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New Technology and Method to Rapidly Detect Nucleic Acid

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 1941

Special Issue Editors

School of Basic Medical Sciences, Anhui Medical University, Hefei 230032, China
Interests: ion channel; Ca2+ signaling; mitochondria; mitophagy; endothelial cell; vascular smooth muscle; ischemia/reperfusion injury; neurodegenerative diseases

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Guest Editor
School of Pharmaceutical Science and Yunnan Key Laboratory of Pharmacology for Natural Products, Kunming Medical University, Kunming 650500, China
Interests: cardiovascular diseases; viruses; hypertension; cardiac hypertrophy

Special Issue Information

Dear Colleagues,

Nucleic acid mainly includes DNA, RNA and Non-coding RNA. Detection of pathogen nucleic acid is an important means of clinical diagnosis of pathogen infection. As COVID-19 spreading around the world, rapid and accurate nucleic acid testing can help epidemiologists quickly find the source of infection and effectively control the spread of the epidemic. However, the most commonly used nucleic acid detection method in clinical is still the traditional qPCR. Although qPCR technology has many advantages, it also has problems such as reliance on professionals and equipment, needs for multiple steps of nucleic acid extraction, amplification and result analysis, and contamination. In addition to qPCR, the emerging isothermal amplification, DNA nanotechnology, and CRISPR/Cas techniques mature gradually in very recent years. Therefore, it is very important to develop novel nucleic acid detection methods with rapid reaction, simple methods, high sensitivity, specificity and selectivity, portable, and low cost.

This Special Issue provides a broad survey to release your most recent work on new strategies and technologies on rapid detection of nucleic acid in various areas. Original research articles or reviews aiming at the development and applications of rapid detection of nucleic acid in different fields are welcome.

Dr. Bing Shen
Dr. Changbo Zheng
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • nucleic acid
  • diagnosis
  • rapid reaction
  • simple methods
  • high sensitivity
  • high specificity
  • high selectivity

Published Papers (1 paper)

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Research

13 pages, 2649 KiB  
Article
Comparison of DNA–Gold Nanoparticle Conjugation Methods: Application in Lateral Flow Nucleic Acid Biosensors
by Qiaoling Ding, Wanwei Qiu, Chunxue Sun, Hongxin Ren and Guodong Liu
Molecules 2023, 28(11), 4480; https://doi.org/10.3390/molecules28114480 - 01 Jun 2023
Cited by 3 | Viewed by 1693
Abstract
Lateral flow nucleic acid biosensors (LFNABs) have attracted extensive attention due to their rapid turnaround time, low cost, and results that are visible to the naked eye. One of the key steps to develop LFNABs is to prepare DNA–gold nanoparticle (DNA–AuNP) conjugates, which [...] Read more.
Lateral flow nucleic acid biosensors (LFNABs) have attracted extensive attention due to their rapid turnaround time, low cost, and results that are visible to the naked eye. One of the key steps to develop LFNABs is to prepare DNA–gold nanoparticle (DNA–AuNP) conjugates, which affect the sensitivity of LFNABs significantly. To date, various conjugation methods—including the salt-aging method, microwave-assisted dry heating method, freeze–thaw method, low-pH method, and butanol dehydration method—have been reported to prepare DNA–AuNP conjugates. In this study, we conducted a comparative analysis of the analytical performances of LFNABs prepared with the above five conjugation methods, and we found that the butanol dehydration method gave the lowest detection limit. After systematic optimization, the LFNAB prepared with the butanol dehydration method had a detection limit of 5 pM for single-strand DNA, which is 100 times lower than that of the salt-aging method. The as-prepared LFNAB was applied to detect miRNA-21 in human serum, with satisfactory results. The butanol dehydration method thus offers a rapid conjugation approach to prepare DNA–AuNP conjugates for LFNABs, and it can also be extended to other types of DNA biosensors and biomedical applications. Full article
(This article belongs to the Special Issue New Technology and Method to Rapidly Detect Nucleic Acid)
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