapy. Full article
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