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Micromachines
Special Issues
Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications
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Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B1: Biosensors".

Deadline for manuscript submissions: closed (30 June 2023) | Viewed by 7536

Special Issue Editors

Prof. Dr. Yen-Wen Lu

E-Mail Website
Guest Editor
Department of Biomechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
Interests: digital microfluidics; DNA sensors; blood engineering
Special Issues, Collections and Topics in MDPI journals
Dr. Hsiao-Mei Wu

E-Mail Website
Guest Editor
Department of Biomechatronics Engineering, National Taiwan University, Taipei 10617, Taiwan
Interests: optomechatronics; bio-micro electro mechanical system (MEMS); fluorescence lifetime image detection system; optical interferometer system

Special Issue Information

Dear Colleagues,

Biosensors, which can convert biological responses into electrical signals, have been effectively developed and applied in various fields, such as drug discovery, disease diagnosis, biomedicine, environmental monitoring, and food quality inspection. Recently, the desire of using biosensors as fast diagnostics for quicker and more sensitive detection has been elevated unprecedentedly due to the rapid community spread of the human coronavirus. It is therefore of paramount importance for scientists to enhance the efficiency and accuracy of biosensors, and further, increase the applications and market for this technology. Through integrating multifaceted approaches to design biosensors, researchers have the potential to break through current limitations of the biosensor technology. In light of this, the Special Issue, “Biosensors in Monitoring and Diagnosis for Medical and Agriculture Applications”, is a space for introducing novel technology of biosensors and their applications in all areas, such as the development of biosensors interfaces, the new materials of biosensors, the innovative design of integrated biosensor systems, and modern signal reading mechanisms of biosensors. In this Special Issue, we invite the publication of original research articles and review papers on a wide range of biosensors applications, including in biomedicine, biomedical diagnosis, environmental monitoring, agriculture, and the food industry.

We look forward to receiving your submissions!

Prof. Dr. Yen-Wen Lu
Dr. Hsiao-Mei Wu
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Micromachines is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • electrochemical biosensors
  • optical biosensors
  • microfluidic-integrated biosensors
  • nanomaterial-based biosensors
  • enzyme-based biosensor
  • immunosensors
  • aptamer or nucleic acid-based biosensors
  • microbial or whole-cell biosensors
  • machine learning

Published Papers (5 papers)

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Research

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10 pages, 2451 KiB  
Article
Virtual Multiplexing Chamber-Based Digital PCR for Camel Milk Authentication Applications
by Jinchao Li, Jingmeng Cheng, Shanshan Li, Jie Jayne Wu and Junwei Li
Micromachines 2023, 14(8), 1619; https://doi.org/10.3390/mi14081619 - 17 Aug 2023
Viewed by 870
Abstract
In this work, we proposed a chamber-based digital PCR (cdPCR) microfluidic device that is compatible with fluorescence imaging systems for milk adulteration detection. The device enables the digitalization of PCR reagents, which are loaded into microchambers, and subsequent thermocycling for DNA amplification. Then, [...] Read more.
In this work, we proposed a chamber-based digital PCR (cdPCR) microfluidic device that is compatible with fluorescence imaging systems for milk adulteration detection. The device enables the digitalization of PCR reagents, which are loaded into microchambers, and subsequent thermocycling for DNA amplification. Then, fluorescence images of the microchambers are captured and analyzed to obtain the total number of positive chambers, which is used to calculate the copy numbers of the target DNA, enabling accurate quantitative detections to determine intentional milk adulteration from accidental contaminations. The validation of this device is performed by camel milk authentication. We performed 25,600-chamber virtual multiplexing cdPCR tests using 40 × 40 chamber devices for the detection of DNA templates extracted from pure or mixed milk with different dilutions. Then, the cdPCR chip was used to authenticate blind milk samples, demonstrating its efficacy in real biotechnical applications. Full article
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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19 pages, 4072 KiB  
Article
Analysis of Polarization Images in the Microphysical Blood Parameters Research for the Hematocrit Diagnostics
by Ruslan D. Khlynov, Victoria A. Ryzhova, Sergey N. Yarishev, Igor A. Konyakhin, Valery V. Korotaev, Yuri E. Shelepin, Todor S. Djamiykov and Marin B. Marinov
Micromachines 2022, 13(12), 2241; https://doi.org/10.3390/mi13122241 - 16 Dec 2022
Cited by 1 | Viewed by 1119
Abstract
The development of non-invasive optoelectronic technologies for human blood monitoring is one of the important research areas for medicine. A critical analysis of optoelectronic methods of blood research and the micromechanical systems based on them is carried out in this article. A design [...] Read more.
The development of non-invasive optoelectronic technologies for human blood monitoring is one of the important research areas for medicine. A critical analysis of optoelectronic methods of blood research and the micromechanical systems based on them is carried out in this article. A design realization of a polarizing portable system for non-invasive monitoring of hematocrit as one of the basic homeostatic constants of the human body containing information about the microphysical parameters of blood cells has been substantiated. A physical model of polarized radiation conversion in a video information system of laser sensing of a biological research object has been formed. Visual and quantitative differences in the spatial distribution of polarization parameters of the scattered radiation for the states of the body with different hematocrit levels have been revealed. A scheme of a multichannel imaging portable system, based on a smartphone using miniature optical and microelectronic components of information conversion for non-invasive monitoring of microphysical blood parameters, has been created. The system implements the principle of polarimetric blood photometry and a multiparametric analysis of the polarization properties of the laser radiation scattered by blood. The developed portable optoelectronic system, based on a smartphone, can be used for rapid blood diagnostics in disaster medicine and the presence of clinical contraindications to the formation of invasive tests. The proposed polarization-based approach is a promising automated alternative to traditional devices and systems for the research of microphysical blood parameters. Full article
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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16 pages, 3701 KiB  
Article
Rapid Detection of Lipopolysaccharide and Whole Cells of Francisella tularensis Based on Agglutination of Antibody-Coated Gold Nanoparticles and Colorimetric Registration
by Nadezhda A. Byzova, Anatoly V. Zherdev, Alexey A. Gorbatov, Anton G. Shevyakov, Sergey F. Biketov and Boris B. Dzantiev
Micromachines 2022, 13(12), 2194; https://doi.org/10.3390/mi13122194 - 11 Dec 2022
Cited by 1 | Viewed by 1204
Abstract
The paper presents development and characterization of a new bioanalytical test system for rapid detection of lipopolysaccharide (LPS) and whole cells of Francisella tularensis, a causative agent of tularemia, in water samples. Gold nanoparticles (AuNPs) coated by the obtained anti-LPS monoclonal antibodies [...] Read more.
The paper presents development and characterization of a new bioanalytical test system for rapid detection of lipopolysaccharide (LPS) and whole cells of Francisella tularensis, a causative agent of tularemia, in water samples. Gold nanoparticles (AuNPs) coated by the obtained anti-LPS monoclonal antibodies were used for the assay. Their contact with antigen in tested samples leads to aggregation with a shift of absorption spectra from red to blue. Photometric measurements at 530 nm indicated the analyte presence. Three preparations of AuNPs with different diameters were compared, and the AuNPs having average diameter of 34 nm were found to be optimal. The assay is implemented in 20 min and is characterized by detection limits equal to 40 ng/mL for LPS and 3 × 104 CFU/mL for whole cells of F. tularensis. Thus, the proposed simple one-step assay integrates sensitivity comparable with other immunoassay of microorganisms and rapidity. Selectivity of the assay for different strains of F. tularensis was tested and the possibility to choose its variants with the use of different antibodies to distinguish virulent and non-virulent strains or to detect both kinds of F. tularensis was found. The test system has been successfully implemented to reveal the analyte in natural and tap water samples without the loss of sensitivity. Full article
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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11 pages, 2839 KiB  
Article
Multiple Biomarker Simultaneous Detection in Serum via a Nanomaterial-Functionalized Biosensor for Ovarian Tumor/Cancer Diagnosis
by Yu Wu, Chunhua Wang, Chao Wang, Pan Wang, Xiaohan Chang, Lin Han and Yu Zhang
Micromachines 2022, 13(12), 2046; https://doi.org/10.3390/mi13122046 - 22 Nov 2022
Cited by 6 | Viewed by 1620
Abstract
Ovarian tumors/cancers are threatening women’s health worldwide, which demands high-performance detection methods and accurate strategies to effectively detect, diagnose and treat them. Here, we report a nanographene oxide particle-functionalized microfluidic fluorescence biosensor to simultaneously detect four biomarkers, CA125, HE4, CEA and APF, for [...] Read more.
Ovarian tumors/cancers are threatening women’s health worldwide, which demands high-performance detection methods and accurate strategies to effectively detect, diagnose and treat them. Here, we report a nanographene oxide particle-functionalized microfluidic fluorescence biosensor to simultaneously detect four biomarkers, CA125, HE4, CEA and APF, for ovarian tumor/cancer diagnosis. The developed biosensor exhibits good selectivity and a large biomarker detection range with a limit of detection of 0.01 U/mL for CA125 and ~1 pg/mL for HE4, CEA and APF. The current results indicate that (1) the proposed biosensor is a promising tool for the simultaneous detection of multiple biomarkers in ovarian tumors/cancer and (2) CA125 and HE4 are strong indicators, AFP may be helpful, and CEA is a weak biomarker for ovarian tumor/cancer diagnosis. The proposed biosensor would be a potential tool, and an analytical approach for the simultaneous detection of multiple biomarkers will provide a new strategy for the early screening, diagnosis and treatment of ovarian tumors/cancers, as well as other cancers. Full article
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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Review

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18 pages, 5321 KiB  
Review
Silicon Carbide-Based DNA Sensing Technologies
by Abdulla Al Mamun, Mason McGarrity, Jong-Hoon Kim and Feng Zhao
Micromachines 2023, 14(8), 1557; https://doi.org/10.3390/mi14081557 - 04 Aug 2023
Cited by 1 | Viewed by 1305
Abstract
DNA sensing is critical in various applications such as the early diagnosis of diseases and the investigation of forensic evidence, food processing, agriculture, environmental protection, etc. As a wide-bandgap semiconductor with excellent chemical, physical, electrical, and biocompatible properties, silicon carbide (SiC) is a [...] Read more.
DNA sensing is critical in various applications such as the early diagnosis of diseases and the investigation of forensic evidence, food processing, agriculture, environmental protection, etc. As a wide-bandgap semiconductor with excellent chemical, physical, electrical, and biocompatible properties, silicon carbide (SiC) is a promising material for DNA sensors. In recent years, a variety of SiC-based DNA-sensing technologies have been reported, such as nanoparticles and quantum dots, nanowires, nanopillars, and nanowire-based field-effect-transistors, etc. This article aims to provide a review of SiC-based DNA sensing technologies, their functions, and testing results. Full article
(This article belongs to the Special Issue Biosensors in Monitoring and Diagnosis for Medical and Agricultural Applications)
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