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Pathogens
Special Issues
Advances in Plant Viruses
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Special Issue "Advances in Plant Viruses"

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Viral Pathogens".

Deadline for manuscript submissions: 31 December 2023 | Viewed by 507

Special Issue Editors

Dr. Kathleen Hefferon

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Guest Editor
Department of Cell and Systems Biology, Cornell University, Ithaca, NY 14850, USA
Interests: plant biotechnology; transgenic plant vaccine; plant virus nanoparticles for anti-cancer therapy
Special Issues, Collections and Topics in MDPI journals
Dr. Srividhya Venkataraman

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Guest Editor
Virology Laboratory, Department of Cell & Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada
Interests: virology; plant viruses; viroids; satellites; agricultural biotechnology; genetic engineering; food security; virus-like particles; viral nanoparticles
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent years, plant viruses have been detected in many environments and in the feces of various animals and insects. Emerging plant viruses cause considerable economic losses and threaten sustainable agriculture. Generally, the control of diseases derived from plant viruses is based on restraining virus dispersion. Although a variety of methods have been employed to study plant viruses, our surprising lack of knowledge about plant viruses indicates the need for more comprehensive studies.

This Special Issue aims to provide a platform for researchers interested in plant virology to share their recent findings. We invite you to submit research articles, short communications, or reviews relating to the various aspects of plant virology.

Dr. Kathleen Hefferon
Dr. Srividhya Venkataraman
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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Research

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Article
Optimization and Validation of Reverse Transcription Recombinase-Aided Amplification (RT-RAA) for Sorghum Mosaic Virus Detection in Sugarcane
by
Fenglin Wang
,
Qinmin Liang
,
Rongman Lv
,
Shakeel Ahmad
,
Mishal Bano
,
Guangzhen Weng
and
Ronghui Wen
Pathogens 2023, 12(8), 1055; https://doi.org/10.3390/pathogens12081055 - 18 Aug 2023
Viewed by 336
Abstract
Sorghum mosaic virus (SrMV) causes sugarcane mosaic disease and has significant adverse economic impacts on the cultivation of sugarcane. This study aimed to develop a rapid isotherm nucleic acid amplification method for detecting SrMV. Specific primers were designed to target the conserved region [...] Read more.
Sorghum mosaic virus (SrMV) causes sugarcane mosaic disease and has significant adverse economic impacts on the cultivation of sugarcane. This study aimed to develop a rapid isotherm nucleic acid amplification method for detecting SrMV. Specific primers were designed to target the conserved region of the P3 gene of SrMV. The reverse transcription recombinase-aided amplification (RT-RAA) method was developed by screening primers and optimizing reaction conditions. Comparative analyses with RT-PCR demonstrated that the RT-RAA method exhibited superior specificity, sensitivity, and reliability for SrMV detection. Notably, using a standard plasmid diluted 10-fold continuously as a template, the sensitivity of RT-RAA was 100-fold higher than that of RT-PCR. Moreover, the RT-RAA reaction displayed flexibility in a temperature range of 24–49 °C, eliminating the need for expensive and complex temperature control equipment. Thus, this method could be utilized at ambient or even human body temperature. Within a short duration of 10 min at 39 °C, the target sequence of SrMV could be effectively amplified. Specificity analysis revealed no cross-reactivity between SrMV and other common sugarcane viruses detected via the RT-RAA. With its high sensitivity, rapid reaction time, and minimal equipment requirements, this method presents a promising diagnostic tool for the reliable and expedited detection of SrMV. Furthermore, it indicates broad applicability for successfully detecting other sugarcane viruses. Full article
(This article belongs to the Special Issue Advances in Plant Viruses)
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