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Versatility of G-quadruplexes

A topical collection in Molecules (ISSN 1420-3049). This collection belongs to the section "Macromolecular Chemistry".

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Editors

Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Poznan, Poland
Interests: G-quadruplex; DNAzyme; DNA probes; Bioassay development; bioanalysis
Special Issues, Collections and Topics in MDPI journals
Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Poznan, Poland
Interests: G-quadruplex; i-motif; DNA probes; DNA-templated silver nanoclusters Bioassay development; bioanalysis
Special Issues, Collections and Topics in MDPI journals
Laboratory for Biomolecular and Medical Technologies, Krasnoyarsk State Medical University Named after Prof. V.F. Voyno-Yasenetsky, 660022 Krasnoyarsk, Russia
Interests: aptamer; intramer; cancer; biological activity, cell culture; in vivo experiments; tumor cells
Department of Bioanalytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Poznan, Poland
Interests: G-quadruplex; DNAzyme; DNA probes; bioassay development; bioanalysis

Topical Collection Information

Dear Colleagues,

G-quadruplex, secondary tetraplex structures formed by DNA and RNA rich in guanosine residues, has gained interest due to its biological function as well as biomedical and bioanalytical applications. Sequences able to form G-quadruplexes were found in telomeres, promotors and other regulatory domains. The formation of G-quadruplexes was also visualized in vivo. The ongoing research in this field focuses on the understanding of the G-quadruplex function in genome as well as other nucleic acids in the cell (e.g., microRNA). On the other hand, G-quadruplexes are used in the development of many bioassays thanks to their outstanding abilities, such as structure switching (e.g., under the influence of cations), the formation of many aptamers, and the formation of DNAzyme with peroxidase activity. This versatility makes G-quadruplex an eligible target for biomedical and bioanalytical research.

Prof. Dr. Bernard Juskowiak
Dr. Anna Renata Dembska
Dr. Anna S. Kichkailo
Dr. Joanna Kosman
Collection Editors

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Keywords

  • G-quadruplex structure
  • biology of G-quadruplexes
  • G-quadruplex probes
  • G-quadruplex aptamers
  • DNAzymes
  • G-quadruplexes in medicine
  • G-quadruplex in assay development

Published Papers (3 papers)

2023

Jump to: 2022

14 pages, 2865 KiB  
Article
G-Quadruplexes Formation by the C9orf72 Nucleotide Repeat Expansion d(GGGGCC)n and Conformation Regulation by Fangchinoline
by Yun Zhang, Junliu Huang, Kainan Yu and Xiaojie Cui
Molecules 2023, 28(12), 4671; https://doi.org/10.3390/molecules28124671 - 09 Jun 2023
Viewed by 1104
Abstract
The G-quadruplex (GQ)-forming hexanucleotide repeat expansion (HRE) in the C9orf72 (C9) gene has been found to be the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (collectively, C9ALS/FTD), implying the great significance of modulating C9-HRE GQ structures in C9ALS/FTD [...] Read more.
The G-quadruplex (GQ)-forming hexanucleotide repeat expansion (HRE) in the C9orf72 (C9) gene has been found to be the most common cause of amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD) (collectively, C9ALS/FTD), implying the great significance of modulating C9-HRE GQ structures in C9ALS/FTD therapeutic treatment strategies. In this study, we investigated the GQ structures formed by varied lengths of C9-HRE DNA sequences d(GGGGCC)4 (C9-24mer) and d(GGGGCC)8 (C9-48mer), and found that the C9-24mer forms anti-parallel GQ (AP-GQ) in the presence of potassium ions, while the long C9-48mer bearing eight guanine tracts forms unstacked tandem GQ consisting of two C9-24mer unimolecular AP-GQs. Moreover, the natural small molecule Fangchinoline was screened out in order to be able to stabilize and alter the C9-HRE DNA to parallel GQ topology. Further study of the interaction of Fangchinoline with the C9-HRE RNA GQ unit r(GGGGCC)4 (C9-RNA) revealed that it can also recognize and improve the thermal stability of C9-HRE RNA GQ. Finally, use of AutoDock simulation results indicated that Fangchinoline binds to the groove regions of the parallel C9-HRE GQs. These findings pave the way for further studies of GQ structures formed by pathologically related long C9-HRE sequences, and also provide a natural small-molecule ligand that modulates the structure and stability of C9-HRE GQ, both in DNA and RNA levels. Altogether, this work may contribute to therapeutic approaches of C9ALS/FTD which take the upstream C9-HRE DNA region, as well as the toxic C9-HRE RNA, as targets. Full article
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Graphical abstract

2022

Jump to: 2023

16 pages, 1858 KiB  
Review
Aptamers Regulating the Hemostasis System
by Anatoly A. Vaganov, Tatiana E. Taranushenko, Natalia A. Luzan, Irina A. Shchugoreva, Olga S. Kolovskaya, Polina V. Artyushenko, Tatiana N. Zamay and Anna S. Kichkailo
Molecules 2022, 27(23), 8593; https://doi.org/10.3390/molecules27238593 - 06 Dec 2022
Cited by 1 | Viewed by 1907
Abstract
The hemostasis system is a complex structure that includes the fibrinolysis system, and Yes this is correct coagulation and anticoagulation parts. Due to the multicomponent nature, it becomes relevant to study the key changes in the functioning of signaling pathways, and develop new [...] Read more.
The hemostasis system is a complex structure that includes the fibrinolysis system, and Yes this is correct coagulation and anticoagulation parts. Due to the multicomponent nature, it becomes relevant to study the key changes in the functioning of signaling pathways, and develop new diagnostic methods and modern drugs with high selectivity. One of the ways to solve this problem is the development of molecular recognition elements capable of blocking one of the hemostasis systems and/or activating another. Aptamers can serve as ligands for targeting specific clinical needs, promising anticoagulants with minor side effects and significant biological activity. Aptamers with several clotting factors and platelet proteins are used for the treatment of thrombosis. This review is focused on the aptamers used for the correction of the hemostasis system, and their structural and functional features. G-rich nucleic acid aptamers, mostly versatile G-quadruplexes, recognize different components of the hemostasis system and are capable of correcting the functioning. Full article
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Graphical abstract

13 pages, 4474 KiB  
Article
Naphthalene Diimides Carrying Two β-Cyclodextrins Prefer Telomere RNA G-Quadruplex Recognition
by Tingting Zou, Yuka Sato, Shuma Kaneyoshi, Kota Mano, Rui Yasukawa, Yoshifumi Nakano, Satoshi Fujii, Shinobu Sato and Shigeori Takenaka
Molecules 2022, 27(13), 4053; https://doi.org/10.3390/molecules27134053 - 23 Jun 2022
Viewed by 1703
Abstract
Newly synthesized naphthalene diimide carrying two β-cyclodextrins (NDI-β-CyDs) showed improved specificity for the parallel G-quadruplex structure alongside the hybrid G-quadruplex structure. Specifically, the highest binding affinity of NDI-β-CyDs for the telomere RNA G-quadruplex was observed. The binding simulation indicated that β-cyclodextrins might be [...] Read more.
Newly synthesized naphthalene diimide carrying two β-cyclodextrins (NDI-β-CyDs) showed improved specificity for the parallel G-quadruplex structure alongside the hybrid G-quadruplex structure. Specifically, the highest binding affinity of NDI-β-CyDs for the telomere RNA G-quadruplex was observed. The binding simulation indicated that β-cyclodextrins might be available for loop nucleobase inclusion under its complex. Full article
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Figure 1

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