Applications of HPLC Methods in Natural Products Chemistry
A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".
Deadline for manuscript submissions: closed (31 October 2023) | Viewed by 2401
Special Issue Editors
2. Hortus Novus srl, Via Campo Sportivo 2, 67050 Canistro, Italy
Interests: discriminant analysis; geographical classification; HPLC-DAD; LDA; DoE; saffron; phytochemistry
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
High-performance liquid chromatography (HPLC) is a highly effective and flexible chromatographic technique used to separate natural products (NPs) present in complex matrices like crude extracts. It enables selective detection and quantification or general profiling of NPs. HPLC is a widely accepted and adaptable technique that can analyze a broad range of NPs with minimal sample preparation. Selecting the appropriate detection method is crucial while using HPLC as NPs are diverse and cannot be detected efficiently using a single technique. The choice of detection method depends on the specific properties of the NP being analyzed, such as its polarity, size, and functional groups. Some commonly used detection methods in HPLC include UV-Vis spectrophotometry, fluorescence spectroscopy, mass spectrometry (MS), and nuclear magnetic resonance (NMR) spectroscopy. UV-Vis spectrophotometry is the most widely used detection method in HPLC due to its sensitivity and broad applicability. It can detect NPs with chromophores that absorb light in the UV-Vis range. Fluorescence spectroscopy is another detection method that can be used for NPs with fluorescent properties. MS and NMR spectroscopy are more advanced and powerful techniques that are used for structural elucidation of NPs. MS provides molecular weight and fragmentation patterns of the NP, while NMR spectroscopy provides information about the NP's structure, functional groups, and stereochemistry. In conclusion, HPLC is a versatile and powerful technique for the separation and detection of NPs. The choice of detection method is crucial for efficient and accurate analysis of NPs, and it depends on the specific properties of the NP being analyzed. The broad applicability and adaptability of HPLC make it an indispensable tool in natural product chemistry research. In natural product chemistry, chemometrics plays an important role in data analysis and interpretation, enabling the identification of patterns and trends in the data, and the development of predictive models. This is particularly important in the analysis of complex natural product mixtures, where the identification and quantification of individual compounds can be challenging.
Dr. Maria Anna Maggi
Dr. Fabrizio Ruggieri
Guest Editors
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Keywords
- HPLC
- natural products
- chemical analysis
- detection methods
- chemometrics
