New Developments and Applications of Advanced Optical Microscopy. A Special Issue Derived from SPAOM 2018 (2nd Spanish Portuguese Meeting for Advanced Optical Microscopy)

A special issue of Methods and Protocols (ISSN 2409-9279).

Deadline for manuscript submissions: closed (28 February 2019) | Viewed by 346

Special Issue Editors

Department of Plant Stress, Development and Signaling, Estación Experimental del Zaidín, Profesor Albareda 1, E-18008 Granada, Spain
Interests: ROS/NO metabolism in plants; NO signaling in plant reproductive tissues; nitroproteomics; NO-derived PTM prediction in silico; NO–glutathione interaction in plants; effects of air pollutants on atmospheric pollen grains and spores; NO in allergy and other inflammatory disorders
Special Issues, Collections and Topics in MDPI journals
Dr. María Calvo Adamuz
Guest Editor
Advanced Optical Microscopy Unit, Scientific and Technological Centers, Universitat de Barcelona, Faculty of Medicine, Casanova 143, 5th floor (South Wing), E-08036 Barcelona, Spain
Advanced Light Microscopy Unit, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen, 208, 4200-135 Porto, Portugal

Special Issue Information

Dear Colleagues,

This Special Issue on “New Developments and Applications of Advanced Optical Microscopy” derives from SPAOM2018, the 2nd Spanish Portuguese Meeting for Advanced Optical Microscopy celebrated in Granada (Spain), 24–26 October 2018. We welcome contributions in the form of review articles and original research manuscripts, not restricted to participants of the meeting but also from interested field-related authors. Topics covered within the congress and also by the present volume include live optical imaging and analysis of all types of organisms (plants, animals, bacteria, humans, etc.). Imaging methods will be reviewed from using whole organisms to single molecules going through tissues and single cells. Therefore, multiscale, mesoscale, and nanoscale observations are considered. Methodological approaches include clearing, sample processing, labelling, visualization, tracking, high content screening, data mining, and machine learning. Descriptions and applications of instrumental adaptations and new technological developments are also welcome. Reports of imaging protocols and image analysis and improvement strategies and data curation are likewise encouraged.

Submitted articles should provide, as requested by the journal, background information, full experimental details, and comparison with available protocols/methods. The topic fits extremely well with the suggestion of the journal of submitting accompanying video demonstrations, which will be published online. The journal has no restriction on the length of the papers, and publication charges will be waived for the proposed Special Issue. Do not miss this opportunity to publicize your innovative work.

Dr. Juan de Dios Alché Ramírez
Dr. María Calvo Adamuz
Dr. Paula Sampaio
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • 4Pi Microscopy
  • Atomic Force microscopy (AFM)
  • Big data
  • Bioluminescence Imaging (BLI)
  • Brightfield & other Transmission Microscopy Contrasts
  • Correlated Light and Electron Microscopy (CLEM)
  • Data mining
  • Dyes design
  • Fluorescence Correlation Spectroscopy (FCS)
  • Fluorescence Lifetime Imaging Microscopy (FLIM)
  • Förster Resonance Energy Transfer (FRET)
  • High-Content Screening (HCS)
  • High Speed Confocal Laser Scanning Microscopy (CLSM)
  • Hyperspectral imaging
  • Image Analysis
  • Image Analysis/ Colocalization
  • Image Analysis/ Software
  • Image Analysis/Deconvolution
  • In vivo Imaging
  • Light Sheet Microscopy (SPIM, DSLM, DiSPIM)
  • Machine learning
  • Mesoscopic imaging
  • Multiscale imaging
  • Nonlinear microscopy (TPEF, SHG, THG)
  • Optical tweezers
  • Photoexcitation and photoablation
  • Single molecules detection
  • Super resolution microscopy (STED, STORM, PALM, Structured Illumination)
  • Tissue imaging

Published Papers

There is no accepted submissions to this special issue at this moment.
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