Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
6.4
4.5
Journals
Microorganisms
Special Issues
Molecular Analysis of Plant Pathogenic Bacteria, 2nd Edition
share announcement

Special Issue "Molecular Analysis of Plant Pathogenic Bacteria, 2nd Edition"

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Plant Microbe Interactions".

Deadline for manuscript submissions: 15 February 2024 | Viewed by 807

Special Issue Editor

Dr. Toni A. Chapman

E-Mail Website
Guest Editor
Elizabeth Macarthur Agricultural Institute, Menangle 2568, Australia
Interests: plant bacteriology; diagnostics for plant pathogenic bacteria; microbial ecosystems; biosecurity; bacterial diagnostics and genomics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is the continuation of our previous Special Issue, “Molecular Analysis of Plant Pathogenic Bacteria” (https://www.mdpi.com/journal/microorganisms/special_issues/plant_pathogenic_bacteria).

Bacterial taxonomy is currently in a state of flux. As we learn more about bacteria through the application of molecular techniques, the accuracy of taxonomic identification can come into question. What type of analysis will suffice for an identification down to the subspecies level? What techniques are being used to separate species and subspecies within the different plant genera? What are we using to investigate pathogenicity, and is this translating to disease severity in plants? How is the use of molecular techniques helping us to better understand bacterial pathogens? In this Special Issue, we plan to explore the use of molecular techniques to correctly identify bacterial pathogens and pathogenicity.

Dr. Toni A. Chapman
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bacterial taxonomy
  • pathogenicity bacterial
  • pathogens

Related Special Issue

Published Papers (1 paper)

Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Research

17 pages, 2877 KiB  
Article
Rapid Detection and Quantification of Viable Cells of Pectobacterium brasiliense Using Propidium Monoazide Combined with Real-Time PCR
by
Junhui Li
,
Ruxing Chen
,
Ruwei Yang
,
Xinchen Wei
,
Hua Xie
,
Yanxia Shi
,
Xuewen Xie
,
Ali Chai
,
Tengfei Fan
,
Baoju Li
and
Lei Li
Microorganisms 2023, 11(11), 2808; https://doi.org/10.3390/microorganisms11112808 - 19 Nov 2023
Viewed by 442
Abstract
Pectobacterium brasiliense (Pbr) has caused significant economic losses in major vegetable production areas in Northern China by causing bacterial soft rot in cash crops such as potatoes and cucumbers. This study aimed to establish a PMA-qPCR detection method for Pbr by [...] Read more.
Pectobacterium brasiliense (Pbr) has caused significant economic losses in major vegetable production areas in Northern China by causing bacterial soft rot in cash crops such as potatoes and cucumbers. This study aimed to establish a PMA-qPCR detection method for Pbr by screening specific and sensitive primers based on the glu gene and the conserved region of the 23S rRNA gene. Based on the optimized PMA pretreatment conditions, a standard curve was designed and constructed for PMA-qPCR detection (y = −3.391x + 36.28; R2 = 0.99). The amplification efficiency reached 97%, and the lowest detection limit of viable cells was approximately 2 × 102 CFU·mL−1. The feasibility of the PMA-qPCR method was confirmed through a manually simulated viable/dead cell assay under various concentrations. The analysis of potato tubers and cucumber seeds revealed that nine naturally collected seed samples contained a range from 102 to 104 CFU·g−1 viable Pbr bacteria. Furthermore, the system effectively identified changes in the number of pathogenic bacteria in cucumber and potato leaves affected by soft rot throughout the disease period. Overall, the detection and prevention of bacterial soft rot caused by Pbr is crucial. Full article
(This article belongs to the Special Issue Molecular Analysis of Plant Pathogenic Bacteria, 2nd Edition)
►▼ Show Figures

Figure 1

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-1
Back to TopTop