Bacteriophages of Thermophilic 'Bacillus Group' Bacteria

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Environmental Microbiology".

Deadline for manuscript submissions: 15 October 2024 | Viewed by 1563

Special Issue Editors


E-Mail Website
Guest Editor
Department of Molecular Biotechnology, Faculty of Chemistry, University of Gdansk, 80-308 Gdansk, Poland
Interests: genetic engineering technology

E-Mail Website
Guest Editor
Phage Therapy Center, University Center for Applied and Interdisciplinary Research, University of Gdansk, Gdansk, Poland
Interests: biology of bacteriophages; biodiversity of bacteriophages; regulation of bacteriophage development; regulation of phage gene expression; control of phage DNA replication; phage therapy; phages bearing genes of toxins; bacteriophage genomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Bacteriophages of thermophiles, also known as thermophages, have attracted increasing interest due to their important roles in many biogeochemical and ecological processes, as well as biotechnology applications. However, they are underrepresented in the known prokaryotic virosphere, due to the lack of in-depth investigation. Thus, there is considerable potential for the discovery of novel bacteriophage–host systems in various environments. This Special Issue invites works on thermophages and thermophilic ‘Bacillus group’ bacteria, including some moderately thermophilic Bacillus sp. and highly thermophilic Geobacillus sp. and those living under a temperature of approximately 45–70oC. Ecosystems that provide these conditions are diverse and include terrestrial hot springs, deep-sea hydrothermal vents areas, shallow ocean waters above geothermally active beds, human-generated hot waters, hot deserts, compost piles, greenhouse soils, silage, rotting straw, river sludge, stable manure and digested sewage sludge, among others. We aim to collect manuscripts that deal with the discovery of new ‘Bacillus group’ thermophages, their ecology, genomics, proteomics, molecular biology and biotechnology applications. We also welcome manuscripts that deal wih the comparative deciphering of the molecular basis of the thermophages’ biology and analysis of the environmental aspects of the thermophages’ effect on the thermophile community. The majority of detected bacteriophages of the ‘Bacillus group’ belong to the Siphovus morphotype, and others belong to the Myovirus and Podovirus morphotypes. All of their genomes are composed of dsDNA, either linear, circular or circularly permuted. Undoubtedly, with increased research, greater ‘Bacillus group’ thermophage diversification is expected.

Prof. Dr. Piotr Skowron
Prof. Dr. Alicja Wegrzyn
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacteriophages
  • thermophilic bacteria
  • Bacillus group
  • phage therapy
  • host specificity
  • genome sequencing
  • viral ecology
  • molecular biology
  • biotechnology
  • microbial diversity

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Other

9 pages, 1380 KiB  
Protocol
A Method for Rapid Polyethyleneimine-Based Purification of Bacteriophage-Expressed Proteins from Diluted Crude Lysates, Exemplified by Thermostable TP-84 Depolymerase
by Beata Łubkowska, Edyta Czajkowska, Ireneusz Sobolewski, Natalia Krawczun, Agnieszka Żylicz-Stachula and Piotr M. Skowron
Microorganisms 2023, 11(9), 2340; https://doi.org/10.3390/microorganisms11092340 - 19 Sep 2023
Viewed by 1259
Abstract
Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting thermophilic Geobacillus stearothermophilus bacteria, as a representative enzyme to [...] Read more.
Purification of bacteriophage-expressed proteins poses methodological difficulties associated with the need to process entire culture medium volume upon bacteriophage-induced bacterial cell lysis. We have used novel capsule glycosylase-depolymerase (TP84_26 GD) from bacteriophage TP-84, infecting thermophilic Geobacillus stearothermophilus bacteria, as a representative enzyme to develop a method for rapid concentration and purification of the enzyme present in diluted crude host cell lysate. A novel variant of the polyethyleneimine (PEI)-based purification method was devised that offers a fast and effective approach for handling PEI-facilitated purification of bacteriophage-expressed native proteins. Due to the very basic nature of PEI, the method is suitable for proteins interacting with nucleic acids or acidic proteins, where either mixed PEI-DNA or RNA–protein complexes or PEI–acidic protein complexes are reversibly precipitated. (i) The method is of general use, applicable with minor modifications to a variety of bacteriophage cell lysates and proteins. (ii) In the example application, TP84_26 GD was highly purified (over 50%) in a single PEI step; subsequent chromatography yielded a homogeneous enzyme. (iii) The enzyme’s properties were examined, revealing the presence of three distinct forms of the TP84_26 GD. These forms included soluble, unbound proteins found in host cell lysate, as well as an integrated form within the TP-84 virion. Full article
(This article belongs to the Special Issue Bacteriophages of Thermophilic 'Bacillus Group' Bacteria)
Show Figures

Figure 1

Back to TopTop