Molecular Physiology and Synthetic Biology of Bioenergy-Related Microorganisms

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: closed (30 September 2023) | Viewed by 1081

Special Issue Editor

Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China
Interests: cellulosome; bioenergy; synthetic biology; enzymes; molecular biology; bioenergy-related microorganisms

Special Issue Information

Dear Colleagues,

The biological production of fuels and chemicals is a key process to achieving a circular economy and sustainable development for our world. Many bacteria, microalgae, fungi, etc. have become practical or potential biofuel producers due to their unique physiological and metabolic processes. Most of the naturally isolated microorganisms cannot directly meet the needs of production and need to be further engineered and improved. Understanding the molecular physiological mechanism of these microorganisms can provide new targets and solutions for the engineering of these microorganisms to improve biofuel production. Therefore, the research on the molecular physiological mechanism of bioenergy-related microorganisms is an important basis for their application, and the development of synthetic biology based on this knowledge will generate biofuel production strains. This special issue will provide a platform to display the latest results, progress, and summary of the molecular physiology research of various bioenergy-related microorganisms and the strain development by synthetic biology.

This Special Issue welcomes research articles, reviews, and communications on the following topics:

  • Molecular Physiology and Metabolism of Cellulolytic Microorganisms
  • Molecular Physiology and Metabolism of Hydrogen-producing Microorganisms
  • Molecular Physiology and Metabolism of Methanogenic Microorganisms
  • Molecular Physiology and Metabolism of Lipid-rich Microorganisms
  • Molecular Physiology and Metabolism of Solventogenic Microorganisms
  • Metabolic engineering of the above microorganisms
  • Construction of microbial cell factories for biofuel production
  • Construction of microbial cell factories that produce bio-based chemicals

Prof. Dr. Yingang Feng
Guest Editor

Manuscript Submission Information

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Keywords

  • bioenergy
  • molecular physiology
  • metabolism
  • molecular mechanism
  • metabolic engineering
  • microbial cell factory
  • synthetic biology

Published Papers (1 paper)

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Research

17 pages, 2074 KiB  
Article
The Restriction–Modification Systems of Clostridium carboxidivorans P7
by Patrick Kottenhahn, Gabriele Philipps, Boyke Bunk, Cathrin Spröer and Stefan Jennewein
Microorganisms 2023, 11(12), 2962; https://doi.org/10.3390/microorganisms11122962 - 12 Dec 2023
Viewed by 889
Abstract
Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming industry [...] Read more.
Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming industry waste gases. However, the genetic engineering of this bacterium is hindered by its multiple restriction–modification (RM) systems: the genome of C. carboxidivorans encodes at least ten restriction enzymes and eight methyltransferases (MTases). To gain insight into the complex RM systems of C. carboxidivorans, we analyzed genomic methylation patterns using single-molecule real-time (SMRT) sequencing and bisulfite sequencing. We identified six methylated sequence motifs. To match the methylation sites to the predicted MTases of C. carboxidivorans, we expressed them individually in Escherichia coli for functional characterization. Recognition motifs were identified for all three Type I MTases (CAYNNNNNCTGC/GCAGNNNNNRTG, CCANNNNNNNNTCG/CGANNNNNNNNTGG and GCANNNNNNNTNNCG/CGNNANNNNNNNTGC), two Type II MTases (GATAAT and CRAAAAR), and a single Type III MTase (GAAAT). However, no methylated recognition motif was found for one of the three Type II enzymes. One recognition motif that was methylated in C. carboxidivorans but not in E. coli (AGAAGC) was matched to the remaining Type III MTase through a process of elimination. Understanding these enzymes and the corresponding recognition sites will facilitate the development of genetic tools for C. carboxidivorans that can accelerate the industrial exploitation of this strain. Full article
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