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Molecules
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Applications of Chromatography in Biological Sample Analysis
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Applications of Chromatography in Biological Sample Analysis

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (31 December 2023) | Viewed by 1721

Special Issue Editor

Dr. Sanka N. Atapattu

E-Mail Website
Guest Editor
CanAm Bioresearch Inc., Winnipeg, MB, Canada
Interests: chromatography; retention properties; stationary phase selectivity; derivatization methods; sample preparation methods

Special Issue Information

Dear Colleagues,

Chromatography is the default analytical tool for most analyses of biological samples. Over the years, chromatography-based analytical methods have been a subject of growing interest for the analysis of various biological matrices. To meet regulatory and quality-control standards, analytical chemists around the world are pressed to develop rapid, reliable, and sensitive methods. This Special Issue will cover both original research reports as well as and critical reviews dealing with chromatography-based analytical methods for the analysis of various biological matrices, especially focusing on current trends, emerging technologies, and the future prospects of chromatography-based methods.

Dr. Sanka N. Atapattu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • chromatography
  • biological sample analysis
  • sample preparation
  • analyte detection

Published Papers (1 paper)

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Research

15 pages, 1478 KiB  
Article
Simple HPLC-UV Method for Therapeutic Drug Monitoring of 12 Antiepileptic Drugs and Their Main Metabolites in Human Plasma
by Daniela Milosheska and Robert Roškar
Molecules 2023, 28(23), 7830; https://doi.org/10.3390/molecules28237830 - 28 Nov 2023
Cited by 1 | Viewed by 1271
Abstract
The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation [...] Read more.
The objective of the present report was to develop and validate a simple, selective, and reproducible high-performance liquid chromatography method with UV detection suitable for routine therapeutic drug monitoring of the most commonly used antiepileptic drugs and some of their metabolites. Simple precipitation of plasma proteins with acetonitrile was used for sample preparation. 10,11-dihydrocarbamazepine was used as an internal standard. Chromatographic separation of the analytes was achieved by gradient elution on a Phenyl–Hexyl column at 40 °C, using methanol and potassium phosphate buffer (25 mM; pH 5.1) as a mobile phase. The method was validated according to the FDA guidelines for bioanalytical method validation. It showed to be selective, accurate, precise, and linear over the concentration ranges of 1–50 mg/L for phenobarbital, phenytoin, levetiracetam, rufinamide, zonisamide, and lacosamide; 0.5–50 mg/L for lamotrigine, primidone, carbamazepine and 10-monohydroxycarbazepine; 0.2–10 mg/L for carbamazepine metabolites: 10,11-trans-dihydroxy-10,11-dihydrocarbamazepine and carbamazepine-10,11-epoxide; 0.1–10 mg/L for oxcarbazepine; 2–100 mg/L for felbamate and 3–150 mg/L for ethosuximide. The suitability of the validated method for routine therapeutic drug monitoring was confirmed by quantification of the analytes in plasma samples from patients with epilepsy on combination antiepileptic therapy. Full article
(This article belongs to the Special Issue Applications of Chromatography in Biological Sample Analysis)
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