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New Approaches for the Isolation of Natural Products

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Organic Chemistry".

Deadline for manuscript submissions: closed (30 August 2020) | Viewed by 6916

Special Issue Editor

1. School of Chemical and Physical Sciences, and Center For Biodiscovery, Victoria University of Wellington, Wellington 6140, New Zealand
2. Maurice Wilkins Centre for Molecular Biodiscovery, Auckland, New Zealand
Interests: marine natural products; chromatography; bioactives; spectroscopy; NMR; MS
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Given the aeons relating to their evolution, natural products continue to be a major and valuable resource for the discovery of new biochemical probes and medicinal lead compounds. Sources of natural products vary widely, from terrestrial plants and microorganisms to marine invertebrate animals and plants, while compounds come from a number of biosynthetic pathways including terpenes, alkaloids, polyketides, peptides, and polyphenolics. Key to the discovery of natural products is the development of new and innovative techniques and methodologies that optimize and streamline both the isolation and structural elucidation process. The aim of this Special Issue of Molecules is to highlight recent developments in the detection, isolation, structure elucidation, and biochemical evaluation of bioactive natural products. Communications, full papers, and review articles are all welcome for submission.

Dr. Rob Keyzers
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Screening methodology
  • Chromatography
  • Bioassay guided
  • Spectroscopy

Published Papers (2 papers)

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Research

10 pages, 2377 KiB  
Article
Anti-Inflammatory and Cytotoxic Potential of New Phenanthrenoids from Luzula sylvatica
by Maël Gainche, Isabelle Ripoche, François Senejoux, Juliette Cholet, Clémence Ogeron, Caroline Decombat, Ombeline Danton, Laetitia Delort, Marjolaine Vareille-Delarbre, Alexandre Berry, Marion Vermerie, Didier Fraisse, Catherine Felgines, Edwige Ranouille, Jean-Yves Berthon, Julien Priam, Etienne Saunier, Albert Tourrette, Yves Troin, Florence Caldefie-Chezet and Pierre Chalardadd Show full author list remove Hide full author list
Molecules 2020, 25(10), 2372; https://doi.org/10.3390/molecules25102372 - 20 May 2020
Cited by 3 | Viewed by 2511
Abstract
Phenanthrenoids have been widely described, in the Juncaceae family, for their biological properties such as antitumor, anxiolytic, anti-microbial, spasmolytic, and anti-inflammatory activities. The Juncaceae family is known to contain a large variety of phenanthrenoids possessing especially anti-inflammatory and cytotoxic properties. Luzula sylvatica, [...] Read more.
Phenanthrenoids have been widely described, in the Juncaceae family, for their biological properties such as antitumor, anxiolytic, anti-microbial, spasmolytic, and anti-inflammatory activities. The Juncaceae family is known to contain a large variety of phenanthrenoids possessing especially anti-inflammatory and cytotoxic properties. Luzula sylvatica, a Juncaceae species, is widely present in the Auvergne region of France, but has never been studied neither for its phytochemical profile nor for its biological properties. We investigated the phytochemical profile and evaluated the potential anti-inflammatory activities of L. sylvatica aerial parts extracts. A bioassay-guided fractionation was carried out to identify the most active fractions. Nine compounds were isolated, one coumarin 1 and eight phenanthrene derivatives (29), including four new compounds (4, 5, 8 and 9), from n-hexane and CH2Cl2, fractions. Their structures were established by HRESIMS, 1D and 2D NMR experiments. The biological properties, especially the anti-inflammatory/antioxidant activities (ROS production) and antiproliferative activity on THP-1, a monocytic leukemia cell line, of each compound, were evaluated. Three phenanthrene derivatives 4, 6, and 7 showed very promising antiproliferative activities. Full article
(This article belongs to the Special Issue New Approaches for the Isolation of Natural Products)
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16 pages, 2350 KiB  
Article
Antileukemic Cell Proliferation of Active Compounds from Kaffir Lime (Citrus hystrix) Leaves
by Songyot Anuchapreeda, Fah Chueahongthong, Natsima Viriyaadhammaa, Pawaret Panyajai, Riki Anzawa, Singkome Tima, Chadarat Ampasavate, Aroonchai Saiai, Methee Rungrojsakul, Toyonobu Usuki and Siriporn Okonogi
Molecules 2020, 25(6), 1300; https://doi.org/10.3390/molecules25061300 - 12 Mar 2020
Cited by 24 | Viewed by 3977
Abstract
Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated [...] Read more.
Kaffir lime (Citrus hystrix) is a plant member of family Rutaceae, and its leaves are commonly used in folk medicine. The present study explores antileukemic effects of the extracts and purified active compounds from the leaves. The antileukemic activity was investigated via inhibition of Wilms’ tumor 1 (WT1), which is a protein that involves in leukemic cell proliferation. In addition, the compounds were investigated for their effects on WT1 gene expression using real time RT-PCR and Western blotting. Cell cycle arrest and total cell number were investigated using flow cytometry and trypan blue exclusion method, respectively. The results demonstrated that the hexane fractionated extract had the greatest inhibitory effect on WT1 gene expression of many leukemic cell lines and significantly decreased WT1 protein levels of K562 cells (representative of the leukemic cells), in a dose- and time-dependent manner. Subfraction No. 9 (F9) after partial purification of hexane fractioned extract showed the highest suppression on WT1 protein and suppressed cell cycle at G2/M. The organic compounds were isolated from F9 and identified as phytol and lupeol. The bioassays confirmed antiproliferative activities of natural products phytol and lupeol. The results demonstrated anticancer activity of the isolated phytol and lupeol to decrease leukemic cell proliferation. Full article
(This article belongs to the Special Issue New Approaches for the Isolation of Natural Products)
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