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Microorganisms
Special Issues
Detection and Epidemiology of Foodborne Pathogens
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Detection and Epidemiology of Foodborne Pathogens

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Food Microbiology".

Deadline for manuscript submissions: closed (31 May 2022) | Viewed by 9652

Special Issue Editor

Dr. Pratik Banerjee

E-Mail Website
Guest Editor
Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL, USA
Interests: food safety; rapid diagnostics; molecular epidemiology; biosensors; risk assessment

Special Issue Information

Dear Colleagues,

With the advent of new tools and methodologies, there is a paradigm shift in the diagnostics and epidemiology of foodborne pathogens. In particular, advanced molecular tools, including next-generation sequencing (NGS), have improved the diagnostic resolution and epidemiologic surveillance of these pathogens. Thus, sequencing-based approaches, such as whole-genome sequencing (WGS), are gradually becoming typing methods of choice. Similarly, these approaches and advancements in associated microbial bioinformatics and computational tools have increased the application of culture-independent diagnostic tests (CIDTs) in clinical diagnostics. Therefore, it is imperative that CIDT soon be routinely used in food safety diagnostics as well. On the other hand, rapid methods utilizing techniques such as immunoassays and other microbial-associated entities (e.g., surface proteins) on micro- and nanoscale sensing devices have enabled the rapid detection of different pathogens from food commodities or food environments. In summary, the resolution of microbial food safety has improved significantly owing to the rapid techniques of detection and characterization of these pathogens, resulting in prompt and effective public health responses.

The aim of this Special Issue, “Detection and Epidemiology of Foodborne Pathogens”, is to provide a platform to disseminate new and exciting research findings in this rapidly evolving area.

As the Guest Editor of the Special Issue, I invite you to submit research articles, review articles, and short communications related to the detection and epidemiology of foodborne pathogens.

Dr. Pratik Banerjee
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Foodborne pathogens
  • Microbial molecular epidemiology
  • Next-generation sequencing (NGS)
  • Culture-independent diagnostic test (CIDT)
  • Rapid microbial diagnostics
  • Biosensors
  • Immunosensors
  • Lab-on-a-chip
  • Antimicrobial resistance
  • Microbial risk assessment

Published Papers (4 papers)

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Research

20 pages, 1778 KiB  
Article
Epidemiological Study on Salmonella Prevalence in Sow Herds Using Direct and Indirect Detection Methods
by Isabell Hollmann, Jan Berend Lingens, Volker Wilke, Christian Homann, Klaus Teich, Juhle Buch, Bussarakam Chuppava and Christian Visscher
Microorganisms 2022, 10(8), 1532; https://doi.org/10.3390/microorganisms10081532 - 28 Jul 2022
Cited by 3 | Viewed by 1656
Abstract
In piglet production, the beginning of pork production, Salmonella prevalence requires greater attention as having an impact on the subsequent production steps. The aim of this study was to investigate Salmonella prevalence in three sow herds with attached piglet rearing units. Salmonella prevalence [...] Read more.
In piglet production, the beginning of pork production, Salmonella prevalence requires greater attention as having an impact on the subsequent production steps. The aim of this study was to investigate Salmonella prevalence in three sow herds with attached piglet rearing units. Salmonella prevalence was investigated either directly by boot swabs and feces or indirectly by serum samples taken during gilt integration, the peripartal period, and piglet rearing. Boot swabs and feces were analyzed by real-time PCR and subsequent microbiology. Results indicated that high biosecurity measures in sow husbandry do not necessarily result in a low Salmonella prevalence. Furthermore, the sow herds’ Salmonella prevalence should not be used to infer the situation in the associated piglet rearing. The proportion of positive boot swabs was 10.5, 3.6, and 21.3% for sows (gilts and peripartal) with an inverse situation in piglet rearing with 50.0, 63.3, and 5.8% positive swabs for farms A, B, and C, respectively. Boot swabs are suitable as a direct sampling method to gain an overview of Salmonella prevalence in both sows and piglets. Indirect serum antibody testing can be useful, although it should be evaluated considering age-dependent levels of antibody titres. Full article
(This article belongs to the Special Issue Detection and Epidemiology of Foodborne Pathogens)
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16 pages, 2203 KiB  
Article
Simultaneous Detection of Five Foodborne Pathogens Using a Mini Automatic Nucleic Acid Extractor Combined with Recombinase Polymerase Amplification and Lateral Flow Immunoassay
by Bei Jin, Biao Ma, Jiali Li, Yi Hong and Mingzhou Zhang
Microorganisms 2022, 10(7), 1352; https://doi.org/10.3390/microorganisms10071352 - 05 Jul 2022
Cited by 14 | Viewed by 2240
Abstract
In recent years, foodborne disease outbreaks have caused huge losses to the economy and have had severe impacts on public health. The accuracy and variety of detection techniques is crucial to controlling the outbreak and spread of foodborne diseases. The need for instruments [...] Read more.
In recent years, foodborne disease outbreaks have caused huge losses to the economy and have had severe impacts on public health. The accuracy and variety of detection techniques is crucial to controlling the outbreak and spread of foodborne diseases. The need for instruments increases the difficulty of field detection, while manually-handled samples are subject to user error and subjective interpretation. Here, we use a mini automatic nucleic acid extractor combined with recombinant polymerase amplification (RPA) and lateral flow immunoassay (LFIA) for simultaneous quantitative detection of five major foodborne pathogens. The pre-treatment device using the magnetic bead method allows for nucleic acid extraction of the reagent tank without manual operation, which is highly efficient and stable for preventing aerosol contamination. The nuc gene of Staphylococcus aureus, the toxR gene of Vibrio parahaemolyticus, the rfbE gene of Escherichia coli O157:H7, the hlyA gene of Listeria monocytogenes, and the fimY gene of Salmonella enterica were used as target fragments. The labeled antibody concentration is optimized on the LFIA to find the equilibrium point for the binding capacity of the five chemical markers and to efficiently and accurately visualize the bands. The RPA assay shows an optimal performance at 37 °C for 15 min. The optimized RPA-LFIA detection limit can reach 101 CFU/mL. There was no cross-reactivity among forty-eight strains. Furthermore, the average recoveries in spiked food samples were 90.5–104.5%. In summary, the RPA-LFIA established in this study can detect five pathogenic bacteria simultaneously with little dependence on laboratory equipment, and it has promising prospects for screening in low-resource areas. Full article
(This article belongs to the Special Issue Detection and Epidemiology of Foodborne Pathogens)
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11 pages, 1246 KiB  
Article
Only Low Effects of Water Filters on the Enteric Carriage of Gastrointestinal Pathogen DNA in Colombian Indigenous People
by Simone Kann, Gustavo Concha, Maria Hartmann, Thomas Köller, Juliane Alker, Ulrich Schotte, Lothar Kreienbrock, Hagen Frickmann and Philipp Warnke
Microorganisms 2022, 10(3), 658; https://doi.org/10.3390/microorganisms10030658 - 19 Mar 2022
Cited by 4 | Viewed by 1908
Abstract
Water filtration is a common strategy of water sanitation in resource-poor tropical settings. Here, we assessed the intermediate term effect of this preventive procedure including specific filter-related as well as general hygiene training on the molecular detection of enteric pathogens in stool samples [...] Read more.
Water filtration is a common strategy of water sanitation in resource-poor tropical settings. Here, we assessed the intermediate term effect of this preventive procedure including specific filter-related as well as general hygiene training on the molecular detection of enteric pathogens in stool samples from Colombian Indigenous people. From a total of 89 individuals from an Indigenous tribe called Wiwa, stool samples were assessed by real-time PCR for enteropathogenic microorganisms prior to the implementation of water filtration-based infection prevention. Three years after the onset of the preventive strategy, a follow-up assessment was performed. A significantly beneficial effect of water filtration could be shown for Ascaris spp. only (p = 0.035) and a tendency (p = 0.059) for Hymenolepis nana. No hints for effects on the gastrointestinal shedding of Giardia duodenalis, Entamoeba histolytica, Cryptosporidium spp., Campylobacter spp., Shigella spp./enteroinvasive Escherichia coli, Necator americanus, Strongyloides stercoralis, Trichuris trichiura, and Taenia spp. were seen. In conclusion, the study indicates that water filtration can only be an element of a multi-modal hygiene concept to reduce enteric pathogen carriage in inhabitants of resource-poor tropical settings in spite of tendencies of beneficial effects. Full article
(This article belongs to the Special Issue Detection and Epidemiology of Foodborne Pathogens)
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13 pages, 1008 KiB  
Article
Dietary Inclusion of a Saccharomyces cerevisiae-Derived Postbiotic Is Associated with Lower Salmonella enterica Burden in Broiler Chickens on a Commercial Farm in Honduras
by W. Evan Chaney, S. Ali Naqvi, Manuel Gutierrez, Abel Gernat, Timothy J. Johnson and Derek Petry
Microorganisms 2022, 10(3), 544; https://doi.org/10.3390/microorganisms10030544 - 01 Mar 2022
Cited by 8 | Viewed by 2971
Abstract
Postbiotic feed additives may aid foodborne pathogen reduction during poultry rearing. The study objective was to evaluate a postbiotic additive in parallel to an industry control diet and the subsequent associated burden of Salmonella enterica on a single, commercial broiler farm in Honduras. [...] Read more.
Postbiotic feed additives may aid foodborne pathogen reduction during poultry rearing. The study objective was to evaluate a postbiotic additive in parallel to an industry control diet and the subsequent associated burden of Salmonella enterica on a single, commercial broiler farm in Honduras. Twelve houses were matched and assigned the standard diet (CON) or standard diet plus postbiotic (SCFP). New litter was placed in each house and retained across flock cycles with sampling prior to each chick placement and three consecutive rearing cycles. At ~33–34 days, 25 ceca were collected on-farm from each house, treatment, and cycle. Salmonella prevalence in litter for CON (30.6%) and SCFP (27.8%) were equivalent; however, Salmonella load within positive samples was lower (p = 0.04) for SCFP (3.81 log10 MPN/swab) compared to CON (5.53 log10 MPN/swab). Cecal prevalence of Salmonella was lower (p = 0.0006) in broilers fed SCFP (3.4%) compared to CON (12.2%). Salmonella load within positive ceca were numerically reduced (p = 0.121) by 1.45 log10 MPN/g for SCFP (2.41 log10 MPN/g) over CON (3.86 log10 MPN/g). Estimated burden was lower (p = 0.003) for SCFP flocks (3.80 log10 MPN) compared to CON (7.31 log10 MPN). These data demonstrate the preharvest intervention potential of postbiotics to reduce Salmonella enterica in broiler chickens. Full article
(This article belongs to the Special Issue Detection and Epidemiology of Foodborne Pathogens)
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