Detection of Pathogenic Microorganism

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (15 February 2024) | Viewed by 1318

Special Issue Editor


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Guest Editor
Division of Microbiology, National Center for Toxicological Research, U.S. Food and Drug Administration, Jefferson, AR 72079, USA
Interests: detection of microbial contamination; Burkholderia cepacia complex; food microbiology; evaluation of microbial contaminants; environmental microbiology; metagenomics

Special Issue Information

Dear Colleagues,

Pathogens are responsible for countless outbreaks of disease among humans, with significant impacts on public health and the economy, and with some possessing stark mortality rates. Pathogenic microorganism detection and identification is a fundamental component of the successful response to and control of epidemics and pandemics caused by bacteria and viruses. This essential component depends on the availability of robust diagnostic tools, which constitute the front-line defense in the fight against epidemics/pandemics in public health. Nevertheless, the scientific community must develop innovative diagnostics that provide sample-to-answer techniques (e.g., testing devices and technologies) as they enable first responders to readily analyse on-site data in the field, an area where molecular and biochemical diagnostics are badly needed. Scientific research is therefore crucial for a better understanding of pathogenicity, anti-microbial resistance virulence factors transfer, and drug escape mechanisms through sound studies of pathogens’ close neighbors at the biochemical and genetic levels.

The aim of this Special Issue is to publish a collection of articles relating to various strategies used to prevent, control and detect the occurrence of pathogenic microorganisms with the ultimate aims of suppressing their survival, multiplication, and entry into the human body. Manuscripts addressing the diagnostic and detection methodologies of pathogenic microorganisms are welcome in this Special Issue.

Dr. Youngbeom Ahn
Guest Editor

Manuscript Submission Information

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Keywords

  • emerging bacterial and viral infectious diseases
  • pathogenic microorganism prevention and detection methods
  • molecular and biochemical diagnostics
  • testing and technology ways
  • diagnostics validation

Published Papers (1 paper)

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Research

6 pages, 586 KiB  
Communication
Clinical Evaluation and Comparison of Two Microfluidic Antigenic Assays for Detection of SARS-CoV-2 Virus
by Paolo Bottino, Valentina Pizzo, Salvatore Castaldo, Elisabetta Scomparin, Cristina Bara, Marcella Cerrato, Sabrina Sisinni, Serena Penpa, Annalisa Roveta, Maria Gerbino, Antonio Maconi and Andrea Rocchetti
Microorganisms 2023, 11(11), 2709; https://doi.org/10.3390/microorganisms11112709 - 05 Nov 2023
Cited by 1 | Viewed by 974
Abstract
Given the ongoing pandemic, there is a need to identify SARS-CoV-2 and differentiate it from other respiratory viral infections in various critical settings. Since its introduction, rapid antigen testing is spreading worldwide, but diagnostic accuracy is extremely variable and often in disagreement with [...] Read more.
Given the ongoing pandemic, there is a need to identify SARS-CoV-2 and differentiate it from other respiratory viral infections in various critical settings. Since its introduction, rapid antigen testing is spreading worldwide, but diagnostic accuracy is extremely variable and often in disagreement with the manufacturer’s specifications. Our study compared the clinical performances of two microfluidic rapid antigen tests towards a molecular assay, starting from positive samples. A total of 151 swabs collected at the Microbiology and Virology Laboratory of A.O. “SS Antonio e Biagio e C. Arrigo” (Alessandria, Italy) for the diagnosis of SARS-CoV-2 were simultaneously tested to evaluate accuracy, specificity, and agreement with the RT-qPCR results. Both assays showed an overall agreement of 100% for negative specimens, while positive accuracy comprised between 45.10% and 54.90%. According to the manufacturer’s instructions, the greatest correlation between the antigenic and molecular assays was observed for the subset with high viral load (18/19, 94.74%), while it dramatically decreased for other subsets. Moreover, the ability to differentiate between SARS-CoV-2 and Flu provides an added value and could be addressed in an epidemic context. However, an in-house validation should be performed due to differences observed in performance declared by manufacturers and those actually obtained. Full article
(This article belongs to the Special Issue Detection of Pathogenic Microorganism)
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