Rose Rosette Disease

A special issue of Pathogens (ISSN 2076-0817). This special issue belongs to the section "Viral Pathogens".

Deadline for manuscript submissions: closed (28 February 2023) | Viewed by 13315

Special Issue Editor

Department of Agriculture-Agricultural Research Service, Floral and Nursery Plants Research Unit, United States National Arboretum, Beltsville, MD, 20705, USA
Interests: plant viruses; virus detection, identification, and characterization; virus-host and virus-vector interactions; virus-induced gene silencing; viruses of ornamental plants

Special Issue Information

Dear Colleagues,

Rose Rosette Disease (RRD) is caused by infection of roses by rose rosette virus (RRV), (Rose rosette emaravirus, genus Emaravirus, family Fimoviridae). RRV is transmitted by the eriophyid mite Phyllocoptes fructiphilus, whereas other eriophyid mites colonizing roses are not known to transmit RRV. RRV is currently known only from North America, first identified in wild roses in the western mountains, later becoming established in the introduced multiflora rose, and currently causing widespread economic damage in cultivated roses.

For the forthcoming Special Issue of Pathogens, we invite you to submit research articles, review articles, short notes as well as communications related to RRD. The Special Issue will provide an overview of recent research including fundamental and applied studies, bringing together knowledge of the factors involved in: disease transmission and interactions between RRV, the eriophyid mite vector, and host plant genotype; screening, identification and utilization of germplasm resistant to the mite and/or RRV; development of diagnostic methods useful to producers, breeders, and  rose enthusiasts; factors affecting geographic distribution of RRV and the vector; potential means for disease management in production fields and urban/suburban/commercial landscapes, through chemical means, biocontrol, and improving crop tolerance or resistance; and the influence of mixed viral infections on disease severity. We look forward to your contribution.

Dr. John Hammond
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Pathogens is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Rose rosette virus
  • Emaravirus
  • Fimoviridae
  • Plant virus
  • epidemiology
  • virus vectors
  • plant-virus interactions
  • eriophyid mite-virus interactions
  • eriophyid mite-plant interactions
  • eriophyid mite phenology
  • resistance
  • biocontrol
  • integrated disease management
  • RNA silencing
  • genetics
  • genomics

Published Papers (6 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

24 pages, 14052 KiB  
Article
Genetic Diversity among Rose Rosette Virus Isolates: A Roadmap towards Studies of Gene Function and Pathogenicity
by Jeanmarie Verchot, Venura Herath, Ramon Jordan and John Hammond
Pathogens 2023, 12(5), 707; https://doi.org/10.3390/pathogens12050707 - 12 May 2023
Cited by 1 | Viewed by 1808
Abstract
The phylogenetic relationships of ninety-five rose rosette virus (RRV) isolates with full-length genomic sequences were analyzed. These isolates were recovered mostly from commercial roses that are vegetatively propagated rather than grown from seed. First, the genome segments were concatenated, and the maximum likelihood [...] Read more.
The phylogenetic relationships of ninety-five rose rosette virus (RRV) isolates with full-length genomic sequences were analyzed. These isolates were recovered mostly from commercial roses that are vegetatively propagated rather than grown from seed. First, the genome segments were concatenated, and the maximum likelihood (ML) tree shows that the branches arrange independent of their geographic origination. There were six major groups of isolates, with 54 isolates in group 6 and distributed in two subgroups. An analysis of nucleotide diversity across the concatenated isolates showed lower genetic differences among RNAs encoding the core proteins required for encapsidation than the latter genome segments. Recombination breakpoints were identified near the junctions of several genome segments, suggesting that the genetic exchange of segments contributes to differences among isolates. The ML analysis of individual RNA segments revealed different relationship patterns among isolates, which supports the notion of genome reassortment. We tracked the branch positions of two newly sequenced isolates to highlight how genome segments relate to segments of other isolates. RNA6 has an interesting pattern of single-nucleotide mutations that appear to influence amino acid changes in the protein products derived from ORF6a and ORF6b. The P6a proteins were typically 61 residues, although three isolates encoded P6a proteins truncated to 29 residues, and four proteins extended 76–94 residues. Homologous P5 and P7 proteins appear to be evolving independently. These results suggest greater diversity among RRV isolates than previously recognized. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

12 pages, 720 KiB  
Article
Meta-Analysis of Rose Rosette Disease-Resistant Quantitative Trait Loci and a Search for Candidate Genes
by Tessa Hochhaus, Jeekin Lau, Cristiane H. Taniguti, Ellen L. Young, David H. Byrne and Oscar Riera-Lizarazu
Pathogens 2023, 12(4), 575; https://doi.org/10.3390/pathogens12040575 - 08 Apr 2023
Viewed by 1937
Abstract
Rose rosette disease (RRD), caused by the rose rosette emaravirus (RRV), is a major viral disease in roses (Rosa sp.) that threatens the rose industry. Recent studies have revealed quantitative trait loci (QTL) for reduced susceptibility to RRD in the linkage groups [...] Read more.
Rose rosette disease (RRD), caused by the rose rosette emaravirus (RRV), is a major viral disease in roses (Rosa sp.) that threatens the rose industry. Recent studies have revealed quantitative trait loci (QTL) for reduced susceptibility to RRD in the linkage groups (LGs) 1, 5, 6, and 7 in tetraploid populations and the LGs 1, 3, 5, and 6 in diploid populations. In this study, we seek to better localize and understand the relationship between QTL identified in both diploid and tetraploid populations. We do so by remapping the populations found in these studies and performing a meta-analysis. This analysis reveals that the peaks and intervals for QTL using diploid and tetraploid populations co-localized on LG 1, suggesting that these are the same QTL. The same was seen on LG 3. Three meta-QTL were identified on LG 5, and two were discovered on LG 6. The meta-QTL on LG 1, MetaRRD1.1, had a confidence interval (CI) of 10.53 cM. On LG 3, MetaRRD3.1 had a CI of 5.94 cM. MetaRRD5.1 had a CI of 17.37 cM, MetaRRD5.2 had a CI of 4.33 cM, and MetaRRD5.3 had a CI of 21.95 cM. For LG 6, MetaRRD6.1 and MetaRRD6.2 had CIs of 9.81 and 8.81 cM, respectively. The analysis also led to the identification of potential disease resistance genes, with a primary interest in genes localized in meta-QTL intervals on LG 5 as this LG was found to explain the greatest proportion of phenotypic variance for RRD resistance. The results from this study may be used in the design of more robust marker-based selection tools to track and use a given QTL in a plant breeding context. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

13 pages, 650 KiB  
Article
Field Resistance to Rose Rosette Disease as Determined by Multi-Year Evaluations in Tennessee and Delaware
by Mark T. Windham, Thomas Evans, Sara Collins, Juniper A. Lake, Jeekin Lau, Oscar Riera-Lizarazu and David H. Byrne
Pathogens 2023, 12(3), 439; https://doi.org/10.3390/pathogens12030439 - 10 Mar 2023
Cited by 1 | Viewed by 2297
Abstract
Rose rosette disease (RRD) caused by the rose rosette emaravirus (RRV) and transmitted by the eriophyid mite Phyllocoptes fructiphilus (Pf), both native to North America, has caused significant damage to roses over the last several decades. As cultural and chemical control of [...] Read more.
Rose rosette disease (RRD) caused by the rose rosette emaravirus (RRV) and transmitted by the eriophyid mite Phyllocoptes fructiphilus (Pf), both native to North America, has caused significant damage to roses over the last several decades. As cultural and chemical control of this disease is difficult and expensive, a field trial was established to systematically screen rose germplasm for potential sources of resistance. One hundred and eight rose accessions representing the diversity of rose germplasm were planted in Tennessee and Delaware, managed to encourage disease development, and evaluated for symptom development and viral presence for three years. All major commercial rose cultivars were susceptible to this viral disease to varying levels. The rose accessions with no or few symptoms were species accessions from the sections Cinnamomeae, Carolinae, Bracteatae, and Systylae or hybrids with these. Among these, some were asymptomatic; they displayed no symptoms but were infected by the virus. Their potential depends on their ability to serve as a source of viruses. The next step is to understand the mechanism of resistance and genetic control of the various sources of resistance identified. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

14 pages, 2028 KiB  
Article
Exploring the Host Range of Rose rosette Virus among Herbaceous Annual Plants
by Osama O. Atallah, Sherin M. Yassin, Natalie Shirley and Jeanmarie Verchot
Pathogens 2022, 11(12), 1514; https://doi.org/10.3390/pathogens11121514 - 10 Dec 2022
Viewed by 1580
Abstract
To study the host range of Rose rosette virus (RRV), we employed crude sap inoculum extracted from RRV-infected roses and the RRV infectious clone. We inoculated plants from the families Solanaceae, Cucurbitaceae, Leguminosae, Malvaceae, Amaranthaceae, and Brassicaceae. Reverse [...] Read more.
To study the host range of Rose rosette virus (RRV), we employed crude sap inoculum extracted from RRV-infected roses and the RRV infectious clone. We inoculated plants from the families Solanaceae, Cucurbitaceae, Leguminosae, Malvaceae, Amaranthaceae, and Brassicaceae. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect RRV in the inoculated plants throughout their growth stages. Interestingly, RRV was detected in the newly developed leaves of tomato, pepper, tobacco, cucumber, squash, zucchini, pumpkin, pea, peanut, soybean, spinach, okra, and Chenopodium spp. The speed of upward advancement of RRV within infected plants was variable between plants as it took two to three weeks for some plant species and up to five weeks in other plant species to emerge in the newest leaves. No severe symptoms were detected on most of the inoculated plants. Chenopodium spp., spinach, cucumber and Nicotiana rustica exhibited either chlorotic or necrotic lesions with variable shapes and patterns on the systemically infected leaves. Double membrane-bound particles of 80–120 nm in diameter were detected by transmission electron microscopy in the infected tissues of cucumber, pepper, and N. benthamiana plants. This finding infers the validity of mechanical inoculation for RRV on a wide range of plants that would serve as potential natural reservoirs. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

20 pages, 3007 KiB  
Article
Identification of QTLs for Reduced Susceptibility to Rose Rosette Disease in Diploid Roses
by Ellen L. Young, Jeekin Lau, Nolan B. Bentley, Zena Rawandoozi, Sara Collins, Mark T. Windham, Patricia E. Klein, David H. Byrne and Oscar Riera-Lizarazu
Pathogens 2022, 11(6), 660; https://doi.org/10.3390/pathogens11060660 - 08 Jun 2022
Cited by 8 | Viewed by 2505
Abstract
Resistance to rose rosette disease (RRD), a fatal disease of roses (Rosa spp.), is a high priority for rose breeding. As RRD resistance is time-consuming to phenotype, the identification of genetic markers for resistance could expedite breeding efforts. However, little is known [...] Read more.
Resistance to rose rosette disease (RRD), a fatal disease of roses (Rosa spp.), is a high priority for rose breeding. As RRD resistance is time-consuming to phenotype, the identification of genetic markers for resistance could expedite breeding efforts. However, little is known about the genetics of RRD resistance. Therefore, we performed a quantitative trait locus (QTL) analysis on a set of inter-related diploid rose populations phenotyped for RRD resistance and identified four QTLs. Two QTLs were found in multiple years. The most consistent QTL is qRRV_TX2WSE_ch5, which explains approximately 20% and 40% of the phenotypic variation in virus quantity and severity of RRD symptoms, respectively. The second, a QTL on chromosome 1, qRRD_TX2WSE_ch1, accounts for approximately 16% of the phenotypic variation for severity. Finally, a third QTL on chromosome 3 was identified only in the multiyear analysis, and a fourth on chromosome 6 was identified in data from one year only. In addition, haplotypes associated with significant changes in virus quantity and severity were identified for qRRV_TX2WSE_ch5 and qRRD_TX2WSE_ch1. This research represents the first report of genetic determinants of resistance to RRD. In addition, marker trait associations discovered here will enable better parental selection when breeding for RRD resistance and pave the way for marker-assisted selection for RRD resistance. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

12 pages, 2084 KiB  
Article
Temporal Incidence of Eriophyid Mites on Rose Rosette Disease-Symptomatic and -Asymptomatic Roses in Central Georgia, USA
by Alejandra Monterrosa, Mathews L. Paret, Ronald Ochoa, Andrew Ulsamer and Shimat V. Joseph
Pathogens 2022, 11(2), 228; https://doi.org/10.3390/pathogens11020228 - 09 Feb 2022
Cited by 2 | Viewed by 1983
Abstract
Phyllocoptes fructiphilus Keifer (Acari: Eriophyidae) is the vector of rose rosette virus (RRV), which causes rose rosette disease (RRD) in North America. The RRD symptoms, such as witches’ broom, flower, and leaf deformation, disrupt the aesthetic appearance of plants and cause plant mortality. [...] Read more.
Phyllocoptes fructiphilus Keifer (Acari: Eriophyidae) is the vector of rose rosette virus (RRV), which causes rose rosette disease (RRD) in North America. The RRD symptoms, such as witches’ broom, flower, and leaf deformation, disrupt the aesthetic appearance of plants and cause plant mortality. Because there is no cure for RRV, it is critical to manage the vector and reduce the spread of the virus. The information on the phenology of P. fructiphilus on rose plants is essential to develop management strategies and reduce its spread. Thus, the objectives of the study were to determine 1) the phenology of eriophyid mites (including P. fructiphilus) in central Georgia due to its widespread occurrence in the state and 2) the incidence of eriophyid mites on closed and opened flower buds and other plant parts. In central Georgia, eriophyid mites, including P. fructiphilus were active on both symptomatic and asymptomatic plants from April to December. The mite densities were greater during July and August than during the remaining months on asymptomatic plants. The mites were more abundant on the RRD-symptomatic than on the asymptomatic plants. Similar numbers of eriophyid mites were observed on closed and opened flower buds. Eriophyid mite densities were greater on sepals and leaf bases than on other plant parts. Full article
(This article belongs to the Special Issue Rose Rosette Disease)
Show Figures

Figure 1

Back to TopTop