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Theme Issue in Honor of Professor Ana Maria Oliveira Brett: Bioelectrochemical Sensing and DNA Electrochemical Biosensors

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 November 2021) | Viewed by 21158

Special Issue Editor

Special Issue Information

Dear Colleagues,

This Special Issue will honor Prof. Ana Maria Oliveira Brett for her outstanding contributions in the areas of bioelectrochemistry and bioelectroanalysis. Prof. Oliveira Brett is a chemist; she obtained her BSc Chemistry at the University of Coimbra in 1973, and then her PhD in Electrochemistry at the University of London, Imperial College, in 1980. She is Professor in the Department of Chemistry of University of Coimbra. 

She is also a member of the Centre for Mechanical Engineering, Materials and Processes (CEMMPRE), Research Unit 285 of Fundacao para a Ciencia e Tecnologia (FCT), and director of the Laboratory of Electroanalysis and Corrosion (IPN-LEC), Instituto Pedro Nunes in Coimbra.

She was the President of the International Bioelectrochemical Society (BES) in 2007–2015, and Past-President in 2016–2017. She was Associate Editor of the Journal Bioelectrochemistry from 2000 to 2016.

She is a Member of the Editorial Board of the international journals Bioelectrochemistry, Analytical Letters, Current Opinion in Electrochemistry, International Journal of Clinical Neurosciences and Mental Health, and Journal of Research in Pharmacy.

She is a member of various Scientific Societies and has been an active member collaborating during different periods as a Division Officer of Analytical Electrochemistry and of Bioelectrochemistry, in the International Society of Electrochemistry (ISE).

She is an IUPAC Fellow, Fellow of the Royal Society of Chemistry (FRSC), and Chartered Chemist (CChem) (UK).

Her research activity is centered on fundamental aspects in the areas of bioelectrochemistry, the study of electron transfer reactions of compounds of biological interest, bioelectroanalysis, and the development of enzymes and DNA biosensors. Current efforts include studies of the morphology of DNA adsorbed at solid charged interfaces, electrochemical detection of the mechanisms of DNA–drug interactions, and evaluation of oxidative damage caused to DNA by health hazardous compounds. Related research is concerned with the study of electron transfer reactions of antioxidants and understanding the free-radical-induced damaging aspects of the chemistry of disease processes.

Research activity and collaboration with national and international groups is documented by Prof. Oliveira Brett’s projects and publications: she has published more than 230 papers, is the co-author of 2 undergraduate/graduate textbooks, both published by Oxford University Press (one also translated into Portuguese), 16 chapters in multi-author books, has attended and presented research work in research conferences, and has been invited to present more than 90 lectures, including plenary lectures in international conferences and invited seminars.

All her remarkable scientific contributions cannot be contained in the limited space of a journal, but the least I can do, as friend and colleague who has had the opportunity to know and appreciate her, is to produce a Special Issue dedicated to her.

It is my great pleasure to invite you to submit a manuscript to this Special Issue; regular articles, communications, as well as reviews concerning the general theme of “Bioelectrochemical Sensing and DNA Electrochemical Biosensors” are all welcome.

Prof. Dr. Antonella Curulli
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bioelectrochemistry
  • bioelectrochemical sensing
  • electrochemical biosensors
  • DNA biosensors

Published Papers (4 papers)

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Research

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17 pages, 2263 KiB  
Article
Design and Evaluation of a Lactate Microbiosensor: Toward Multianalyte Monitoring of Neurometabolic Markers In Vivo in the Brain
by Eliana Fernandes, Ana Ledo and Rui M. Barbosa
Molecules 2022, 27(2), 514; https://doi.org/10.3390/molecules27020514 - 14 Jan 2022
Cited by 6 | Viewed by 2226
Abstract
Direct in vivo measurements of neurometabolic markers in the brain with high spatio-temporal resolution, sensitivity, and selectivity is highly important to understand neurometabolism. Electrochemical biosensors based on microelectrodes are very attractive analytical tools for continuous monitoring of neurometabolic markers, such as lactate and [...] Read more.
Direct in vivo measurements of neurometabolic markers in the brain with high spatio-temporal resolution, sensitivity, and selectivity is highly important to understand neurometabolism. Electrochemical biosensors based on microelectrodes are very attractive analytical tools for continuous monitoring of neurometabolic markers, such as lactate and glucose in the brain extracellular space at resting and following neuronal activation. Here, we assess the merits of a platinized carbon fiber microelectrode (CFM/Pt) as a sensing platform for developing enzyme oxidase-based microbiosensors to measure extracellular lactate in the brain. Lactate oxidase was immobilized on the CFM/Pt surface by crosslinking with glutaraldehyde. The CFM/Pt-based lactate microbiosensor exhibited high sensitivity and selectivity, good operational stability, and low dependence on oxygen, temperature, and pH. An array consisting of a glucose and lactate microbiosensors, including a null sensor, was used for concurrent measurement of both neurometabolic substrates in vivo in the anesthetized rat brain. Rapid changes of lactate and glucose were observed in the cortex and hippocampus in response to local glucose and lactate application and upon insulin-induced fluctuations of systemic glucose. Overall, these results indicate that microbiosensors are a valuable tool to investigate neurometabolism and to better understand the role of major neurometabolic markers, such as lactate and glucose. Full article
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11 pages, 1536 KiB  
Article
DNA-Based Electrodes and Computational Approaches on the Intercalation Study of Antitumoral Drugs
by Edson Silvio Batista Rodrigues, Isaac Yves Lopes de Macêdo, Giovanna Nascimento de Mello e Silva, Arthur de Carvalho e Silva, Henric Pietro Vicente Gil, Bruno Junior Neves and Eric de Souza Gil
Molecules 2021, 26(24), 7623; https://doi.org/10.3390/molecules26247623 - 16 Dec 2021
Cited by 2 | Viewed by 2214
Abstract
The binding between anticancer drugs and double-stranded DNA (dsDNA) is a key issue to understand their mechanism of action, and many chemical methods have been explored on this task. Molecular docking techniques successfully predict the affinity of small molecules into the DNA binding [...] Read more.
The binding between anticancer drugs and double-stranded DNA (dsDNA) is a key issue to understand their mechanism of action, and many chemical methods have been explored on this task. Molecular docking techniques successfully predict the affinity of small molecules into the DNA binding sites. In turn, various DNA-targeted drugs are electroactive; in this regard, their electrochemical behavior may change according to the nature and strength of interaction with DNA. A carbon paste electrode (CPE) modified with calf thymus ds-DNA (CPDE) and computational methods were used to evaluate the drug–DNA intercalation of doxorubicin (DOX), daunorubicin (DAU), idarubicin (IDA), dacarbazine (DAR), mitoxantrone (MIT), and methotrexate (MTX), aiming to evaluate eventual correlations. CPE and CPDE were immersed in pH 7 0.1 mM solutions of each drug with different incubation times. As expected, the CPDE response for all DNA-targeted drugs was higher than that of CPE, evidencing the drug–DNA interaction. A peak current increase of up to 10-fold was observed; the lowest increase was seen for MTX, and the highest increase for MIT. Although this increase in the sensitivity is certainly tied to preconcentration effects of DNA, the data did not agree entirely with docking studies, evidencing the participation of other factors, such as viscosity, interfacial electrostatic interactions, and coefficient of diffusion. Full article
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Review

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18 pages, 5538 KiB  
Review
8-oxoguanine and 8-oxodeoxyguanosine Biomarkers of Oxidative DNA Damage: A Review on HPLC–ECD Determination
by Ana-Maria Chiorcea-Paquim
Molecules 2022, 27(5), 1620; https://doi.org/10.3390/molecules27051620 - 01 Mar 2022
Cited by 44 | Viewed by 5188
Abstract
Reactive oxygen species (ROS) are continuously produced in living cells due to metabolic and biochemical reactions and due to exposure to physical, chemical and biological agents. Excessive ROS cause oxidative stress and lead to oxidative DNA damage. Within ROS-mediated DNA lesions, 8-oxoguanine (8-oxoG) [...] Read more.
Reactive oxygen species (ROS) are continuously produced in living cells due to metabolic and biochemical reactions and due to exposure to physical, chemical and biological agents. Excessive ROS cause oxidative stress and lead to oxidative DNA damage. Within ROS-mediated DNA lesions, 8-oxoguanine (8-oxoG) and its nucleotide 8-oxo-2′-deoxyguanosine (8-oxodG)—the guanine and deoxyguanosine oxidation products, respectively, are regarded as the most significant biomarkers for oxidative DNA damage. The quantification of 8-oxoG and 8-oxodG in urine, blood, tissue and saliva is essential, being employed to determine the overall effects of oxidative stress and to assess the risk, diagnose, and evaluate the treatment of autoimmune, inflammatory, neurodegenerative and cardiovascular diseases, diabetes, cancer and other age-related diseases. High-performance liquid chromatography with electrochemical detection (HPLC–ECD) is largely employed for 8-oxoG and 8-oxodG determination in biological samples due to its high selectivity and sensitivity, down to the femtomolar range. This review seeks to provide an exhaustive analysis of the most recent reports on the HPLC–ECD determination of 8-oxoG and 8-oxodG in cellular DNA and body fluids, which is relevant for health research. Full article
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17 pages, 3664 KiB  
Review
Electrochemical Impedance Spectroscopy in the Characterisation and Application of Modified Electrodes for Electrochemical Sensors and Biosensors
by Christopher M. A. Brett
Molecules 2022, 27(5), 1497; https://doi.org/10.3390/molecules27051497 - 23 Feb 2022
Cited by 63 | Viewed by 9930
Abstract
Electrochemical impedance spectroscopy is finding increasing use in electrochemical sensors and biosensors, both in their characterisation, including during successive phases of sensor construction, and in application as a quantitative determination technique. Much of the published work continues to make little use of all [...] Read more.
Electrochemical impedance spectroscopy is finding increasing use in electrochemical sensors and biosensors, both in their characterisation, including during successive phases of sensor construction, and in application as a quantitative determination technique. Much of the published work continues to make little use of all the information that can be furnished by full physical modelling and analysis of the impedance spectra, and thus does not throw more than a superficial light on the processes occurring. Analysis is often restricted to estimating values of charge transfer resistances without interpretation and ignoring other electrical equivalent circuit components. In this article, the important basics of electrochemical impedance for electrochemical sensors and biosensors are presented, focussing on the necessary electrical circuit elements. This is followed by examples of its use in characterisation and in electroanalytical applications, at the same time demonstrating how fuller use can be made of the information obtained from complete modelling and analysis of the data in the spectra, the values of the circuit components and their physical meaning. The future outlook for electrochemical impedance in the sensing field is discussed. Full article
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