Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
6.7
4.6
Journals
Molecules
Special Issues
New Approaches in the Extraction of Biomolecules from Plants–Molecular and...
molecules-logo
Submit to Special Issue Submit Abstract to Special Issue Review for Molecules Propose a Special Issue

Journal Menu

► Journal Menu

Journal Browser

► Journal Browser
share announcement

New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 September 2024 | Viewed by 8517

Special Issue Editors

Prof. Dr. Sławomir Dresler

E-Mail Website
Guest Editor
1. Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a St., 20-093 Lublin, Poland
2. Department of Plant Physiology and Biophysics, Institute of Biological Science, Maria Curie-Skłodowska University, Akademicka 13 st., 20-033 Lublin, Poland
Interests: secondary metabolites in plants; interaction between environmental stress and secondary metabolites in plants; chemometrics; phytochemistry
Special Issues, Collections and Topics in MDPI journals
Prof. Dr. Maciej Strzemski

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Medical University of Lublin, Chodźki 4a St., 20-093 Lublin, Poland
Interests: natural deep eutectic solvents; green extractions; phytochemistry; ethnopharmacology; interaction between environmental stress and secondary metabolites in plants
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The extraction of secondary metabolites including flavonoids, phenolics, glycosides, terpene compounds, or nitrogenous compounds from plants is one of the most important steps in phytochemical research. Currently, numerous extraction methods and techniques are used; however, new approaches are constantly being developed to meet the new requirements and expectations. The modern approach to the extraction of plant material is targeted not only at the minimization of costs, but also simplicity, flexibility, and care for the natural environment. The process of miniaturization is also important, as it allows analyzing very small samples. The researchers' attention is therefore focused on the use of micro- and nano-extraction, limiting the consumption of toxic extractants through the appropriate design of experiments (DOE) and the use of non-toxic extractants such as supercritical carbon dioxide or natural deep eutectic solvents (NADES).

This Special Issue of Molecules will focus on new approaches in the extraction of biologically active molecules from plants and, in this context, the chemical and physical interactions between molecules and extracted constituents (liquid phase, solid phase, and gas phase). We welcome research and review articles that contribute to the following topics, among others:

  • Utilization of computation techniques in extraction (DOE);
  • NADES (natural deep eutectic solvents);
  • New equipment and techniques for extraction processes;
  • Micro- and nano-extraction;
  • Supercritical fluid extraction;
  • Enzyme-assisted extraction;
  • Theoretical background of "green extraction".

Prof. Dr. Sławomir Dresler
Prof. Dr. Maciej Strzemski
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant secondary metabolites
  • biologically active compounds
  • design of experiments
  • natural deep eutectic solvents
  • ionic liquids
  • micro- and nano-solid phase extraction
  • accelerated solvent extraction
  • supercritical fluid extraction
  • single-drop extraction

Published Papers (5 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

12 pages, 1664 KiB  
Article
Methodological Aspects of Green Extraction of Usnic Acid Using Natural Deep Eutectic Solvents
by Magdalena Kulinowska, Sławomir Dresler, Agnieszka Skalska-Kamińska, Agnieszka Hanaka and Maciej Strzemski
Molecules 2023, 28(14), 5321; https://doi.org/10.3390/molecules28145321 - 10 Jul 2023
Viewed by 1047
Abstract
Usnic acid (UA) is a compound with multiple biological activities that make it useful in various industries, e.g., pharmaceutical, cosmetic, dentistry, and agricultural sectors. Lichens are the primary source of UA, which is primarily extracted using acetone. This study aimed to investigate the [...] Read more.
Usnic acid (UA) is a compound with multiple biological activities that make it useful in various industries, e.g., pharmaceutical, cosmetic, dentistry, and agricultural sectors. Lichens are the primary source of UA, which is primarily extracted using acetone. This study aimed to investigate the solubility of UA in numerous natural deep eutectic solvents (NADESs) and use a mixture of thymol and camphor as a NADES in the optimization of the UA extraction process with the design of experiments method. For numerical optimization, the following parameters were employed in the experiment to confirm the model: a camphor-to-thymol ratio of 0.3, a liquid-to-solid ratio of 60, and a time of 30 min. The obtained experimental results aligned well with the predicted values, with the mean experimental value falling within the confidence interval, exhibiting deviations between 11.93 and 14.96. By employing this model, we were able to optimize the extraction procedure, facilitating the isolation of approximately 91% of the total UA content through a single extraction, whereas a single acetone extraction yielded only 78.4% of UA. Full article
(This article belongs to the Special Issue New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics)
►▼ Show Figures

Figure 1

13 pages, 3314 KiB  
Article
Cinnamomum japonicum Siebold Branch Extracts Attenuate NO and ROS Production via the Inhibition of p38 and JNK Phosphorylation
by Jae Min Kim, Moon-Hee Choi and Ji Hye Yang
Molecules 2023, 28(4), 1974; https://doi.org/10.3390/molecules28041974 - 19 Feb 2023
Cited by 1 | Viewed by 1397
Abstract
Cinnamomum japonicum (CJ) is widely distributed in Asian countries like Korea, China, and Japan. Modern pharmacological studies have demonstrated that it exhibits various biological activities, including antioxidant and anti-inflammatory effects. However, most studies have confirmed the efficacy of its water extract but not [...] Read more.
Cinnamomum japonicum (CJ) is widely distributed in Asian countries like Korea, China, and Japan. Modern pharmacological studies have demonstrated that it exhibits various biological activities, including antioxidant and anti-inflammatory effects. However, most studies have confirmed the efficacy of its water extract but not that of its other extracts. Therefore, in this study, Cinnamomum japonicum Siebold branches (CJB: 70% EtOH extract) were separated using hexane, chloroform, ethyl acetate (CJB3), butanol, and water. Then, their antioxidative activities and phenolic contents were measured. Results revealed that the antioxidant activities and phenolic contents of CJB3 were higher than those of the other extracts. Further, the inhibitory and anti-inflammatory effect of CJB3 on lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) production and LPS-activated macrophages, respectively, was determined. CJB3 suppressed oxidative stress in LPS-activated cells and dose-dependently decreased LPS-stimulated ROS production. CJB3 reduced oxidative stress and reversed the glutathione decrease in LPS-activated RAW264.7 cells. The inhibitory and reducing effect of CJB3 on LPS-induced nitric oxide (NO) production and inducible NO synthase protein and messenger RNA levels, respectively, was investigated. CJB3 inhibited LPS-induced cytokine production and p38 and c-Jun N-terminal kinase (JNK) phosphorylation but not extracellular signal-regulated kinase phosphorylation. Overall, the study results suggest that CJB3 may exert its anti-inflammatory effects via the suppression of p38, JNK, and c-Jun activation. Full article
(This article belongs to the Special Issue New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics)
►▼ Show Figures

Figure 1

19 pages, 3765 KiB  
Article
Isolation of the Main Biologically Active Substances and Phytochemical Analysis of Ginkgo biloba Callus Culture Extracts
by Violeta Le, Andrey Sukhikh, Timothy Larichev, Svetlana Ivanova, Alexander Prosekov and Anastasia Dmitrieva
Molecules 2023, 28(4), 1560; https://doi.org/10.3390/molecules28041560 - 06 Feb 2023
Cited by 4 | Viewed by 1760
Abstract
The work reveals the results of studying the content of biologically active substances in samples of extracts of Ginkgo biloba callus cultures. Callus cultures grown in vitro on liquid nutrient media were the objects of the study. Considering various factors affecting the yield [...] Read more.
The work reveals the results of studying the content of biologically active substances in samples of extracts of Ginkgo biloba callus cultures. Callus cultures grown in vitro on liquid nutrient media were the objects of the study. Considering various factors affecting the yield of the target components during extraction, the volume fraction of the organic modifier in the extracting mixture, the temperature factor, and the exposure time were identified as the main ones. The maximum yield of extractive substances (target biologically active substances with a degree of extraction of at least 50%) from the samples of callus culture extracts was detected at a ratio of extragent of 70% ethanol, a temperature of 50 °C, and exposure time of 6 h. Flavonoids, such as luteolin, quercetin, isoramentin, kaempferol, and amentoflavone, were isolated in the extract samples. As a result of column chromatography, fractions of individual biologically active substances (bilobalide, ginkgolide A, B, and C) were determined. The proposed schemes are focused on preserving the nativity while ensuring maximum purification from associated (ballast) components. Sorbents (Sephadex LH-20, poly-amide, silica gel) were used in successive stages of chromatography with rechromatography. The degree of purity of individually isolated substances was at least 95%. Full article
(This article belongs to the Special Issue New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics)
►▼ Show Figures

Figure 1

18 pages, 4604 KiB  
Article
Application of Natural Deep Eutectic Solvents for Extraction of Bioactive Components from Rhodiola rosea (L.)
by Nikita Tsvetov, Oksana Paukshta, Nadezhda Fokina, Natalia Volodina and Artemiy Samarov
Molecules 2023, 28(2), 912; https://doi.org/10.3390/molecules28020912 - 16 Jan 2023
Cited by 6 | Viewed by 2038
Abstract
Rhodiola rosea (L.) is a valuable source of nutrients. Nutrients have adaptogenic, immunostimulating, nootropic, anti-inflammatory and anti-cancer properties. Natural deep eutectic solvents (NADES) consisting of choline chloride and malonic, malic, tartaric or citric acids have been first used to extract biologically active substances [...] Read more.
Rhodiola rosea (L.) is a valuable source of nutrients. Nutrients have adaptogenic, immunostimulating, nootropic, anti-inflammatory and anti-cancer properties. Natural deep eutectic solvents (NADES) consisting of choline chloride and malonic, malic, tartaric or citric acids have been first used to extract biologically active substances from R. rosea. The total content of polyphenols has been determined by the Folin–Ciocalteu method for all extracts. Antioxidant activity has been determined by the phosphomolybdate method, and antiradical activity has been determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. Rosavin concentration has been determined by high-performance liquid chromatography (HPLC). Extraction kinetics has been evaluated regarding the effectiveness of NADES with each other and with reference solvents (water and 50% ethanol) has been made. Extraction conditions have been optimized according to the Box–Behnken design of the experiment. The optimal parameters of the extraction process have been established. The antibacterial activity of NADES-based extracts against bacterial cultures of Micrococcus luteus, Pseudomonas fluorescens, and Bacillus subtilis has been studied. Full article
(This article belongs to the Special Issue New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics)
►▼ Show Figures

Figure 1

12 pages, 2529 KiB  
Article
Ultrasound-Assisted Water Extraction of Gentiopicroside, Isogentisin, and Polyphenols from Willow Gentian “Dust” Supported by Hydroxypropyl-β-Cyclodextrin as Cage Molecules
by Miloš S. Jovanović, Nemanja Krgović, Katarina Šavikin and Jelena Živković
Molecules 2022, 27(21), 7606; https://doi.org/10.3390/molecules27217606 - 06 Nov 2022
Cited by 4 | Viewed by 1311
Abstract
The residue after sieving (“dust”) from the willow gentian underground parts is an unexploited herbal tea by-product, although it contains valuable bioactive compounds. Cyclodextrins as efficient green co-solvents, cage molecules, and multifunctional excipients could improve the extraction and contribute to the added value [...] Read more.
The residue after sieving (“dust”) from the willow gentian underground parts is an unexploited herbal tea by-product, although it contains valuable bioactive compounds. Cyclodextrins as efficient green co-solvents, cage molecules, and multifunctional excipients could improve the extraction and contribute to the added value of the resulting extracts. The objective of this study was to determine the optimal conditions for the extraction of gentiopicroside, isogentisin, and total phenolics (TPC) from willow gentian “dust” using ultrasound-assisted water extraction coupled with hydroxypropyl-β-cyclodextrin (HPβCD). The influence of extraction temperature (X1: 20–80 °C), time (X2: 20–50 min), and HPβCD concentration (X3: 2–4% w/v) was analyzed employing the response surface methodology (RSM). The optimal extraction conditions for simultaneously maximizing the extraction yield of all monitored responses were X1: 74.89 °C, X2: 32.57 min, and X3: 3.01% w/v. The experimentally obtained response values under these conditions (46.96 mg/g DW for gentiopicroside, 0.51 mg/g DW for isogentisin, and 12.99 mg GAE/g DW for TPC) were in close agreement with those predicted, thus confirming the suitability and good predictive accuracy of the developed RSM models. Overall, the developed extraction system could be an applicable alternative strategy to improve the extraction of bioactive compounds from the underutilized “dust” of willow gentian underground parts. Full article
(This article belongs to the Special Issue New Approaches in the Extraction of Biomolecules from Plants–Molecular and Application Basics)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-5
Back to TopTop